OBJECTIVETo investigate the expression of neuron-specific enolase (NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus.

METHODSImmunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age.

RESULTSIn fetuses with 2, 3 and 4 months of gestation, the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38 ± 8.37, 25.00 ± 11.54 and 38.00 ± 15.09, respectively; the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38 ± 14.93, 62.25 ± 18.59 and 56.44 ± 14.70, respectively. NSE-positive cells were detected in the esophageal epithelium only at the third month. In the fetuses at 2, 3 and 4 months of gestation, SYN in all layers of esophageal tissue were positively or strong positively expressed, especially in the myenteric plexus and submucosal plexus. The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69 ± 9.34, 51.84 ± 6.10 and 46.41 ± 6.44, respectively.

CONCLUSIONSYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development.

Esophagus ; embryology ; Female ; Fetus ; Gestational Age ; Humans ; Immunohistochemistry ; Phosphopyruvate Hydratase ; metabolism ; Pregnancy ; Synaptophysin ; metabolism

OBJECTIVE: To observe the GluR2 expression in rat inferior colliculus (IC) in different developmental stages, and to investigate its developmental change and relationship with the synapse development. METHOD: The expression of GluR2 and synaptophysin(SYP) in IC were detected by double immunofluorescence method. RESULT: (1) All sorts of neurons in IC expressed GluR2 in every postnatal groups, and the GluR2 expression in P6w groups was higher than that in other groups. (2) The expression of GluR2 were different in different subnucleus of IC. (3) All sorts of neurons in IC expressed SYP in every postnatal groups, and the SYP expression in P6w groups was higher than others. (4) The expressions of GluR2 consistent with the expression of SYP in IC. CONCLUSION The developmental changes of GluR2 and SYP expression in the rat IC may be involved in the development and plasticity of auditory center.
Animals ; Hypothalamus ; cytology ; metabolism ; Inferior Colliculi ; growth & development ; metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, AMPA ; metabolism ; Synapses ; metabolism ; Synaptophysin ; metabolism

Carcinoid Tumor ; metabolism ; pathology ; surgery ; Female ; Humans ; Middle Aged ; Phosphopyruvate Hydratase ; metabolism ; Synaptophysin ; metabolism ; Thymectomy ; Thymus Neoplasms ; metabolism ; pathology ; surgery

Aged ; Aged, 80 and over ; Alzheimer Disease ; metabolism ; pathology ; Brain ; metabolism ; Dentate Gyrus ; metabolism ; Dynamin I ; metabolism ; Hippocampus ; metabolism ; Humans ; Monomeric Clathrin Assembly Proteins ; metabolism ; Neuropil ; metabolism ; Synaptophysin ; metabolism

OBJECTIVETo investigate the distribution patterns of neuron-specific enolase (NSE) and synaptophysin (SYN) during the development of human fetal stomach.

METHODSSixteen specimens of human fetal (gestational age 2 to 4 months) gastric tissues were examined with immunohistochemistry for detecting the distribution of NSE and SYN expressions in the gastric walls.

RESULTSDuring the second to fourth gestational months, NSE was strongly expressed in the nerve cells and nerve fibers of the myenteric nerve plexus of human fetal stomach. As the gestational age increased, the numbers of NSE positive cells and fibers increased gradually in the gastric submucosa, but NSE was negative in the gastric mucosa. At the second gestational month, SYN expression was negative in the mucosa but positive in the myenteric nerve plexus; during the third to fourth months, positive SYN expression was found in the mucosa, submucosa and myenteric nerve plexus of the embryonic gastric walls and its expression intensity increased with the gestational age.

CONCLUSIONSYN and NSE are both involved in the regulation of the nervous system in the gastric wall but their expressions and distributions follow different patterns during the development of human fetal stomach.

Fetus ; metabolism ; Gastric Mucosa ; metabolism ; Gestational Age ; Humans ; Immunohistochemistry ; Myenteric Plexus ; Nerve Fibers ; metabolism ; Neurons ; metabolism ; Phosphopyruvate Hydratase ; metabolism ; Stomach ; metabolism ; Synaptophysin ; metabolism

Adult ; Chromogranin A ; metabolism ; Female ; Humans ; Immunohistochemistry ; Pancreatic Neoplasms ; diagnosis ; metabolism ; therapy ; Phosphopyruvate Hydratase ; metabolism ; Somatostatin ; metabolism ; Somatostatinoma ; diagnosis ; metabolism ; therapy ; Synaptophysin ; metabolism ; Tomography, X-Ray Computed

Adult ; CD56 Antigen ; metabolism ; Carcinoma, Neuroendocrine ; metabolism ; pathology ; surgery ; Humans ; Ki-67 Antigen ; metabolism ; Male ; Mediastinal Neoplasms ; metabolism ; pathology ; surgery ; Mediastinum ; pathology ; surgery ; Phosphopyruvate Hydratase ; metabolism ; Synaptophysin ; metabolism

Adolescent ; Cerebellar Cortex ; metabolism ; pathology ; Cerebellar Neoplasms ; metabolism ; pathology ; surgery ; Female ; Follow-Up Studies ; Ganglioneuroma ; metabolism ; pathology ; surgery ; Humans ; Neurofilament Proteins ; metabolism ; Synaptophysin ; metabolism

Adult ; Brain Neoplasms ; metabolism ; pathology ; surgery ; Female ; Ganglioglioma ; metabolism ; pathology ; surgery ; Glial Fibrillary Acidic Protein ; metabolism ; Humans ; Magnetic Resonance Imaging ; Phosphopyruvate Hydratase ; metabolism ; Synaptophysin ; metabolism

OBJECTIVEThe aim of this study was to investigate the clinicopathological features of different histological types of primary gastric neuroendocrine neoplasms (including the esophagogastric junction), and to analyze the characteristics and difficulties in diagnosis of all the subtypes of this disease.

METHODS75 cases of primary gastric neuroendocrine neoplasms (including the esophagogastric junction) were included in this study. The expressions of several markers including somatostatin, synaptophysin, chromogranin A, CD56, S-100, neuron-specific enolase and CD57 were assayed in all the specimens by immunohistochemical staining, and their significance in the diagnosis and prognosis of gastric neuroendocrine neoplasms were assessed. In addition, the relationship between various clinical parameters such as tumor location, histological types, depth of invasion and metastasis was also analyzed.

RESULTSThe incidence of gastric neuroendocrine neoplasms accounted for 1.5% of gastric cancer in the same period, and the proportion of each subtype was 53.3% (40/75) in G3, 29.3% (22/75) in MANEC, 16.0% in G1(12/75), and 1.3% (1/75) in G2, respectively. 41.7% (5/12) of the G1 showed multifocal lesions, accompanyied with neuroendocrine cell hyperplasia in the gastric mucosa. 54.67% (41/75) of the NEN located in the esophagogastric junction. The lymph node metastasis of MANEC is unique. The coincidence rate in diagnosis of preoperative biopsies and postoperative specimen was 75.0% (9/12) in G1, 72.7% (16/22) in MANEC, and 25.0% (10/40) in G3, respectively.

CONCLUSIONSGastric neuroendocrine neoplasms occur mainly in the esophagogastric junction, and most of them were highly malignant. The coincidence rate of preoperative and postoperative pathological diagnosis for primary gastric neuroendocrine neoplasms is low. Therefore, it should be very cautious when diagnosis of this disease is made in a preoperative biopsy.

Chromogranin A ; metabolism ; Esophagogastric Junction ; metabolism ; Gastric Mucosa ; metabolism ; pathology ; Humans ; Lymphatic Metastasis ; pathology ; Neuroendocrine Tumors ; pathology ; Phosphopyruvate Hydratase ; metabolism ; Prognosis ; Stomach Neoplasms ; pathology ; Synaptophysin ; metabolism