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Tumor-draining lymph node (TDLN) lymphocytes contain immunologically sensitized to tumor but functionally deficient T cells. The 30 kDa protein antigen, a major secreted protein antigen of Mycobacterium tuberculosis, exhibits strong T cell stimulatory effect. In this study, it examined that the feasibility of using M tuberculosis 30 kDa antigen to stimulate tumor-draining lymph node cells for the generation of specific immune effector cells. Freshly isolated TDLN lymphocytes could directly respond to the 30 kDa antigen alone and their proliferative responses were markedly augmented by stimulation with rIL-2. TDLN cells were stimulated with the 30 kDa antigen for various time intervals and examined for the induction of IFN-r and IL-4 mRNA using RT-PCR. The expression of IFN-r mRNA was greatly augmented after 1 wk, whereas IL-4 mRNA is markedly decreased after 1 wk. Cytotoxic T cell activities induced by the 30 kDa antigen was also evaluated. TDLN cells stimulated with the 30 kDa antigen alone were able to generate remarkable cytotoxic response to K562 or Daudi cell lines after 6 days of culture. And their cytotoxic effects were highly augmented by stirnulation with rIL-2. These results suggest that the 30 kDa antigen of M. tuberculosis may selectively activate Thl cells of TDLN lymhocytes and induce the cytotoxic T cell activities. In conclusion, the 30 kDa antigen can be used as a biologic response modifier in tumor immunology.
Allergy and Immunology ; Cell Line ; Interleukin-4 ; Lymph Nodes* ; Lymphocytes* ; Mycobacterium tuberculosis* ; Mycobacterium* ; RNA, Messenger ; T-Lymphocytes ; Th1 Cells* ; Tuberculosis

10.Two Cases of Lichen Amyloidosis.

Jung Bock LEE ; Wook Hwa BARK ; Ryo Sung KIM ; Tae Ha WOO

Korean Journal of Dermatology 1970;8(2):51-54

Lichen amyloidosis is a chronic, benign and relatively rare disease, but not infrequent in Korea. The patients with lichen amyloidosis visited to Dermatologic Department of Severance Hospital in 1969. The results of clinico-pathological findings observed in above patients were presented. Case 1-A 56 year old farmer has been suffered from pruritic lichenoid papules which developed in the right pretibial area for 10 years. These papules were reddish-brown in color, 1 to 2mm in. diameter and closely aggregated. The whole gross lesion measured 10 by 25 cm. Cases 2-A 32 year old obese house-wife complained of pruritic eruptions in the right pretibial area for 3 years. Shiny firm papules, measuring approximately 2 mm in diameter, were red in color and gross entire lesion was estimated to 7 by 10 cm. Histological examination by employing the stain method of crystal violet and the Nomland test demonstrated the amyloid mass in the papillary portion of dermis. All laboratory studies including complete blood count (CBC), chest X-ray, sedimentation rate, urinalysis (including test for Bence Jones protein), blood glucose, blood urea nitrogen (BUN), serum cholesterol, venereal disease research laboratories test for syphilis (VDRL), stool for occult blood, serum protein electrophoresis, electrocardiogram (ECG) and intravenous congo red test, were within normal limit. The therapeutic results were not promissing, however itching and lichenoid papules were disappeared during the treatment with steroid cream for occlussive dressing therapy.
Adult ; Amyloid ; Amyloidosis* ; Bandages ; Blood Cell Count ; Blood Glucose ; Blood Urea Nitrogen ; Cholesterol ; Congo Red ; Dermis ; Electrocardiography ; Electrophoresis ; Gentian Violet ; Humans ; Korea ; Lichens* ; Middle Aged ; Occult Blood ; Pruritus ; Rare Diseases ; Sexually Transmitted Diseases ; Syphilis ; Thorax ; Urinalysis

1.A clinical analysis of the appendicitis in children.

2.Purification and immunochemical charaterization of alpha-antigen from the culture filtrate of mycobacterium tuberculosis.

3.Determination of antibody activities of alpha- and beta-protein antigens of mycobacterium tuberculosis in cerebrospinal fluid by ELISA for the diagnosis of tuberculous meningitis.

4.Biological detection of enterotoxigenic E. coli.

5.Detection of mycobacterium tuberculosis in sputum samples by polymerase chain reaction.

6.Purification of 30-kDa and 32 kDa protein antigens from mycobacterium tuberculosis and activation of human monocytes by lymphokines.

7.Lymphocyte proliferation and antibody response against 30-kDa protein antigen of mycobacterium tuberculosis.

8.Successful coil embolization of ruptured hepatic artery pseudoaneurysm.

9.In vitro Stimulation of Tumor - Draining Lymph Node Lymphocytes with the 30 kDa Antigen of Mycobacterium tuberculosis Leads to the Differentiation of Th1 Cells and Cytotoxic Effector Cells.

10.Two Cases of Lichen Amyloidosis.

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