In order to study the alkaloids from branches and leaves of Ervatamia hainanensis, silica gel, ODS, Sephadex LH-20 and HPLC chromatography were used to obtain six alkaloids from the branches and leaves of E. hainanensis with use of. Based on the physicochemical properties and spectral data, their structures were identified as 10-hydroxydemethylhirsuteine(1), 3R-hydroxycoronaridine(2), 3-(2-oxopropyl)coronaridine(3), pandine(4), 16-epi-vobasine(5), and 16-epi-vobasinic acid(6). Among them, compound 1 was a new monoterpenoid indole alkaloid, and compounds 5 and 6 were obtained from this plant for the first time.
Alkaloids ; Chromatography, High Pressure Liquid ; Molecular Structure ; Plant Leaves ; Tabernaemontana

Four unreported monoterpene indole alkaloids, tabernaecorymines B-E (1-4), together with twenty-one known indole alkaloids (5-25) were obtained from the stem bark of Tabernaemontana corymbosa. Their structures and absolute configurations were elucidated by extensive spectroscopy, quantum chemical calculations, DP4+ probability analyses and Mo₂(OAc)₄-induced electronic circular dichroism experiment. The antibacterial and antifungal activities of these compounds were evaluated and some of them showed significant activity against Staphylococcus aureus,Bacillus subtilis, Streptococcus dysgalactiae and Candida albicans.
Tabernaemontana ; Anti-Infective Agents ; Antifungal Agents ; Anti-Bacterial Agents ; Indole Alkaloids

AIM: To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice. METHODS: Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4). RESULTS: The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1: 56.1% at 506.2 μg·cm(-2); F3: 57.3% at 289.3 μg·cm(-2); and F4: 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction. CONCLUSIONS The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
Animals ; Anti-Inflammatory Agents ; administration & dosage ; Edema ; drug therapy ; Humans ; Male ; Mice ; Plant Extracts ; administration & dosage ; Plant Leaves ; chemistry ; Tabernaemontana ; chemistry

AIM: To evaluate the anti-fertility effect of methanolic (MeTD) and aqueous (AqTD) flower extracts of Tabernaemontana divaricata in rats. METHODS: The anti-fertility activity of the extracts was evaluated using two experimental animal models: 1) Estrogenic activity was carried out in immature female rats using ethinyl estradiol as standard. The evaluation parameters includes changes in uterine weight and histopathology of uterus. 2) Anti-implantation and early abortifacient activity was performed in female Wistar rats. The number of implants and resorbtions were compared to vehicle control. RESULTS: Phytochemical analysis of MeTD and AqTD revealed the presence of carbohydrates, amino acids, steroids, glycosides, flavonoids, alkaloids and tannins. In estrogenic activity, the MeTD and AqTD were offered significant estrogen-like activity at 500 mg·kg(-1), p.o. by increasing the uterine weight compared to vehicle control group. In Anti-implantation and early abortifacient activity study, MeTD (500 mg·kg(-1), p.o.) showed significant effect and it was evident by decrease in the number of implants and increase in the number of resorbtions compared to vehicle control group. CONCLUSION The MeTD at 500 mg·kg(-1), p.o. possess significant estrogenic, anti-implantation and early abortifacient activity, while the AqTD at 500 mg·kg(-1), p.o. was found to possess significant estrogenic activity and the results are in consistent with the literature reports related to anti-fertility effect of flower extracts of Tabernaemontana divaricata.
Abortifacient Agents ; pharmacology ; Animals ; Embryo Implantation ; drug effects ; Female ; Fertility ; Flowers ; chemistry ; Humans ; Models, Animal ; Organ Size ; drug effects ; Phytoestrogens ; pharmacology ; Plant Extracts ; chemistry ; pharmacology ; Rats ; Rats, Wistar ; Tabernaemontana ; chemistry ; Uterus ; drug effects