Objective To explore the regulatory role of calcium activated chloride channel (CaCC) in vascular structural remodeling in pathogenesis of pulmonary arterial hypertension (PAH) induced by high pulmonary blood flow.Method An abdominal aorta and inferior vena cava shunting operation was used to induce high pulmonary blood flow and establish a PAH rat model.Seventy-five SD rats were randomly divided into normal,sham,shunt,niflumic acid (NFA) 1 (0.2 mg/(kg · d)) and NFA 2 (0.4 mg/(kg · d)) groups.There were 15 rats in each group.Pulmonary artery pressure and vascular structural remodeling were measured,arteriole contraction ratio among these groups were compared using vascular tone analysis system,and the electrophysiology of pulmonary artery smooth muscle cell (PASMC) was recorded using patch clamp technology.Differences between multiple groups were compared through variance analysis and that between groups with q test.Result Compared with normal ((14.4 ± 1.3) mmHg,1 mmHg =0.133 kPa) and sham groups ((13.5 ± 2.3) mmHg),mean pulmonary artery pressure in shunt group ((27.4 ± 2.4) mmHg) increased significantly (P ＜ 0.05).Compared with shunt group,mean pulmonary artery pressure in NFA 1 group ((21.2 ± 2.0) mmHg) and NFA 2 group ((22.3 ± 2.0) mmHg) decreased significantly (P ＜ 0.05).Pulmonary vascular structural remodeling including pulmonary artery stenosis presented in shunt group.Compared with normal ((114.3 ± 1.2) ％) and sham ((115.5 ± 1.1) ％) groups,arteriole contraction ratio to 10-5 mol/L phenylephrine in shunt group ((132.6 ± 1.4) ％) increased significantly (P ＜ 0.05).Compared with shunt group,pulmonary vascular structural remodeling alleviated in NFA 1 and NFA 2 groups.Arteriole contraction ratio in NFA 1 group ((126.4 ± 1.3) ％) and NFA 2 group ((124.6 ± 1.0) ％) decreased significantly compared with shunt group (P ＜ 0.05).Patch clamp technique recorded typical CaCC currents.Compared with normal ((32.3 ±2.3) pA/pF) and sham groups ((35.3 ± 1.2) pA/pF),the CaCC current density of PASMC in shunt group ((51.3 ± 2.7) pA/pF) increased significantly (P ＜ 0.05).Compared with shunt group,the CaCC current density of PASMC in NFA 1 group ((40.2 ± 1.5) pA/pF) and NFA 2 group ((42.7 ± 2.2) pA/pF) decreased significantly (P ＜ 0.05).Conclusion CaCC is involved in pulmonary arterial hypertension induced by high pulmonary blood flow through regulating membrane potential.NFA attenuate pulmonary vascular structural remodeling and pulmonarv pressure through decreasing CaCC current density of PASMC membrane.

Objective To observe the influence of citalopram on anxiety and depression of patient with functional dyspepsia (FD).Methods One hundred and thirty-two FD patients were randomly divided into treatment group (n =68)and control group (n =64).The patients in con-trol group were given omeprazole and mosapride,while the patients in the treatment group were added citalopram based on the treatment in the control group,and meanwhile given corresponding psychology guidance.The anxiety and depression disorders were evaluated respectively by Self-rat-ing Depression Scale (SDS)and Self-rating Anxiety Scale (SAS).The symptomatic scores before treatment and on the second,fourth and eighth week of treatment,clinical efficiency and adverse reactions were observed in the two groups.Results Among 132 patients,there were 60 with de-pression and 53 patients suffering from anxiety.SDS and SAS scores on on the second,fourth and eighth week of treatment and FD symptomatic scores on the fourth and eighth weeks of treatment were obviously lower,while the overall response rate was significantly higher in treatment group than that in the control group.So the difference was statistical significant.Before and after treat-ment,there were no abnormal patients in two groups.Conclusion FD patients are usually accom-panied by anxiety and depression.The application of citalopram on the basis of routine treatment could significantly ameliorate the patients′anxiety and depression,alleviate symptoms,improve the efficiency and reduce adverse reactions.

Objective To observe the influence of citalopram on anxiety and depression of patient with functional dyspepsia (FD).Methods One hundred and thirty-two FD patients were randomly divided into treatment group (n =68)and control group (n =64).The patients in con-trol group were given omeprazole and mosapride,while the patients in the treatment group were added citalopram based on the treatment in the control group,and meanwhile given corresponding psychology guidance.The anxiety and depression disorders were evaluated respectively by Self-rat-ing Depression Scale (SDS)and Self-rating Anxiety Scale (SAS).The symptomatic scores before treatment and on the second,fourth and eighth week of treatment,clinical efficiency and adverse reactions were observed in the two groups.Results Among 132 patients,there were 60 with de-pression and 53 patients suffering from anxiety.SDS and SAS scores on on the second,fourth and eighth week of treatment and FD symptomatic scores on the fourth and eighth weeks of treatment were obviously lower,while the overall response rate was significantly higher in treatment group than that in the control group.So the difference was statistical significant.Before and after treat-ment,there were no abnormal patients in two groups.Conclusion FD patients are usually accom-panied by anxiety and depression.The application of citalopram on the basis of routine treatment could significantly ameliorate the patients′anxiety and depression,alleviate symptoms,improve the efficiency and reduce adverse reactions.