Ultraviolet radiation of skin leads to a systemic alteratior tkat inhibits the normal pattern of immunologic tumor rejection., suppresses the contact hypersemisivity and transiently alters the morphology and the surface marker characteristics of Langerhans cells. Moreover, Ultraviolet radiation elaborates the ETAF, neuropeptides, proteins, and urocaicacid which may alter immunologic responses. But no other study about the effects of UVB irradiation on the systemic immunologic functions of the macrophages of internal organs was reported. The macrophages of the reticuloendothelial system (RES) play a central role in cell-mediated immunity, because they are involved both in the initiation of responses as antigen-presenting, cells, and in the effector phase as inflammatory, tumoricidal and microbicidal cells. The present study was intended to investigate the effects of UVB irradiation on the immunologic functions of mouse peritoneal macrophages. Normal 6-8-week-old BALB/c mice were exposed at the dose rate of 20mJ/cm and 40mJ/cm of UVB per day, 5 days per weeks for 2 weeks, 4 weeks and 8 weeks. Then the peritoneal macrophages were obtained from the mice and the changes of cell count, chemotactic index, phagocytic index, NBT reduction rate and superoxide (0) production were examined. The results were as follows : 1)the number of mouse peritoneal macrophages was decreased by UVB radiation, 2) the chemotactic index of mouse peritoneal macrophage was not altered by UVB radiation, 3) phagocytic activity of mouse pertoneal macrophage was significsntly decreased by UBV radiation, 4) NBT reduction rate in mouse aeritoneal macrophage after UVB radiation was sinificanily decreased in all experimental group, and 5) Superoxide (0) production in mouse peritoneal macrophage after UVB radiation was decreased in all experimental groups.
Animals ; Cell Count ; Immunity, Cellular ; Langerhans Cells ; Macrophages ; Macrophages, Peritoneal* ; Mice* ; Mononuclear Phagocyte System ; Neuropeptides ; Skin ; Superoxides

BACKGROUND: Previous studies have shown that in vitro exposure to ultraviolet B(UVB) radiation resulted in a dose-dependent inhibition of natural killer activity(NK activity) of normal human peripheral blood mononuclear cella(PBMC), and that in vivo exposure to snlight also induced NK activity suppression. The precise meehanism of the UV-regulation on the riat iral killer system(NK system) is not established. Objective & METHOD: The purpose of this study is to examine whether the addition of interleukin-2(IL-2) and/or free oxygen radical scavengers, superoxide dismutas(SOD) or sodium azide(SA), is effective in reducing the UVJ3-induced suppression of NK activity of FBMC. RESULTS: The results are as follows 1. The suppressive effect of UVB radiat,ion on NK activity could successfully be prevented in the presence of SOD(100 and 1,000U/ml) during the radiation. 2. SA( LO and 10 M/ml) did not prevent the suppression of NK activity. 3. IL-2(100U/ml) markedly enhanced the NK activity of nonirradiated PBMC, but had no effect on irradiated PBMC. 4. Combination treatment with both IL-2 and free radical scavengers on UVB-irradiated PBMC resulted in no additive or synergistic effect on the prevention of the suppression of NK activity compared with a single treatment with either IL-2 or free radical scavengers. CONCLUSION: In the presserit study, we found that SOD providec a protective effect on NK activity during the UVB radiation and we suggest that superoxide anion(O ) might play a major role in the UV-regulatory mechanisms of the NK system.
Free Radical Scavengers ; Humans* ; Interleukin-2* ; Oxygen ; Sodium ; Superoxides

No abstract available.
Carcinogenesis* ; Humans* ; Oncogenes*

The efficacy of the fine needle aspiration biopsy and cytological examination (FNABC) under ultrasonographic (US) guides for the diagnosis of cervical tuberculous lymphadenitis was assessed. In one hundred and one patients with a neck mass or masses, tuberculous lymphadenitis has been proved by FNABC. US disclosed the physical characteristics of the masses: All the cases that involved two or more lymph nodes were multiple lesions. Eighty lesions (79.1%) were multiregional, and in 19 cases (18.8%), bilateral neck was involved. The posterior triangle of the neck was the most prevalent site (N = 69, 68.3%). Ninety two cases (90.9%) were hypoechoic lesions and 9 (9.1%) showed mixed echo patterns. There was no hyperechoic lesion (p< 0.001). The sensitivity of FNABC was 77.2% and the specificity was 99.0%. The diagnostic accuracy was 85.0%. There was no complication during the procedure. FNABC for the diagnosis of cervical tuberculous lymphadenitis is a safe, convenient procedure and has a relatively high specificity. The limitation of FNABC, the low sensitivity, seemed to be compensated by US examinations.
Biopsy, Needle/*methods ; Human ; Neck ; Tuberculosis, Lymph Node/*pathology/*ultrasonography

BACKGROUND: Two types of local reactions due to cutaneous administration of vitamin K. eczematoid, and indurated pilaque and localized scleroderma, have been described. In the acute phase, a generalized maculopapular eruption (Id reation) may accompany either reaction. Liver disease has been reported with vitamir K hypersensitivity but the mechanism of vitamin K dermatitis is unknown. OBJECTIVE & MEHTODS: In 10 of vitamin K dermatitis patients, we studied the clinical features, histopathologic findings, and provocation tests (patch test & intrader nal test). RESULTS: 1. All patients had localized erythematous plaque but none had sclerodermoid skin eruption. Four patients were associated with Id reaction. 2. The onsets of eruptions after initial injection of vitamin K were within one week (one case), 2 to 3weeks(seven cases), or 3 weeks (two cases), and the doses of administered vitamin K were between 30 and 310 mg. 3. Four had liver diseases and 5 had blood eosinophilia. 4. Of 7 patients who had patch-and intradermal test, intradermal test showed all positive at either day 2 or day 4 but patch test were all negative. 5. The histopathologic findings of all the cases showed perivascuiiar and diffuse infiltrations of numeroas osinophils and mononuclear cells and one case showed panniulitis. CONCLUSION: The cell-mediated immune reaction may play a role on the pathogenesis of vitamin K, dermatitis. Liver dysfuncticn and/or another factors may be a precivitating factor of vitamin K dermatitis, and intradermal the seems to be more useful in the diagnoiis of vitamin K dermatitis than patch test.
Administration, Cutaneous ; Dermatitis* ; Eosinophilia ; Humans ; Hypersensitivity ; Intradermal Tests ; Liver ; Liver Diseases ; Patch Tests ; Scleroderma, Localized ; Skin ; Vitamin K ; Vitamin K 1* ; Vitamins*

We reviewed the cliniccipat.hologic findings of 11 cases of dermatophytic granulomas. Patients consisted of 8 male and 3 females with a mean age of 45 years (range 5-80 years). The symptom was neither painful nor pruritic except one case. Skin lesions mimicked tuberculosis (2 cases), sporotriehosis (2 cases), calcinosis cutis (1 case) or eczema (1 case). The commonest sites involved were upper extremities, followed by legs, cace and scalp. The duration between the onset of the diseases and final diagnosis ranged 1 month to 3 years. Possible predisposing factors causing dermatophytic granulomas appeared to be due to application of steroid ointment (6 cases), diabetes mellitus (2 cases) and trauma (1 case). Histopathologic featured revealed suppurative folliculitis with fo eign body granuloma in most cases and lobular ponniculitis in 2 cases. With PAS and methenamine silver stains, hyphae and spores could be identified on the horny layers, within hair follicles and/or on the dermis in biopsy specimen.', of all cases. Culture of the causativi; organisms using dermal fragment on abouraud media demon-strated T. rubrum in seven patients and M. canis in two. A test for recall antigen(Multitest CMI) showed suppression of cellular immunity in 3 cases. Treatment with griseofulvin or ketoconazole for 2-10 weeks was effective except one case.
Biopsy ; Calcinosis ; Causality ; Coloring Agents ; Dermis ; Diabetes Mellitus ; Diagnosis ; Eczema ; Female ; Folliculitis ; Granuloma* ; Griseofulvin ; Hair Follicle ; Humans ; Hyphae ; Immunity, Cellular ; Ketoconazole ; Leg ; Male ; Methenamine ; Scalp ; Skin ; Spores ; Tuberculosis ; Upper Extremity

No abstract available.
Craniopharyngioma* ; Magnetic Resonance Imaging*

Nuclear DNA content in 31 cases of thyroid neoplasm was determined by flow cytometry with the use of paraffin-embedded archival tissue. DNA aneuploidy was found in 6 cases (19.4%) of the 31 thyroid neoplasms; such as 2 of 8 (DI=1.16, 1.56) follicular adenomas, 1 of 6 (DI=1.10) follicular carcinomas, 1 of 15 (DI=1.18) papillary carcinomas and 2 of 2 (DI=1.76, 2.07) medullary carcinomas. The remaining tumors were diploid. No significant difference between follicular adenomas and carcinomas was detected with respect to the S phase fraction(SPF). In the papillary carcinoma group the SPF was higher than in the follicular neoplasm group, but it was statistically insignificant. Regional lymph node metastasis was present in 8 of 15( 53.3%) papillary carcinomas but absent in all of the 14 follicular neoplasms. In the medullary carcinoma group one case showed regional node metastasis at the time of resection and the other developed metastasis 11 months after surgical removal of the primary lesion. In this study tumors predominantly composed of Hurthle cells were found to have a significantly higher D.I. than those with few or no Hurthle cells. No significant difference was found between tumors with metastasis and those without metastasis.
Adenoma ; Neoplasm Metastasis

Our aim was to identify histologic changes in cervical carcinoma tumor cells due to chemotherapy,by electron microscopic examination. Cisplatin and 5-fluorouracil were used between March 1, 1994 and February 28, 1995 on a total of 16 patients. The treatment schedule consisted of 75 mg/m2 cisplatin via iv with hyperhydration on Day 1, in combination with 1000 mg/m2 5-fluorouracil via continuous iv on Day 1 to 5. The treatment was repeated every 4 weeks with a maxinum of 3 courses. Ultrastructurally, chemotherapy induced apoptosis,indicated by condensation of nucleus and cytoplasm,fragmentation of nuclei, and apoptotic bodies. Due to repeat chemotherapy, increased levels of desmosomes were noted and parts of tumor nests were replaced by mature squamous cells. Tumor nests were reduced in size, mitochondria exhibited swelling, the endoplasmic reticulum was dilated, and infiltration of inflammatory cells increased after chemotherapy. These results suggest that chemotherapy induces apoptosis and injury to the cytoplasm and nuclei of tumor cells. However, the exact selection mechanism of the biochemical pathway of cell death is not fuUy understood. Further study of the molecular biologic actions of cell death after chemotherapy is warranted.
Apoptosis ; Appointments and Schedules ; Cell Death ; Cisplatin ; Cytoplasm ; Desmosomes ; Drug Therapy* ; Endoplasmic Reticulum ; Fluorouracil ; Humans ; Mitochondrial Size

Idiopathic gingival fibromatosis is associated with other abnorma ities of which the most striking is hypertrichosis of variable degree. Gorlin and Pindborg described this condition as a distinct syndrome termed idiopathic gingival fibromatosis and hypertrichosis. We report a case of idiopathic gingival fibromatosis and hypertrichosis occuring in 34-year-old woman with enlarged gingival tissue, hypertrichosis, and enlargenment of the breasts, ears and nose. Laboratory tests including hormonal and chromosomal studies were all within normal limits. Histologically, the gingival hyperplastic tissue showed thick bundle of collagen fibers with few fibroblasts or capillaries and little or no inflammatory infiltrate.
Adult ; Breast ; Capillaries ; Collagen ; Ear ; Female ; Fibroblasts ; Fibromatosis, Gingival* ; Humans ; Hypertrichosis* ; Nose ; Strikes, Employee