OBJECTIVE: The kinetic indices of apoptosis and cell proliferation m a histopathologic spectrum of the cervical neoplasia were evaluated to clarify the correlation between cervical neoplasm and apoplasis. Specific lesioas included cervical intraepithelial neoplasia(CIN), catcinoma in situ(CIS), and invasive carcinama. METHODS: Archival samples from normal cervical epithelium(n=7), low-grade squamous intraepithelial lesions(LGSIL, n=17), high-grade squamous intraepithelial lesions(HGSIL, n=17), invasive squamaus carcinoma(n=7) were evaluated for apoptosis and cell proliferation. Apoptotic cells were identified with terminal deoxynucleotidyl transferase-labeling of the 3'-OH end of DNA nucleosomes, and then apoptotic index(A.I sum of apoptotic bodies/ 1000 tumor cells) and total cell count(* 400 magnification) were calculated. RESULTS: In normal squamous epitheliam, Apoptotic bodies were mainly localized in ial layers, for low-grade squamous intraepithelial lesions(HGSIL) in superficial and intermediated layers, for high-pade squamous intraepithelial lesions(HGSIL) in intermediated and parabasal layers, for invasive carcinoma in full thic of squamous epithelium. Apototic indces(AI) in invasive carcinoma(mean: 6.21) were significantly higher than indices for high-grade squamous intraepithelial lesions(HGSIL, mean: 0.98) and high-grade squamous intraepithelial lesions(HGSIL, mean: 0.98) were significantly higher than indices for low-grade squamous intraepithelial lesions(LGSIL, mean: 0.12)(p<0.01), the total cell counts increased significantly as the specimens progressed toward invasive disease. (p<0.01) CONCLUSION: Apoptosis in cervical neoplasia appears to be closely related to poliferation and progression of the cervical squamous epithelial cell. This phenotype may allow identification of premalignant lesions with the potential to transform to cervical cancer.
Apoptosis* ; Cell Count ; Cell Proliferation ; DNA ; Epithelial Cells ; Epithelium ; Nucleosomes ; Phenotype ; Uterine Cervical Neoplasms

OBJECTIVE: Frozen-section evaluation of gynecologic tumors has historically been used to establish a histopatholgic diagnosis and to guide surgeons in appropriate surgical procedure. this study is to assess the clinical appropriation and accuracy of frozen-section biopsy in gynecologic tumors. METHODS: This is a retrospective quality assurance study all of frozen-section biopsy done at Chungnam National University Hospital during a three year period (1995 to 1997). There were 125 cases out of 2310 surgical specimens for gynecologic tumors, and the results were compared with final diagnoses based on fixed tissue specimens. RESULTS: Frozen section biopsy was accurate in 95.2% of all cases, in 96.3% (104/108) of ovarian tumor cases, and in 88.2% (15/17) of uterine tumor cases. Analysis of 6 inaccuracy cases revealed that most cases were sampling errors and occurred in mucinous tumor, belonging to all cases of ovarian tumors. Sensitivity, specificity, positive predictive value, and negative predictive value of frozen-section biopsy in ovarian tumors were 0.94, 1.00, 1, 00, 0.97. CONCLUSION: Frozen section biopsy is an appropriate method as a basic guideline during operations of gynecologic tumors and this method can obtains the highest accuracy when there is cooperation between experienced surgeons and reliable and careful pathologists. The accuracy of frozen section diagnosis has important implications regarding the type and extent of the primary surgery for gynecologic tumors.
Biopsy* ; Chungcheongnam-do ; Diagnosis* ; Frozen Sections* ; Mucins ; Retrospective Studies ; Selection Bias ; Sensitivity and Specificity

Our aim was to identify histologic changes in cervical carcinoma tumor cells due to chemotherapy,by electron microscopic examination. Cisplatin and 5-fluorouracil were used between March 1, 1994 and February 28, 1995 on a total of 16 patients. The treatment schedule consisted of 75 mg/m2 cisplatin via iv with hyperhydration on Day 1, in combination with 1000 mg/m2 5-fluorouracil via continuous iv on Day 1 to 5. The treatment was repeated every 4 weeks with a maxinum of 3 courses. Ultrastructurally, chemotherapy induced apoptosis,indicated by condensation of nucleus and cytoplasm,fragmentation of nuclei, and apoptotic bodies. Due to repeat chemotherapy, increased levels of desmosomes were noted and parts of tumor nests were replaced by mature squamous cells. Tumor nests were reduced in size, mitochondria exhibited swelling, the endoplasmic reticulum was dilated, and infiltration of inflammatory cells increased after chemotherapy. These results suggest that chemotherapy induces apoptosis and injury to the cytoplasm and nuclei of tumor cells. However, the exact selection mechanism of the biochemical pathway of cell death is not fuUy understood. Further study of the molecular biologic actions of cell death after chemotherapy is warranted.
Apoptosis ; Appointments and Schedules ; Cell Death ; Cisplatin ; Cytoplasm ; Desmosomes ; Drug Therapy* ; Endoplasmic Reticulum ; Fluorouracil ; Humans ; Mitochondrial Size

Intracranial lipoma usually occurs at or near the midline, predominantly in the callosal cistern and very rarely in the sylvian fissure. We report a case of lipoma of the sylvian fissure without association with other congenital anomaly or vascular abnormality.
Brain ; Lipoma*

OBJECTIVE: Tumor angiogenesis is essential for the progression and metastases of solid tumors. Microvessel density(MVD), a measure of tumor angiogenesis, has a prognostic significance in many types of tumors for predicting metastasis and survival. This study had two primary goals: 1. To determine how MVD correlates with tumor invasion in early squamous cell carcinoma(SCC) of the uterine cervix, and 2. To compare p53 protein expression in normal tissue with squamous cell lesions of the uterine cervix. METHODS: Quantification of MVD was performed on 59 specimens of cervical squamous cell lesions by immunohistochemical staining for factor VIII-related antigen. MVD was counted in a x200 field in the most active area of neovascularization. The same tumor sections were also immunohistochemically stains for p53 protein. RESULTS: 1. Compared with nonneoplastic areas, the degree of MVD was significantly increased in low- grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and squamous cell carcinomas(p=0.045). Microinvasive squamous cell carcinoma showed the highest MVD value. 2. p53 protein expression was increased in greater severity cases compared with mild cases(p=0.000). CONCLUSION: In the uterine cervix, MVD was significantly increased in microinvasive squamous cell carcinoma. p53 protein expression was well correlated with progression of squamous cell lesions.
Carcinoma, Squamous Cell ; Cervix Uteri* ; Coloring Agents ; Factor VIII ; Female ; Microvessels ; Neoplasm Metastasis ; von Willebrand Factor

PURPOSE: With the use of personal computers generalized, departmental society leveled computerization isgoing on in some other departments. So we tried to develop a program having a simple user interface, various retrieval functions and, analytic & statistic process system to effectively help patient care suitable for works concerned with department of diagnostic radiology and works of department. MATERIALS AND METHODS: This programdeals with such target works as department of diagnostic radiology and some works to need a lot of book keeping. Itis deviced to operate with windows (Microsoft, America), and central processing unit(486DX-2), memory unit(8Mbyte). As a developmental tool, Foxpro 2.6 for windows R(Microsoft, America). RESULTS: This Program can be easily accessed even by staffs poor at computer and it can make many books recording various check-ups and operations unnecessary, which were difficult to keep. Besides, it can keep data as a unified form, and so it provides patient care and other works with convenience and helps applying those stored data scientific research. CONCLUSION: The above result shows that works of department can be effectively controlled by analyzing or printing various check-up and operation done by department of diagnostic radiology.
Humans ; Memory ; Microcomputers* ; Patient Care

A 70-year old man presented with postprandial upper abdominal pain of two months duration, accompanied by indigestion, weight loss, and anorexia. There was no abnormality noted in the lab results. Abdominal CT showed a 3-cm round cystic mass in the tail of the pancreas. A distal pancreatectomy was done. The patient was discharged in 9 days. The cystic wall was composed of a keratinizing squamous epithelium surrounded by subepithelial, dense lymphoid tissue. Some clusters of the sebaceous gland were noted but there was no sweat gland or hair follicle. These findings were consistent with a lymphoepiethelial cyst with sebaceous differentiation. The patient was followed up for 12 months post operatively, and no recurrence was noted.
Abdominal Pain ; Aged ; Anorexia ; Dermoid Cyst* ; Dyspepsia ; Epithelium ; Hair Follicle ; Humans ; Lymphoid Tissue ; Pancreas* ; Pancreatectomy ; Pancreatic Neoplasms ; Recurrence ; Sebaceous Glands ; Sweat Glands ; Tomography, X-Ray Computed ; Weight Loss

A 70-year old man presented with postprandial upper abdominal pain of two months duration, accompanied by indigestion, weight loss, and anorexia. There was no abnormality noted in the lab results. Abdominal CT showed a 3-cm round cystic mass in the tail of the pancreas. A distal pancreatectomy was done. The patient was discharged in 9 days. The cystic wall was composed of a keratinizing squamous epithelium surrounded by subepithelial, dense lymphoid tissue. Some clusters of the sebaceous gland were noted but there was no sweat gland or hair follicle. These findings were consistent with a lymphoepiethelial cyst with sebaceous differentiation. The patient was followed up for 12 months post operatively, and no recurrence was noted.
Abdominal Pain ; Aged ; Anorexia ; Dermoid Cyst* ; Dyspepsia ; Epithelium ; Hair Follicle ; Humans ; Lymphoid Tissue ; Pancreas* ; Pancreatectomy ; Pancreatic Neoplasms ; Recurrence ; Sebaceous Glands ; Sweat Glands ; Tomography, X-Ray Computed ; Weight Loss

PURPOSE: To evaluate in reperfused myocardial infarction in a cat model, the time-course of signal enhancement as seen on Gadomer-17 enhanced MRI, and to correlate the size of the enhanced area with that of the infarct area as revealed by on histochemical examination. MATERIALS AND METHODS: Five cats which had undergone occlusion of the LAD followed by reperfusion underwent MR imaging. After T2-weighted imaging, Gadomer-17 enhanced T1-weighted images were obtained in four cats during a six-hour period, and in one during a three-hour period. Signal intensities were measured in the enhanced and non-enhanced areas of enhanced T1-weighted images. and using 2,3,5-triphenyl tetrazolium chloride (TTC) histochemical staining, the size of the abnormal signal area on each image was compared with that of the infarct area. RESULT: The enhanced area seen on enhanced T1-weighted images showed rapidly increased signal intensity following the administration of Gadomer-17. Maximum enhancement was detected during a 40 -60 minutes period, with an average enhancement of 168 +/-9.9% of normal myocardium. TTC staining revealed that the size of the high signal area on T2-weighted images and of the enhanced area on enhanced T1-weighted images was greater than that of the infarct area (p<0.05). CONCLUSION: In reperfused myocardial infarction in a cat model, Gadomer-17 enhanced MR imaging delineates both reversibly and irreversibly damaged myocardium, with strong enhancement and a broad temporal window. We may therefore expect that Gadomer-17 is useful for demonstrating myocardial injury.
Animals ; Cats* ; Magnetic Resonance Imaging* ; Myocardial Infarction* ; Myocardium ; Reperfusion

Metastasis to the skin occurs rarely in gynecologic cancer. Although carcinoma of the cervix is the fifth most common malignancy in Korean women, cutaneous involvement originating from cervical cancer is unusual. Common pattern of occurrence is multiple nodule in abdomen, vulva, lower extremities. We report a case of metastasis from squamous cell carcinoma of cervix to vulva. The patient was diagnosed with cervical cancer IIA. The extensive skin lesion on the vulva occurred 3 months after neoadjuvant chemotherapy, radical hysterectomy and concurrent chemoradiation. We have experienced such a case and report the case with brief review of literatures.
Abdomen ; Carcinoma, Squamous Cell ; Cervix Uteri ; Female ; Humans ; Hysterectomy ; Lower Extremity ; Neoplasm Metastasis ; Skin ; Uterine Cervical Neoplasms ; Vulva