1.The Therapeutic Effect of Ginkgo Biloba Extract on Hypoxic Ischemic Hepatic Damage of Neonatal Rats
Suoying WANG ; Shaoming SONG ; Jiannong WU
Chinese Journal of Perinatal Medicine 1998;0(03):-
Objective To evaluate the therapeutic effect of extract of ginkgo biloba (EGB) on hepatic damage of neonatal rats with hypoxic ischemic brain damage(HIBD). Methods Sixty six Sprague Dawley(SD) neonatal rats were divided into four groups: groupⅠincluded neonatal rats with hypoxic ischemic hepatic damage after HIBD group I, ( n=30 ), groupⅡ( n=24 ) was those with EGB-treated, groupⅢ ( n=6 ) was those with saline-treated and groupⅣ ( n=6 ) which had sham operation. ICAM-1 of liver was detected at different times in the first two groups after HIBD and the rest groups by immunohistochemical ABC method. Simultaneously HE was employed to display neutrophil infiltrations in hepatic tissue and pathologic characteristics of liver cells. Results The expression of ICAM-1 increased at 24 h [(52.5?17.0)%] with resupply oxygen, peaked at 48 h [ (76.5 ?11.9)%] ( H=23.9,P
2.Adeno-associated virus mediated T-bet gene transfer into SGC-7901 cell to regulate IFN-gamma production.
Gufeng QIU ; Suoying WANG ; Shengjun WANG ; Qixiang SHAO ; Jie MA ; Ming YANG ; Xiaopeng XU ; Chaoming MAO ; Zhaoliang SU ; Xinxiang HUANG ; Huaxi XU
Journal of Biomedical Engineering 2009;26(3):606-619
In order to investigate the effect of T-bet on malignant cells, we selected SGC-7901, a kind of human gastric carcinoma cell line, and used gene clone technique and adeno-associated virus (AAV) packing technology, thus obtaining a recombinant rAAV-eGFP-T-bet and T-bet gene-transfected SGC-7901 cells. Then the function of T-bet gene-infected SGC-7901 cells was researched by detecting the levels of IFN-gamma and T-bet production. The results showed: (1) It was verified that rAAV-T-bet's packing was completed; (2) After SGC-7901 cells was transfected by rAAV-eGFP-T-bet, a green fluorescence was found in about 30%-40% SGC-7901s, and the gene of 1670 bp (T-bet) and 388 bp (IFN-gamma) were generated from SGC-7901s cells; (3) The proteins of IFN-gamma and T-bet secreted by SGC-7901 cells were also detected. These reveal that SGC-7901 cell is efficiently infected by rAAV encoding T-bet, which can induce transfected cells to secret IFN-gamma. It may be useful in the researches on cancer immune therapy of transfecting T-bet gene.
Cell Line, Tumor
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Dependovirus
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genetics
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metabolism
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Green Fluorescent Proteins
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biosynthesis
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Humans
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Interferon-gamma
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biosynthesis
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Recombinant Proteins
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biosynthesis
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genetics
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Stomach Neoplasms
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genetics
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metabolism
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T-Box Domain Proteins
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biosynthesis
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genetics
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Transfection