1.Detection of HPV in tissue of cervical lesion: Comparative study between in situ hybridization and PCR in situ hybridization.
Jae Wook KIM ; Young Tae KIM ; Sung Eun MOON
Korean Journal of Obstetrics and Gynecology 1999;42(11):2507-2512
OBJECTIVE: The aim of this study was to identify human papilloma virus(HPV) infection in cervical lesions by using polymerase chain reaction in situ hybridization(PCR-ISH) and to compare the results of PCR-ISH with conventional in situ hybridization(ISH) METHODS: Forty-seven randomly chosen neutral-buffered formalhyde-fixed cervical biopsies in which cervical intraepithelial neoplasia and invasive cervical cancer had been diagnosed were tested for HPV DNA by PCR-ISH and ISH. The method of PCR-ISH includes deparaffinization of specimens, PCR amplification of DNA, in situ hybridization and detection of amplified products. RESULTS: The positivity rate of ISH was 36% for all biopsies and 26%, 40% for CIN and invasive cancer respectively. By using PCR-ISH the positivity was 80%, 84% respectively. All PCR-ISH positive cases were ISH positive and no ISH positive was PCR-ISH negative. CONCLUSION: In our study HPV DNA, undetectable by standard in situ hybridization, was detectable by PCR in situ hybridization. Our study shows that PCR in situ hybridization is highly sensitive method for detection of HPV in cervical neoplastic specimens with morphological evidence.
Adenomyosis
;
Biopsy
;
Cervical Intraepithelial Neoplasia
;
DNA
;
Endometriosis
;
Female
;
Humans
;
In Situ Hybridization*
;
Papilloma
;
Polymerase Chain Reaction*
;
Uterine Cervical Neoplasms
2.A case of pompe disease associated with wpw syndrome.
Woo Sung CHUN ; Moon Sung PARK ; Se Wook OH ; Chang Joon KO ; Tai Seung KIM
Journal of the Korean Child Neurology Society 1993;1(1):179-185
No abstract available.
Glycogen Storage Disease Type II*
;
Wolff-Parkinson-White Syndrome*
3.A Randomized Double - Blind Clinical Trial of 2,000cc vs. 4,000cc Preparation with Balanced Lavage Solution (Golytely) for Colonoscopy.
Chan Wook PARK ; Moon Sung LEE ; Jin Hong KIM ; Sung Won CHO ; Chan Sup SHIM
Korean Journal of Gastrointestinal Endoscopy 1991;11(2):369-378
To determine the most effective and tolerable method of preparation with Golytely for colonoscopy, 141 consecutive cases were randomly allocated to drink either 2,000cc(74 cases, Group I) or 4,000cc(67 cases, Group 2), with infusion rate of 1,000cc/h. The reaults were as follows. 1) The rates of effective colonic clearance in group 1 and group 2 was 94.6% and 94.0%, respectively. There is no significance difference between two groups. 2) There was no significant difference in the ingested volume until the first bowel movement and the time required for rectal efflunt to be clear between two groups. 3) Patient acceptance was higher in group 1 than in group 2. 4) Side effects were less noticed in group 1 than group 2, and incraased in body weight (average 0.8 kg) after ingestion of Golytely was observed only in group 2.
Body Weight
;
Colon
;
Colonoscopy*
;
Eating
;
Humans
;
Therapeutic Irrigation*
4.Endoscopic Prosthesis in Malignant Stricture.
Moon Sung LEE ; Chan Wook PARK ; Jin Hong KIM ; Sung Won CHO ; Chan Sup SHIM
Korean Journal of Gastrointestinal Endoscopy 1991;11(2):363-367
Non-operative palliative treatment for malignant colonic obstruction can sometimes be accomplished by the insertion of anorectal tube, endoscopic balloon dilation or endoscopic laser therapy. But these methods have some disadvantages, such as limitaation of activity, need of repetitive treatment and high-risk of perforation. Endoscopic prosthesis is generally accepted as a safe, effective palliative treatment for malignant esophageal stricture, because this method has no above disadvantages. Neverthless, there is only a few experence with endoecopic prosthesis in malignant colorectal stricture over the world. We report two cases which were safely, effectively performed endoscopic prosthesis in palliative treatment for their malignant rectal strictures.
Colon
;
Constriction, Pathologic*
;
Esophageal Stenosis
;
Laser Therapy
;
Palliative Care
;
Prostheses and Implants*
5.Endoscopic Enucleation of a Gastric Lipoma by Strip Biopsy.
Moon Sung LEE ; Chan Wook PARK ; Jin Hong KIM ; Sung Won CHO ; Chan Sup SHIM
Korean Journal of Gastrointestinal Endoscopy 1991;11(2):273-277
Gastric lipomas are rare benign submucosal tumors which can present the diagnostic and therapeutic problems. The preferred treattment is observation or local excision. Since preoperative diagnosis and differentation from malignant tumors can be difficult by use of the conventional diagnostic modalities such as X-ray or endoscopic examination, patients are sometimes subjected to more extensive surgical procedures than warranted. As to the diagnosis of submucosal tumors of the upper digestive tract, a newly developed diagnostic modality of endoscopic ultrasonography(EUS) allows us to visuialize the structures underlying the gastrointestinal wall in a noninvasive maneuver, and can contribute to make differential diagnosis and decision of management. Strip biopsy is an endascopic tissue resection technique which permits resection of both mucosal and submucosal tissue regardless of the morphological type of the lesion, because the submucosal saline injections during the procedures make the flat or depressed lesions to be elevated. Now, we report a 56-yr-old female which had a Yamada type I polypoid gastric submucosal lipoma in the antrum, which could be performed the different diagnosis and successful endoscopic removal by endoscopic ultrasonography and strip biopsy.
Biopsy*
;
Diagnosis
;
Diagnosis, Differential
;
Endosonography
;
Female
;
Gastrointestinal Tract
;
Humans
;
Lipoma*
6.Estimation of the Depth of Invasion in Depressed Type of Early Gastric Cancer by Endoscopic Ultrasonography.
Jin Hong KIM ; Chan Wook PARK ; Moon Sung LEE ; Sung Won CHO ; Chan Sup SHIM
Korean Journal of Gastrointestinal Endoscopy 1991;11(2):261-271
A new method for combining ultrasonography snd endoscopy, endoscopic ultrasonography (EUS), was developed to improve the diagnostic accuracy of ultrasound by direct image of the target lesion via the gastrointestinal lumen. EUS has the unique ability to provide clear sonographic images of the wall structure of the stomach. This capability can be ultilized in assessing the depth of cancer invasion of the stomach by identifying which layer the ultrasonographic change extends to. However, the depressed types of early gastric cancer(EGC) are often accompanied by ulcerative change in the tumor foci. And the ultrasonographic picture is also influenced by the ulcerative change. (continue...)
Endoscopy
;
Endosonography*
;
Stomach
;
Stomach Neoplasms*
;
Ulcer
;
Ultrasonography
7.Cholangiocarcinoma Arising in Choledocal Cyst.
Chan Wook PARK ; Soo Nam MOON ; Jun Sung LEE ; Moon Sung LEE ; Jin Hong KIM ; Sung Won CHO ; Chan Sup SIM ; Chul MOON ; Dong Wha LEE
Korean Journal of Gastrointestinal Endoscopy 1993;13(1):133-136
Choledochal cyst is a relatively rare disease entity considered to be a congenital cyatic dilatation of the common bile duct. Since occurrence of malignant tumor in choledochal cyet was first reported by Irwin and Morrison in 1944, approximately 100 more cases of malignant tumors arising in congenital choledocal cyst have been reported in the world, and the risk of malignant tumor related to choledochal cyst have been reported 2.4-14%. Recently, we experienced a case of adenocarcinoma arising in choledochal cyst in 39 year-old woman who was diagnosed by ERCP and cholangioscopy with forceps biopsy, and was treated with en bloc resection of the choledochal cyst along with the pancreatic head and duodenum.
Adenocarcinoma
;
Adult
;
Bile Duct Neoplasms
;
Biopsy
;
Cholangiocarcinoma*
;
Cholangiopancreatography, Endoscopic Retrograde
;
Choledochal Cyst
;
Common Bile Duct
;
Dilatation
;
Duodenum
;
Female
;
Head
;
Humans
;
Rare Diseases
;
Surgical Instruments
8.Cholangiocarcinoma Arising in Choledocal Cyst.
Chan Wook PARK ; Soo Nam MOON ; Jun Sung LEE ; Moon Sung LEE ; Jin Hong KIM ; Sung Won CHO ; Chan Sup SIM ; Chul MOON ; Dong Wha LEE
Korean Journal of Gastrointestinal Endoscopy 1993;13(1):133-136
Choledochal cyst is a relatively rare disease entity considered to be a congenital cyatic dilatation of the common bile duct. Since occurrence of malignant tumor in choledochal cyet was first reported by Irwin and Morrison in 1944, approximately 100 more cases of malignant tumors arising in congenital choledocal cyst have been reported in the world, and the risk of malignant tumor related to choledochal cyst have been reported 2.4-14%. Recently, we experienced a case of adenocarcinoma arising in choledochal cyst in 39 year-old woman who was diagnosed by ERCP and cholangioscopy with forceps biopsy, and was treated with en bloc resection of the choledochal cyst along with the pancreatic head and duodenum.
Adenocarcinoma
;
Adult
;
Bile Duct Neoplasms
;
Biopsy
;
Cholangiocarcinoma*
;
Cholangiopancreatography, Endoscopic Retrograde
;
Choledochal Cyst
;
Common Bile Duct
;
Dilatation
;
Duodenum
;
Female
;
Head
;
Humans
;
Rare Diseases
;
Surgical Instruments
9.Radiofrequency Catheter Ablation of Atrial Tachycardia.
Shinki AHN ; Moon Hyoung LEE ; Wook Bum PYUN ; Sung Soon KIM
Korean Circulation Journal 2000;30(2):153-165
Radiofrequency catheter ablation (RFCA) has been established as an effective and safe treatment modality for atrioventricular nodal reentrant tachycardia and WPW syndrome. Surgical ablation or direct current catheter ablation had been performed to cure focal atrial tachycardia (AT), however, these treatments had limitations such as the need of open thoracotomy or the risk of barotrauma. RFCA could be an effective treatment modality for cure of AT. We performed RFCA for AT in 22 patients (male 13, mean age 38.1+/-15.4 years) among 831 patients who underwent electrophysiologic study between Jul. 1996 and May. 1999. Clinical pattern of tachycardia was paroxysmal (17 patients) or incessant (mean duration of symptoms, 41.1+/-42.3 months). Associated cardiac diseases were tachycardia-mediated cardiomyopathy (3 patients), aortic stenosis (1 patient) and ventricular septal defect with pulmonic stenosis (1 patient). AT was induced by programmed electrical stimulation in 17 patients: AT in the other 5 patients was incessant. The RFCA was successful in 17 patients (77.3%). The mean interval between atrial electrogram of mapping catheter and P wave of surface ECG was -53.5+/-24.9msec in 17 successful sites. Fractionated atrial activities were invariably found in the successful sites. Successful sites of RFCA for right AT were around coronary sinus ostium (5), crista terminalis (4), lower portion of sinus node (1), inferior portion of tricuspid annulus (1), and His area (1), respectively. In left AT, lateral portion near atrioventricular groove (2), inferoposterior portion (2) and near left atrial appendage (1) were successful site. During follow-up (mean 23 months), one patient had recurrence (recurrence rate 5.9%). RFCA for AT is an effective and curative treatment in selected cases.
Aortic Valve Stenosis
;
Atrial Appendage
;
Barotrauma
;
Cardiomyopathies
;
Catheter Ablation*
;
Catheters
;
Coronary Sinus
;
Electric Stimulation
;
Electrocardiography
;
Electrophysiologic Techniques, Cardiac
;
Follow-Up Studies
;
Heart Diseases
;
Heart Septal Defects, Ventricular
;
Humans
;
Pulmonary Valve Stenosis
;
Recurrence
;
Sinoatrial Node
;
Tachycardia*
;
Tachycardia, Atrioventricular Nodal Reentry
;
Thoracotomy
;
Wolff-Parkinson-White Syndrome
10.Comparison of Hemostatic Effects between Aprotinin and Tranexamic Acid during Open Heart Surgery in Adults.
Korean Journal of Anesthesiology 1998;35(1):108-114
BACKGROUND: Antifibrinolytics such as aprotinin and tranexamic acid have been administered to reduce blood loss of cardiac surgery, but opinions differ regarding the efficacy of each drug. This study was performed to compare the hemostatic effects between aprotinin and tranexamic acid on adult open heart anesthesia and to evaluate their additive effects. METHODS: We randomly allocated 73 patients undergoing coronary artery bypass grafting or double valve surgery to 4 groups. Group I was non-medicated control(n=15), group II(n=21)patients were recipients of a high dose of aprotinin, group III(n=16) patients were recipients of a conventional dose of tranexamic acid, and group IV(n=21) were recipient of both drugs. Cardiopulmonary bypass time, total operation time, hematocrit, platelet count, transfusion amount and 6 hours of postoperative chest tube drainage were measured. RESULTS: The medicated three groups significantly demonstrated less amounts of blood transfusion and blood loss over the first 6 hours at ICU compared to the nonmedicated control group. Total operation times were shorter in group II, III, and IV compared to group I. Use of both agents together was more effective in reducing the total operation time and blood loss compared to tranexamic alone. CONCLUSIONS: We conclude that the use of aprotinin and tranexamic acid result in significant positive hemostatic effects but superiority of one agent vs. the others is not proved. Use of two agents together yield a more positive effective in reducing the operation time and the blood loss compared to single agent alone but further study would be needed to fully confirm.
Adult*
;
Anesthesia
;
Antifibrinolytic Agents
;
Aprotinin*
;
Blood Transfusion
;
Cardiopulmonary Bypass
;
Chest Tubes
;
Coronary Artery Bypass
;
Drainage
;
Heart*
;
Hematocrit
;
Humans
;
Platelet Count
;
Thoracic Surgery*
;
Tranexamic Acid*