Objective:To study the effect of therapeutic alliance of radiofrequency ablation(RFA) and high-frequency thermotherapy(HFH) on the invasion of cony liver cancer cell line VX2.Methods:The cony liver cancer VX2 model was prepared by transplantion,according to the different treatment methods,the models were divided into four groups:Control group (group A 8);RAF group (group B 8),HFH group (group C 8);The therapeutic alliance of RFA+HFH group (group D 8). Results:(1)Gross tumor volume:the tumor volume of group B and D deflated significantly post-treatment 14 days,the group B and D compared within groups(P

Objective To analyze the epidemiological characteristics of public health emergencies and the situation of report and control of public health emergencies in Qijiang District of Chongqing M unicipality during 2009-2013 to provide a scientific ba‐sis for the prevention and control of public health emergencies .Methods The descriptive epidemiology method was used to analyze the directly network reported data of the public health emergencies in Qijiang District during 2009-2013 from the National Emer‐gency Public Health Emergencies Reporting Management System .Results Total 47 public health emergencies were reported in Qi‐jiang District during 2009-2013 ,including 994 cases of caused disease and 1 case of death (food poisoning) .Most of the emergen‐cies were caused by infectious diseases events ,accounting for 82 .78% (39/47) ,food poisoning and other public health emergencies (dog bites) accounted for 2 .13% (1/47) and 14 .89% (7/47)respectively .Respiratory disease events accounted for 78 .72% (37/47) of total infectious diseases events ,the majority were influenza A (H1N1) flu and epidemic parotiditis .The high‐incidence seasons were from April to May(accounting for 34 .04% )and October(40 .42% ) .The most cases happened to the school (especially infec‐tious diseases) ,accounting for 80 .85% of the total events .Conclusion The respiratory infectious disease in the prevailing season is the priority of prevention and control on public health emergencies in Qijiang District .Attentions should be highly paid to the pre‐vention and control of public health emergencies (especially in rural schools);meanwhile ,the dog management and propaganda of prevention and control knowledge should be strengthened to effectively eliminate the potential of hydrophobia .

Objective:To study the inflammation stress reaction between laparoscopic operation and open operation in treating hysteromyoma.Methods: hysteromyoma patients in our hospital from January 2011 to December 2012 were enrolled and randomly divided into observation group given laparoscopic operation and control group given open operation. Then inflammatory factors levels were detected, operation trauma index were observed and the correlation were analyzed.Results: (1)After treatment, inflammatory factor levels of two groups were higher than those before treatment and IL-1(t=5.892,P<0.05), TNF-α(t=11.842,P<0.05)levels of observation group were lower than control group, the difference was statistical significance(P<0.05); (2) There was no significant difference between two groups of operation time(t=0.932,P>0.05), intraoperative bleeding volume(t=7.842,P<0.05), postoperative anal exhaust time(t=7.283,P<0.05), bed time(t=8.213,P<0.05) and NRS pain scores(t=7.883,P<0.05) of observation group were lower than control group(P<0.05); (3) Intraoperative bleeding volume, postoperative anal exhaust time, bed time and NRS pain scores were positively correlated with IL-1,r2 were separately 0.485, 0.521, 0.442, 0.451; and positively correlated with TNF-α levels,r2 were separately 0.556, 0.513, 0.423, 0.458, the difference was statistical significance(P<0.05).Conclusion: Laparoscopic operation is helpful to reduce the operation wound and relieve postoperative inflammatory reaction; and there was close correlation between inflammatory stress and operation trauma.

OBJECTIVE:To facilitate healthy development of Chinese medical industry’s original equipment manufacture(OEM).METHODS:In view of the macro-and micro-environments in China,the new tendency of Chinese medical in?dustry’s OEM and its practical importance were discussed.RESULTS&CONCLUSION:The government should evolve laws and regulations to create satisfactory macro-environment;meanwhile,enterprises should strengthen inner management to find a practical way tailored to their own development.

OBJECTIVE: To lower the price of patent medicine effectively and to increase the access to medicine when public health crises occur. METHODS: Based on the real situation in China, reasons for the inapplicability of compulsory license system for medicine patents were analyzed. RESULTS & CONCLUSION: Chinese government should avoid bulwark of patent and try to explore effective ways to improve the macroscopic policy environment and encourage enterprises to boost up their own strength.

Objective To investigate clinical effect of sparfloxacin on the treatment of ureaplasma urealyticum prostatitis.Methods 96 cases with ureaplasma urealyticum prostatitis, which admitted in our hospital, were selected as the research object and divided into observation group ( n =48 cases) and control group ( n=48 cases) randomly.The control group was given the ofloxacin for treatment and the observation group was given the sparfloxacin for treatment.The clinical effect of the two groups after treatment was analyzed.Results The total effective rate of the observation group (97.92%) was significantly higher than that of the control group (72.92%) (P<0.05).After treatment, the total incidence of adverse reactions of the observation group(8.33%) compared with that of the control group (10.42%), had no significant difference(P>0.05).Conclusion Patients with ureaplasma urealyticum prostatitis, the treatment effect of sparfloxacin is significant, with lower adverse reactions rate and the security is higher, which is recognized in the medical community, can be worthy of popularization and application in clinic.

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Objective To investigate the value of magnetic resonance imaging of heart in diagnosis of unstable angina pectoris.Methods A total of 24 patients with unstable angina pectoris including 15 male and 9 female confirmed by coronary angiography (CAG) were enrolled.All 24 patients were scanned by cardiac magnetic resonance imaging ( CMR),and then the analyses of the first pass images and the delayed contrast enhancement images were carried out for comparison.The findings of cardiac structure,cardiac function,myocardial ischemia,myocardial necrosis,myocardial edema were taken for answering to the images made by echocardiography (UCG) and coronary angiography (CAG) linked with conventional examinations for the diagnosis of coronary disease.Results Compared with the UCG,CMR provided more detailed information about the right ventricle,and the information about left ventricular structure and function given by UCG was very good consistent with that offered by CMR,and CMR could detect myocardial ischemia,myocardial edema and the myocardial necrosis,which were of good consistency with findings often observed by conventional inspection methods.Conclusions Cardiac magnetic resonance imaging (CMR) is of high value in diagnosis and evaluation of unstable angina pectoris and is far superior over other conventional methods for examinations of unstable angina pectoris.

Objective To investigate the psychological state of patients with aplastic anemia. Methods 54 patients with aplastic anemia and 50 health persons were tested with the questionnaires of SAS and EPQ. Results The score of anxiety and the incidence of anxiety in aplasitc anemia patients were much higher than that in health persons. The score of N in the EPQ was much higher in aplasitc anemia patients than that in health persons. Conclusion Patients with aplastic anemia obviously have the incline of nervosity. It is the nurse' duty to adjust patients' negative emotion to help them recovering from the disease.

AIM:To research the effect of safflower in renal intertistial fibrosis by GFR and renal biopsypathology with Sprague-Dnwley(SD) rat model.METHODS:All rats were distributed into 6 groups.Group A was normal control,group B received sham operation,group C was vehicle control,group D received vehicle,group E received safflower(10(g?kg~(-1)) body(wt?d~(-1))),and group F received enalapril((12.5)(mg?kg~(-1)) body(wt?d~(-1))).Rats underwent reversible left renal vein obstruction for 6 d,after which the obstruction was relieved.Renal function was determined by measuring GFR with inulin clearances.Both renal tubular epithelial cells(RTECs) and fibronection deposition for renal interstitial fibrosis were identified by immunohistochemistry.The positive and negative areas of per slice was determined by a motic medical 6.0 graphical analytical system.Atrophic tubules were determined by measuring the tubule diameter.10 fields per slice were analyzed at ?400 magnification.RESULTS:Return of glomerular filtration rate(GFR) was seen in group E and goup F,which was significantly better than group D(P