No abstract available.
Gene Expression*

Hepatits B virus[HBV] is a world wide-pathogen that causes hepatitis. Infection with HBV may be chronic and is associated with subsequent development of hepatocellular carcinoma. In korea the prevalence rate of B hepatitis is estimated to be 7-10%. Despite the severiority of B hepatitis its pathogenesis, chronicity mechanism, prevention and treatment mode have not been developed. The most vexing technical problem in the biology of HBV has been lack of a practical in vitro system that allows infection and replication of this virus under controlled experimental condition. This study was carried out to develop in vitro model of HBV using liver organ culture system. Infection was carried out co-cultivation of 260 micrometer thick-rat liver slices with HBV obtaind from liver biopsy of patient with HBs antigenemia. Aliquots of culture medium were taken in at 6 hour intervals. Hepatitis surface antigen of culture medium in 12 hour incubation increased ab about two folds in comparing with 6 hour incubation. Dot blot analysis of extrachromosomal HBV DNA in cultured rat liver slices using whole HBV DNA as a probe. Fold increment of viral DNA after 12 hours incubation versus 6 hours was measured as 1.7-fold. The presence of viral antigens and Dane particles were observed with immunohistochemical and electron microscopic techniques. In coclusion I present evidece of development for in vitro model of HBV using organ culture system. This system will be useful for understanding the pathogenesis.
Animals ; Antigens, Surface ; Antigens, Viral ; Biology ; Biopsy ; Carcinoma, Hepatocellular ; DNA ; DNA, Viral ; Hepatitis B virus* ; Hepatitis B* ; Hepatitis* ; Humans* ; Korea ; Liver ; Organ Culture Techniques ; Prevalence ; Rats

No abstract available.
Animals ; Mice*

A total of 322 patients with endobronchial tuberculosis (8.1%) out of 3,982 subjects who had a flexible fiberoptic bronchoscopic examination at the Department of Pulmonary Medicine of Hanyang University Hospital between the beginning of March 1982 and the end of Apr11 1996 were included in this study. The peak incidence occurred in the second decade, and the male to female was 1 : 3.0. The barking cough with variable amounts of sputum was the most common chief complaint in 56.9% of the 313 patients. Other complaints included dyspnea, chest pain, fever, hemoptysis, and generalized weakness. Localized wheeze was heard over the chest in 16.9% of the 313 patients. Infiltration/consolidation was the most common roentgenographic finding of the chest in 64.2%. Bronchoscopically, hypertrophy with luminal narrowing was the most common findings in 32.3% of the 322 patients and left main bronchus was the most frequently involved in 24.0%. Using fiberoptic bronchoscopy allows not only substantial meaningful assessment of endobronchial tuberculosis but also makes a differential diagnosis of lung cancer in older patients. We need further evaluations of standard bronchoscopic classification of endobronchial tuberculosis, diagnostic accuracy of endobronchial tuberculosis by PCR, a large prospective study of effects of corticosteroids in endobronchial tuberculosis patients, and appropriate treatment of atelectasis by endobronchial tuberculosis.
Adrenal Cortex Hormones ; Bronchi ; Bronchoscopy ; Chest Pain ; Classification ; Cough ; Diagnosis, Differential ; Dyspnea ; Female ; Fever ; Hemoptysis ; Humans ; Hypertrophy ; Incidence ; Lung Neoplasms ; Male ; Phenobarbital ; Polymerase Chain Reaction ; Pulmonary Atelectasis ; Pulmonary Medicine ; Sputum ; Thorax ; Tuberculosis*

No abstract available.
Adult* ; Humans ; Respiratory Distress Syndrome, Adult*

To investigate the maternal and child factors associated with early detection of cerebral palsy, 74 mothers of cerebral palsy children who were born since January 1, 1980 and being treated at Taegu Rehabilitation Center for the Handicapped, Rehabilitation Center of Taegu University, St. Paul Children's House and Pusan Welfare Association of Cerebral Palsy Children were interviewed from February to April 1987. There is no association between age of child when parents noticed the child's abnormality and educational level of father but it tend to be detected earlier when education level of mother is college or above compared with high school or under. There is a trend of earlier detection of child's abnormality although statistically not significant in case father is professional or managerial worker, monthly income of father is over 610,000 won, child is first-born, age of the parents is 34 years or under, child is a boy, and child has periodic well-baby check-up. The child's abnormality is detected earlier when mothers had 7 prenatal visits or more compared with those who had 6 visits or less (P<0.05). Parents noticed the child's abnormality first in 85.1% of the case whereas doctors detected it first in 2.7% and this percentage was not different whether the child had periodic well-baby check-up or not. The first physician's diagnosis of the children was cerebral palsy in 36.5% and the rest was normal, need for observation, uncertain, etc. Parents took the child to doctor for diagnosis 2-3 months after they noticed the child's abnormality and after the child was diagnosed as cerebral palsy parents either took no therapeutic measure or brought the child to physiotherapy or acupuncture or gave herb medicine before they started specific rehabilitative therapy. For early detection of the cerebral palsy children, teaching of evaluation method for child development should be reinforced both in medical school and clinical training course and should train the specialist for diagnosis and treatment of crippling conditions. Also, public education needed for the importance of early detection of crippling conditions and currently available methods for diagnosis and treatment.
Acupuncture ; Busan ; Cerebral Palsy* ; Child Development ; Child* ; Daegu ; Diagnosis ; Disabled Persons ; Education ; Fathers ; Humans ; Male ; Mothers ; Parents ; Rehabilitation Centers ; Schools, Medical ; Specialization

No abstract available in English.
Fatigue ; Fractures, Stress

No abstract available.

No abstract available.
Paragonimiasis*

BACKGROUND: Genus of Aspergilli are ubiquitous saprophytic molds in nature, but its change from a saprophytic fungus to a pathogenic organism has occurred since the use of various antibiotics. The fungus affects the chronically ill and debilitated population. Recently frequency of the fungal infection is increasing in Korea with abuse of antibiotics and glucocorticoids. METHOD: We analyzed medical records of 52 patients with pulmonary aspergillosis seen at Hanyang University Hospital from 1980 to 1994. The results were as follows; RESULTS: 1) Ages ranged between second to eighth decades with majority(50%) in the fourth to fifth decades. The male to female ratio was 1.1:1. 2) Hemoptysis and productive cough, the leading symptoms, occurred in 42.3% and 25% respectively. 3) On chest X-ray fingings, the characteristic 'fungus ball' pattern were observed in 53.8% of the 52 cases. 4) Sputum culture for aspergilli were positive in 21.6% of the cases. We performed foe needle aspiration in 22 patients and the diagnostic yield was 100%. 5) Thirty-six patients had history of treatment with antituberculous drugs under diagnosis of pulmonary tuberculosis for an average of 27.3 months. But sputum analysis for acid-fast bacilli were positive in 5.6%(2cases of 36cases), and postoperative pathologic findings showed that 38.9% (12 cases of 28cases) were combined with tuberculosis. 6) Right upper and left upper lobes were predominantly involved(34.6% and 19.2% respectively) and lobectomies were performed in 21 cases. 7) Underlying diseases were present in 47 cases and 48.9% of them were pulmonary tuberculosis. CONCLUSION: These results showed that pulmonary aspergillosis usually develops in patients with open cavitary pulmonary tuberculosis. And we must consider the possibility of pulmonary aspergillosis in a patient with hemoptysis and cavitary lung lesion.
Anti-Bacterial Agents ; Aspergillus ; Chronic Disease ; Cough ; Diagnosis ; Female ; Fungi ; Glucocorticoids ; Hemoptysis ; Humans ; Korea ; Lung ; Male ; Medical Records ; Needles ; Pulmonary Aspergillosis* ; Sputum ; Thorax ; Tuberculosis ; Tuberculosis, Pulmonary