Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

Objective:To introduce the use of fluoxetine in neurologic disease,especially in epilepsy.Method:We used fluoxetine as a supplement antiepileptic drug in 25 patients who couldnt be controlled yet by routine antiepileptics. A long term follow-up with these cases was carried out.Results:Most patients got better outcome.Conclusion:Fluoxetine would be an important supplement antiepileptic drug and have value for further clinical and experimental research.

Humans ; Lung Neoplasms ; chemically induced ; Proportional Hazards Models ; Radon ; adverse effects

Retinal detachment (RD) is one of the most common sight-threatening retinal diseases.Visual acuity is not always restored after successful reattachment of the retina.Photoreceptor cell death has been thought to be the major cause of vision loss after RD through various pathways and their interactions,including caspase-dependent apoptosis,endoplasmic reticulum stress-induced apoptosis and non-apoptotic death forms,such as necroptosis,autophagy,and so on.Based on the understanding of the mechanism of photoreceptor cell death after RD,current efforts on combinatorial inhibition of multiple death pathways,targeting upstream molecules and enhancement of intrinsic neuroprotection would help us to protect photoreceptors effectively and improve visual acuity of RD patients.This review summarized current opinions on mechanisms of photoreceptor cell death and the potential treatment strategies.

To promote the normalization,standardization and systematization of hospital infection control,the Coordination and Advisory Group for Hospital Infection Control of the province carried out an investigation in thirty-five 2nd grade hospitals of fourteen prefectures and cities in regard to the orga- nization and management of hospital infection control,necessary infection monitoring and sterilization devices against highly infectious areas through questionaires,medical theory tests and on-the-spot inves- tigations.Analysis is also made of the present conditions and pending problems in the field of infection control of the 2nd grade hospitals in the province.Relevant solutions are suggested.

Breast feeding is the optimal food for all babies. Human milk can promote the immune system and other immature organs of premature infants, and improve the development and long term outcomes. However, breast milk may carry pathogenic microorganisms especially cytomegalovirus, which can be infected through breast feeding. Infected preterm infants could appear a series of clinical symptoms and may developed a series of adversely long-term prognosis. This paper discussed the research progress about preterm infants acquired the cytomegalovirus by breast feeding after birth and focused on the existing clinical prevention treatment strategies.

Objective: To investigate effects of glucose and free fatty acid at different concentrations on phosphorylation of adenosine-5′-monophosphate activated protein kinase(AMPK) and acetyl CoA carboxylase(ACC) in INS-1 cells,and effects of globular adiponectin on phosphorylation of AMPK and ACC.Methods: INS-1 cells were cultured and treated with 5 mmol/L glucose or 0.25 mmol/L free fatty acids,and time courses and dose responses of different dosages of glucose and fatty acid on phosphorylation of AMPK and ACC were measured.We measured the effects of the pharmacological AMPK activator AICAR(5-aminoimidazole-4-carboxamide-riboside) and globular adiponectin on phosphorylation of AMPK and ACC.Results: Glucose and fatty acid at different concentrations inhibited the phosphorylation of AMPK and ACC at the end of 60 min,but AICAR increased the phosphorylation of AMPK and ACC significantly,while 2.5 mg/L globular adiponectin increased the phosphorylation of AMPK and ACC by 23%(P

For HER-2 positive breast cancer, HER-2-targeted agents combined with agents that target downstream signaling or alternative pathways, and endocrine therapy has been investigated in clinical settings. This paper focuses on studies involving phaseⅡand phaseⅢclinical trials for new targets with enhanced clinical applications, including novel small molecular targeting agents, monoclonal antibody-targeted drugs, monoclonal antibody conjugates, dual target drug combination therapy, drug targeting combined with endocrine and targeted chemotherapies, trastuzumab subcutaneous injection, and HER-2 tumor vaccine.

Taking the Fourth Affiliated Hospital of China Medical University as an example,the information integration platform of the hospital is constructed in accordance with the national data and information interaction standard and HL7 standard.The paper introduces the idea for selecting platform products,and technical route and effect of platform construction,and provides guarantee for sustainable development of the hospital,in order to greatly improve core competitiveness of the hospital.

Objective: This paper intends to explore the pattern of training rural general practitioners.Methods: According to the situation of the rural practitioners,this paper designs a questionnaire to collect relative data,sorts and analyzes the data with EXCEL.Results: At present,rural practitioners urgently require the general medicine education,and there exists great room for the training of rural general practitioners.Conclusions:this paper suggests the orientation and a proper course setting of the rural general practitioners education.