1.Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor-beta1 Type II Receptor in Oral Leukoplakia and Squamous Cell Carcinoma.
Tae Yeon KIM ; Jong In YOOK ; Jin KIM
Korean Journal of Pathology 1997;31(12):1247-1255
Growth stimulatory/inhibitory factors and their receptors are the important mediators of control of epithelial cell proliferation and differentiation. The aim of this study was to observe the distribution of epidermal growth factor receptor (EGFR) and transforming growth factor-beta1 type II receptor (TbetaRII) during carcinogenesis of oral squamous cell carcinoma (OSCC). Formalin fixed, paraffin embedded tissue from 25 oral leukoplakias (OL) and 15 OSCC was immunostained by avidin-biotin complex method. In OSCC, the carcinomatous area and the adjacent dysplastic/ hyperplastic area were examined. In OL, the hyperplasia and the epithelial dysplasia were examined. Monoclonal anti-EGFR Ab and polyclonal anti-TbetaRII Ab were applied. EGFR was mainly expressed in the basal layer and was increased with epithelial dysplasia in OL. TbetaRII was not detected in the basal cell layer and dysplastic area in OL. In contrast, the dysplastic area adjacent to OSCC showed positivity in the entire layer including the dysplastic area. In all cases of OSCC, both EGFR and TbetaRII showed positive reactions. EGFR was increased with the progression to the malignancy, and the expression pattern of TbetaR II was altered to be positive in the basal cell layer with progression to malignancy. These results suggest that the expression of EGFR appeared to be an early event and TbetaR II may be related to malignant transformation during oral carcinogenesis. The expression pattern of EGFR and TbetaR II may contribute to predict the risk of the development of carcinoma in oral premalignant lesions.
Carcinogenesis
;
Carcinoma, Squamous Cell*
;
Epidermal Growth Factor*
;
Epithelial Cells
;
Formaldehyde
;
Hyperplasia
;
Leukoplakia, Oral*
;
Paraffin
;
Receptor, Epidermal Growth Factor*
2.A Case of Dyskeratosis Congenita in a Woman.
Chi Yeon KIM ; Tae Heung KIM ; Tae Jin YOON
Korean Journal of Dermatology 2001;39(10):1162-1165
No abstract available.
Dyskeratosis Congenita*
;
Female
;
Humans
3.A clinical & statistical analysis of the facial bone fractures.
Jin Dong KIM ; Tae Yeon KIM ; Chun Eun CHUNG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(1):38-47
No abstract available.
Facial Bones*
4.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
5.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
6.Successful Treatment of Pyoderma Gangrenosum with Prednisolone, Cyclosporine, and Dapsone.
Kyong Tae KIM ; Chi Yeon KIM ; Tae Jin YOON
Korean Journal of Dermatology 2006;44(3):372-374
Pyoderma gangrenosum is an uncommon, chronic, recurrent cutaneous ulcerative disease. It is difficult to diagnosis and is frequently associated with systemic diseases. There is no specific therapy, nor effective uniform therapy. Systemic treatment with corticosteroids or cyclosporine, alone or together, appeared to be effective in many cases. However, prolonged therapy is associated with significant side effects, and in refractory cases, immunomodulatory therapy is needed. We report a case of pyoderma gangrenosum, which developed in a 41-years-old woman, who showed no symptoms of having an underlying systemic disease. The conduction was successfully treated with prednisolone, cyclosporine and dapsone.
Adrenal Cortex Hormones
;
Cyclosporine*
;
Dapsone*
;
Diagnosis
;
Female
;
Humans
;
Immunomodulation
;
Prednisolone*
;
Pyoderma Gangrenosum*
;
Pyoderma*
;
Ulcer
7.Successful Treatment of Pyoderma Gangrenosum with Prednisolone, Cyclosporine, and Dapsone.
Kyong Tae KIM ; Chi Yeon KIM ; Tae Jin YOON
Korean Journal of Dermatology 2006;44(3):372-374
Pyoderma gangrenosum is an uncommon, chronic, recurrent cutaneous ulcerative disease. It is difficult to diagnosis and is frequently associated with systemic diseases. There is no specific therapy, nor effective uniform therapy. Systemic treatment with corticosteroids or cyclosporine, alone or together, appeared to be effective in many cases. However, prolonged therapy is associated with significant side effects, and in refractory cases, immunomodulatory therapy is needed. We report a case of pyoderma gangrenosum, which developed in a 41-years-old woman, who showed no symptoms of having an underlying systemic disease. The conduction was successfully treated with prednisolone, cyclosporine and dapsone.
Adrenal Cortex Hormones
;
Cyclosporine*
;
Dapsone*
;
Diagnosis
;
Female
;
Humans
;
Immunomodulation
;
Prednisolone*
;
Pyoderma Gangrenosum*
;
Pyoderma*
;
Ulcer
8.An experimental study of cartilage growth in autogenous transplantation of young rabbit ear.
Taek Keun KWON ; Tae Yeon KIM ; Dong Jin LEE ; Chun Eun CHUNG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(3):411-419
No abstract available.
Cartilage*
;
Ear*
9.Weaning Following a 30 Minutes Spontaneous Breathing Trial.
Jin SHIN ; Young Min KOH ; Yeon Tae CHUNG
Tuberculosis and Respiratory Diseases 1997;44(6):1326-1331
BACKGROUND: Weaning is the process of switching a patient from mechanical ventilator to spontaneous breathing. A number of different weaning techniques can be employed. At recent study, conventional spontaneous breathing trial was superior to other techniques, such as intermittent mandatory ventilation(IMV) or pressure support ventiation(PSV). But adequate observation time of the spontaneous breathing trial was not determined. We reported the effectiveness of weaning and extubation following a 60 minutes spontaneous breathing trial with simple oxygen supply through the endotrachial tube. In this study, we tried to shorten the spontaneous breathing time from 60 minutes to 30 minutes. If weaning success was predicted after 30 minutes spontaneous breathing, extubation was done without reconnection with ventilator. METHODES: Subjects consisted of 42 mechanically ventilated patients from August 1994 to July 1995. The weaning trial was done when the patients recovered sufficiently from respiratory failure that originally required ventilatory assistance, the patients became alert and showed stable vital sign, and arterial O2 tension was adequated(PaO2>55 mmHg) with less than 40% of inspired oxygen fraction. We conducted a careful physical examination when the patients was breathing spontaneously through the endobronchial tube for 30 minutes. We terminated the trial if a patients was any of following signs of distress; cyanosis, diaphoresis, tachypnea(above 30 breaths per minute), and extreme tachycardia. Patients who had none of this features during spontaneous breathing for 30 minutes were extubated promptly. RESULT: 17 weaning trials of 15 patients were done in 42 mechanically ventilated patients. Successful weaning and extubation was possible in 14 trials of total 17 trials. In this 14 patients, 8 patients were extubated after 30 minutes spontaneous breathing, 3 patients were extubated after 60 minutes spontaneous breathing, and 3 patients needed over 3 hours for extubation from weaning. We found similar overall success rate compared with weaning following a 60 minutes spontaneous breathing trial. CONCLUISON: From the result of present study, we believe that weaning and extubation from mechanical ventilation following a 30 minutes spontaneous breathing with 02 supply through the endotracheal tube is a simple and effective method
Cyanosis
;
Humans
;
Oxygen
;
Physical Examination
;
Respiration*
;
Respiration, Artificial
;
Respiratory Insufficiency
;
Tachycardia
;
Ventilators, Mechanical
;
Vital Signs
;
Weaning*
10.Cauda Equina Syndrome in the Lumbar Disc Herniation.
Hong Tae KIM ; Soon Man HONG ; Keun Il LEE ; Jin Wook JUNG ; Yeon Min PARK
Journal of Korean Society of Spine Surgery 1998;5(1):116-121
STUDY DESIGN: A retrospective analysis of the patients who had cauda equina syndrome caused by a herniated lumbar disc. OBJECTIVES: To assess the clinical debates concerning the diagnosis, treatment, and results of treatment. SUMMARY OF LITERATURE REVIEW: This syndrome has been considered as an absolute indication of surgical treatment in the herniated lumbar disc and poor prognosis after surgery were reported. Although there are debates on the timing of surgery, early recognition early surgical treatment are recommended for a better results of treatment. MATERIALS AND METHODS: 23 consecutive patients having a cauda equina syndrome caused by herniated lumbar disc were reviewed retrospectively. They were 14 males and 9 females having ages of 41.6 in average. The incidence of this syndrome was 3.8% of the patients who had surgical treatment of herniated lumbar disc. RESULTS: All patients had bladder dysfunction in addition to the low back pain and radicular pain in the leg, but five patient did not recognize their urinary retention which were shaded by severe leg pain. A surgical treatment were performed for all patients at 6.8 days in average after onset of the bladder dysfunction. At follow-up of 3 years and 3 months in average after surgery, 20 patients(86.9%) had satisfactory resolution of the low back pain and leg pain, and all patients had complete recovery of motor weakness except one patients who had residual foot drop. Bladder function recovered satisfactorily in 18 patients (78.3%), but 4 patients had some difficulty of urination and one patient needed a sphinterotomy for urination. CONCLUSIONS: The cauda equine syndrome in herniated lumbar disc was often not recognized early and a poor result of treatment was not rare, particularly in the patients who had acute onset and severe bladder dysfunction. So, early diagnosis and active surgical treatment are recommended for a better results of treatment.
Cauda Equina*
;
Diagnosis
;
Early Diagnosis
;
Female
;
Follow-Up Studies
;
Foot
;
Humans
;
Incidence
;
Leg
;
Low Back Pain
;
Male
;
Polyradiculopathy*
;
Prognosis
;
Retrospective Studies
;
Urinary Bladder
;
Urinary Retention
;
Urination