Objective To observe the changes of dopamine and its metabolites,melatonin,as well as melatonin receptors in the substantia nigras (SN) in 6-OHDA induced hemiparkinsonian rats.Methods PD rat was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta and medial forebrain bundle.Rats were divided into PD groups and vehicle groups,and sacrificed at 10:00 and 22:00 respectively,at 3 and 6 weeks post-lesion.The contents of dopamine and its metabolites as well as melatonin were measured by HPLC.The expressions of MT1 and MT2 mRNA and protein were detected by RT-PCR and Western-Blot respectively.Results Dopamine levels declined by 83% and 88% respectively at 3 and 6 weeks post-lesion in the injected side of PD rats,compared with the contralateral side.Melatonin levels in the injected side increased by 22% and 29% respectively.However,melatonin increased by 46% and 31% respectively at 10:00 and 22:00,at 6 weeks post-lesion in the contralateral side,compared with the same side of the vehicle.The expressions of MT1 and MT2 receptors mRNA were significantly decreased by 91.04% and 90.24% respectively in comparison with the vehicle,while the protein expression of MT1 and MT2 receptors significantly declined by 58% and 70% respectively.Conclusion Increased melatonin and down-regulated MT1 and MT2 receptors in the lesioned SN in 6-OHDA-induced rats may be associated with the progression of PD.