1.Comparison of an international scale method and a log reduction method for monitoring of early molecular response in chronic myeloid leukemia patients.
Sunhyun AHN ; Young Ae LIM ; Wee Gyo LEE ; Seong Hyun JEONG ; Joon Seong PARK ; Sung Ran CHO
Blood Research 2016;51(1):58-61
No abstract available.
Humans
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive*
2.A Rare Case of Chronic Myelogenous Leukemia and Plasma Cell Myeloma in the Same Patient.
Sunhyun AHN ; Joon Seong PARK ; Jae Ho HAN ; Sung Ran CHO
Annals of Laboratory Medicine 2015;35(3):370-372
No abstract available.
Aged
;
Antineoplastic Agents/therapeutic use
;
Bone Marrow/pathology
;
Fusion Proteins, bcr-abl/genetics/metabolism
;
Humans
;
Imatinib Mesylate/therapeutic use
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications/*diagnosis/drug therapy
;
Leukocyte Count
;
Male
;
Multiple Myeloma/complications/*diagnosis/drug therapy
;
Platelet Count
;
Polymerase Chain Reaction
;
Thrombocytosis/etiology
3.Methods for Flow Cytometric Analysis of T Cell Subsets in HIV-infected Patients: 2-Color versus 4-Color.
Sunhyun AHN ; Seon Joo KANG ; Young Ae LIM ; Wee Gyo LEE ; Sung Ran CHO
Laboratory Medicine Online 2013;3(4):253-258
BACKGROUND: Blood CD4+ T-lymphocyte (T4) count is a major clinical marker for the diagnosis and management of AIDS, and flow cytometry is considered the gold standard for T4 enumeration. Our aim was to compare the 2-color and 4-color flow cytometric methods for T-cell subset analysis in HIV-infected patients. METHODS: T-cell subsets such as T3, T4, T8, and CD3+CD4-CD8- double negative T cells (DN T) were analyzed from the whole blood of 40 HIV-infected patients by using both 2-color and 4-color methods on a Cytomics FC500 analyzer. Statistical analyses using simple linear regression, paired t-tests, and Bland-Altman plots were performed. RESULTS: The measured T3 (%), T4 (%), T4 (/microL), T8 (%), T8 (/microL), and DN T (%) differed significantly between the 2 methods (P<0.05), whereas the T4/T8 ratio did not. T3 (%), T4 (%), T4 (/microL), T8 (%), T8 (/microL), and T4/T8 measured by the 2 methods showed good correlation, with correlation coefficients above 0.96, whereas DN T (%) did not. The mean differences in T4 (%) and T8 (%) were 0.39% (limit of agreement (LoA), -1.64~2.43) and 1.26% (LoA, -3.37~5.89), respectively. CONCLUSIONS: Although there were statistically significant differences in the T cell subsets measured between the 2 methods, the differences were minor, and the 2 methods showed good correlation. As confirmed in this study, DN T (%) estimated by the 2-color method is lower than the actual value. We suggest that although the 2 methods can be used interchangeably, the 4-color method is recommended for the analysis of some specific subpopulations such as DN T (%).
Biomarkers
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Flow Cytometry
;
HIV
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Humans
;
Linear Models
;
T-Lymphocyte Subsets
;
T-Lymphocytes
4.Gender differences in hepatitis A seropositivity rates according to the Republic of Korea’s vaccination policy
Hyunjin SON ; Sunhyun AHN ; Wonseo PARK ; Gayoung CHUN ; Unyeong GO ; Sang Gon LEE ; Eun Hee LEE
Osong Public Health and Research Perspectives 2024;15(2):168-173
Objectives:
This study aimed to investigate differences in the anti-hepatitis A (HAV) antibody seropositivity rate by age and gender.
Methods:
We collected information on anti-HAV immunoglobulin G and immunoglobulin M status from samples submitted for HAV antibody testing in 2012–2022. A total of 1,333,615 cases were included in the analysis.
Results:
By age, the seropositivity rate was represented by a U-shaped curve, such that the rate was low for the group aged 20 to 39 years and higher in those who were younger or older. Over time, the curve shifted rightward, and the seropositivity rate declined gradually in the group aged 35 to 39 years and older. A gender-based difference in antibody seropositivity rate was especially noticeable in the group aged 20 to 29 years. This difference between genders widened in the participants’ early 20s—when men in the Republic of Korea enlist in the military—and the divergence continued subsequently for older individuals.
Conclusion
These results indicate a higher risk of severe infection among older individuals and a gender-based difference in seroprevalence. Therefore, it is necessary to implement policies to promote vaccination in adults.
5.Minor BCR-ABL1-Positive Acute Myeloid Leukemia Associated With the NPM1 Mutation and FLT3 Internal Tandem Duplication.
Moon Jung KIM ; Sunhyun AHN ; Seong Hyun JEONG ; Ja Hyun JANG ; Jae Ho HAN ; Jong Rak CHOI ; Sung Ran CHO
Annals of Laboratory Medicine 2016;36(3):263-265
No abstract available.
Aged
;
Base Sequence
;
Bone Marrow/metabolism/pathology
;
DNA Mutational Analysis
;
Female
;
Fusion Proteins, bcr-abl/*genetics
;
Gene Duplication
;
Humans
;
In Situ Hybridization, Fluorescence
;
Leukemia, Myeloid, Acute/diagnosis/*genetics
;
Multiplex Polymerase Chain Reaction
;
Mutation
;
Nuclear Proteins/*genetics
;
Philadelphia Chromosome
;
fms-Like Tyrosine Kinase 3/*genetics
6.Current routine practice and clinico-pathological characteristics associated with acute promyelocytic leukemia in Korea.
Sunhyun AHN ; Joon Seong PARK ; Seong Hyun JEONG ; Hyun Woo LEE ; Jun Eun PARK ; Mi Hyang KIM ; Yang Soo KIM ; Ho Sup LEE ; Tae Sung PARK ; Eunkyoung YOU ; Insoo RHEEM ; Joowon PARK ; JI Young HUH ; Myung Seo KANG ; Sung Ran CHO
Blood Research 2013;48(1):31-34
BACKGROUND: Acute promyelocytic leukemia (APL) can be life threatening, necessitating emergency therapy with prompt diagnosis by morphologic findings, immunophenotyping, cytogenetic analysis, or molecular studies. This study aimed to assess the current routine practices in APL and the clinico-pathologic features of APL. METHODS: We reviewed the medical records of 48 Korean patients (25 men, 23 women; median age, 51 (20-80) years) diagnosed with APL in 5 university hospitals between March 2007 and February 2012. RESULTS: The WBC count at diagnosis and platelet count varied from 0.4 to 81.0 (median 2.0)x10(9)/L and 2.7 to 124.0 (median 54.5)x10(9)/L, respectively. The median values for prothrombin time and activated partial thromboplastin time were 14.7 (11.3-44.1) s and 29 (24-62) s, respectively. All but 2 patients (96%) showed a fibrin/fibrinogen degradation product value of >20 microg/mL. The D-dimer median value was 5,000 (686-55,630) ng/mL. The t(15;17)(q22;q12 and PML-RARA fusion was found in all patients by chromosome analysis and/or multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), with turnaround times of 8 (2-19) d and 7 (2-13) d, respectively. All patients received induction chemotherapy: all-trans retinoic acid (ATRA) alone (N=11, 26%), ATRA+idarubicin (N=25, 58%), ATRA+cytarabine (N=3, 7%), ATRA+idarubicin+cytarabine (N=4, 9%). CONCLUSION: Since APL is a medical emergency and an accurate diagnosis is a prerequisite for prompt treatment, laboratory support to implement faster diagnostic tools to confirm the presence of PML-RARA is required.
Cytogenetic Analysis
;
Emergencies
;
Emergency Treatment
;
Fibrin Fibrinogen Degradation Products
;
Hospitals, University
;
Humans
;
Immunophenotyping
;
Korea
;
Leukemia, Promyelocytic, Acute
;
Male
;
Medical Records
;
Partial Thromboplastin Time
;
Platelet Count
;
Prothrombin Time
;
Tretinoin
7.Current routine practice and clinico-pathological characteristics associated with acute promyelocytic leukemia in Korea.
Sunhyun AHN ; Joon Seong PARK ; Seong Hyun JEONG ; Hyun Woo LEE ; Jun Eun PARK ; Mi Hyang KIM ; Yang Soo KIM ; Ho Sup LEE ; Tae Sung PARK ; Eunkyoung YOU ; Insoo RHEEM ; Joowon PARK ; JI Young HUH ; Myung Seo KANG ; Sung Ran CHO
Blood Research 2013;48(1):31-34
BACKGROUND: Acute promyelocytic leukemia (APL) can be life threatening, necessitating emergency therapy with prompt diagnosis by morphologic findings, immunophenotyping, cytogenetic analysis, or molecular studies. This study aimed to assess the current routine practices in APL and the clinico-pathologic features of APL. METHODS: We reviewed the medical records of 48 Korean patients (25 men, 23 women; median age, 51 (20-80) years) diagnosed with APL in 5 university hospitals between March 2007 and February 2012. RESULTS: The WBC count at diagnosis and platelet count varied from 0.4 to 81.0 (median 2.0)x10(9)/L and 2.7 to 124.0 (median 54.5)x10(9)/L, respectively. The median values for prothrombin time and activated partial thromboplastin time were 14.7 (11.3-44.1) s and 29 (24-62) s, respectively. All but 2 patients (96%) showed a fibrin/fibrinogen degradation product value of >20 microg/mL. The D-dimer median value was 5,000 (686-55,630) ng/mL. The t(15;17)(q22;q12 and PML-RARA fusion was found in all patients by chromosome analysis and/or multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), with turnaround times of 8 (2-19) d and 7 (2-13) d, respectively. All patients received induction chemotherapy: all-trans retinoic acid (ATRA) alone (N=11, 26%), ATRA+idarubicin (N=25, 58%), ATRA+cytarabine (N=3, 7%), ATRA+idarubicin+cytarabine (N=4, 9%). CONCLUSION: Since APL is a medical emergency and an accurate diagnosis is a prerequisite for prompt treatment, laboratory support to implement faster diagnostic tools to confirm the presence of PML-RARA is required.
Cytogenetic Analysis
;
Emergencies
;
Emergency Treatment
;
Fibrin Fibrinogen Degradation Products
;
Hospitals, University
;
Humans
;
Immunophenotyping
;
Korea
;
Leukemia, Promyelocytic, Acute
;
Male
;
Medical Records
;
Partial Thromboplastin Time
;
Platelet Count
;
Prothrombin Time
;
Tretinoin
8.Immunosuppressive Drug Measurement by Liquid Chromatography Coupled to Tandem Mass Spectrometry: Interlaboratory Comparison in the Korean Clinical Laboratories
Hyun-Ki KIM ; Hyung-Doo PARK ; Sang-Guk LEE ; Hyojin CHAE ; Sang Hoon SONG ; Yong-Wha LEE ; Yeo-Min YUN ; Sunhyun AHN ; Serim KIM ; Sun Min LEE ; Soo-Youn LEE ; Sail CHUN ;
Annals of Laboratory Medicine 2021;41(3):268-276
Background:
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is increasingly used for immunosuppressive drug tests. However, most LC-MS/MS tests are laboratory-developed and their agreement is unknown in different Korean laboratories.This interlaboratory comparison study evaluated test reproducibility and identified potential error sources.
Methods:
Test samples containing three concentrations of tacrolimus, sirolimus, everolimus, cyclosporine, and mycophenolic acid were prepared by pooling surplus samples from patients undergoing routine therapeutic drug monitoring and tested in duplicate in the participating 10 clinical laboratories. Reconstitution and storage experiments were conducted for the commonly used commercial calibrator set. The robust estimators of reproducibility parameters were calculated. Spearman’s rank correlation coefficient (rho, ρ) was used to evaluate the correlation between drugs. Multiple linear regression was used to determine whether the experimental conditions alter the calibration curves.
Results:
The reproducibility coefficient of variation exceeded 10% only for sirolimus concentrations 1 and 2 (10.8% and 12.5%, respectively) and everolimus concentrations 1 and 2 (12.3% and 11.4%, respectively). The percent difference values showed weak correlations between sirolimus and everolimus (ρ = 0.334, P = 0.175). The everolimus calibration curve slope was significantly altered after reconstitution following prolonged 5°C storage (P = 0.015 for 14 days; P = 0.025 for 28 days); the expected differences at 6 ng/mL were 0.598% for 14 days and 0.384% for 28 days.
Conclusions
LC-MS/MS test reproducibility for immunosuppressive drugs seems to be good in the Korean clinical laboratories. Continuous efforts are required to achieve test standardization and harmonization, especially for sirolimus and everolimus.