1.Molecular Typing of the Methicillin-Resistant Determinant (mec) and Coagulase Typing as Epidemiologic Markers for Study of Nosocomial Infections Caused by Methicillin-Resistant Staphylococcus aureus.
Jung Man KIM ; A Seong KIM ; Kyeong Hee KIM ; Tae Gyeom KIM ; Jin Yeong HAN ; In Hoo KIM
Korean Journal of Clinical Pathology 1997;17(4):588-597
BACKGROUND: Methicillin-resistant Staphylococcus aureus(MRSA) is an increasingly common cause of nosocomial infections worldwide. Epidemiologic investigation of MRSA outbreaks and identification of pathways of nosocomial MRSA spread require the ability to distinguish individual MRSA strains. We applied molecular tap ing of the methicillin-resistant determinant (mec) and coagulase typing in the investigation of a nosocomial MRSA infections. METHODS: We randomly selected 79 strains of mecA positive MRSA isolated from patients who visited Dong-A university Hospital from Dec. 1995 to Oct. 1996. Molecular typing of MRSA was performed by comparing the size of the mac-associated hypervariable region amplified by the polymerase chain reaction (PCR). Coagulase typing with type I-VIII antisera was also used for classification of MRSA based on its phenotype. Each isolates were classified by the combination of molecular analyses and coagulase type. RESULTS: The 79 MRSA isolates were grouped Into sin hypervariable legion (HVR) genotypes on the basis of the size of the PGR products. In coagulase typing, the most predominant type was II(46.8%) and type V was not found. Nine strains were not typable. The combination of HVR genotypes and coagulase types showed 23 different types in 79 MRSA Isolates. The strains which were repeatedly isolated from the same patients showed the same HYR genotypes and coagulate types. CONCLUSION: The combination of HVR genotypes and coagulase types is thought to be useful in epidemiolgical Investigation of nosocomial infections caused by MRSA ,because of its simplicity and reproducibility.
Classification
;
Coagulase*
;
Cross Infection*
;
Disease Outbreaks
;
Genotype
;
Humans
;
Immune Sera
;
Methicillin Resistance*
;
Methicillin-Resistant Staphylococcus aureus*
;
Molecular Typing*
;
Phenotype
;
Polymerase Chain Reaction
;
Staphylococcus
2.Identification of an i(21q) by Using Dinucleotide Repeat Polymorphisms.
Kyeong Hee KIM ; Tae Gyeom KIM ; Jin Yeong HAN ; Jung Man KIM ; Joo In PARK ; In Hoo KIM
Korean Journal of Clinical Pathology 1997;17(1):183-189
BACKGROUND: Recent DNA polymorphism analysis using numerous DNA markers has been used to determine the parental origin of the extra chromosome 21 in Down syndrome. In this study we used seven dinucleotide repeat polymorphisms on chromosome 21 to characterize a case of rea(21q21q) and to know whether it is consistent with an isochromosome or a true Robertsonian translocation. METHODS: Cytogenetic investigation was done by conventional G banding DNA was extracted from whole blood of a proband and her parents and was amplified by PCR using seven sets of (GT)n repeat dinucleotide markers located on the long arm of chromosome 21 After electrophoresis of the PCR product in polyacrylamide gel and silver staining the parental origin and number of DNA copy were determined by visual comparison of the band intensities within and between individuals. RESULTS: Conventional cytogenetics showed that the proband had a 46.XX.re(21q21q) chromosome pattern. Parental chromosome studies were normal, therefore, the rearrangement was a de novo event. All seven DNA markers showed one or two alleles, demonstrating rea(21q21q) to be an isochromosome. For D21S215 and D21S156 markers both parents were heterozygous and the proband inherited one copy of paternal allele and two copies of maternal allele which both parents did not share. This finding was consistent with a maternally derided isochromosome. CONCLUSION: Use of dinucleotide repeat DNA polymorphisms after PCR amplification will be very useful to detect the parental origin of additional chromosome 21 or rearrangement of chromosome 21 in Down syndrome. Besides employing siltier staining of a PCR product we will be able to avoid using of radioisotopes and apply to clinical laboratory diagnosis.
Alleles
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Arm
;
Chromosomes, Human, Pair 21
;
Clinical Laboratory Techniques
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Cytogenetics
;
Dinucleotide Repeats*
;
DNA
;
Down Syndrome
;
Electrophoresis
;
Genetic Markers
;
Humans
;
Isochromosomes
;
Parents
;
Polymerase Chain Reaction
;
Radioisotopes
;
Silver Staining
3.Detection of the Pallister-Killian Syndrome by G-Banding and FISH in Cultured Skin Fibroblasts.
Jin Yeong HAN ; Tae Gyeom KIM ; Lisa G SHAFFER
Korean Journal of Clinical Pathology 1998;18(2):284-287
Pallister-Killian syndrome is a rare disorder characterized by multiple congenital anomalies, coarse face, profound mental retardation, and epilepsy. Chromosomes of peripheral lymphocytes are usually normal, however, tissue cultures show varying degree of mosaicism for an extra metacentric chromosome i(12)(p10). We report on a two-year-old boy with Pallister-Killian syndrome confirmed by FISH in cultured skin fibroblasts. The patient had myoclonic seizures beginning at 2 months and was delayed in physical and speech development. Craniofacial manifestations include sparsity of scalp hair, hypertelorism, sparse eyebrows, flat nasal bridge, and large ears. Cytogenetic analysis of peripheral lymphocytes done at another hospital was reported to be normal. Studies of his skin fibroblasts showed an extra small metacentric i(12p) chromosome in 100% of metaphases. FISH using of whole chromosome painting probe for chromosome 12 confirmed that the supernumerary chromosome was an isochromosome 12p.
Chromosome Painting
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Chromosomes, Human, Pair 12
;
Cytogenetic Analysis
;
Ear
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Epilepsy
;
Eyebrows
;
Fibroblasts*
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Hair
;
Humans
;
Hypertelorism
;
Intellectual Disability
;
Isochromosomes
;
Lymphocytes
;
Male
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Metaphase
;
Mosaicism
;
Scalp
;
Seizures
;
Skin*
4.Molecular Epidemiological Typing of Clinical Strains of Methicillin-Resistant Staphylococcus aureus.
Jung Man KIM ; Kyeong Hee KIM ; Tae Gyeom KIM ; Young Gil LEE ; Kyeong HEO ; Yoo Jung SONG ; In Hoo KIM
Korean Journal of Clinical Microbiology 1999;2(1):40-48
BACKGROUND: Meicillin-resistant Staphylococcus aureus(MRSA) is a common cause of nosocomial infections worldwide. Identification of strains by molecular typing facilitates epidemiological studies and improves disease control This study was performed to determine the usefulness of mecA-associated hypervariable region(HVR) polymerase chain reaction (PCR) and random amplified polymorphic DNA(RAPD) analysis in the investigation of a nosocomial MRSA infections. METHODS: Methicillin-resistance was identified by NCCLS disk diffusion method using the oxacillin disk. And PCR was done for detection of mecA gene. Antimicrobial susceptibility test, HVR-PCR and RAPD using 3 primers were performed for epidemiological analysis on isolates of MRSA. RESULTS: During the period from 1997 Dec. to 1998 May, 120 strains of S. aureus were isolated from clinical specimens. Among them, 78 strains were MRSA, and 72 strains were mecA positive. The strains of mecA positive MRSA were classified into four types by antibiogram, six genotypes by HVR-PCR, and 29 groups by RAPD using three primers. The combination of HVR genotypes and RAPD analysis showed 43 different types in 72 mecA positive MRSA isolates The five strains which were repeatedly isolated from the same patients showed the same HVR genotypes and RAPD analysis. CONCLUSIONS: Antibiogram, HVR-PCR, and RAPD could classify MRSA isolates into only 4-6 types, respectively, but combination of these methods could improve the typability. And combination of results of RAPD analysis using three primers were better than that using one primer in epidemiological studies of MRSA because of same reasons. It can be concluded that molecular typing of MRSA using HVR-PCR and RAPD assay is useful in epidemiolgical investigation of nosocomial infections caused by MRSA, because of its simplicity and reproducibility.
Cross Infection
;
Diffusion
;
Epidemiologic Studies
;
Genotype
;
Humans
;
Methicillin Resistance*
;
Methicillin-Resistant Staphylococcus aureus*
;
Microbial Sensitivity Tests
;
Molecular Typing
;
Oxacillin
;
Polymerase Chain Reaction
;
Staphylococcus
5.Role of Gamma Globin Promoter Region -269~-240 in Hydroxyurea Treated Erythroid Progenitor Cells.
Joo In PARK ; Tae Gyeom KIM ; Deok In KIM ; In Hoo KIM ; Jin Sook JEONG ; Jin Yeong HAN
Korean Journal of Clinical Pathology 1998;18(1):29-34
BACKGROUND: Recently, a great deal of interest has been focused on the use of hydroxyurea and hemin that may augment Hb F levels in patients with hemoglobinopathies and thalassemia, although the molecular mechanism of those chemicals remains unclear. In this study, we examined the effects of hydroxyurea and hemin on human adult peripheral and cord blood erythroid cells grown in a two-phase liquid culture system. METHODS: Four adult peripheral and four cord blood cells were cultured in two-phase liquid culture, and were treated with hydroxyurea or hemin. We counted isolated erythroid cells by acid benzidine and glycophorin A stains. To determine whether transcription factor binding to the promoter is critical, we also examined the promoter region of gamma globin gene both under uninduced and hydroxyurea or hemin induced conditions using gel mobility shift assay and southwestern blot analysis. RESULTS: When added together with erythropoietin, hydroxyurea led to significant increase in the percentage of erythroid cells in cord blood. In contrast, hemin greatly accelerated hemoglobin accumulation in adult erythroid progenitor cells. At -230 and -264 regions of gamma globin gene promoter, different protein binding patterns were observed in uninduced and hydroxyurea or hemin induced conditions between adult and cord blood. CONCLUSIONS: These results suggest that hydroxyurea and hemin may act via alteration in DNA-protein interactions to induce gamma globin gene expression. In addition, we can conclude that different transcription factors may be involved in the gamma globin induction process between the adult and cord blood erythroid cells.
Adult
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Blotting, Southwestern
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Coloring Agents
;
Electrophoretic Mobility Shift Assay
;
Erythroid Cells
;
Erythroid Precursor Cells*
;
Erythropoietin
;
Fetal Blood
;
gamma-Globins*
;
Gene Expression
;
Glycophorin
;
Hemin
;
Hemoglobinopathies
;
Humans
;
Hydroxyurea*
;
Promoter Regions, Genetic*
;
Protein Binding
;
Thalassemia
;
Transcription Factors
6.Effects of Ginseng Radix on the Cell Cycle Regulation in Human Fetal Osteoblast.
Dae Gyeom KIM ; Yong Bae LEE ; Sang Kee PARK ; Hyung Keun YOU ; Kyung Tae YOU ; Yun Chul KIM ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 2003;33(3):415-437
Ginseng Radix(GR) had been used widely from oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The results were as follows. Increased cell proliferation was observed in cells exposed to 100 ng/ml, 10 ng/ml of GR-1 at 12 hours and 24 hours, 1 microgram/ml of GR-1 at 48 hours, and 100 microgram/ml, 10 microgram/ml of GR-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, with 100 microgram/ml and 10 microgram/ml of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4 and CDK 6 were increased in the group of treated with 1 microgram/ml and 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, with 100 microgram/ml and 10 microgram/ml of GR-3. On the other hand, p21 was decreased in the treatment group with 1 microgram/ml and 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, 10 microgram/ml of GR-3, and p53 and p16 was decreased in the treatment group with 100 ng/ml of GR-1, 100 microgram/ml GR-3 and pRb was decreased in the all treatment groups except 1 microgram/ml of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4, CDK 6 and decreased cell cycle regulation protein levels of p21, pRb.
Humans
7.Measuring Trends in the Socioeconomic Burden of Disease in Korea, 2007-2015
Tae Eung KIM ; Ru-Gyeom LEE ; So-Youn PARK ; In-Hwan OH
Journal of Preventive Medicine and Public Health 2022;55(1):19-27
This study estimated the direct and indirect socioeconomic costs of 238 diseases and 22 injuries from a social perspective in Korea from 2007 to 2015. The socioeconomic cost of each disease group was calculated based on the Korean Standard Disease Classification System. Direct costs were estimated using health insurance claims data provided by the National Health Insurance Service. The numbers of outpatients and inpatients with the main diagnostic codes for each disease were selected as a proxy indicator for estimating patients’ medical use behavior by disease. The economic burden of disease from 2007 to 2015 showed an approximately 20% increase in total costs. From 2007 to 2015, communicable diseases (including infectious, maternal, pediatric, and nutritional diseases) accounted for 8.9-12.2% of the socioeconomic burden, while non-infectious diseases accounted for 65.7-70.7% and injuries accounted for 19.1-22.8%. The top 5 diseases in terms of the socioeconomic burden were self-harm (which took the top spot for 8 years), followed by cirrhosis of the liver, liver cancer, ischemic heart disease, and upper respiratory infections in 2007. Since 2010, the economic burden of conditions such as low back pain, falls, and acute bronchitis has been included in this ranking. This study expanded the scope of calculating the burden of disease at the national level by calculating the burden of disease in Koreans by gender and disease. These findings can be used as indicators of health equality and as useful data for establishing community-centered (or customized) health promotion policies, projects, and national health policy goals.
8.Cytogenetical, Morphological, and Immunophenotypical Characteristics of Myeloid Malignancies with t (8;21).
Kyeong Hee KIM ; Tae Gyeom KIM ; Jin Yeong HAN ; Jung Man KIM ; Jae Seok KIM ; Hyo Jin KIM ; Young Ho LEE ; Eun Yup LEE
Korean Journal of Hematology 1999;34(1):18-26
BACKGROUND: Myeloid malignancies carrying t (8;21) (q22;q22) exhibit several characteristic features. This translocation is most often associated with acute myeloid leukemia with maturation (AML-M2), but rarely with myelodysplastic syndrome. We studied the correlation of cytogenetics, morphology, and immunophenotype in 21 myeloid malignancies carrying t (8;21). METHODS: We analyzed 21 t (8;21) positive myeloid malignancies for morphology, immunophenotype and cytogenetics, retrospectively. We also performed reverse transcription polymerase chain reaction (RT-PCR) for AML1-ETO fusion transcripts using marrow slides stored at room temperature. RESULTS: 17 patients were diagnosed as French-American-British (FAB) type M2 and 4 cases as refractory anemia with excess blasts in transformation (RAEB-t). Three patients had marrow eosinophilia and 14 cases (67%) had Auer rods in leukemic blasts. Three patients (14%) developed granulocytic sarcomas. Four patients had variant t (8;21) translocation and 16 (76%) had secondary clonal abnormalities, most commonly loss of a sex chromosome (52%). 15 patients (83%) aberrantly expressed CD19. AML1-ETO fusion transcript of same size was observed in classic and variant t (8;21) translocation (11/14, 79%). CONCLUSION: Our study showed that myeloid malignancies carrying t (8;21) have distinctive morphological, immunophenotypical, and molecular features. These results can be aid to understand the pathogenesis and stratify treatment of those malignancies in the future.
Anemia, Refractory
;
Bone Marrow
;
Cytogenetics
;
Eosinophilia
;
Humans
;
Leukemia, Myeloid, Acute
;
Myelodysplastic Syndromes
;
Polymerase Chain Reaction
;
Retrospective Studies
;
Reverse Transcription
;
Sarcoma, Myeloid
;
Sex Chromosomes
9.Quantitative Analysis of Cultured Erythroid Progenitors in Liquid System.
Jin Yeong HAN ; Tae Gyeom KIM ; Kyeong Hee KIM ; A Seong KIM ; Hae Rahn BAE ; Duk Joon SUH ; Seol Mi KANG ; Joo In PARK ; In Hoo KIM
Korean Journal of Hematology 1997;32(1):32-40
BACKGROUND: Hemopoiesis and erythropoiesis have been studied mainly in immortalized cell lines and semisolid medium. But cell lines do not represent normal erythropoiesis, besides, in semisolid medium the cells are immobilized that it is difficult to do additional immunologic, biochemical, and molecular biologic experiments. In the present study we used a two-phase liquid culture system to isolate and quantify erythroid progenitors from peripheral blood and cord blood. METHODS: Peripheral and cord blood were obtained from three healthy donors and three full-term deliveries, respectively. Mononuclear cells were separated by density gradient centrifugation and were cultured in the first phase at a density of 5x106/mL in alpha- minimal essential medium ( -MEM). After 5~7 days of incubation at 37degrees C in an atmosphere of 5% CO2 with extra humidity, the nonadherent cells were harvested and recultured in the original volume of -MEM containing 10ng/mL stem cell factor and 1U/mL erythropoietin (EPO). Cellular morphology was observed by preparing cytocentrifuge slides stained with Wright Giemsa. On days 8, 10, 12, and 16 of the second phase, hemoglobin (Hb)- containing cells were counted on hemocytometer after staining with acid benzidine and glycophorin A-positive erythroid cells were scored by a flow cytometer. RESULTS: Pronormoblasts first started to appear on days 4~5 in the secondary culture. On day 10 basophilic normoblasts could be seen and on days 12~14 orthochromatic normoblasts were present. Both from peripheral and cord blood the maximum number of benzidine and glycophorin A-positive cells were achieved after 10 days and the total erythroid cell yield was approximately 1x106/mL. CONCLUSION: Using two-phase liquid culture, erythroid cell yield reached 1x106/mL both from peripheral and cord blood. In addition, this culture system permits the study of the effect of various culture conditions and components without terminating the culture, therefore it might provide us a useful experimental tool for studying pathogenesis and therapeutic modalities in genetic erythroid disorders as well as erythroid cell development.
Atmosphere
;
Basophils
;
Cell Line
;
Centrifugation, Density Gradient
;
Erythroblasts
;
Erythroid Cells
;
Erythropoiesis
;
Erythropoietin
;
Fetal Blood
;
Glycophorin
;
Humans
;
Humidity
;
Stem Cell Factor
;
Tissue Donors
10.Comparative Analysis of the Physical and Biochemical Properties of Light-cure Resin-modified Pulp Capping Materials
Tae Gyeom KIM ; Jongsoo KIM ; Joonhaeng LEE ; Jisun SHIN ; Mi Ran HAN ; Jongbin KIM ; Yujin KIM ; Jae Hee PARK
Journal of Korean Academy of Pediatric Dentistry 2024;51(2):149-164
This study compared the solubility, water absorption, dimensional stability, release of various ions (hydroxyl, calcium, sulfur, strontium, and silicon), and cytotoxicity of lightcured resin-modified pulp-capping materials. Resin-modified calcium hydroxide (Ultrablend™ plus, UBP), light-cured resin-modified calcium silicate (TheraCal LC™, TLC), and dual-cure resin-modified calcium silicate (TheraCal PT™, TPT) were used. Each material was polymerized; solubility, 24-hour water absorption, and 30- day dimensional stability experiments were conducted to test its physical properties. Solubility was assessed according to the ISO 6876 standard, and 24 hours of water absorption, 30 days of dimensional stability were assessed by referring to the previous protocol respectively. Eluates at 3 and 24 hours and on 7, 14, and 28 days were analyzed according to the ISO 10993-12 standard. And the pH, Ion-releasing ability, cell proliferation rate, and cell viability were assessed using the eluates to evaluate biochemical characteristics. pH was measured with a pH meter and Ion-releasing ability was assessed using inductively coupled plasma atomic emission spectrometry (ICP-AES). Cell proliferation rate and cell viability were assessed using human dental pulp cells (hDPCs). The former was assessed by an absorbance assay using the CCK-8 solution, and the latter was assessed by Live and Dead staining. TPT exhibited lower solubility and water absorption than TLC. UBP and TPT demonstrated higher stability than TLC. The release of sulfur, strontium, calcium, and hydroxyl ions was higher for TLC and TPT than for UBP. The 28-day release of hydroxyl and silicon ions was similar for TLC and TPT. TLC alone exhibited a lower cell proliferation rate compared to the control group at a dilution ratio of 1 : 2 in cell proliferation and dead cells from Live and Dead assay evaluation. Thus, when using light-cure resin-modified pulp-capping materials, calcium silicate-based materials can be considered alternatives to calcium hydroxide-based materials. Moreover, when comparing physical and biochemical properties, TPT could be prioritized over TLC as the first choice.