1.A Novel Fluorescence Sensor for Highly Sensitive Detection of Glucose
Aiqin LI ; Chang GUO ; Suying XU
Chinese Journal of Analytical Chemistry 2017;45(6):824-829
A fluorescence nanosensor based on an easily prepared fluorescent molecule, 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD), was developed for highly sensitive detection of glucose.Under the catalysis of horseradish peroxidase (HRP), 3,3′,5,5′-tetramethylbenzidine (TMB) was oxidized into oxidized TMB (oxTMB) by H2O2.And the fluorescence of OPD was quenched by the intense absorption of the formed oxTMB, thus realizing effective quantitative detection of H2O2.The linear range was 0.05-0.8 μmol/L and 1-10 μmol/L respectively, with limit of detection of 0.02 μmol/L.Besides, on the basis of transformation of glucose into H2O2 through the catalysis of glucose oxidase, this nanosensor could be further exploited for highly sensitive detection of glucose.The TMB-HRP-OPD sensor exhibited linear range of 0.1-3.0 μmol/L and 4.0-30 μmol/L respectively for detection of glucose, with limit of detection of 0.02 μmol/L.Furthermore, it was successfully applied to the determination of glucose in real human serum and the results were in good agreement with the clinical data.
2.On-chip magnetic separation of microcantilever immunosensor based on the CdSe QDs-tagged magnetic microbead.
Peng GAO ; Suying YAO ; Bingbo ZHANG ; Ermao LI ; Jin CHANG
Chinese Journal of Biotechnology 2008;24(2):315-322
We designed a novel microcantilever immuosensor based on magnetic microbead, applying different-sized CdSe QDs as fluorescent probes and polystyrene magnetic microbead. The novel microcantilever immuosensor used fluorescent probes embedded polystyrene microbeads and specific antibodies on the surface of the polystyrene microbead. In addition, we studied the mechanism of the on-chip magnetic separation, the structure of micro-electromagnet and the microbead magnetization by the micro-magnetic field, the snake-shaped planar micro-electromagnet for the novel microcantilever immuosensor.
Antibodies
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analysis
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Biosensing Techniques
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instrumentation
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methods
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Cadmium Compounds
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chemistry
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Equipment Design
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Immunoassay
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instrumentation
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methods
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Magnetics
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Micro-Electrical-Mechanical Systems
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instrumentation
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Microspheres
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Nanotechnology
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Polystyrenes
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chemistry
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Quantum Dots
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Selenium Compounds
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chemistry
3. The baseline characteristics of School-based Cardiovascular and Bone Health Promotion Program in Beijing
Dongqing HOU ; Aiyu GAO ; Zhongxin ZHU ; Zhaocang YU ; Ningxiang LIN ; Junting LIU ; Xiaoyuan ZHAO ; Guimin HUANG ; Suying CHANG ; Jie MI
Chinese Journal of Preventive Medicine 2018;52(11):1117-1123
Objective:
To describe the baseline characteristics of School-based Cardiovascular and Bone Health Promotion Program(SCVBH) in Beijing.
Methods:
Children and adolescents were selected in 30 schools (8 primary schools, 21 middle schools and one 12-year education school) from Dongcheng, Tongzhou, Fangshan and Miyun districts of Beijing by using a stratified cluster sampling method. 15 391 students in grade 1 to 4 from primary schools, grade 1 from junior and senior high schools were enrolled in the investigation with an exclusion of students who were not able to participate due to trauma or other uncomfortable physical conditions. The baseline survey including a questionnaire survey, physical examination and blood biochemical test was conducted from September 2017 to January 2018. Obesity, blood pressure, fasting blood glucose, lipid, bone density and grip were evaluated.
Results:
The prevalence of obesity, hypertension, hyperglycemia (impaired fasting glucose and diabetes), dyslipidemia, lower bone density were 22.3%(3 394), 14.8%(2 248), 10.4%(1 490), 20.3%(2 919) and 2.1%(316) in total respectively, and 27.2%(2 081), 16.3%(1 244), 12.8%(922), 22.2%(1 595) and 1.2%(94) for boys, 17.3%(1 313), 13.3% (1 004), 7.9%(568), 18.4%(1 324) and 2.9%(229) for girls. Boys had higher prevalence of obesity, hypertension, hyperglycemia, and dyslipidemia and lower prevalence of low bone density than girls (all
4. Characteristics of cardiovascular health of children and adolescents aged 6-16 years in Beijing during 2017-2018
Fangfang CHEN ; Suying CHANG ; Dongqing HOU ; Aiyu GAO ; Zhongxin ZHU ; Zhaocang YU ; Ningxiang LIN ; Jie MI
Chinese Journal of Preventive Medicine 2018;52(11):1124-1129
Objective:
To describe the status of cardiovascular health (CVH) of children and adolescents aged 6-16 years in Beijing during 2017-2018.
Methods:
The school-based Cardiovascular and Bone Health Promotion Program(SCVBH) was conducted from November 2017 to January 2018. 15 391 children and adolescents aged 6-16 years were selected from grade 1 to 4 in 8 primary schools, grade 1 in 21 middle schools and senior grade 1 in one twelve-year education school in Dongcheng, Fangshan, Miyun and Haidian Districts of Beijing by using a cluster sampling method. Seven cardiovascular health indicators, including smoking, body mass index, dietary intake, physical activity, blood pressure, blood glucose, and total cholesterol, were categorized as poor, intermediate, and ideal according to American Heart Association (AHA) criteria. The status of CVH was evaluated according to the criteria of the ideal CVH related behaviors and factors defined by AHA.
Results:
The proportion of children and adolescents with an ideal smoking, dietary intake and physical activity indicators reached 99.2%(4 982/15 108), 19.7%(2 921/14 805), 17.8% (2 499/14 056), respectively. The proportion of children and adolescents with 7 and 4-5 ideal cardiovascular health indicators was 1.7%(209/12 560) and 65.1%(8 176/12 560). The proportion of children and adolescents with 4-7 ideal cardiovascular health indicators, 2-4 ideal health behavior indicators and 2-3 ideal health factors was significantly higher in urban than that in rural all
5. The analysis of the association of sleep with high blood pressure among children and adolescents aged 6-16 years in Beijing
Guimin HUANG ; Dongqing HOU ; Aiyu GAO ; Zhongxin ZHU ; Zhaocang YU ; Ningxiang LIN ; Suying CHANG ; Jie MI
Chinese Journal of Preventive Medicine 2018;52(11):1136-1139
Objective:
To investigate the sleep status in children and adolescents and explore the relationship between the sleep and high blood pressure in them.
Methods:
Data was from the Schoolbased Cardiovascular and Bone Health Promotiow Program (SCVBH) in Beijing. And 13 471 children and adolescents aged 6-16 years were selected from grades 1 to 4 in 8 primary schools, grade 1 in 21 middle schools and senior grade 1 in one twelve-year education school in Dongcheng, Fangshan, Miyun and Tongzhou Districts of Beijing during 2017-2018 by using a cluster sampling method. Self-reported questionnaires were used to collect the demographic characteristics, lifestyle habits of children and adolescents. The blood pressure was measured by electronic sphygmomanometer. Multiple logistic regression model was used to analyze the association between sleep and hypertension risk.
Results:
The sleep duration was (8.4±1.1) hours. The prevalence of high blood pressure was 15.0%(2 021/13 471). The incidence of mouth breathing, slobber, snoring, and suffocate was 28.4%(3 823/13 471), 23.6%(3 184/13 471) and 18.7%(2 513/13 471), 2.8%(378/13 471) respectively. After adjusting for age, gender, snoring, mouth breaking, slobber, suffocate, the time for bed and sleep duration, we found that snoring and sleep duration (5.0-6.9 h) were both associated with high blood pressure (
6. The evaluation of dietary salt and potassium intake assessed by two 24-hour urine specimens among 284 primary and secondary school students
Jie DONG ; Hui FAN ; Xiaoyuan ZHAO ; Suying CHANG ; Jie MI
Chinese Journal of Preventive Medicine 2019;53(2):185-190
Objective:
To estimate the dietary salt and potassium intake by two 24 hour urine salt and potassium excretion in primary and secondary school students.
Methods:
The boarders from Grade 5 to 6 in a rural primary school and Grade 1 to 3 in a rural middle school in Changde City, Hunan Province were recruited from October to December in 2017. Subjects who had 2 and more urinary incontinence episodes or a history of kidney disease or diarrhea and girls in menstruation were excluded. The final analysis included 284 subjects. Questionnaires on basic demographic information were collected, and height and weight were examined. Two inconsecutive 24-hour urine specimens were collected in weekdays. The differences and consistency between two 24-hour urine were tested and dietary salt and potassium intake were assessed by the average of two 24-hour urine collections.
Results:
A Total of 284 participants was (12.1±1.5) years old, of which 148 (52.1%) were boys and 36 (12.7%) were overweight. In the first 24-hour urine, the volume [
7. The association between body fat distribution and calcaneal bone mineral density in children and adolescents aged 6-16 years in Beijing
Haibo LI ; Dongqing HOU ; Junting LIU ; Aiyu GAO ; Zhongxin ZHU ; Zhaocang YU ; Ningxiang LIN ; Suying CHANG ; Jie MI
Chinese Journal of Preventive Medicine 2019;53(2):191-195
Objective:
To understand the association between body fat distribution and calcaneal bone mineral density (BMD) in children and adolescents aged 6-16 years in Beijing.
Methods:
Children and adolescents were selected in 30 schools (8 primary schools, 21 middle schools and one 12-year education school) from Dongcheng, Tongzhou, Fangshan and Miyun districts of Beijing by using a stratified cluster sampling method from November 2017 to January 2018. A total of 15 030 students in grade 1 to 4 of primary school, grade 1 of junior and senior middle school were enrolled after excluding subjects who were not able to participate into this study due to trauma or other uncomfortable physical conditions or with missing key values or with diabetes and kidney diseases. Questionnaire survey, body composition and calcaneus bone mineral density were conducted. The relation of fat mass percentage (FMP), trunk to total fat ratio (TrTFR), trunk to limb fat ratio (TrLFR), limb to total fat ratio (LTFR) and viscera to total fat ratio (VTFR) with calcaneus BMD were assessed using the multivariate linear regression models after adjusting for possible confounding effects. The central fat distribution types were divided into 4 groups including type 1: both trunk fat and visceral fat greater than the sex-and age-specific internal
8. The association between blood lipids and calcaneus bone mineral density in children and adolescents aged 6-16 years in Beijing
Pei XIAO ; Dongqing HOU ; Aiyu GAO ; Zhongxin ZHU ; Zhaocang YU ; Ningxiang LIN ; Junting LIU ; Suying CHANG ; Jie MI
Chinese Journal of Preventive Medicine 2019;53(2):196-201
Objective:
To investigate the association between blood lipid and calcaneus bone mineral density (BMD) in children and adolescents aged 6-16 years in Beijing.
Methods:
Children and adolescents were selected in 30 schools (8 primary schools, 21 middle schools and one 12-year education school) from Dongcheng, Tongzhou, Fangshan and Miyun districts of Beijing by using a stratified cluster sampling method from November 2017 to January 2018. A total of 14 303 students in grade 1 to 4 of primary school, grade 1 of junior and senior middle school were enrolled after excluding subjects who were not able to participate into this study due to trauma or other uncomfortable physical conditions or with missing key values or with diabetes and kidney diseases. Questionnaire survey, blood lipid and calcaneus BMD were conducted. Multivariate linear regression was applied to quantify the association between calcaneal BMD as a dependent variable and blood lipid level as an independent variable after adjusting for the potential confounding factors. Furthermore, quantile regression was used to analyze the association between blood lipid level and different percentiles (