Porcine Torovirus (PToV) is widely distributed in the world with high prevalence rate in swinery. Due to the high detection rate in diarrhea pigs, PToV is thought to be a potential pathogen of swine diarrhea. In recent years, epidemic outbreaks of diarrhea with high morbidity and mortality in China have caused great economic losses. Intertypic recombination events and antigenic cross-reactivity among toroviruses implies potential zoonotic transmission of PToV. The review represented the development history of PToV and made a brief summary of the features in genome and protein epidemiology and laboratory diagnosis of the PToV, and so on.
Animals ; China ; epidemiology ; Swine ; Swine Diseases ; epidemiology ; virology ; Torovirus ; genetics ; physiology ; Torovirus Infections ; epidemiology ; veterinary ; virology

Swine influenza viruses (SIVs) are respiratory pathogens of pigs. They cause both economic bur den in livestock-dependent industries and serious global public health concerns in humans. Because of their dual susceptibility to human and avian influenza viruses, pigs are recognized as intermediate hosts for genetic reassortment and interspecies transmission. Subtypes H1N1, H1N2, and H3N2 circulate in swine populations around the world, with varied origin and genetic characteristics among different continents and regions. In this review, the role of pigs in evolution of influenza A viruses, the genetic evolution of SIVs and interspecies transmission of SIVs are described. Considering the possibility that pigs might produce novel influenza viruses causing more outbreaks and pandemics, routine epidemiological surveillance of influenza viruses in pig populations is highly recommended.
Animals ; Humans ; Influenza A virus ; genetics ; isolation & purification ; physiology ; Influenza, Human ; epidemiology ; transmission ; virology ; Orthomyxoviridae Infections ; epidemiology ; transmission ; veterinary ; virology ; Swine ; Swine Diseases ; epidemiology ; transmission ; virology

Animals ; Global Health ; History, 20th Century ; Humans ; Influenza A virus ; genetics ; isolation & purification ; Influenza, Human ; epidemiology ; history ; mortality ; virology ; Orthomyxoviridae Infections ; epidemiology ; veterinary ; virology ; Swine ; Swine Diseases ; epidemiology ; virology

BACKGROUNDTo investigate the prevalence of anti-HEV among swine, sheep and chickens.

METHODSTotally 498 sera of swine, sheep and chickens collected from Xingjiang, Guangxi, Guangdong, Beijing and Hebei were detected for the anti-HEV by an enzyme linked immunoassay.

RESULTSThe anti-HEV positive rate of swine was 67.53%(104/154), in pigs between 4-5 months of age the rate was 100.00%(9/9) from Xingjiang. The rate in pigs under 3 months of age from Guangxi was 36.00%(9/25) and in pigs older than six months of age was 71.67% (86/120), respectively. The 108 sera of sheep collected from Xingjiang were all negative. The positive rate of chickens was only 1.27% (3/236). The anti-HEV prevalence rates of chickens from Luoding, Shenzhen, Liuzhou, Beijing and Hebei were 4.00%, 1.49%, 1.49%, 0, 0 respectively.

CONCLUSIONHEV infection does exist among swine and chickens. The anti-HEV prevalence of swine was the highest among domestic animals. The role of swine and chickens in transmission of HEV needs to be further studied.

Animals ; Antibodies, Viral ; Chickens ; China ; epidemiology ; Hepatitis Antibodies ; blood ; Hepatitis E ; epidemiology ; veterinary ; Hepatitis E virus ; immunology ; Poultry Diseases ; epidemiology ; virology ; Prevalence ; Sheep ; Sheep Diseases ; epidemiology ; virology ; Swine ; Swine Diseases ; epidemiology ; virology

Most isolates of foot-and-mouth disease virus (FMDV) display a broad host range. Since the late 1990s, the genetic lineage of PanAsia topotype FMDV serotype O has caused epidemics in the Far East, Africa, the United Kingdom, France, the Netherlands, and numerous other countries throughout Europe and Asia. In contrast, there are several FMDV isolates that exhibit a more restricted host range. A Cathay topotype isolate of FMDV serotype O from the 1997 epizootic in Taiwan (O/TAW/97) demonstrated restricted host specificity, only infecting swine. Methods used to evaluate infectivity and pathogenicity of FMDV isolates in cattle are well-documented, but there has been less progress studying transmission and pathogenicity of FMDV isolates in pigs. In previous studies designed to examine pathogenicity, various chimeric viruses derived from O/TAW/97 were intradermally inoculated in the heel bulb of pigs. Subsequent quantitative scoring of disease and evaluation of virus released into nasal secretions and blood was assessed. Here we prove the usefulness of this method in direct and contact inoculated pigs to evaluate infectivity, pathogenicity and transmission of different Asian FMDV isolates. Virus strains within the Cathay topotype were highly virulent in swine producing a synchronous disease in inoculated animals and were efficiently spread to in-contact naive pigs, while virus strains from the PanAsia topotype displayed more heterogeneous properties.
Animals ; Antibodies, Viral/blood ; Disease Outbreaks/*veterinary ; Foot-and-Mouth Disease/epidemiology/transmission/*virology ; Foot-and-Mouth Disease Virus/*pathogenicity ; Host Specificity ; Swine ; Swine Diseases/epidemiology/transmission/*virology ; Taiwan/epidemiology

Animals ; Humans ; Influenza, Human ; virology ; Orthomyxoviridae ; genetics ; isolation & purification ; Orthomyxoviridae Infections ; epidemiology ; veterinary ; virology ; Swine ; Swine Diseases ; virology ; Viral Proteins ; genetics

Since the first report of a swine influenza virus (SIV) infection in humans in 1958, cases have occurred continuously and increased significantly after the 2009 H1N1 pandemic. Although exposure to swine is thought to be a risk factor for human SIVs infections, approximately half of the reported cases had no known exposure to pigs. Besides, epidemiological investigation showed that several cases had limited human-to-human transmission. Based on the analyses of data on swine influenza virus infection in humans in this review, both the improved SIVs surveillance in humans and swine population and wider vaccination coverage among occupational workers are critical strategies in pandemic preparedness and response.
Animals ; Humans ; Influenza A virus ; genetics ; isolation & purification ; physiology ; Influenza, Human ; diagnosis ; epidemiology ; transmission ; virology ; Orthomyxoviridae Infections ; diagnosis ; epidemiology ; veterinary ; virology ; Swine ; Swine Diseases ; diagnosis ; epidemiology ; transmission ; virology ; Zoonoses ; diagnosis ; epidemiology ; transmission ; virology

Porcine epidemic diarrhea virus (PEDV) LJB/03 strain was isolated from the feces of piglets suspected to be suffering from a severe diarrhea in Heilongjiang Province, and was identified by immunofluorescence test, immunelectronmicroscopy, RT-PCR and indirect ELISA assay. Characteristics of the virus culture and the methods of improvement of virus titer were explored. The results showed that the virus had the typical appearance of the coronavirus. Analysis of the nucleotide sequences of RT-PCR products revealed 98% homology with the reference strains. Indirect immunofluorescence assay showed a significant presence of green fluorescence, and an average P/N ratio of 7.6 by indirect ELISA assay. Taken together, these tests showed positive isolation of PEDV. Using the virus plaque purification cloning methods established in the test, the purified PEDV large plaque and small plaque were obtained, and the large plaque and small plaque titers were measured with significant difference. These results provide potential for the application of PEDV on the basis of the biological features of isolated virus.
Animals ; Cell Culture Techniques ; China ; epidemiology ; Coronavirus Infections ; epidemiology ; veterinary ; virology ; Epidemics ; Feces ; virology ; Porcine epidemic diarrhea virus ; genetics ; growth & development ; isolation & purification ; Swine ; Swine Diseases ; epidemiology ; virology ; Virus Cultivation

OBJECTIVETo determine the genotype and phylogenetic characteristics of hepatitis E virus (HEV) strains isolated from the human and swine in Anqing City.

METHODSTwenty seven sera from sporadic hepatitis E patients and 400 commercial swine bile samples were collected in Anqing City. According to the collection time, the bile samples were equally divided into 4 groups which were named group A, B, C and D respectively. Nested reverse transcription polymerase chain reaction (RT-PCR) and DNA sequencing technology were performed to obtain the DNA sequences of HEV RNA Open Reading Frame 2 (ORF2) (150 nt) for all the serum and bile samples. The sample sequences and prototype sequences from the GenBank were aligned and their nucleotide sequence identities were calculated. A phylogenetic tree constructed according to the Bayesian inference method was used to analyze the genotype and phylogenetic relationship between the human and swine HEV strains isolated in Anqing City.

RESULTSThe male-to-female sex ratio of the patients was 2.86:1 and the average age was 56.78 years old. Sixteen out of 27 serum (59.26%) samples were HEV RNA positive. Human HEV strains isolated in Anqing City shared 74.75% - 82.99%, 75.26% - 83.64%, 72.77% - 80.57% and 88.03%-91.63% nucleotide sequence identities with prototype I, II, III and IV HEV strains respectively. HEV RNA was detected in 22 out of 400 bile samples (5.5%). The swine HEV detection rates for group A, B, C and D were 7.00%, 3.00%, 9.00% and 3.00% respectively, showing no significant difference among these groups (χ(2) = 5.20, P = 0.16). Swine HEV strains isolated in Anqing City shared 75.24% - 83.42%, 75.93% - 84.19%, 72.86% - 80.64% and 88.15% - 91.79% nucleotide sequence identities with prototype I, II, III and IV HEV strains respectively. Phylogenetic analysis revealed that all the HEV strains isolated from both the human and swine belonged to genotype IV and scattered in evolutionary branches without significant species aggregation.

CONCLUSIONIt's suggested that genotype IV HEV was the dominant genotype among the human and swine in Anqing City and probably transmitted between them in this area.

Aged ; Animals ; Base Sequence ; China ; epidemiology ; Female ; Genotype ; Hepatitis E ; epidemiology ; veterinary ; virology ; Hepatitis E virus ; classification ; genetics ; isolation & purification ; Humans ; Male ; Middle Aged ; Phylogeny ; Swine ; virology ; Swine Diseases ; epidemiology ; virology

OBJECTIVETo investigate hepatitis E virus (HEV) infection among pigs in Henan province.

METHODSA total of 623 swine sera, collected from 5 districts, were divided into two groups, under 3-month of age and over 3-month of age. They were tested for HEV antigen and antibody by using ELISAs, respectively. The sera positive for HEV antigen were tested for HEV RNA with RT-PCR. The positive products of RT-PCR were cloned and sequenced.

RESULTSThe positive rates of anti-HEV antibody of the groups under 3-month and over 3-month of age were 90.27% and 92.55%, respectively, without statistical difference, while those of HEV antigen were 15.93% and 5.69%, respectively, with significant difference. The positive rates of anti-HEV antibody and HEV antigen were significantly different among different districts. HEV RNA was detectable in 5 of 47 HEV antigen positive samples. The sequence analysis showed that in 4 of 5 specimens the sequence belonged to genotype 4 while in the remaining one the sequence was genotype 1.

CONCLUSIONThe prevalence rate of HEV infection in pigs was high in Henan province and the rate differed in different districts.

Animals ; Antibodies, Viral ; analysis ; immunology ; Antigens, Viral ; analysis ; immunology ; China ; Genotype ; Hepatitis E ; epidemiology ; immunology ; veterinary ; virology ; Hepatitis E virus ; genetics ; immunology ; isolation & purification ; Phylogeny ; RNA, Viral ; analysis ; genetics ; Sequence Analysis, DNA ; Swine ; virology ; Swine Diseases ; epidemiology ; immunology ; virology