1.Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island.
Song Hak LEE ; Sang Chul KANG ; Dae Yong KIM ; Jong Hee BAE ; Jae Hoon KIM
Journal of Veterinary Science 2007;8(1):51-55
Swine hepatitis E virus (HEV) is an emerging zoonotic pathogen due to its close genomic similarity to human HEV. The prevalence of swine HEV in the hepatic lesion of pigs from the Jeju Island was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). In total, 40 pigs with hepatitis lesions were selected from 19 different farms, based on examination by microscopy. RTPCR findings revealed swine HEV in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. Several histopathological lesions, including multifocal lymphoplasmacytic hepatitis, portal inflammation, and focal hepatocellular necrosis, were observed in liver sections of swine HEV PCR-positive pigs. The present study suggests that the prevalence of swine HEV is very high in the pig population in Jeju Island, and that pigs are infected at early stages of growth (under 2 months of age). The high prevalence of swine HEV in pigs in Jeju Island and the ability of this virus to infect across species puts people with swine-associated occupations at possible risk of zoonotic infection.
Animals
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DNA Primers
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Hepatitis E/epidemiology/pathology/*veterinary
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Hepatitis E virus/*genetics
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Korea/epidemiology
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Liver/pathology
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Prevalence
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Reverse Transcriptase Polymerase Chain Reaction
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Swine
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Swine Diseases/*epidemiology/*virology
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Zoonoses/*epidemiology
2.Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea.
Dong Kun YANG ; Byoung Han KIM ; Chang Hee KWEON ; Jun Hun KWON ; Seong In LIM ; Hong Ryul HAN
Journal of Veterinary Science 2004;5(2):125-130
A virus strain, showing cytopathic effect in Vero cell, was isolated from plasma of a fattening pig in Gyeonggi province, Korea in October 1999. The evaluation of physicochemical/biological properties of the isolate showed that the virus, KV1899, inoculated suckling mouse showed paralysis and died within 7 days post-inoculation, the mouse brain suspension had hemagglutinating activity with goose RBC. Pathogenicity of isolate was carried out by intracranial and intraperitoneal inoculation of 3-4 weeks mice. The mice inoculated with isolate showed 10 4.5 LD50/ 0.03 ml and 10 3.0 LD50/0.5 ml according to the inoculation route. The isolate was identified as RNA and enveloped virus using IUDR and chloroform sensitivity test. The virus particles within the infected Vero cell were measured to be 40-50 nm in size by electron microscopy. The isolate was further characterized by immuno-fluorescence assay using Japanese encephalitis virus (JEV) specific monoclonal antibodies. Reverse transcription polymerase chain reaction (RT-PCR) revealed the presence of JE specific conserved sequences in this isolate. The artificially inoculated pigs had HI titer of 320 to 2,560 against JEV at 14 to 42 days post inoculation. We confirmed this isolate as Japanese encephalitis virus. It was the second isolation of JEV in pigs in Korea.
Animals
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Antibodies, Viral/analysis
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Cercopithecus aethiops
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Cytopathogenic Effect, Viral
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Encephalitis Virus, Japanese/*classification/*isolation & purification/ultrastructure
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Encephalitis, Japanese/pathology/*veterinary/virology
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Fluorescent Antibody Technique, Indirect/veterinary
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Hemagglutination Inhibition Tests/veterinary
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Hemagglutination Tests/veterinary
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Korea
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Mice
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Microscopy, Electron/veterinary
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RNA, Viral/analysis
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Reverse Transcriptase Polymerase Chain Reaction/veterinary
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Swine
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Swine Diseases/pathology/*virology
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Vero Cells/virology
3.Emergence of virulent pseudorabies virus infection in Northern China.
Rui WU ; Chaoyong BAI ; Jinzhong SUN ; Shengke CHANG ; Xuke ZHANG
Journal of Veterinary Science 2013;14(3):363-365
Our investigation was conducted in order to verify a recent severe epidemic at several swine farms in northern China that indicated a newly emerging disease. Evidence confirmed that the epidemic was caused by a virulent Pseudorabies virus infection in swine herds.
Animals
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China/epidemiology
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Enzyme-Linked Immunosorbent Assay/veterinary
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Epidemics/*veterinary
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Herpesvirus 1, Suid/classification/*isolation & purification/*pathogenicity
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Pseudorabies/*epidemiology/mortality/pathology/virology
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Reverse Transcriptase Polymerase Chain Reaction/veterinary
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Sequence Analysis, DNA/veterinary
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Swine
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Swine Diseases/*epidemiology/mortality/pathology/virology
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Vaccination/adverse effects/veterinary
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Virulence