1.The study of toxicity (LD50) and immunomodulatory effect of antidiabet-3 preparation
Batgerel L ; Ambaga M ; TSend-ayush D
Mongolian Medical Sciences 2011;172(2):125-128
Introduction: There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals.Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset diabetes. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studies, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effect at relatively low cost. Materials and Methods: The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. The Prozorovski3 quick method for the determination of LD50 in the water (20%) and ethanol extractions (30%) of Antidiabetes-3 preparation (AD3). The tested animals were the white mice. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) 4 methodology of studying effects on immune system, we have Antidiabetes-3 preparation (AD3) were given to the 33 mice 2 times a day in 3ml/200gr dose, during 7 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre to screen RBC antibodies.Results: The method developed by V.B. Prozorovski for the calculation of average lethal number was used on 40 white mice (18-22g). Water extraction (10%) was per fused in the tail vein of the experience mouse and the lethal dose (LD50) was 88.9g/kg. These facts prove that the toxic effect of the AD is low. The water (10%) extractions of “Antidiabetes-3” (AD3) preparation were given to the mice 2 times a day in 3ml/200g dose, during 8 days. We have studying compared group “Salimon and Immunal mixture” (S&I) to the mice 2ml/200g dose, during 8 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and hemagglutinin to screen RBC antibodies (Table 1). Figure 1 demonstrates increase in mice’s spleen weight on the 5th day after stimulation of immunity with sheep’s RBC antigen. Spleen weight increase in AD3 group was 1.6 times higher compare to control group (AD3 group 0.16±0.08; control group 0.10±0.02; p<0, 05), and AD3 group was 1.0 times level compare to control group (AD3 group 0.16±0.08; S&I group 0.17±0.09; p<0, 05). In figure 2, the spleen index in control group was 1.24 times higher than in normal group (control group 0, 0047±0.001; normal group 0.0038±0.0004; p<0, 3), AD3 group’s index was 1.3 times higher compare to control group (AD3 group 0.0061±0.002, control group 0, 0047±0.001; p< 0.05), and 1.0 times lower compare to S&I group (AD3 group 0.0061±0.002; S&I group 0.0062±0.003; p< 0.05). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5 *106/ml. AD3 group’s spleen cell number was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutination titre of AD3 group was 1.13 times higher (AD3 group 54.86±19.95%; control group 50±8.83%, p<0,05) and this indicates that BV has immunity stimulating effect.Conclusions:1. Was defined the Antidiabet-3 preparation LD50, 88,9g/kg, its toxicity of classification (Sydorov K.K 1973) was little toxicity.2. Was defined to immunity stimulating effect the Antidiabet-3 preparation
2.Some aspects of traditional Mongolian medicine research
Purevjav M ; Ariunaa Z ; Chimedsuren O ; Tsend-Ayush D ; Burmaajav B
Mongolian Medical Sciences 2014;168(2):61-66
BackgroundTraditional Mongolian Medicine has a history of over 5000 years. Scientific development of TM hasstarted in 1959. Since 1999 Mongolia was categorized by WHO as a country having an Integrativesystem of TM- officially recognized and incorporated into all areas of health care provision, TMMresearch has been following key objectives of National R&D programs.AimIn order to assess the situation of TMM development we have conducted this study based on last10 years’ research done.Ìaterial and MethodsDocument study- we have selected key TMM’s R&D project implementers’ archive and humanresources documents.Descriptive and Analytic methods- a survey of 32 questions evaluating participation of TMMprofessionals in R&D work were conducted. Also, to clarify the point of view about TMM’s R&D6 focus group meetings with different level participants, such as professional committee, policymakers and research workers as well as health care providers, were organized.ResultsFrom 2004-2013, there are 28 projects implemented on TMM, 43% accomplished by TMMRTC,32.8% of which is resulting in raw materials standardization and technology study, related clinicalstudies standing 20% out of all studies done on TMM matter. These numbers are confirmed bysurvey and focus group interviews, more than 50% of participants willing to conduct a clinical studyand expressing difficulties such as lack of knowledge of methodology, policy support and revenue.Conclusions:1. TMM R&D has a potential growth due to human resources capacity. Practitioners are leastinvolved in R&D, due to lack of knowledge of methodology and revenue.2. There were 28 projects implemented on TMM matter, most of these are basic studies, fewerclinical studies done, resulting in pharmacopeia monographs and technological guidelines.
3.Overview of study traditional Mongolian prescription Sugmel-7
Tongshan ; Mandakh ; Tsend-Ayush D ; Enkhtsetseg J ; Wanghuan
Mongolian Pharmacy and Pharmacology 2019;14(1):19-23
Abstract
This article included overview of study of Traditional Mongolian prescription Sugmel-7.
The uses of traditional medicine Sugmel-7 collected by Classic books of Mongolian traditional
medicine, Prescription of Mongolian traditional medicine textbooks. Overview modern medicine
study of Sugmel-7 searched by online Chinese fund of knowledge, research materials of Inner
Mongolian University of nationalities. It would be provided traditional prescription Sugmel-7 future
study clarification.
4.Utilizing lactic acid bacteria for the cultivation of health-safe vegetables
Anuujin G ; Munkhjargal B ; Tsend-Ayush D ; Gerelmaa E ; Baigalmaa J
Mongolian Journal of Health Sciences 2025;88(4):67-70
Background:
Research on lactic acid bacteria has confirmed how specific strains possess probiotic properties and impart
unique sensory characteristics to food products. The use of probiotic lactic acid bacteria in many food products, thus con
fers various health benefits to humans when they are frequently consumed in adequate amounts.
Aim:
To determine the effect of lactic acid bacteria on the growth of tomatoes.
Materials and Methods:
The lactic acid bacteria were cultured using the Lactobacillus medium from whipping cream
and Dandelion (Taraxacum mongolicum) and identified using the MALDI-TOF MS automated microbial identification
analyzer. A solution was prepared using Lactobacillus delbrueckii isolated from whipping cream and Lactobacillus gas
seri isolated from Dandelion (10^7CFU/ml), and sterilized tomato seeds were watered for 10 days with the solution, while
sterilized distilled water was used as a control. The germination rate of the seeds and the root length were measured and
recorded every day.
Results:
The solution of L.delbrueckii bacteria isolated from cream germinated 100% of the seeds, which is 4% higher
than the control seeds, while the solution of L.gasseri bacteria isolated from Dandelion germinated 100%, supporting 4%
higher than the control seeds. Seedlings irrigated with the L.delbrueckii bacterial solution exhibited an average length of
10.3cm, which was 1.3cm longer than the control (P=0.003), indicating a statistically significant difference. Similarly,
those treated with the L.gasseri solution had an average length of 11.5cm, 2.5cm longer than the control (P=0.005), also
demonstrating statistical significance.
Conclusion
The application of the lactic acid bacterial solution significantly enhanced both the germination of tomato
seeds and the growth of the plants compared to the control solution.