The residue levels of three kinds of organochlorines, BHC, DDT and PCB, in the serum were determined in farmers (40-59 years old, n=211), non-farmars (40-59 years old, n =27) and high school students (15-18 years old, n=56) to clarify the factors that contributed to the accumulation of those three compounds in the blood of farmers and rural inhabitants. The average values of those compounds were found to be 9.1±5.0 ppb for B-BHC, 9.2±6.3 ppb for pp'-DDE and 7.1 ±5.7 ppb for PCB. There was marked difference in the value between districts in which the subjects live. The subjects could be divided into the lower concentration group and higher concentration group, showing high discrimination ratio (72-83%) by Hayashi's quantification theory II. Statistical analysis showed that the factors contributing to the accumnlation of those compounds in the present subjects are related with digestion of chicken and fish, Hb concentration, serum cholinesterase activity, liver function and serum trigliceride and cholesterol levels, and use of organochlorines and other pesticides, duration and the mumber of days of pesticide spraying, experience of pesticide poisoing, cultivation of specific crops. Those results, in greater part, supported the general recognition that organochlorine residues in human body might be regulated by changning eating patterns and avoiding those food items containing those chemical compounds. On the other hand, attention should be focused on the exposure of those compounds through the use of specific pesticides including those compounds. And further investigation is necessary for clarifying the contributing factors to the accumulation of those compounds in human body especially in the higher residue group discriminated in the present study.

A questionnaire survey was conducted to clarify the actual states of pesticide poisoning (disorders) and its related factors amang 178 male and 232 female farmers.
The results were as follows.
1) Of the subjects 30.7% complained of some health disturbance from pesticides, without significant difference in prevalence between males and females.
2) The most comman symptom was skin hazard (39.7%), followed by nausea (34.9%) and headache (32.5%).
3) Captafol (21.4%) and methomyl (12.7%), which are contained in common pesticides, were recognized as the cause of poisoning.
4) The highest prevalence of health disturbance from pesticides were seen in [orange only] growers (72.7%) among mixed crop groups, followed by [rice+ orange] producers (71.4%), and [rice+ sugar cane +sweet potato] cultivators (55.9%). These higher prevalence groups were also estimated to be the users of the higher amount of pesticide.
5) Significant coefficient correlations were seen between the prevalence of health disturbance from pesticides and the duration and yearly times of pesticide spraying and daily spraying hours.
6) A relatively larger proportion of farmers has a knowledge about pesticide poisonig. However a few of them take effective protective measures at pesticide handling.
The reverse correlation was seen between the prevalence of health disturbance from pesticides and the degree of reliance on effectiveness of pesticides.
The present study indicates that more effective education for safty use of pesticides to both producers and users, and development of non-pesticide use agriculture are essential to protect agricultural workers from health distubance from pesticides.

The outbreak of skin hazards from okra cultivation was studied by a field survey. About a half (46.2 %) of 186 workers investigated (male, 76 ; female, 110) reported to have experienced pastly or annually skin hazards from okra cultivation.The sites of lesion complained were mostly the arms (47.5%), neck (41.3%), fingers (32.5%) and so forth in total number. The degree of lesions were generally itching (85.0%) and flare (61.3%), but severe cases like vanishing fingerprints (16.3%) and fissures on the fingers (11.3 %) were also revealed. Portion of okra responsible for skin hazards seemed to be mainly leaves, trichomes and pods.
Positive patch test reactions with preparations of immature okra pods in 46 workers (male, 12 ; female, 34), compared to 112 control subjects (male, 84 ; female, 28), were significantly higher in okra workers than in controls, which were 25% in males and 20% in total group.
These results show that okra components cause irritant contact dermatitis and allergic contact dermatitis as well.

In order to clarify the actual conditions and mechanism of contact dermatitis from pesticides, a field survey including patch testing with 11 pesticides and formaldehyde was carried out on 111 subjects who engaged in farming in the plastic greenhouse mainly. The relationship between skin sensitization due to pesticides and conditions of pesticide exposure was also studied. The results were as follows;
1) About half of subjects (52%) have been suffered from health hazards due to pesticides. Among the symptoms complained, dermatitis was most frequent (32%) as compared with acute poisoning such as headache (12%), general fatigue (9 %) and so on. The causative pesticides of those dermatitis were mainly Difolatan (72%), Daconil (8 %), and Gramoxone (8%).
2) Relatively higher rates of positive reaction of patch testing were observed in Difolatan (43%) in male, and Difolatan (25%), Topsin M (24%), Rovral (24%) in female. A positive reaction with more than one substance tested were seen in 31 males (55%) and 31 females (55%).
3) Among 11 kinds of pesticides tested, positive correlations between the skin reaction of patch testing and the use of corresponding pesticides were found only in Orthocide (r = 0.233) and Daconil (0.203).
4) From the Cluster Analysis, 16 kinds of using pesticides were classified into 3 clusters with similarity to patch test reactions. It was also suggested from the Dendrogram that there might be cross reactions among patch testing pesticides.
5) By Multiple Regression Analysis, no factor which contributed remarkably to skin sensitization was clarified. And, it was suggested that many kinds of factor were complicatedly related to skin sensitization.

An epidemiological survey on allergic conditions of 58 male (aged 38.6±11.8yrs) and 47 female (aged 42.2 ± 9.6yrs) farmers engaged in growing chrysanthemums.
Among the subjects, 47% of males and 62% of females had complaints of at least one of inquired allergic symptoms associated with the work. The immediate type of allergic reaction on nasal and/ or upper respiratory system raised in the process of sorting flowers in the working room and delayed ype of contact dermatitis in pinching the lateral bud of chrysanthemums or spraying agricultural chemicals in the field were the main complaints of those farmers.
Incidence of positive reaction for patch testing to six kinds of chrysanthemums was 5% for males and 17% for females and that of eight kinds at agricultural chemicals was 34% and 45% for each sex. And relatively high incidence was also observed in those subjects from other immunological tests such as determination of serum immunoglobulin, prick testing and calculation of eosinophil in blood and rhinorrhoea.
There were 39 (67%) males and 36 (77%) females who had at least one of the abnormal finding of those test items above mentioned. However, not the particular kind of Chrysanthemum cultivated in that area was found to be strong causative allergen for the observed allergic conditions.
From these results, it is indicated that relatively high incidence of allergic disorders may be found in the chrysanthemum growers due to inhalation and/or contact of some kinds of allergens introduced from the process of growing to forwarding, such allergen as Chrysanthemum itself, agricultural chemicals and other elements.

The physical work load of farmers and dust conditions in the harvesting process of chrysanthemums were investigated for two households (seven workers for A and three for B household, respectively) at mid March in 1984.
The total working hours a day of each household were 3225 min (460 min as average per a worker) for A and 1954 min (652 min) for B, being allotted more times to males than females. However, all of the house keeping hours were carried out only by females.
The intensity of each working load of farmers was not so heavy, as “light” and “moderately heavy” by Christesen's criteria and energy expenditure a day was calculated 2895-3604 kcal for males and 2155-2295 kcal for females.
In this working process farmers were enforced to keep careful handling with chrysanthemums and fixed body posture for 4 to 6 hours a day. Particulary, the working posture of sorting and bundling chrysanthemums may cause over strained load to the muscle and joint of the lower back and legs.
The dust concentrations of the sorting room were not so high. However, the farmers were exposed a large quantity of cilia of chrysanthemum leaves, calculated 37-259 pieces/cm² on the slide grass a day. The numbers of cilia varied with amounts of handling chrysathemums.
It was suggested that the cilia of chrysanthemums may be the most important allergen to the immediate type of allergy of the chrysanthemum growers.

In order to observe the actual work types and the work load of female farmers in various kinds of agriculture that have gradually become a large part of agriculture in Japan as a result of changes in the structure of agricultural production, the authors performed a questionnaire and interview with a large number of female farmers (2, 113) and women in non-agricultural households.
Greenhouse farming was the most common work type for female farmers, followed by tobacco cultivation. The jobs conventionally undertaken by men, that have required a large dynamic work load, have been progressively mechanized, while auxiliary work before and after machinary work remain as manual labor by women. Some clear differences in the work type between full-time farming and part-time farming of female farmers were observed. On the other hand, it does not seem to be easy to estimate the work strain due to the work loads as mentioned above.

In the farm household of “vegetable planting by plastic green house”, “mandarin orange”, “rush (igusa)”, “dairy-farming” and “silk worm”, measurement of energy expenditure in agricultural work and analysis of daily working hours were conducted at the busy season of each crops. The results are as follows:
The energy consumption per day are estimated respectively to be 3300 Cal for male and 2900 Cal for female in the household of the “plastic green house”; 3500 and 2500 Cal in the “mandarin orange”, 3800 and 3600 Cal in the “rush”, 3300 and 2700 Cal in the “dairy-farming”, 3400 and 2700 Cal in the “silk worm”.
The agricultural machines and tools are mainly operated by male and so attached muscular works not yet machanized are carried by female. And management of animals or crops specially required prudent care have a tendency to be also carried by female. Then the average relative metabolic rate (RMR) a working day is relatively higher in female than male, except “mandarin orange”.
In the “rush harvesting” and “dairy-farming”, having the most development of the agricultural mechanization it seems obviousely to be rising the labor efficiency, but because of expansion of management scale and decreasing number of employee the reduction of work load for a worker is not necessory obvious.

A field study on the hourly working pattern, energy expenditure, air conditions and the testing for fatigue of the process of the raising silk worms for 4 days in August, the period of early autumn raising. Three couples of the typical raising silk worms households in Kikuchi district in Kumamoto Pref. were selected for the study. The results were as follows.
1. In Kikuchi district, there were 5-10 raising periods in a year from april to november for each households and the yearly working days for the process were to be 160-260 days.
In the period of early autumn raising, the average working times and energy expenditure a day were calculated to be 374±136 min. and 2891±277 kcal for males and 383±119 min. and 2494±91 kcal for females in the third state of period (after 5 days of accepting the larva); and in the fifth state of the process (after 10 days of the former state) 644±112 min. and 3087±136 kcal for males, 618±65 min. 2851±48 kcal for females; in the state of the spinning cocoons, 944±238 min. and 3735±459 kcal for males, 846±271 min. and 3209±754 kcal for females; in the state of the controlling cocoons, 560±115 min. and 2944±233 kcal for males, 478±68 min. and 2537±101 kcal for females. In the spinning state it was the highest work loads to be taken for both males and females through one raising period.
2. The RMR (relative metabolic rates) values of each elemental work were measured to be 0.6-3.7 (0.0322-0.0995 kcal/kg/min). The average RMR in a working time was 2.2±0.3 (1.7-2.9) for males and 2.1±0.3 (1.4-2.5) for females, and in the spinning state higher average value was observed for females than for males.
3. There was some difference between males and females in the distribution of working posture. For males 30.8%(9.5-46.2%) of all working times was the standing position, but in the contrally, for females, 66.0%(64.0-69.8%) was the half sitting position and only 11.0% was the standing position.
4. The considerable reduction of flicker frequency was observed at the end of working a day in each state of the period especially in the spinning state, and the increasing of complaints of fatigue symptoms was also observed.

Mass health survey was conducted to discuss the relationship between obesity and some factors of agricultural managements and living and working conditions among 349 pairs of housewives and their husbands selected from the rural communities in Kumamoto prefecture.
The prevalence rates of obesity (20% over of the Minowa's standard weights) were 13.1% for housewives of farmers, 13.7% for their husbands and 15.6 % for housewives of non-farmers, 9.2% for their husbands. The significant correlationship was found in the degree of obesity by Minowa's standard between housewives and their husbands (r= 0.1566, P<0.05).
Being compared the prevalence of obesity according with the kinds of each household's producting crops, peoples of the household of “shiitake: cortinellus shiitake”, “vegetables by plastic greenhouse”, “tabacco” and “animal husbandary ” were more likely to be obese and, inversely, those of “barley”, “vegetables in upland” and “sericulture” were less, than those of “rice”, “mandarin orange” and “forestry”. In the household of “vegetables by plastic greenhouse”, “rice”, “barley”, “mandarin orange” and “vegetables in upland” husbands were more obese than housewives and, inversely, in the household of “shiitake” and “forestry” housewives were more obese than husbands.
Peoples of fishing households were less likely to be obese than those of the farm households and/or other profession. Among the households of non-farmers it was noteworthy that higher prevalence rates of obesity were found in housewives than husbands, being inverse finding in the farmers case.
The results suggested that the agricultural management and it's labor conditions, as well as the level of agricultural economics, might strongly affect the occurrence of obesity in the Japanese rural community.