No abstract available.

No abstract available.
Bleomycin* ; Drug Therapy, Combination* ; Etoposide* ; Germ Cells* ; Neoplasms, Germ Cell and Embryonal*

No abstract available.
Bile Ducts, Extrahepatic* ; Carcinoma, Hepatocellular*

No abstract available.

Superficial siderosis (SS) of the central nervous system is a rare disease, which is caused by the accumulation of iron from the hemoglobin in the superficial layer of the brain, spinal cord, and central parts of cranial nerves. The etiology of SS is the accumulation of hemosiderin in the subarachnoid space due to chronic or repeated hemorrhage resulting in progressive and irreversible neurological dysfunction. The cause of the disease is aneurysm, trauma, tumor, and vascular malformation. In most cases, the cause of bleeding is unknown. Clinical features include sensorineural hearing loss, cerebellar ataxia, and myelopathy. Until now, magnetic resonance imaging (MRI) has only been diagnosed and there is no standardized treatment. We will investigate clinical features and MRI findings of SS disease in the central nervous system using 2 patient cases.
Aneurysm ; Brain ; Central Nervous System ; Cerebellar Ataxia ; Cranial Nerves ; Dizziness ; Hearing Loss, Sensorineural ; Hemorrhage ; Hemosiderin ; Humans ; Iron ; Magnetic Resonance Imaging ; Rare Diseases ; Siderosis ; Spinal Cord ; Spinal Cord Diseases ; Subarachnoid Space ; Vascular Malformations ; Vertigo

BACKGROUND: Osteoporosis of transplanted/grafted bone is well known in the immediate post bone transplantation/bone grafting period. In limb transplantation, the growth plates in the transplanted limbs retain their longitudinal growth properties. However, there is a paucity of reports on what happens to the bone and the growth potential of the growth plate when limb transplantation between a juvenile donor and an adult recipient is performed. MATERIALS AND METHODS: Ten juvenile to juvenile hind limb transplants and five juvenile to adult hind limb transplants were performed in male syngeneic Lewis rats. Osteoporosis in the isochronograft as well as the heterochronograft limbs was measured by 3D micro-CT. In addition, the increase in tibial length, after transplantation was measured and compared with the increase in the tibial length of the opposite non-operated limbs. RESULTS: The 3D CT parameters indicate a significantly inferior bone quality in the heterochronografts compared with the isochronografts. After transplantation, the increase in the tibial length of the isochronografts was similar the increase in length of the opposite juvenile non operated tibiae and the heterochronograft tibias. CONCLUSION: Age is a significant factor that affects the bone quality, resulting in post transplant osteoporosis in heterochronografts compared with isochronografts. However, the growth plate after transplantation remains unaffected by the difference in age and continues to grow at its own inherent rate in adult recipients as it does in the juvenile recipients.
Adult ; Animals* ; Extremities* ; Growth Plate* ; Humans ; Male* ; Osteoporosis ; Rats* ; Tibia ; Tissue Donors ; Transplantation ; Transplants

PURPOSE: Rat arthritis was induced by bovine type II collagen. To present the remedial effects on type II collagen arthritis and clinical efficacy, a high dose of methotrexate, of a level similar to that used in cancer patients was given to rats. MATERIALS AND METHODS: Healthy male Brown Norway rats were used, of mean weight; 300 g. There were 7 subgroups: a control group, given collagen only, an orally methotrexate treated (2.5mg/week), high methotrexate dose groups; 10 mg/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg and a low dose methotrexate group (0.375 mg/kg/week, 4 times) were included in the present study. Type II collagen was injected for arthritis induction and a single intraperitoneal injection of methotrexate was administered after 15 days. Orally treated group was administered 4 times (2.5 mg/week). Histopathologic findings were evaluated. RESULTS: 50 mg/kg methotrexate was effectively reduced the arthritis index and significantly decreased inflammatory cell number between 2nd and 6 weeks. CONCLUSION: A high dose of methotrexate may be clinically effective in the short period treatment of rheumatoid arthritis patients.
Animals ; Arthritis* ; Arthritis, Rheumatoid ; Cell Count ; Collagen ; Collagen Type II* ; Humans ; Injections, Intraperitoneal ; Male ; Methotrexate* ; Norway ; Rats*

Lacrimal fistula (LF) is a rare abnormality of the lacrimal system. Patients with LF are usually asymptomatic, and thus, treatment is unnecessary. During surgery on a patient with LF, the fistula may fall into the range of dissection. In such cases, fistula management becomes important. A 19-year-old woman visited our department to receive incisional blepharoplasty and medial epicanthoplasty, and a preoperative physical examination revealed LF. During surgery, we found the fistula tract to be within the dissection field, and thus, the LF was cut and cauterized. One year after the surgery, inflammation and hypertrophy of the remnant lacrimal duct occurred. The wound was stabilized by creating an opening that reconnected the stump and the overlying skin. Through this case, we hope to establish the appropriate strategy for managing LF detected during medial epicanthoplasty. As seen in our case, cauterization should be avoided because of the high recurrence rate of LF. Instead, as definitive treatment, fistulectomy should be performed, or the fistula should be moved along with the skin flap when a small skin flap is transferred.
Blepharoplasty ; Cautery ; Female ; Fistula* ; Hope ; Humans ; Hypertrophy ; Inflammation ; Lacrimal Apparatus ; Physical Examination ; Recurrence ; Skin ; Wounds and Injuries ; Young Adult

Lacrimal fistula (LF) is a rare abnormality of the lacrimal system. Patients with LF are usually asymptomatic, and thus, treatment is unnecessary. During surgery on a patient with LF, the fistula may fall into the range of dissection. In such cases, fistula management becomes important. A 19-year-old woman visited our department to receive incisional blepharoplasty and medial epicanthoplasty, and a preoperative physical examination revealed LF. During surgery, we found the fistula tract to be within the dissection field, and thus, the LF was cut and cauterized. One year after the surgery, inflammation and hypertrophy of the remnant lacrimal duct occurred. The wound was stabilized by creating an opening that reconnected the stump and the overlying skin. Through this case, we hope to establish the appropriate strategy for managing LF detected during medial epicanthoplasty. As seen in our case, cauterization should be avoided because of the high recurrence rate of LF. Instead, as definitive treatment, fistulectomy should be performed, or the fistula should be moved along with the skin flap when a small skin flap is transferred.
Blepharoplasty ; Cautery ; Female ; Fistula* ; Hope ; Humans ; Hypertrophy ; Inflammation ; Lacrimal Apparatus ; Physical Examination ; Recurrence ; Skin ; Wounds and Injuries ; Young Adult

PURPOSE: The purpose of this study is to investigate differentially expressed genes using DNA microarray between advanced gastric cancer (AGC) with aggressive lymph node (LN) metastasis and that with a more advanced tumor stage but without LN metastasis. MATERIALS AND METHODS: Five sample pairs of gastric cancer tissue and normal gastric mucosa were taken from three patients with T3N3 stage (highN) and two with T4N0 stage (lowN). Data from triplicate DNA microarray experiments were analyzed, and candidate genes were identified using a volcano plot that showed > or = 2-fold differential expression and were significant by Welch's t test (p < 0.05) between highN and lowN. Those selected genes were validated independently by reverse-transcriptase-polymerase chain reaction (RT-PCR) using five AGC patients, and tissue-microarray (TMA) comprising 47 AGC patients. RESULTS: CFTR, LAMC2, SERPINE2, F2R, MMP7, FN1, TIMP1, plasminogen activator inhibitor-1 (PAI-1), ITGB8, SDS, and TMPRSS4 were commonly up-regulated over 2-fold in highN. REG3A, CD24, ITLN1, and WBP5 were commonly down-regulated over 2-fold in lowN. Among these genes, overexpression of PAI-1 was validated by RT-PCR, and TMA showed 16.7% (7/42) PAI-1 expression in T3N3, but none (0/5) in T4N0 (p=0.393). CONCLUSION: DNA microarray analysis and validation by RT-PCR and TMA showed that overexpression of PAI-1 is related to aggressive LN metastasis in AGC.
Gastric Mucosa ; Humans ; Lymph Nodes* ; Neoplasm Metastasis* ; Oligonucleotide Array Sequence Analysis ; Plasminogen Activator Inhibitor 1 ; Plasminogen Activators* ; Plasminogen* ; Stomach Neoplasms*