Objective To study the effect of different negative pressure values of negative pressure wound therapy (NPWT) effect on skin graft .Methods Eighty patients were selected ,who underwent chronic wound bed and preparedto treat with skin graft in surgical ward of emergency department in the Second Affiliated Hospital of Chongqing Medical University from January 2012 to July 2012 .Eighty patients were divided into 8 groups ,group A were treated with packing pressure after skin graft ,group B~ H were treated with continuous NPWT ,pressures were -10 ,-20 ,-30 ,-40 ,-50 ,-60 and -70 kPa ,respectively .Whether they were in comfortable situation at second day after surgery were observed .Flap survival area at Seventh day after surgery was meas‐ured .Results The patient comfort ,flap survival area ,wound healing time of group B to H were higher than those of group A (P<0 .05) .Patient comfort tended to decrease with the increasing negative pressure values between -10 - -40 kPa(P<0 .05) .Flap survival area ,healing time of group B ,C were higher than those of group D to F(P<0 .05) .Conclusion Application of NPWT technology in wound ,-10 kPa negative pressure value can not only improve wound blood circulation ,but also ensure patient be comfortable .

Objective] Discussing the relationship between COPD phenotypes and patterns of TCM. [Methods] Discussing the relationship by searching of documents about research of COPD phenotypes. [Result]The phenotype of chronicbronchitis and exacerbation, emphysema,inflammation,FEV1 declining or overlap syndrome may match with different patterns of TCM.[Conclusion] COPD phenotypes have similarities with patterns of TCM at component, influence and typing target.

Objective To investigate the application effect of scene exercise training model of 6 emergency disease entities in the junior nurses training .Methods Thirty‐six newly employed nurses during 2010-2013 were performed the scene exercise train‐ing of six disease entities .Before and after training ,the the forms of questionnaire investigation ,written examination and implemen‐tation were adopted to survey and analyze the post competence ,training attitude ,theoretical knowledge ,operation skill ,single time for help and participating doctor′s satisfaction on the coordinate ability .Results After training ,the aspects of post competence , training attitude ,theoretical knowledge ,operation skills ,single time for help and participating doctor′s satisfaction on the coordinate ability in junior nurse were significantly improved ,the differences between before and after training had statistical significance (P<0 .01 or P<0 .05) .Conclusion Using the scene exercise training model of 6 emergency disease entities in junior nurses training has active significance for increasing the overall quality of junior nurses .

Objective To investigate the size and diversification of peripheral nerve measured by high frequency ultrasound in di-abetic peripheral neuropathy patient .Methods The endings of the medial branch of deep peripheral nerves(DPN) were interrogated by high frequency ultrasound and nerve electrophysiology ,and the nerve conduction characters were studied in a cohort of 150 clini-cally diagnosed diabetic peripheral neuropathy patients and a control group of 100 healthy volunteers .At the sametime these cases underwent electrophysiological examination .Results Distinct echoic appearances were consistently detected between the DPN nerves of diabetic peripheral neuropathy patients and healthy volunteers .The sensitivity rate of high frequency ultrasound and nerve electrophysiological examination in diagnosis of diabetic peripheral neuropathy were 91 .33% and 74 .67% ,and the specificity rate were 91% and 82% .Conclusion High frequency ultrasound may play a role in the diagnosis of diabetic peripheral neuropathy .

Objective To explore the effect of core competence oriented advanced course in wound ostomy specialist nurse training.Methods A total of 101 Chongqing municipal wound ostomy specialist nurse training students from September 2016 to June 2017 were selected.Among them,50 students in 2016 served as the control group and trained by traditional training methods;51 students in 2017 served as the observation group and adopted the core competence oriented advanced courses for conducting the training.Then the effects after training were compared between the two groups.Results The core competence score after training in the observation group [(3.11±0.11) point]was higher than that in the control group [(2.87±0.13) point],the difference was statistically significant (P＜0.05).Conclusion The core competency oriented advanced course is effective for training the wound ostomy nurses.