1.Correlation of Expression of E-Cadherin, alpha-Catenin, beta-Catenin, and Clinicopathologic Parameters in Colorectal Adenocarcinomas.
Hyoung Joong KIM ; Tae Jin LEE ; Eon Sub PARK ; Jae Hyung YOO
Korean Journal of Pathology 2000;34(4):264-272
The E-cadherin, alpha-catenin, and beta-catenin expressions were immunohistochemically investigated in paraffin-embedded materials of 80 cases of colorectal adenocarcinomas. The staining similar to normal colorectal mucosa with preserved strong membranous staining pattern was considered normal or preserved expression. The X2 test was used to analyse the statistical correlation of cadherin/catenin expression with clinicopathologic parameters and the Breslow test for the correlation with survival length. Normal colorectal mucosa showed strong membranous expression of cadherin/catenin complex. The reduced E-cadherin, alpha-catenin, and beta-catenin expression were found in 53/80 (66.3%), 46/80 (57.5%), and 44/80 (55.5%) cases of colorectal cancers examined, respectively. There were significant correlations between E- cadherin and alpha -catenin (p=0.035), and between alpha-catenin and beta-catenin (p=0.013). The reduced E-cadherin expression was associated with histologic dedifferentiation, tumor depth, lymph node metastasis, clinical stage (p<0.05), poor clinical outcome in stage II (p=0.016) and the reduced alpha-catenin expression with lymph node metastasis and clinical stage (p<0.05). Reduced expression of two or more proteins was correlated with lymph node matastasis, histologic dedifferentiation, clinical stage, and survival (p<0.05). The present study demonstrates a significant down-regulation of E-cadherin and alpha-catenin expression in colorectal cancer is associated with tumor invasiveness, histologic dedifferentiation, lymph node metastasis, and clinical stage. These results suggest that E-cadherin and alpha-catenin may be useful markers of invasiveness, lymph node metastatic potential, and clinical stage and of value as prognostic markers in the earlier stage. Further studies are needed to confirm the prognostic value of these cadherin/catenin complex.
Adenocarcinoma*
;
alpha Catenin*
;
beta Catenin*
;
Cadherins*
;
Colorectal Neoplasms
;
Down-Regulation
;
Lymph Nodes
;
Mucous Membrane
;
Neoplasm Metastasis
2.Expression of Glutathione S-Transferase, E-Cadherin, and Catenins during N,N-Diethylnitrosamine-Induced Hepatocarcinogenesis in Rat Liver.
Hyoung Joong KIM ; Yon Sik YOO ; Tae Jin LEE ; Mi Kyung KIM ; Eon Sub PARK ; Jae Hyung YOO
Korean Journal of Pathology 2000;34(12):982-993
N,N-Diethylnitrosamine (DEN) has been proved to have carcinogenic potential in the initiation or promotion stage and the transformed cells proliferate to form preneoplastic nodules which are positive for placental form of glutathione S-transferase (GST-P). E-Cadherin, a member of the cadherin family, is expressed in epithelial cells. To evaluate the role of adhesion molecules (E-Cadherin, alpha-catenin, and beta-catenin), which have not been well understood in carcinogenesis, we investigated the changes of E-cadherin, alpha-Catenin and beta-Catenins by immunohistochemistry and immunoblotting in DEN-induced hepatocarcinogenesis of rat liver. In addition, the sequential analysis of histopathology and the expression of GST-P were also examined. Immunoreactive areas for GST-P were gradually increased from early period of carcinogenesis and strong GST-P positive foci were noted in various lesions, especially in the clear cell and eosinophilic cell nodules. Immunohistochemically, the E-Cadherin expression was increased in DEN-treated preneoplastic nodules in 4 and 10 weeks and hepatocellular carcinomas displayed relatively reduced expression compared with the hyperplastic nodules. But alpha- and beta-catenin expression was increased in hyperplastic nodules and hepatocellular carcinomas. Immunoblotting studies revealed that the level of alpha-catenin (cytosol and membranous fraction) was overexpressed in hyperplastic nodules as well as hepatocellular carcinomas, which showed markedly increased expression. The membranous fraction of beta-catenin was markedly increased in 10 weeks of DEN treatment and slightly reduced in hepatocellular carcinomas. These findings suggest that during DEN-induced hepatocarcinogenesis, the clear cell and eosinophilic cell nodules expressing GST-P in their cytoplasm are early transformed cell nodules. The altered expression of E-Cadherin and catenins is closely related with tumor propagation. Loss or reduced expression of E-cadherin may play a role in the progression of late hyperplastic nodule to hepatocellular carcinoma in DEN-induced rat hepato carcinogenesis.
alpha Catenin
;
Animals
;
beta Catenin
;
Cadherins*
;
Carcinogenesis
;
Carcinoma, Hepatocellular
;
Catenins*
;
Cytoplasm
;
Eosinophils
;
Epithelial Cells
;
Glutathione Transferase*
;
Glutathione*
;
Humans
;
Immunoblotting
;
Immunohistochemistry
;
Liver*
;
Rats*
3.Expression of MDM-2 and p53 Proteins in Gastric Adendegrees Carcinoma and Its Relationship with Clinicopathologic Factors.
Ji Woong YANG ; Hyoung Joong KIM ; Tae Jin LEE ; Eon Sub PARK ; Jae Hyoung YOO
Journal of the Korean Cancer Association 2000;32(3):476-486
PURPOSE: MDM-2 is an oncoprotein that inhibits p53 tumor-suppressor protein. These abnor malities have a role in tumorigenesis through inactivation of p53 function. To determine the clini copathological and prognostic value of MDM2 abnormalities in gastric adendegrees Carcinoma, MDM-2& p53 protein expression were analysed in surgically resected materials of gastric adendegrees Carcinoma. MATERIALS AND METHODS: Fifty cases which had got follow-up after surgical resection were immunohistdegrees Chemically studied with p53 and MDM-2 antibodies. We defined variable clinico pathologic factors for expression of p53 and MDM-2 protein and analysed their relationships. RESULTS: Immunohistdegrees Chemical stain revealed expression of MDM-2 protein as a 52.0% (26/50) and p53 protein 20.0% (10/50), respectively. But their expressions were not assdegrees Ciated with clinicopathological factors such as T-factor, N-factor, stage, histology and differentiation. Overall, p53-negative patients seemed to have a better prognosis regardless of MDM-2 protein status (P= 0.057). MDM-2 protein status was considered to have no play as a prognostic factor. CONCLUSION: In the gastric adendegrees Carcinoma, p53 protein expression seemed to have a inverse relationship with clinical outcomes but MDM-2 protein expression, which was observed more frequently than those of p53, seemed not to be prognostic indicator.
Antibodies
;
Carcinogenesis
;
Follow-Up Studies
;
Humans
;
Prognosis
;
Proto-Oncogene Proteins c-mdm2
;
Stomach Neoplasms
4.Transvaginal pulsed doppler assessment of luteal blood flow between normal and abnormal early pregnancy.
Jae Sook ROH ; Jung Bae YOO ; Moon Il PARK ; Kyung Tae KIM ; Yoon Young HWANG ; Hyoung MOON ; Jae Auk LEE
Korean Journal of Perinatology 1993;4(4):473-479
No abstract available.
Pregnancy*
5.A Case of Kerion Celsi Caused by Microsporum canis in Adult Man.
Seung Han LEE ; Woo Young SIM ; Mu Hyoung LEE ; Nack In KIM ; Choong Rim HAW ; Tae Yeon YOO
Korean Journal of Dermatology 1990;28(1):80-84
Kerion celsi is an inflammatory type of tinea capitis. We report a case of Kerion celsi caused by Micropsporum canis in 51 year-old man. When he visited our hospital, he suffered from hair loss and erythematous scaly patched and pustules on the scalp and papules on the face with tenderness and itching sensation. Cultures from scalp lesion and infected hair on Sabouraud glucose agar media showed Microsporum canis. Histopathologic findings showed graunlomatous changes in dermis and many spores around the hair shaft. Treatment was done topically with antifungal ointment and systernically with antibiotics, steroid and antifungsl agents. It is uncommon in adults, especially in men, but this condition must be considered when clinicians are evaluating patients with hair loss and nonspecific scalp dermatitis.
Adult*
;
Agar
;
Anti-Bacterial Agents
;
Dermatitis
;
Dermis
;
Glucose
;
Hair
;
Humans
;
Male
;
Microsporum*
;
Middle Aged
;
Pruritus
;
Scalp
;
Sensation
;
Spores
;
Tinea Capitis*
6.Changes of Bone Meneral Density of the Distraction Gap and Adjacent Parent Bone in Callotasis of the Cnanine Tibia
In Ho CHOI ; Kye Hyoung LEE ; Chin Youb CHUNG ; Tae Joon CHO ; Chung Hoon LEE ; Won Joon YOO ; Jung Joon YOO ; Duk Yong LEE
The Journal of the Korean Orthopaedic Association 1996;31(5):1080-1089
The purpose of this study are to delineate the temporal and spatial changes of bone mineralization at the distraction gap and adjacent parent bone, and to investigate the effect of microaxial dynamization on regenerate bone healing and on development of regional osteopenia at the adjacent parent bone, during callotasis. Sixteen Korean adult mongrel dogs underwent bilateral tibial lengthening by callotasis. To the right hindlimbs, no dynamization was applied and served as control group (group I, and axial elastic dynamization was conffered to the left hindlimbs (group II). Bone mineral density(BMD) was measured by dual X-ray absorptionmetry(DXA) at immediate post-operative day, post-operative 12 day, 22 day, 36 day, 50 day, 65 day, and 85 day. Quantitative computed tomogram(Q-CT) was also taken after sacrifice to analyze temporal changes of mineralization pattern in the distraction gap. The following results were obtained; 1. In both group I and II, the BMD was lowest at the interzone in the distraction gap and increased linearly toward the corticotomy surface. This pattern did not change with time until the consolidation of the distraction gap, but the difference of BMD between the interzone and adjacent parent bone decreased with progress of consolidation. 2. During the distraction period, BMD increased progressively at the distraction gap and adjacent parent bone in both group I and II. During consolidation period, although BMD of the distraction gap still increased progressively, that of the adjacent parent bone decreased progressively in group I; the more distant from the corticotomy surface, the more decreased the relative BMD. in group II, the decrease in BMD of the adjacent parent bone was less than that in group I which was statistically significant in mid-consolidation period. In conclusion, the new bone in the distraction gap during callotasis appeared to form in bilateral direction with linear gradient from interzone toward corticotomy surface. Dynamization during callotasis not only stimulated regenerate bone healing in the distraction gap, but also prevented the occurrence of transient regional osteopenia at the distant part of the adjacent bone during midconsolidation period.
Adult
;
Animals
;
Bone Density
;
Bone Diseases, Metabolic
;
Calcification, Physiologic
;
Dogs
;
Hindlimb
;
Humans
;
Miners
;
Osteogenesis, Distraction
;
Parents
;
Tibia
7.Eccrine Poroma of the Auricle: A Case Report.
Tae Hoon KONG ; Tae Hyoung HA ; Min Seob EOM ; Sang Yoo PARK
Korean Journal of Audiology 2014;18(3):151-152
Eccrine poroma is described as a benign neoplasm originating from the intraepidermal eccrine duct of sweat glands. This tumor is known to arise in bare skin areas, but more rarely appeared in head and neck region. A 54-year-old female presented with a mass on the retroauricular aspect of the left auricle. There was a soft, protruding, and purple-colored, solitary mass of about 1.0x1.0 cm in size. After authors performed an excisional biopsy, eccrine poroma was confirmed histopathologically. Thus, we report a rare case of eccrine poroma of the ear with the review of literature.
Biopsy
;
Ear
;
Ear Auricle
;
Female
;
Head
;
Humans
;
Middle Aged
;
Neck
;
Poroma*
;
Skin
;
Sweat Glands
8.The Effect of Education Program of Cadaver Dissection for the Paramedical Students.
Hyo Hyun YOO ; Chang Ho SONG ; Eui Hyeog HAN ; Hyoung Tae KIM
Korean Journal of Physical Anthropology 2014;27(3):145-154
A purpose of the study is to provide basic information in order to find better ways for more effective cadaver dissection education by analyzing education effect on paramedical students after cadaver dissection. By using questionnaires composed of 12 questions, we have surveyed 1,041 paramedical students who participate in cadaver dissection in 2013 and analysed the results. The paramedical students are the department of nursing science, operation treatment, physical therapy, alternative medicine, clinical pathology, emergency rescue and dental hygienics. As a result, we have found that cadaver dissection makes students have more respect for human life and dignity, helps students learn anatomy and major. Furthermore, the degrees of satisfaction, interest, intention of afterward participation in cadaver dissection afterward are high. However, the degrees of intention of cadaver donations are low. We need to develop cadaver dissection programme customized for each majors in order to helps students have more respect for human life and dignity, more professionalism, more effective cadaver dissection education.
Cadaver*
;
Clinical Medicine
;
Complementary Therapies
;
Education*
;
Emergencies
;
Humans
;
Intention
;
Nursing
;
Pathology
;
Surveys and Questionnaires
9.Production, Characterization, and Variable Region Analysis of Monoclonal Antibodies Specific for Hepatitis B Virus S Antigen.
Moo Young SONG ; Chang Seok KIM ; Sang Koo PARK ; Jae Sun LEE ; Tae Hyoung YOO ; In Young KO
Immune Network 2003;3(4):281-286
BACKGROUND: Hepatitis B virus (HBV) infection is one of the worldwide public health problem affecting about 300 million people. The envelope protein of HBV consists of three components known as preS1, preS2, and S antigen. According to the recent study, anti-HBs Ab showed effective neutralization ability against HBV from chronic hepatitis B and liver transplant patients, suggesting the possible development of therapeutic antibody. METHODS: Spleen cells immunized with S antigen of HBV were fused with myeloma cell line to obtain HBsAg specific monoclonal antibodies. High affinity antibodies against HBsAg (adr, ad and ay type) were selected by competitive ELISA method. Nucleotide sequence of the variable regions of monoclonal antibodies was analyzed by RT-PCR followed by conventional sequencing method. RESULTS: We produced 14 murine monoclonal antibodies which recognize S antigen of HBV. Two of them, A9-11 and C6-9 showed the highest affinity. The sequence analysis of A9-11 revealed that variable regions of the heavy chain and light chains are members of mouse heavy chain I (B) and light chain lambda 1, respectively. Likewise, the sequence analysis of C6-9 revealed that variable regions of the heavy chain and light chains are members of mouse heavy chain II (B) and light chain kappa 1, respectively. Neutralization assay showed that A9-11 and C6-9 effectively neutralize the HBV infection. CONCLUSION: These results suggest that A9-11 and C6-9 mouse monoclonal antibodies can be used for the development of therapeutic antibody for HBV infection.
Animals
;
Antibodies
;
Antibodies, Monoclonal*
;
Base Sequence
;
Cell Line
;
Enzyme-Linked Immunosorbent Assay
;
Hepatitis B Surface Antigens
;
Hepatitis B virus*
;
Hepatitis B*
;
Hepatitis B, Chronic
;
Hepatitis*
;
Humans
;
Liver
;
Mice
;
Public Health
;
Sequence Analysis
;
Spleen
10.Expression of p53, p21waf1/cip1, Cyclin D1 and Rb in Gastric Epithelial Proliferative Lesions.
Hyoung Joong KIM ; Tae Jin LEE ; Eon Sub PARK ; Jae Hyung YOO
Korean Journal of Pathology 2002;36(4):222-231
BACKGROUND: Aberrations of cell cycle-related genes have been reported to contribute to the formation and development of various human tumors. To investigate the gastric carcinogenesis, the expression of cell cycle-related genes (p53, p21wafl/cipl, cyclin D1 and Rb protein) compared to the morphological changes of gastric epithelial lesions were studied. METHODS: The expression of p53, p21wafl/cipl, cyclin D1 and Rb protein was immunohistochemically studied in a series of surgical specimens including the 36 normal/regenerating lesions and the 127 gastric epithelial proliferative lesions (GEPLs). The gastric epithelial proliferative lesions consisted of 25 regenerating epithelia with atypias (REAs), 27 low grade gastric dysplasias (LGDs), 17 high grade dysplasias (HGDs), 24 early gastrc carcinomas (EGCs), and 34 advanced gastric carcinomas (AGCs). RESULTS:The frequency of p53 protein overexpression was significantly associated with histologic grades of GEPLs (p=0.031); occurring in 4% of REAs, in 14.8% of LGDs, in 23.5% of HGDs, in 41.7% of EGCs and 58.9% of AGCs. The p21 wafl/cipl immunohistochemical reaction showed superficial eccentric positivity, representing an inverse correlation with histologic grades of GEPLs (p=0.04); occurring in 83.4% of normal/regenerating lesions, in 80% of REAs, in 74.1% of LGDs, in 29.4% of HGDs, 20.8% of EGCs and 8.8% of AGCs. Although Cyclin D1 and Rb proteins were expressed highly in the GEPLs, the frequency of both proteins were insignificantly associated with histologic grades of GEPLs (p=0.092). However, cases with both the Rb and cyclin D1 positivity were increased with statistical significance along histologic grades of GEPLs (p=0.044). CONCLUSIONS: The altered expression of p53, p21, Rb, and cyclin D1 was considered to be related to dysplastic progression and advancement of malignancy in GEPLs. Therefore, immunohistochemical studies of cell cycle related proteins and a combined analysis may be useful for estimating and following up cases of GEPLs.
Carcinogenesis
;
Cell Cycle
;
Cyclin D1*
;
Cyclins*
;
Humans
;
Retinoblastoma Protein