OBJECTIVE To monitor the distrubution and the change in drug resistance of pathogenic bacteria from lower respiratory tract in patients with comprehensive intensive care to use the antibiotic properly in the clinics.METHODS All lower respiratory infection cases admited to the ICU in 2005-2006 were reviewed,and the distribution of pathogenic bactieria and their drug resistance profile were analyzed.RESULTS 89.3% pathogenic bacteria were Gram-negative ones,among them Pseudomonas aeruginosa(47.6%) and Klebsiella pneumoniae(21.2%) were prevailed.Staphylococcus aureus(6.2%) was the most prominent Gram-positive bacteria,MRSA showed increasing trend.Fungi accounted for 5.8%.All pathogenic bacteria showed high resistance to the antibiotics.CONCLUSIONS The pathogenic bacteria and their resistance to the antibiotics are highly changed at ICU.Intensive observations from the clinicians are recommanded so as to make the antibiotics effective.

Based on the half sphere-cylinder myocardial model, a quantitative calculating method of gated SPECT myocardial infraction of the left ventricular functional parameters is proposed. To overcome the shortage of the ellipse model and other normal methods, this method uses the half sphere-cylinder myocardial model and its subarea. The experiment result shows that the data from normal men accord very well with those reported in domestic and international literature. The result from use in the case of left ventricular myocardial infarction is also satisfactory. Therefore, this method is of high value clinically.
Coronary Artery Disease ; diagnostic imaging ; physiopathology ; Gated Blood-Pool Imaging ; methods ; Heart Ventricles ; diagnostic imaging ; Humans ; Image Interpretation, Computer-Assisted ; Male ; Models, Cardiovascular ; Technetium Tc 99m Sestamibi ; Tomography, Emission-Computed, Single-Photon ; methods ; Ventricular Function, Left

ObjectiveTo explore the possible mechanism of action of Lifei Xiaoji Pill （理肺消积丸， LXP） in the treatment of non small cell lung cancer based on the Warburg effect and the USP47/BACH1 pathway. MethodsFifty C57BL/6 mice were randomly divided into five groups， model group， LXP group， inhibitor group， LXP + inhibitor group， and cisplatin group， with 10 mice in each group. A lung cancer mouse model was established by subcutaneously injecting Lewis cells. On the next day， the model group mice were given 0.2 ml of saline by gavage daily， the LXP group given 240 mg/（kg·d） of LXP solution once a day by gavage， the inhibitor group intraperitoneally injected with P22077 at a dose of 10 mg/（kg·d） every day， the LXP + inhibitor group given both LXP by gavage and P22077 by intraperitoneal injection once a day， and the cisplatin group received 0.5 mg/（kg·d） cisplatin intraperitoneally every other day. All treatments lasted for 14 days. On the day after the last dose， tumor weight and volume were measured， tumor histopathology was examined by HE staining， apoptosis in tumor tissues was detected by TUNEL staining， and proliferation cell nuclear antigen （PCNA） protein levels were detected by immunohistochemistry. Warburg effect indicators， including glucose concentration， lactate content， and adenosine triphosphate （ATP） production in tumor tissues， were measured. Western Blot and qRT-PCR were used to detect the protein and mRNA expression levels of USP47， BACH1， hexokinase 2 （HK2）， and glyceraldehyde 3-phosphate dehydrogenase （GAPDH）. ResultsCompared with the model group， all drug intervention groups showed reduced tumor weight and volume， improved tumor pathology， decreased PCNA positive rate， increased apoptosis rate， and reduced expression levels of USP47， BACH1， and HK2 proteins and mRNA （P＜0.05 or P＜0.01）. Except for lactate content in the cisplatin group， the glucose concentration in tumor tissues of other drug intervention groups increased， while lactate content and ATP production decreased （P＜0.05 or P＜0.01）. Compared with the LXP group， the LXP + inhibitor group showed more significant improvements in these indicators （P＜0.05 or P＜0.01）. Compared with the cisplatin group， the LXP + inhibitor group had lower mRNA expression of HK2 and GAPDH， and lower protein levels of USP47 and HK2 （P＜0.05 or P＜0.01）. Compared with the inhibitor group， the cisplatin group had higher HK2 protein levels， while the LXP + inhibitor group showed lower mRNA expression of BACH1， HK2， and GAPDH （P＜0.05 or P＜0.01）. ConclusionLXP significantly inhibits tumor growth in lung cancer mice， and its mechanism of action may be related to inhibiting the Warburg effect via the USP47/BACH1 pathway.