1.Effect of Lactococcus lactis 1370 on the formation of artificial plaque.
Jin CHUNG ; Sung Yee YIM ; Jong Suk OH
Journal of the Korean Society for Microbiology 2000;35(1):77-85
Streptococcus mutans is the most important causative bacteria of dental caries among the oral bacteria. Lactococcus lactis 1370 was isolated from the oral cavity of child. The effect of Lactococcus lactis 1370 on the formation of artificial plaque by Streptococcus mutans was studied. 1. The insoluble substances and bacteria were much more attached on the wall of disposable cuvette in the culture of Streptococcus mutans than in the combined culture of Streptococcus mutans and Lactococcus lactis 1370. 2. The mean weight of produced artificial plaque on the wires in the beaker was 131.7 mg in the culture of Streptococcus mutans only, whereas being reduced to 6.4 mg in the combined culture of Streptococcus mutans and Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Lactococcus lactis 1370 cultured in M17 broth containing 0.5% yeast extract and 5% sucrose, the mean weight of produced artificial plaque was 8.0 mg on the wires, whereas being 125.4 mg in the media without culture supernatant of Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 4. When Streptococcus mutans was cultured in the media containing soluble polymer produced by Lactococcus lactis 1370, the mean weight of produced artificial plaque was significantly reduced compared with being cultured in the media without soluble polymer (p<0.05). The viable cell didn't show the significant difference between them after culturing. 5. The soluble polymer produced by Lactococcus lactis 1370 was glucan. 6. The glucan produced by Lactococcus lactis 1370 was water-soluble glucan containing alpha-1,6-glucose linkage as the main linkage. These results suggest that the artificial plaque formed by Streptococcus mutans is inhibited by water-soluble glucan produced by Lactococcus lactis 1370.
Bacteria
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Child
;
Dental Caries
;
Humans
;
Lactococcus lactis*
;
Lactococcus*
;
Mouth
;
Polymers
;
Streptococcus mutans
;
Sucrose
;
Yeasts
2.A Case of the McCune: Albright Syndrome Associated with Activating Mutations of Stimulatory G Protein.
Phil Ho CHUNG ; Jung Kyu WHANG ; Youn Yee KIM ; Ji Ju WHANG ; Chan Moon PARK ; Chang Hoon YIM ; Ho Yeun CHUNG ; Ki Ok HAN ; Hak Chul JANG ; Hyun Koo YOON ; Hun Ki MIN ; Sung Ran HONG ; Young Soon KANG ; In Gul MOON ; In Kwon HAN
Journal of Korean Society of Endocrinology 1999;14(4):779-785
McCune-Albright syndrome (MAS) is a sporadic disease classically including polyostotic fibrous dysplasia, cafe -au-lait spots, sexual precocity, and other hyperfunctional endocrinopathies. Recent investigations suggest an etiological role for activating embryonic somatic missense mutations in the gene for the a subunit of Gs (Gsa), the G protein that stimulates adenylyl cyclase. DNA from bone, ovary, and blood was analyzed by using polymerase chain reaction and sequenced. A embryological somatic mutation of Gsa gene encoding substitution of a Cys for Arg at amino acid 201 from cells of dysplastic bone and ovary was observed, and the distribution of mutant gene reveals mosaic pattern. We report a case of McCune-Albright syndrome with an activating mutation at codon 201 of Gsa subunit on ovary and bone tissue that was experienced recently.
Adenylyl Cyclases
;
Bone and Bones
;
Codon
;
DNA
;
Female
;
Fibrous Dysplasia, Polyostotic*
;
GTP-Binding Proteins*
;
Mutation, Missense
;
Ovary
;
Polymerase Chain Reaction