Hematopoietic Stem Cell Transplantation ; Humans ; Vaccination

Coix ; Epimedium ; Fatigue ; Humans ; Hypoxia ; Plant Leaves

Objective To examine the effect of aqueous and ethanol extracts of Coix lacryma-jobi stem on the growth of mouse S180 sarcoma cells in mice.Methods Tumor growth inhibition assays were performed using both aqueous and ethanol extracts of Coix lacryma-jobi stem.Specific-pathogen-free (SPF) Kunming mice,6-8 weeks old,were inoculated with 1 × 107 cells · mL-1 S180 sarcoma cells in a volume of 0.4 mL The next day,the mice were randomly divided into blank group,control group,experimental-H,experimental-M,experimental-L doses groups,and administered with 0.9％ NaC1,cyclophosphamide (20 μg · g-1 · d-1) or Coix lacryma-jobi stem extract (1 000,500,250 mg · kg-1 · d-1) respectively by gavage or tail vein injection for 10 d.The tumor and spleen were dissected and weighed.The tumor inhibition rate was calculated.Results Tumor growth inhibition ratios in the experimental-H,experimental-M,experimental-L groups by the aqueous extract of Coix lacryma-jobi stem were 32.11％,75.63％ and 11.41％,respectively.Tumor growth inhibition ratios for experimental-H,experimental-M,experimental-L groups by the ethanol extract of Coix lacryma-jobi stem groups were 77.09％,80.03％ and 73.52％,respectively.Conclusion Each dose of water or ethanol extract of Coix iacryma-jobi stem was able to inhibit the growth of S180 sarcoma to a degree,and exhibited few adverse reactions.The optimal dose of the aqueous extract was 500 mg · kg-1 · d-1.

Objective To explore the anticancer effect of petroleum ether fraction of the extract from stems and leaves of Coix lacryma in vitro.Methods The in vitro growth inhibitory effects of different solutions of the extract of Coix stems and leaves on 3 kinds of tumor cell lines was detected by CCK-8 assay.Results Four kinds of Coix extract can inhibit the growth of the tested tumor cell lines in vitro.For the extracts of Coix stems,the half maximal inhibitory concentrations (ICs0) of petroleum ether fractions of the water extract on HeLa and ethanol extract on SGC-7901 were 51.78 and 74.44 μg · mL-1.And,for the extracts of Coix leaves,they were 45.22 and 45.91 μg · mL-1.Conclusion The petroleum ether fractions of the extract of Coix stems and leaves had better anti-tumor activity.

Objective To study the tumor inhibitory effects and an in-fluence on immune organs of the water extract from stem of Coix Lachry-ma-jobi L.(WESCLL) in mice with liver cancer H22.Methods The experimental subjects were using mice with liver cancer H 22.The mice are randomly divided into 7 groups and were fed 0.9%sodium chloride , cyclophosphamide (0.02 g? kg -1 ), WESCLL(2,4,6,8,10 g? kg -1 ) by oral gavages.After 9 days′administration, the mice were killed. Their subcutaneous sarcoma ,liver,thymus and spleen were separated and weighted.Then the tumor inhibiting rates and liver , thymus and spleen index were calculated.Results The WESCLL has obvious effects on re-straining the liver cancer H22.There was significant difference compared with model group (P<0.05).The effect of WESCLL is more noticeable on liver index and weigh′s increase of the mice ( P<0.05 ) .There was not significant difference on thymus and spleen indexes compared with model group ( P<0.05 ) .Conclusion WESCLL have distinct effect on anti-tumor.

Objective To study the antitumor effect in vivo of Coix leaf′s water extract and alcohol extract of different concentrations on H 22 tumor-bearing mice.Methods Take 120 CL Kunming mice to prepare tumor models of cell transplantation , and divided these into 12 groups, with 10 mice in each group.There are model group and control group ( cy-clophosphamide , 20 mg · kg -1 ) , five difference concentration ( 0.15 , 0.30 ,0.45 ,0.60 ,0.75 g · mL -1 ) groups with Coix leaf′s water extract (CLWE) and alcohol extract(CLAE).The tumor of H22 tumor-bearing mice was weighed.The inhibitory rate, and calculate liver index, spleen index and thymus index were calculated .Results The best antitumor concentration of the extract from Coix leaf′s is 0.45 g· mL-1 .The inhi-bition rate of CLAE is better than the CLWE .Compared with the model group, the 0.45 g· mL-1 group of CLWE and each concentration groups of CLAE can significantly inhibit the growth of tumor ( P <0.05 , P<0.01 ) .The tumor inhibition rate of CLAE of 0.45 g · mL -1 reaches 63.2%.The Coix leaf′s extracts exert antitumor effect , and have a sig-nificant protective effect on thymus at the same time .Conclusion The best antitumor concentration of the extract from Coix leaf′s is 0.45 g· mL-1 .The inhibition rate of CLAE is better than that of the CLWE .

Objective:To establish a sequencing method for the genome of severe fever with thrombocytopenia syndrome virus (SFTSV) based on next-generation sequencing (NGS).Methods:SFTSV RNA was extracted from serum samples of patients with severe fever with thrombocytopenia syndrome. SFTSV-specific primers were designed using Primer 5.0 software. A multiplex PCR method was constructed and used to amplify the nucleotide sequence of SFTSV. Whole-genome sequencing was performed on the NGS platform.Results:The whole genes of SFTSV isolates in 28 serum samples were amplified by the multiplex PCR with a coverage over 94%. Sequencing and phylogenetic analysis of those strains revealed that the predominant strains ( n=20) belonged to genotype A, followed by genotypes B ( n=4) and E ( n=3). Conclusions:A high-throughput sequencing method for SFTSV based on multiplex PCR was established in this study. This method was characterized by high specificity and good quality and could improve the sequencing efficiency.

Objective To study the acceptability of pre-exposure prophylaxis (PrEP) to prevent the transmission of HIV among men who have sex with men (MSM) in Guangxi, China.Methods Snow-balling methods were used to recruit 650 MSM in Guangxi. Questionnaires and interview were administrated to these 650 men, using a self-designed questionnaire and face to face interviews to collect information on HIV-related risk behaviors, knowledge and acceptability of PrEP.effective, safe and free of charge', 597 (91.9%) of the 650 MSM claimed that they would accept it,who refused to use it, most of them said that were afraid of the side-effect and doubted on the effectiveness of PrEP. Data from logistic regression analysis showed that those who had found partners through friends (OR=6.21, P=0.020) and those who would advise his friend to use PrEP (OR=39.32, P=0.000) were more likely to accept PrEP. Those who thought they could protect themselves from HIV infection (OR=0.32, P=0.010) or not having sex with the ones who refused to use a condom (OR=0.34, P=0.010) were less likely to accept PrEP. Conclusion Effectiveness, safety and cost seemed to be the main influential factors related to the acceptability of PrEP. Peer education might improve the acceptability of PrEP.

Objective To study on the anti-fatigue and hypoxia tolerance function of Jasmine leaves' water extract in mice.Methods Forty-eight normal male mice were averagely selected and divided into four groups by weight:blank control group,low concentration experimental group,medium concentration experimental group and high concentration experimental group.Jasmine leaves' water extract of different concentrations (0,200,400,600 mg· mL-1 respectively for the four groups) was given into the mice's stomach for a continuous 16 days,each mice was given 10 mL · kg-1 a day.Burdened swimming test and hypoxia tolerance test were conducted in the mice to determine their swimming time and death time.The liver weight coefficient was calculated.Results The burdened swimming time:Compared with blank control group 9.27 min,that were 9.41,15.42,10.63 min in low,medium,high concentrations experimental groups respectively while medium experimental group had significantly (P ＜ 0.05).The hypoxia tolerance time:Compared with blank control group (47.56 ± 5.61)min,that were (56.24 ± 10.99),(47.20 ± 4.51),(53.44 ± 7.56) min in low,medium,high concentrations experimental groups respectively while low experimental group had significantly (P ＜ 0.01).The liver weight coefficient:Compared with blank control group (4.84 ± 0.29) × 10-2,that were (5.20 ± 0.72) × 10-2,(5.43 ±0.39) × 10-2,(5.42 ± 0.57) × 10-2 in low,medium,high concentrations experimental groups respectively while medium,high experimental groups had significantly(all P ＜ 0.01).Conclusion Jasmine leaves' water extract is proved to have anti-fatigue and hypoxia tolerance function in mice.

Objective To explore the effect of Rhubarb and Polygonum cuspidatum and their combination on blood stasis model rats.Methods Forty female SPF rats were chosen and were randomly divided into blank,model,test I,test Ⅱ,test Ⅲ groups,8 rats in each group.The rats of test I,test Ⅱ,test Ⅲ groups were given 30 mg · kg-1 Rhubarb drug,Polygonum,Rhubarb Polygonum cuspidatum (1:1),qd;blank and model groups were given 0.9％ NaCl at 5 mL · kg-1 dose,qd.The rats were treated for consecutive 10 days.After lavage for 1 h,except the blank group,the rats in other groups were given adrennaline hydrochloride 1mg · kg-1 by intraperitoneal injection.After 24 h,they were injected the same dose of adrenaline again.After the acute blood stasis model was prepared,the rats were anesthetized to collect blood from the abdominal aorta After anticoagulation treatment,the hemorheology index and lipid index of all samples were tested by automatic blood rheology tester and automatic biochemical analyzer.Results The blood biscosity in blank and test Ⅲ I groups were (3.26±1.33) and (4.51 ±1.15) mPa · s at 200/s,(4.21 ±1.41) and (5.80 ±1.44) mPa · s at 50/s,(9.75 ±2.97) and (13.27 ±3.14) mPa · s at 5/s,(25.58 ±7.24) and (34.49 ±7.95) mPa · s at 1/s,with significant difference (all P ＜ 0.05).The erythrocyte aggregation index in the blank,test Ⅰ,test Ⅱ groups were (8.04 ± 0.80),(7.39 ± 0.31) and (7.18 ± 0.62),respectively.The difference between the blank and test Ⅰ groups was statistically significant (P ＜ 0.05).The difference between the blank and test Ⅱ groups were highly statistically significant (P ＜0.01).Triglyceride in model,test Ⅱ groups were (0.92 ±0.39) and (0.61 ±0.15) mmol · L-1,with significant difference (P ＜ 0.05).Conclusion Rhubarb,Polygonum cuspidatum,and Rhubarb-Polygonum cuspidatum combination all had good effect on the rats with blood stasis syndrome,but Rhubarb-Polygonum cuspidatum combination (1:1) did not show the obvious synergistic effect.Polygonum cuspidatum can significantly reduce triglycerides,while Rhubarb alone didn't show the role of significant reduction of blood lipids,and in reducing blood lipids,rhubarb and in this regard,Polygonum cuspidatum compatibility didn't show the synergistic effect.