Bacteria have long been the favorite expression system for recombinant protein production. However, the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias, protein folding, phosphorylation, glycosylation, mRNA stability and promoter strength. Factors are cited and the methods to convert to soluble and active proteins are described, for example a tight control of Escherichia coli milieu, refolding from inclusion body and through fusion technology.
Cloning, Molecular ; methods ; Escherichia coli ; genetics ; metabolism ; Gene Expression ; Genetic Vectors ; Glycosylation ; Phosphorylation ; Protein Folding ; Recombinant Proteins ; biosynthesis ; genetics ; metabolism