Background: Despite the success of the Expanded Program of Immunization, many vaccine-preventable diseases remain prevalent in developing countries. Objective: This study aims to determine the knowledge, attitudes, and practices of parents regarding immunization of their children. Methods: This is a descriptive study using a pre-tested, self-administered questionnaire pertaining to knowledge, attitudes, and practices of parents regarding immunization. The questionnaire was given to parents whose children were being immunized at a barangay health center within a one month period. Results: Twenty-nine (93%) out of 31 mothers claimed they knew what vaccines were to be given, but only 22 (75.86%) were correct. Twenty-one (68.75%) learned of the immunization through barangay health workers. With regard to adverse reactions: giving of appropriate medication was applied by 18 (69.2%) respondents. Six mothers (23%) preferred going to a physician; 29 (93.5%) were aware of the next scheduled dates of visit. Major reasons for missed vaccinations were sickness and long waiting time before vaccine was given: accounting for 9 (29.03%) each. During the times when vaccines were not available at the health center, 19 (61.29%) preferred to wait for it to become available. 20 respondents (64.5%) knew of other recommended vaccines which were not included in the EPI (Non-EPI) vaccines, but only 3 (9.7%) availed of it from private physicians. Of this group, 14 (70%) were willing to avail of the vaccines; 17 (85%) were thought of these vaccines as expensive; 12 (71%) were willing to have their children vaccinated; and 3 (17.64%) opted to save money first prior to vaccination. Conclusion: Parents still lacked knowledge with regards to their children's vaccination. The outcome of the child being fully immunized depends on the availability and affordability of vaccine, as well as, the willingness and effort of their parents.
Human ; Male ; Female ; Infant ; Infant Newborn ; IMMUNIZATION ; VACCINATION ; PARENTS

Objective: To develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings. Methods: In this study, seven different tetracycline treatment methods were conducted to obtain the best removal method. Four methods focused on larvae tetracycline treatment, one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment. Results: All larval tetracycline treatments resulted in either high larvae mortality, sterile F

OBJECTIVETo develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings.

METHODSIn this study, seven different tetracycline treatment methods were conducted to obtain the best removal method. Four methods focused on larvae tetracycline treatment, one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment.

RESULTSAll larval tetracycline treatments resulted in either high larvae mortality, sterile F0 adult mosquitoes or unsuccessful Wolbachia removal. Treatment of both larvae and adults resulted in reduced larvae mortality, successful Wolbachia removal but slow mosquito fecundity. As for the adult treatment, 1.0 mg/mL as previously published was not able to completely remove Wolbachia in F1 generation whereas 1.25 mg/mL successfully removed Wolbachia from F1 and F2 mosquitoes in 2 weeks.

CONCLUSIONSThis method is different from the previously published methods as it provides an improved Wolbachia removal technique from Ae. albopictus with high egg hatchability, low larvae mortality, increased fecundity and better Wolbachia removal rate.