Search Results

Some of the proteins of mycobacteria are preferentially associated with the cell wall and are powerful immunogens, and humoral antibody responses to these mycobacterial antigens may occur in patients with tuberculosis. In this study, Triton X-100 solubilized protein (TSP) antigen was isolated from Mycobacterium tuberculosis H37Rv by overnight shaking with 1% Triton X- 100/PMSF and 10-90% ammonium sulfate precipitation. IgG and IgM antibody levels against TSP, crude protein from the unheated cultrue filtrate (CF#) and 30 kDa antigens were determined in the sera of 80 patients with pulmonary tuberculosis and 99 healthy controls with PPD (+) and (-). High IgG reactivity to TSP and CF antigen was observed in tuberculosis patients. Mean IgG antibody titers against all of three mycobacterial antigens were differed significantly (P<0.01) between patients and controls but IgM showed no difference. By the cut-off value adding 2 standard deviation to the mean absorbance of controls, the sensitivity and specificity of the IgG antibody to TSP antigen were 93.9% and 77.5%. The specificity to TSP antigen was a litttle higher than those obtained by CF and 30 kDa antigen. From the above results, the TSP antigen may be useful for the serodiagnosis of tuberculosis.
Ammonium Sulfate ; Antibody Formation ; Cell Wall ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Mycobacterium tuberculosis* ; Mycobacterium* ; Neptune* ; Octoxynol* ; Sensitivity and Specificity ; Serologic Tests ; Tuberculosis ; Tuberculosis, Pulmonary*

10.Three Cases of Iridoeorneal Endothelial Syndrome.

Tae Min BAEK ; Jang Ho KIM ; Jung Eon YONG ; Joo Hwa LEE

Journal of the Korean Ophthalmological Society 1991;32(9):747-751

1.A clinical analysis of the appendicitis in children.

2.Biological detection of enterotoxigenic E. coli.

3.Purification and immunochemical charaterization of alpha-antigen from the culture filtrate of mycobacterium tuberculosis.

4.Determination of antibody activities of alpha- and beta-protein antigens of mycobacterium tuberculosis in cerebrospinal fluid by ELISA for the diagnosis of tuberculous meningitis.

5.Lymphocyte proliferation and antibody response against 30-kDa protein antigen of mycobacterium tuberculosis.

6.Detection of mycobacterium tuberculosis in sputum samples by polymerase chain reaction.

7.Purification of 30-kDa and 32 kDa protein antigens from mycobacterium tuberculosis and activation of human monocytes by lymphokines.

8.Successful coil embolization of ruptured hepatic artery pseudoaneurysm.

9.Determination of Anti-tuberculous Antibody Against Triton X-100 Solubilized Protein ( TSP ) Antigen of Mycobacterium tuberculosis in the Sera of Patients with Pulmonary Tuberculosis.

10.Three Cases of Iridoeorneal Endothelial Syndrome.

Display Mode

Output Records

File Type