Objective:To study the effects of osteoid hydroxyapatite(OHA)in repair of bone defect.Methods:Bone defect in the size of 9 mm?5 mm?4 mm was made in bilateral thigh-bone entocondyle in 12 rabbits,OHA was implanted in the defects on one side and hydroxyapatite(HA)on another side.2,4,8 and 12 weeks after operation 4 rabbits were killed respectively,bone samples with the implants were prepared and examined by roentgenography,histology and SEM.New bone formation was determined by measuring the area of new bone in the samples.Results:Gross imspection and roentgenography observation showed that the defects were repaired in both groups 12 weeks after operation.Histological examination showed that new bone was increasing,absorption and disintegration of the implants were progressing from 2 to 12 weeks after operation.4,8 and 12 weeks after operation larger new bone area was found in OHA implanted defects than that in HA implanted(P

ObjectiveTo analyze the therapeutic experience and value of hemoperfusion in children with acute tetramine poisoning.MethodsAccording to the choice of children's legal guardian,67 cases with acute tetramine poisoning were divided into two groups:the perfusion group (48 cases),and the control group (19 cases).The patients in perfusion group received blood perfusion based on conventional treatment,while the patients in control group received conventional treatment only.Survival outcomes of patients with different serum tetramine concentrations in two groups were compared.ResultsWhen the serum concentration of tetramine was ＞0.5 mg/dl,patients in both groups died in a short time.When the serum concentration of tetramine was 0.20～0.49 mg/dl,the drop value of serum concentration of tetramine significantly greater in perfusion group [(0.28±0.02) mg/dl] than that in control group [(0.13±0.03) mg/dl] (P＜0.05).When the serum concentration of tetramine was ＜0.2 mg/dl,the drop value of serum concentration of tetramine in two groups showed no significant difference [ (0.12±0.02) mg/dl vs (0.11±0.03) mg/dl] (P＞0.05).Conclusion The treatment method for tetramine poisoning should be selected based on the serum concentration of tetramine.

Objective:To explore the vulnerability and related risk behaviors of the out-of-school adolescents floating with their parents from country to city.Methods:From September to October in 2005,260 out-of-school adolescents who were 14 to 20 years old were investigated in our study.Results:The out-of-school adolescents lacked of HIV/AIDS knowledge and life skills,and their psychosocial competence was at a lower level.The adolescents were open-minded to sex,but they were not conscious of using condoms.Attitude towards people with HIV was positively correlated with a well-being family,harmonious relation between their parents,a stable job,knowledge,life skills and psychosocial competence(such as in control of emotions,empathy).Their life skills were positively correlated with their mothers'schooling satisfaction with family income,a stable job,high scores of knowledge,social responsibility,and interpersonal relationship.Competence of HIV/AIDS prevention was positively correlated with schooling,a stable job,knowledge,life skills,and psychosocial competence.Conclusion:Out-of-school adolescents were vulnerable to HIV/AIDS.Individual level intervention combined with structural intervention could effectively reduce their vulnerability.Effective and feasible health education approach to enhance their life skills and psychosocial competence was a key point to health education on HIV/AIDS prevention.

AIM: To evaluate the efficiency of buflomedil hydrochloride injection (Buf) in the treatment of the damage of peripheral nerve induced by diabetes (DPN). METHODS: 28 patients with DPN were given Buf (150 mg?d -1 , iv gtt) and another 28 patients were used routine therapy. The treatments lasted for 15 d. RESULTS: The total efficiency rate was 96.5 % in Buf group, while it was 57.1 % in routine therapy group (P

Objective:To explore the effect of positive psychological intervention based on PERMA model on the fear of cancer recurrence and psychological capital in breast cancer patients during chemotherapy.Methods:A total of 60 breast cancer patients who received chemotherapy from November 2019 to January 2020 were divided into intervention group and control group with 30 cases in each group. The control group received routine health education. On this basis, the intervention group was given 8 times of positive psychological intervention. The general situation questionnaire, Fear of Progression Questionnaire-Short Form (FoP-Q-SF) and Positive Psychological Capital Questionnaire (PPQ) were used to evaluate the results of the two groups before and after intervention.Results:Before, 4 weeks after and 8 weeks after the intervention, the score of fear of cancer recurrence in the intervention group were 39.67±4.24, 34.60±3.78 and 31.17±3.60. The difference of the score of fear of cancer recurrence at different time points was statistically significant ( F value was 257.757, P<0.05). The scores of psychological capital were 113.20±19.75, 125.57±18.79 and 134.43±17.59. The difference of psychological capital level at different time points was statistically significant ( F value was 154.225, P<0.05). Conclusions:Positive psychological intervention based on PERMA model can reduce the fear of cancer recurrence in breast cancer patients during chemotherapy and improve their psychological capital level.

OBJECTIVE To discuss the available methods of preventing the mattress pollution in the wards of the(hospital), and to keep the mattresses clean and dry and eliminate the hidden danger of nosocomial infection.(METHODS) The mattress protective covering is used of a new type of textile materials,and then applied into clinic.Selected 100 pieces of mattresses in the wards,divided them equally into two groups at random,the(experimental) group and the control group.After cleaning and pasteurization(surface) sampling and bacterial culturing for every mattress were undertaken.For the experimental group,spread the protective(covering) before the sheet,and for the control group,used the sheet directly.The colony number of each group was compared in the 3rd,7th,and 14th days.RESULTS The mattresses of experimental group were clean,light polluted and with less colonies,and that of the control group were heavy polluted and with more colonies.The comparisons of the total colony number and the number of every sampling point in the 3rd,7th,and 14th days of the two groups showed that there was a(significant) difference(P

Objective To investigate the expression and significance of microRNA-195 (miR-195) and Bcl-2 in different type of childhood acute lymphoblastic leukemia (ALL) cells.Methods A total of 110 patients diagnosed as ALL from the Pediatrics Department and Outpatient Department of the First Affiliated Hospital of Zhengzhou University from July 2010 to December 2016 were recruited.The patients included newly diagnosed group,remission group and relapse group in which the newly diagnosed group was divided into standard risk(SR) group,intermediate risk (IR) group and high risk (HR) group.And another 14 gender-and age-matched children received bone orthopaedic surgery who excluded tumor and blood system diseases in the same hospital were recruited as control group during the same period.Bone marrow samples of 2-5 mL were drawn from all the subjects.The samples of miR-195 and the expression of Bcl-2 mRNA were detected by using quantitative real time polymerase chain reaction,while the expression of Bcl-2 protein was also detected by using Western blot.Results MiR-195 expression was lower in the newly diagnosis and relapse ALL children (SR:1.25 ± 0.15;IR:1.63 ± 0.54;HR:0.78 ± 0.35;relapse:0.46 ± 0.18),compared with the control group,and there were statistically significant differences (t =42.44,28.63,41.35,42.65,all P =0.000),but the expression of Bcl-2 mRNA was higher in the newly diagnosis and relapse ALL children than that in the control group (SR:2.56 ± 0.91;IR:4.52 ± 0.43;HR:5.49 ± 1.28;relapse:5.32 ± 1.15),which had statistically significant differences (t =3.58,9.86,10.12,8.81,all P =0.000).With the rising of the criticality of ALL,the expression of miR-195 reduced gradually,and the expression of Bcl-2 mRNA increased step by step.Determining the protein of Bcl-2 detected by Western blot showed the same result.Conclusions The lower expression of miR-195 and the higher expression of Bc1-2 may be a marker to distinguish the risk factors of ALL,which may be important for the diagnosis and the determination of the degree of risk for the ALL children.

The student majoring in Medical Biological Technique will be mainly engaged in the practical work of biological technique industry after graduation in the future.In order to bind the theories on the practical biological techniques in designs of contents and roundly improve students' practical ability in classes as well as enriching the communication among students,our college offers an immunologic techniques experiment classes with 36 hours per semester,which has also undergone a reasonable project teaching innovation. This proved to result in a satisfactory outcome in improving the students' practical a

Objective To observe the fundus imaging characteristics of different stages of syphilitic posterior uveitis. Methods Retrospective cases series. Forty-six eyes of 32 patients with syphilitic posterior uveitis were included. There were 14 patients (16 eyes) and 18 patients (30 eyes) were assigned to acute stage group (with the course<2 months) and chronic stage group (with the course≥2 months) respectively. All eyes received the examination of indirect ophthalmoscopy, color fundus photography, fundus fluorescein angiography (FFA) and optical coherence tomography (OCT). All patients received regular anti-syphilitic treatment. Color fundus photography and OCT were followed after treatment. The fundus imaging characteristics of different stages of syphilitic posterior uveitis were observed. Results Indirect ophthalmoscopy and fundus color photography showed that in the acute stage group, there were 3/16 eyes with optic disc edema;4/16 eyes with a yellowish, placoid lesion involving the macular. There were only some pigment alterations on the fundus after treatment. In the chronic stage group, there were 4/30 eyes with optic disc hyperemia, 3/30 eyes with cystoid macular edema. After treatment, the optic hyperemia vanished gradually, but there were still some pigment alterations. The FFA images of two groups showed various vascular leakages. In the chronic stage group, patients also showed hyper-fluorescence with cystoid macular edema. The patients with course 2–3 years have more transmitted fluorescence on FFA. OCT showed that all eyes in the acute stage group had lost the ellipsoid zone, with irregular granular reflectivity of the retinal pigment epithelium (RPE) layer, 6 eyes with subretinal fluid in the macular. After treatment, the ellipsoid zone and RPE layer structure recovered gradually. In the chronic stage group, all eyes showed widespread loss of the ellipsoid zone, pigment migration and (or) cystoid macular edema. After treatment, the ellipsoid zone showed partial recovery. The outer ellipsoid zone was still discontinuous in patients with long duration. Conclusions Syphilitic posterior uveitis patients generally had normal fundus, but some cases had a yellowish, placoid lesion involving the macular. FFA showed various vascular leakages, and the chronic stage group showed more transmitted fluorescence. The major OCT change was loss of the ellipsoid zone or with subretinal fluid. After treatment, fundus showed no abnormal manifestations except some pigment alterations;the ellipsoid zone structure recovered gradually in acute stage eyes, partially recovered in chronic stage eyes.

Objective To determine whether the cross-sectional area (CSA)of the calf measured with MRI could stage lower ex-tremity lymphedema (LEL)secondary to gynecological cancer treatments.Methods 148 patients were enrolled in this research.116 females with unilateral LEL and 32 without LEL after gynecological cancer treatments underwent calf MRI and water displacement. Total soft tissue CSA (T),muscle CSA (M)and subcutaneous tissue CSA (S)of affected calf,and difference of T (DT),M (DM) and S (DS)between calves were obtained on MRI at mid-calf level.Volume of affected calf and difference of volume (DV)between calves were obtained by water displacement.Statistical analysis was performed to determine feasibility of MRI measurements for ac-cessing LEL.Results There were close correlations between volume and T or S of affected calf,and between DV and DT or DS of calves.The correlations of stages of LEL with T and S of affected calf as well as DT and DS were stronger than the volume of affect-ed calf and DV (P< 0.01).Multivariate analysis showed more significant differences in T and S than in volume of affected calf,and in DS than in DV between LEL stages (P< 0.05).No difference was found in volume of affected calf and in DV between stage 0 andⅠ. For staging LEL,DS showed the most profound discrimination ability among all measurable parameters.Conclusion DS of calves could be the most reliable parameter recommended for staging and early diagnosis of LEL.