OBJECTIVETo optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil.

METHODThe extraction procedure of essential oil from fresh H. cordata was optimized with the orthogonal experiment. Essential oil of fresh H. cordata was analysed by GC-MS.

RESULTThe optimize preparative procedure was as follow: essential oil of H. cordata was extracted at a temperature of 35 degrees C, pressure of 15,000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified.

CONCLUSIONThe optimum preparative procedure is reliable and can guarantee the quality of essential oil.

Aldehydes ; analysis ; chemistry ; Carbon Dioxide ; chemistry ; Chromatography, Supercritical Fluid ; methods ; Freeze Drying ; Gas Chromatography-Mass Spectrometry ; methods ; Houttuynia ; chemistry ; Ketones ; analysis ; chemistry ; Oils, Volatile ; analysis ; chemistry ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry ; Pressure ; Temperature

OBJECTIVETo study the aqueous constituents of Houttuynia cordata.

METHODVarious columns including Diaion HP-20, Sephadex LH-20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis.

RESULTFive compounds were isolated, and their structures were identified as chlorogenic methyl ester (1), (E)-4-Hydroxy-4-[3'-(beta-D-glucopyranosyloxy) butylidene]-3, 5, 5-trimethyl-2-cyclohexen-1-one (2), 2-(3, 4-dihydroxyphenyl) ethyl-beta-D-glucopyranoside (3), p-hydroxyphenethyl-beta-D-glucoside (4), 4-(beta-D-glucopyranosyloxy)-3-hydroxy-Benzoic acid (5).

CONCLUSIONAll compounds were isolated from this plant for the first time.

Chlorogenic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; Chromatography, Gel ; Cyclohexanones ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Houttuynia ; chemistry ; Magnetic Resonance Spectroscopy ; Phenols ; chemistry ; Plants, Medicinal ; chemistry ; Spectrometry, Mass, Electrospray Ionization

OBJECTIVETo study the flavonoid constituents in fresh herb of Houttuynia cordata.

METHODVarious column packing materials including Diaion HP - 20, Sephadex LH - 20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis.

RESULTFive compounds were isolated, and their structures were identified as quercetin-3-O-beta-D-galactoside-7-O-beta-D-glucoside (1), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside (2), quercitrin (3), hyperin (4), quercetin 3-O-alpha-L-rhamnopyranosyl-7-O-beta-D-glucopyranoside (5).

CONCLUSIONCompounds 1, 2 and 5 were separated from H. cordata for the first time.

Flavonoids ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Houttuynia ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; isolation & purification