Humans ; Lipid Peroxidation ; Male ; Pneumoconiosis ; metabolism ; Superoxide Dismutase ; blood

Animals ; Hypothermia ; blood ; Hypoxia ; blood ; Malondialdehyde ; blood ; Myocardial Ischemia ; blood ; Rabbits ; Superoxide Dismutase ; blood

Animals ; Insecticides ; toxicity ; Nitric Oxide ; blood ; Organophosphorus Compounds ; Rabbits ; Superoxide Dismutase ; blood ; Vitamin E ; blood

Animals ; Creatine Kinase ; blood ; Lactate Dehydrogenases ; blood ; Lipid Peroxidation ; Male ; Rabbits ; Superoxide Dismutase ; blood ; Weight-Bearing

UNLABELLEDOBJECTIVE To compare anti-oxidative effects of acupuncture at different Shichen (traditional twelve two-hour periods) accordin-17:00), You (17:00-19:00), Xu (19:00-21:00), Hui (21:00-23:00) periods according to the eight methods of the intelligent turtle, once each day, for 7 consecutive days. Changes of malondialdehyde (MDA) content and superoxide dismutase (SOD) activity after treatment were observed.

RESULTSSOD activities and MDA contents at the 12 periods were different in the healthy guinea pigs, SOD activity at Wu period was the highest and the lowest at Zi period; MDA content was the highest at Zi period and the lowest at Wu period. The needling method according to eight methods of the intelligent turtle could increase SOD activity in the guinea pigs. The increasing amplitude of SOD activity was the largest at Mao period and the smallest at the Wu period; it also could decreased MDA content, the decreasing amplitude of MDA was the largest at Wu period and the smallest at Hai period.

CONCLUSIONAcupuncture at different periods according to eight methods of intelligent turtle has different effects on serum SOD and MDA, which can increase SOD activity and decrease MDA content in the healthy guinea pig.

Acupuncture Therapy ; Animals ; Female ; Guinea Pigs ; Male ; Malondialdehyde ; blood ; Superoxide Dismutase ; blood ; Time Factors

Adult ; Humans ; Male ; Malondialdehyde ; blood ; Submarine Medicine ; Superoxide Dismutase ; blood ; Young Adult

OBJECTIVETo explore the level and effect factors of superoxide dismutase (SOD) and malondialchelyche (MDA) of the people aged 90 years old and above in longevity regions in China.

METHODSFrom Xiayi county (Henan province), Zhongxiang county (Hubei province), Mayang county (Hunan province), Foshan county Sanshui district (Guangdong province) and Yongfu county (Guangxi province), the people of hundred-year were selected and numbered. The old people aged 90 - 99 according to the age and sex were appointed by the centenarians' code and 496 people were selected in total. Questionnaire investigation was conducted on nationality, education, nutrition, smoking, alcohol drinking, tea drinking and physical activities.436 blood sampling (4 ml Per sampling) were conducted and the level of triglyceride (TG), SOD, MDA, Ca, Mg, and some microelements were determined. Non-conditional logistic regression analysis was conducted to explore the effect factors of SOD and MDA in plasma.

RESULTSAnalysis showed that the activity of plasma SOD was (31.19 +/- 5.51) U/ml, and the content of MDA was (4.68 +/- 1.98) micromol/L. The SOD level of minority people of 90 years old and over was (27.55 +/- 4.97) U/ml, lower than that of Han nationality ((31.73 +/- 5.39) U/ml; t = 5.85, P < 0.01); the MDA level of minority people of 90 years old and over was (5.58 +/- 1.95) micromol/L, higher than that of Han nationality ((4.54 +/- 1.95) micromol/L; t = -3.94, P < 0.01). logistic regression showed, comparing with the Han nationality, the minority had lower level of SOD (OR = 0.493), and higher level of MDA (OR = 5.412, P < 0.05). Beside nationality, the factors significantly affecting the activity of SOD included the intake frequency of meat, intake frequency of eggs and plasma concentration of Se (OR values were 0.388, 2.260 and 1.906, P < 0.05). The factors significantly affecting the level of MDA included the intake frequency of milk, physical activity and the level of TG (OR values were 0.435, 1.850 and 1.647, P < 0.05).

CONCLUSIONThe level of SOD of the oldest-old people aged 90 years old and over is relatively higher than the younger ones, and the level of MDA is relatively lower in longevity region, China. The effect factors of SOD are nationality, meat intake frequency, eggs intake frequency and the level of Se in plasma. The effect factors of MDA are nationality, milk intake frequency, physical activity and the level of TG in plasma.

Aged, 80 and over ; China ; Female ; Humans ; Longevity ; Male ; Malondialdehyde ; blood ; Plasma ; chemistry ; Superoxide Dismutase ; blood

UNLABELLEDOBJECTIVE; To observe clinical therapeutic effect and the mechanism of drug-separated moxibustion at the navel for delaying aging.

METHODSSeventy-one cases were randomly divided into 3 groups. The group of drug-separated moxibustion at the navel (n=30) were treated with drug-separated moxibustion at the navel, twice each week; the acupuncture group (n=21) with acupuncture at Shenshu (BL 23) and Taixi (KI 3) as main points once each day; the western medicine group (n=20) with oral administration of vitamin E capsules, 0. 1 g each time, twice daily. Their clinical therapeutic effects, and changes of cumulative scores, serum SOD activities and MDA contents after treatment were compared among the 3 groups.

RESULTSAfter treatment, clinical symptoms in the 3 groups very significantly improved (P < 0.01), with the group of drug-separated moxibustion at the navel more significantly improved (P < 0.01); in the 3 groups, SOD activities significantly increased (P < 0.01) and serum MDA contents significantly decreased (P < 0.01), and SOD activity and MDA content in the group of drug-separated moxibustion at the navel were not significantly different from those in the acupuncture group (P > 0.05), but significantly different from those in the western medicine group (P < 0.05).

CONCLUSIONThe drug-separated moxibustion at the navel can significantly delay aging.

Acupuncture Therapy ; methods ; Aged ; Aging ; Female ; Humans ; Male ; Malondialdehyde ; blood ; Middle Aged ; Moxibustion ; Superoxide Dismutase ; blood

AIMTo investigate the mechanism of protective effect of SOD (superoxide dismutase) on damage of RBCs stored at 4 degrees C, the studies of erythrocyte glucose and energy metabolism were performed.

METHODSwhole blood collected from healthy donors and stored at 4 degrees C in ACD, GMA and SOD solutions. Before and post storage, some parameters were assayed. Standard methods were used for the in vitro tests. The 24-hour in vivo recoveries were measured by FTTC (Fluorescein 5-isothiocyanate) from SIGMA Company.

RESULTSAll parameters of red blood cell glucolysis rate without oxygen condition, ATP, PK (pyruvic kinase) and 24 h recoveries level were 86.2%, 56.4%, 64.3% and 86.2% of normal respectively stored in SOD solution at 4 degrees C for 75 days, distinctly more than in ACD and GMA groups at 75 days stored. The 24 h recovery at 75d in group SOD was near the recovery at 42d in group GMA.

CONCLUSIONWhole blood in SOD solution can be stored satisfactorily for 75 days at 4 degrees C, and furnished theoretical evidence for RBCs survival.

Animals ; Blood Preservation ; methods ; Erythrocytes ; cytology ; metabolism ; Humans ; Rabbits ; Refrigeration ; Superoxide Dismutase ; pharmacology

To study the dynamic changes of ultrastructure of erythrocytes in prolonged preservation of blood with preservative fluid containing superoxide dismutase (SOD), the whole blood samples were preserved at 4 degrees C in SOD-containing solution, the morphologic changes of erythrocyte were dynamically ob served by transmission microscopy after preservation for 42, 75 and 85 days, an d the blood samples preserved in GMA solution served as control. Three variance was applied to analyze the data with SAS software. The results showed that the metamorphotic rates of erythrocyte preserved in SOD-containing solution for 42, 75 and 85 days were lower than those of erythrocytes preserved in GMA solution. Most of metamorphotic rates of erythrocyte preserved in SOD-containing solution for 42, 75 and 85 days were correspond to those of erythrocytes preserved in GMA solution for 42 days, or even lower. It is concluded that SOD-containing preservative fluid might help to maintain the normal morphology of erythrocytes in prolonged preservation at 4 degrees C.
Blood Preservation ; Erythrocyte Deformability ; Erythrocytes ; ultrastructure ; Humans ; Microscopy, Electron ; Superoxide Dismutase ; pharmacology ; Time Factors