Objective To observe the efficacy of the bivalent of clostridium butyricum powder in the treat-ment of childhood diarrhea after pneumonia .Methods 82 cases of patients with childhood diarrhea after pneumonia in our hospital were divided into two groups according to hospital sequence .42 cases of the control group were given symptomatic treatment with smecta and the 40 cases of the study group used bivalent of clostridium butyricum powder in basis of the control group .The diarrhea treatment time and length of stay ,the efficacy of the treatment were com-pared between the two groups .Results The total effective rate of the study group was 95.0% significantly higher than 71.4%of the control group,the difference was statistically significant (χ2 =8.05,P<0.05).The study group′s duration of diarrhea were (2.3 ±1.1)d,pneumonia duration of treatment were (4.4 ±1.9)d,length of hospital stay were(4.5 ±2.1)d significantly lower than those of the control group ,the differences were statistically significant (t=7.39,7.35,7.39,all P<0.05).Conclusion The bivalent of clostridium butyricum powder has a significant effect in the treatment of childhood diarrhea after pneumonia , which is worth to be applied in clinical application .

Thyroglobulin (Tg) and radioiodine whole body scan (WBS) have been commonly used in follow-up of patients with differentiated thyroid carcinoma (DTC). Tg is associated with radioiodine uptake in local or distant metastases. In minority of patients, the follow-up scan shows no functioning thyroid tissue, but the serum thyroglobulin is still elevated. Therefore, we review recent developments of diagnosis and treatment of those patients with differentiated thyroid cancer and with thyroglobulin elevation but negative iodine scintigraphy.
Humans ; Iodine Radioisotopes ; Radionuclide Imaging ; Sensitivity and Specificity ; Thyroglobulin ; blood ; Thyroid Neoplasms ; diagnostic imaging ; therapy

Objective To evaluate the value of speckle tracking imaging( STI) by investigating the correlation and consistency between two?dimensional STI and 99 Tcm?MIBI G?MPI in the assessment of myo?cardial injury and myocardial infarction ( MI) of swine model. Methods Eight healthy and same?age adult female Tibetan swine were used to establish animal models of MI induced by open?chest ligation of the first diagonal branch of the left anterior descending coronary artery. The swine underwent ultrasonic cardiogram and G?MPI before and at 6, 24, 48, 72 and 96 h after the operation. The myocardial perfusion was evalua?ted. Ultrasonic sectional graphs were collected to analyze ultrasonic short?axis horizontal peak values of sys?tolic radial strain ( SR) and systolic circumferential strain ( SC) and local myocardial rotation angle ( Rot) of different segments of the left ventricle with off?line STI. Left ventricular ( LV) EDV, LVESV, LVEF by G?MPI and ultrasonography were compared. Pearson correlation analysis and repeated measures analysis of variance were used. Results Six models were successfully constructed. Six hours after the coronary artery ligation, the G?MPI showed no significant or very few perfusion defects;24, 48, 72 and 96 h after the liga?tion, G?MPI showed sparseness and defects at the anterior septum, anterior and lateral wall of apical seg?ment of apical segment. The peak strain values of SR, SC and Rot at the anterior septal apical segment, the anterior and lateral wall of apical segment decreased at different time points after the ligation. Six hours after the coronary artery ligation, the STI was positive. For healthy swine, the LVEF measured with G?MPI and ultrasonic STI showed good correlation ((68±3)% vs (70±4)%, r=0.84, P<0.05). The LVEF values of G?MPI and ultrasonic STI at 6, 24, 48, 72, 96 h after the ligation were (79±1)%, (80±4)%, (58±2)%, (54±4)%, (57±4)%, and(76±2)%,(76±3)%,(57±3)%,(55±3)%,(68±6)% (r:0.40-0?76, all P<0.05). LVEDV and LVESV measured by the two methods showed variation trends at different time points ( F=64.104, 30?069, 35.186, 54.903, all P<0.01) with good consistency after the ligation. Conclusions Ultrasonic STI could estimate the myocardial injury at an earlier stage and measure the severity of damaged myocardium quantitatively, and the resluts are in good agreement with myocardial perfusion and function by G?MPI.

Objective To investigate the bilateral frontal cerebral contusion complicated by severe central herniation early treatment .Methods 54 cases of severe bilateral frontal cerebral contusion complicated by central herniation patients were randomly divided into the observation group and control group using number table .The obser-vation group were taken the early surgical treatment and the control group were not underwent surgery .The clinical symptoms and prognosis of treatment were compared .Results After treatment ,the GCS score of the observation group was (12.98 ±3.85),which was higher than (11.35 ±4.12) of the control group(t=2.354,P<0.05),and the total time of the observation group ICU hospitalization time was significantly lower than the control group (t=10.007,P<0.01;t=19.278,P<0.01);The good recovery rate of the observation group (40.74%) was higher than the control group(14.81%)(χ2 =4.523,P<0.05),the residual rate of the observation group ,severe disability rate,plant sur-vival and mortality (33.33%,14.81%,7.41%,3.70%) were lower than that of the control group (44.44%, 22.22%,11.11%,7.41%),but the difference was not significant between the two groups (χ2 =2.587,2.135, 2.268,2.189,all P>0.05).Conclusion Dual frontal severe brain injury complicated by central herniation patients with early diagnosis and surgical treatment method using craniotomy decompression can significantly improve the cure rate and effectively improve the prognosis of patients with treatment .

Lung metastases are more common in metastatic disease in differentiated thyroid carcinoma (DTC). Because of its insidious onset and slow development, clinical diagnosis is relatively difficult. Some possible diagnostic methods for detecting the lung metastasis of DTC including serological examination, radionuclide imaging and other medical imaging patterns are discussed in this paper. The progress and the current situation about investigation of those modalities which are in the early diagnosis, recurrent and clinical evaluation for the lung metastasis of DTC are briefly reviewed. Therefore, it is expected to promote DTC with lung metastasis to a higher diagnostic level.
Humans ; Lung Neoplasms ; diagnosis ; secondary ; Neoplasm Metastasis ; Thyroid Neoplasms ; pathology

Objective To evaluate the influence of SP600125,a selective c-Jun N-terminal kinase (JNK) inhibitor,on the levels of serum total bile salt (TBA) and Bsep,Ntcp expression in the hepatic tissue of rats with ethinylestradiol induced intrahepatic cholestasis.Methods Rats pregnant for 15days were administered the subcutaneous injection of 17- b -estradiol propylene ( EE ) to modulate the ICP animal models,and be SP600125 to intervene.Testing the level of serum TBA and the expression of c-Jun,Bsep,Ntcp in the hepalatic tissue.Results The average gray values of c-Jun in the group of ICP models were significantly lower than the normal control group ( 101.05 ± 5.20 vs 118.99 ± 5.95,P ＜ 0.05 ).After the intervention of SP600125,comparing with the group of ICP models,the expression of Bsep,Ntcp in the group of SP600125 intervention were significantly higher,and this change in the high dose of SP600125 intervention group was more obvious ( low dose intervention group Bsep:0.452 ±0.031 vs 0.291 ±0.043,Ntcp:0.462 ± 0.015 vs 0.285 ± 0.021,P ＜ 0.05 ; high dose intervention group Bsep:0.568 ± 0.038 vs 0.291 ±0.043,Ntcp:0.605±0.020 vs 0.285 ±0.021,P ＜0.05),while the level of TBA in the serum was significantly lower.Conclusions Treatment with SP600125 can down-regulate the level of c-Jun/AP-1,and it may participate in the lower expression of Bsep、Ntcp in the ICP rats which were induced by 17-bestradiol.

Objective To investigate the expression and distribution of Kv1.6 in rat hippocampus after status epilepticus (SE) induced by lithium and pilocarpine to explore the relationship between Kv1.6 and epileptogenesis.Methods Forty-eight healthy male Sprague-Dawley rats were randomly divided into experiment group and control group.The rats in each group were randomly assigned into 6-hour,1-day,2-day and 3-day subgroups (n =6).Electroencephalography (EEG) of rats in both groups were recorded to show the electrical activity of the rat brains.Nissel staining was performed to investigate the pathological changes,and immunohistochemistry and Western blotting were applied to analyze the expression and distribution of Kv1.6.Results (1) The EEG of rats in control group showed α wave with uniform amplitude,while the EEG in rats subjected to pilocarpine injection showed slow waves and spike waves with irregular amplitude and rhythm.Sustained spike waves were observed during SE.(2) Nissl staining showed that 6 hours after SE,the neural morphology of hippocampi in experiment group was as the same to that in control group.There was no change in neuronal morphology and numbers.However,1 day after SE,the structure of hippocampi was discrete and the number of neurons was decreased.Two and 3 days after SE,the number of neurons in hippocampi decreased further.Swelling and deformation of neurons were seen.Nissl bodies emerged,decreased or even disappeared.(3) There was no statistically significant difference in the expression of Kv1.6 in CA3 and CA1 areas between experiment group and control group 6 hours after SE (all P＞0.05).One day,2 days and 3 days post SE,the expression of Kv1.6 in CA3 area (0.117 ±0.040,0.135 ±0.029,0.112 ±0.050) decreased obviously compared to control group (0.192±0.054,0.201 ± 0.062,0.184 ±0.042;t---3.861,3.745,5.579,all P ＜0.05);and the expression of Kv1.6 in CA1 area (0.114 ± 0.02,0.082 ± 0.031,0.106 ± 0.043) decreased significantly compared to control group (0.165±0.036,0.187 ±0.025,0.172 ±0.052;t=3.221,6.360,3.645,all P＜0.05).The expression of Kv1.6 did not show statistically significant difference in DG area between experiment group and control group at all time points examined (P ＞ 0.05).The Western blotting showed the same trend.Conclusion The decreased expression of Kv1.6 may play a role in the epileptogenesis.

Objective To study the effect of different prostaglandin E2 (PGE2) combined with tumor necrosis factor-?(TNF-?) to the IP3-Ca2+ pathway mediated by membrane TNF-?R of fibroblasts. Methods The lung fibroblasts of breed mouse were primarily cultured. 10 ?g/L TNF-? and 10 ?g/L TNF-? combined with PGE2 of different doses were added to culture medium of fibroblasts. At some observation time,the expression of TNF-?R on the cellular membrane of fibroblasts was detected by the method of immunohistochemistry,the level of IP3 in cells was detected by the method of radioimmunoassay and the contents of Ca2+ in fibroblasts by the method of flow cytometry. Results With doses of PGE2 increasing at 30 s time point and 60 s time point,the cpm value of IP3 of fibroblast decreased gradually. The cpm value of IP3 significantly increased when the dose of PGE2 was 1.0 ?g/L,but obviously lower when the dose of PGE2 was 2.0 ?g/L at 120 s time point. The expression of TNF-?R and the contents of Ca2+ decreased in a dose-dependent way with the dose of PGE2 increasing. Conclusion Certain dose of PGE2 could suppress the effect that TNF-? enhance the cell proliferation of fibroblast by the IP3-Ca2+ pathway mediated by membrane TNF-?R of fibroblasts.

BACKGROUND:There are many reports on the effects of low-to-moderate intensity endurance exercise on mitochondrial biogenesis in the skeletal muscle, but there is no understanding about the high-intensity endurance exercise. It has not been reported whether the high-intensity endurance exercise influence the mitochondrial biogenesis in the skeletal muscle through 5′-adenosine monophosphate-activated protein kinase (AMPK)/silent information regulator factor 2 related enzyme 1 (SIRT1) signal molecules. OBJECTIVE: To investigate the effect of AMPK/SIRT1 signaling cascade on the mitochondrial biogenesis in the skeletal muscle during different intensities of endurance exercises. METHODS:Forty-two male Sprague-Dawley rats were randomly divided into sedentary group, moderate-intensity exercise group and high-intensity exercise group. Moderate-intensity exercise load was 28 m/min, 60 min/d;high-intensity exercise load was 38 m/min, 60 min/d. In both exercise groups, the rats had 5 days of exercise and 2 days of rest within 1 week, and the exercise lasted for totaly 7 weeks. The animals of exercise groups were kiled immediately, 6 hours and 24 hours after exercise. Quantitative PCR was used to detect peroxisome proliferators γ activated receptor coativator-1α (PGC-1α) and SIRT1 gene expression, and western blot to detect phosphorylated-AMPK (P-AMPK) and SIRT1 protein expression. RESULTS AND CONCLUSION:(1) Immediately, 6 hours, 24 hours after moderate-intensity exercise, PGC-1α mRNA expression in the skeletal muscle was 362% (P < 0.01), 675% (P < 0.01) and 116% of that in the sedentary group, P-AMPK protein expression was 112%, 165% (P < 0.05), 129% (P < 0.05) of that in the sedentary group, the expressions of SIRT1 protein and mRNA were 55% (P < 0.05), 86%, 103% and 109% (P < 0.05), 155%, 132% (P < 0.05) of those in the the sedentary group, respectively. (2) Immediately, 6 hours, 24 hours after high-intensity exercise, PGC-1α mRNA expression in the skeletal muscle was 274% (P < 0.01), 130% (P < 0.05), 68% (P < 0.05) of that in the sedentary group, the expression of P-AMPK protein was 235% (P < 0.01), 166% (P < 0.05), 160% (P < 0.05) of that in the sedentary group, the expressions of SIRT1 protein and mRNA were 199% (P < 0.01), 166% (P < 0.05), 164% (P < 0.05) and 255% (P < 0.01), 292% (P < 0.01), 122% of those in the sedentary group, respectively. These findings indicate that (1) 7 weeks of moderate-intensity endurance exercise significantly increased PGC-1α gene expression in the skeletal muscle, and its mechanism may involve AMPK/SIRT1 signaling cascade; (2) 7 weeks of high-intensity endurance exercise significantly inhibited PGC-1α gene expression at 24 hours after exercise,and this process was carried out in an AMPK/SIRT1 signal cascade independent manner.

Objective To compare the diagnostic values of 18F-fluorodeoxyglucose-single photon emission computed tomography (18F-FDG-SPECT) and helical CT in the detection of the metastasis of postoperative breast cancer.Methods A total of 94 patients with postoperative breast cancer were chosen as research objects.The follow-up duration of post-operation was 2 years.All patients were received by 18F-FDG-SPECT and helical CT during follow-up.Lymph nodes that were suspected to the postoperative metastasis were taken for histological procedure.The diagnostic values of 18F-FDG-SPECT and helical CT to the metastasis of postoperative breast cancer were compared.Results Compared to helical CT,the sensitivity,specificity,positive predictive value,negative predictive value,and positive likelihood ratio of 18F-FDG-SPECT were higher (P ＜ 0.05);however,the negative likelihood ratio of 1s F-FDG-SPECT were lower (P ＜ 0.05).Conclusions 18F-FDG-SPECT has more important clinical value in the detection of metastasis of postoperative breast cancer relative to helical CT.