OBJECTIVES: A different sequence change, in the mitochondrial tRNA gene, has been proposed as a candidate mutation in the sensorineurnal hearing loss. The purpose of current study is to identify the association between the noise-induced sensorineurnal hearing loss and the A to G mutation at nucleotide 3243 of mitochondrial DNA. METHODS: Subjects were established by history and chart review, and audiological and clinical data were obtained. Blood was sampled from 101 controls, 50 noise-induced hearing loss, and 12 sensorineural deafness. The DNA of these individuals was extracted, and mitochondrial genome was analyzed by polymerase chain reaction. Subsequently, the coding sequence of mitochondrial genome was sequenced, and compared to the normal sequence, and all sequence variations were analyzed by restriction endonuclease ApaI. RESULTS: Mitochondrial DNA mutation (3243A->G) was not detected by polymerase chain reaction (PCR) in any patients with noise-induced hearing loss, sensorineural hearing loss, and normal control without hearing loss in Koreans. The DNA sequencing of PCR products did not revealed an A to G substitution at nucleotide 3243 of mitochondrial DNA. CONCLUSIONS: The noise-induced sensorineural hearing loss was not associated with mitochondrial DNA mutation (3243A->G)
Clinical Coding ; Deafness ; DNA ; DNA Restriction Enzymes ; DNA, Mitochondrial* ; Genome, Mitochondrial ; Hearing Loss ; Hearing Loss, Noise-Induced ; Hearing Loss, Sensorineural* ; Humans ; Polymerase Chain Reaction ; RNA, Transfer ; Sequence Analysis, DNA

Impaired sweating reportedly has been associated with a high incidence of heat pyrexia. An important cause is attributed to sweat gland fatigue following intense prolonged sweating. For further investigation, sweat gland fatigue was induced by heat exposure for 2 hours in a hot room (dry bulb 46c, wet bulb 37c) with 9 healthy subjects. Sweating rate of the right forearm increased progressively for the first 45 minutes with a maximum value of 40.0mg/4. 91 cm2/15 min followed by a slow decrease to 24.9 mg/4.91cm2/15 min at the end of 2 hours. Active sweat pores decreased in number following 2 hours of heat exposure but the differences were not significant statistically. In spite of the higher sweat rate on the back, the number of active sweat pores was smaller as compared to the right forearm. Na+ concentrations of the second hour sweat collections were higher than those of the first hour with difference of 14. 9 mEq/l on the right forearm and 12.3 mEq/1 on the back.
Fatigue ; Fever ; Forearm ; Hot Temperature* ; Incidence ; Sweat Glands ; Sweat* ; Sweating

Phakomatosis pigmentovascularis is characterized by the coexistence of pigmentary nevus and hemangioma. Since the term was first described by Ota et al in 1947, about 80 cases have been reported in the literature. In 1979, Hasegawa et al subclsssified the disorder into eight types : type I, nevus flammeus and nevus pigmentosus et verrucosus, type II, nevus flamme is and dermal melanocytosis, type III nevus flammeus and nev.us spilus, type IV, nevus flammeus, dermal melanocytosis and nevus spilus. Each type is subdivided into localized(a) and systemic(b) types, A 25-year-old male had brown papules on the left knee and thigh and purple patches on the left leg that had been present for approximately 7 years. He had no concon ittant systemic disease. The histopathologic findings on brown papules and purple patches are compatible with epidermal nevus and nevus flammeus respectively. We herein report a case of phakomatosis pigmentovascularis type I a.
Adult ; Down Syndrome ; Hemangioma ; Humans ; Knee ; Leg ; Male ; Neurocutaneous Syndromes* ; Nevus ; Port-Wine Stain ; Thigh

Using atomic absorption spectrometry Pb, Cd, Cu, Zn and Fe were measured in sweat and Pb, Cd, Cu and Zn, in blood. The subjects were 22 healthy males, aged between 23-44. Sweating was induced with an environmental room (1 hour at 46C dry bulb and 36C wet bulb). Sweat samples were obtained from the left arm by the armbag technique. The sweat volume was 23.3+7.3ml(n= 22). The concentrations, in ug/ml, of trace elements in sweat were : lead, 0.18+0.17(n=22); cadmium, 0.006+0.006(n=22); copper, 0.37+0.66(n=10); zinc, 0.63+0.5(n=10); and iron, 0.22+0.12(n=10). The concentrations, in ug/ml, of trace elements in blood were : lead, 0.28+0.15(n=20); cadmium, 0.005+0.007 (n=20); copper,0.69+0.22(n=20); and zinc,5.61+0.86(n=20). These results clearly showed the significance of sweating as a route of excretion of trace element.
Absorption ; Arm ; Cadmium ; Copper ; Humans ; Iron ; Male ; Spectrum Analysis ; Sweat* ; Sweating ; Trace Elements ; Zinc

Serum IgE values were determined in 100 patients vith atogic dermatitis in the Department of Dermatology at Severance Hospital, Yonsei University College of Medicine, from Oct. 1, 1978 to Mar. 31, 1980. Mean IgE value of patients is 293. 4 unit/ml which is significantly higher than reference value, and the following results were obtained. 1. The mean IgE value in adults with atopic dermatitis, 339.8 unit/ml, 30 cases: more tian 16 years old) was significantly higher than in children(251. 4 unit /ml, 70 cases). 2. Male patierts showed higher IgE va,lue (mean 324. 5 unit/ml, 62 cases,' than female patients (242.7 unit/ml, 38 cases). 3. No close correlation was demonstrated between IgE values and eosiniphils in blood. 4. Patients with parasitic infesta,tion (5 cases) showed relatively increased IgE values (mean; 116. 2 unit/ml;. 5. Patients wifh bronchial asthma (2 cases) showed high IgE values exceeding 1000 unit/ml, 6. IgE. values roughly correlated with severity of the dermatitis. Four cases with high IgE values exceeding 1000 unit/ml were included in the clinically severe group.
Adult ; Asthma ; Dermatitis ; Dermatitis, Atopic* ; Dermatology ; Female ; Humans ; Immunoglobulin E* ; Male ; Reference Values

Aplasia cutie congenita is a rare congenital disease showing focal abscence of skin. In the msjority of instances it is limited ta the scalp especially on the vertex although other areas of the body may also be involved. Other congenital malformation have been reported to ccur with ACC odcasionally. Diagnosis depends on primarily clinical findings. The histologic finding virious from an absence of appendages to the involvement of varying layers of skin, occasionally extending to the bane or dura. Healed lesions often demonstrate a flattened epidermis, a proliferation of fibrobasts in a loose connective tissue stroma, newly formed capillaries, and a complete absence of adnexa stuctures. We observed a newborn infant with aplssia cutis congenita. The akir, defect was found on the posterior fontanelle. No associating cangenital anomalies were found in this case.
Capillaries ; Connective Tissue ; Cranial Fontanelles ; Diagnosis ; Ectodermal Dysplasia* ; Epidermis ; Humans ; Infant, Newborn ; Scalp ; Skin

Porphyria cutanea tarda(PCT) is a photocutaneous disorder due to excessive porphyrins in the skin. We experienced 4 male patients who showed typical clinical manifestations. Histologic findings revealed subepidermal bullae with festooned derrnal papi11ae, and typical porphyrin excretion pattern of PCT was detected. One case had on 500mg daily of chloroquine with r.linical and biochemical improvement 4 months later without any adverse effects. Other 2 cases had on 125mg of chloroquine twice a week and the other one had perforrned phlebotomy.
Chloroquine ; Humans ; Male ; Phlebotomy ; Porphyria Cutanea Tarda* ; Porphyrias* ; Porphyrins ; Skin

OBJECTIVE: Some growth factors may promote tumor growth by affecting tumor angiogenesis. Midkine(MK) are polypeptides that belong to a new family of heparin-binding growth/differentiation factor and has also been reported to be angiogenic. In various tumor tissues, MK was highly expressed between tumor and normal tissues; however, the pattern of MK expression in normal cervix and cervical cancer has not been established. The aim of this study was to determine the MK mRNA expression in cervical cancer. And we questioned whether its expression is related to cancer stages and prognostic factors. METHODS: The cervical and cervical cancer tissues were taken from patients; healthy women(n=15), and the patients with cervical cancer(n=29). The MK mRNA expression was examined by quantative competitive PCR after polymerase chain reaction amplification of reverse transcriptase copies of RNA transcripts(RT-PCR). RESULTS: The cervical cancer expressed higher levels of MK mRNA than normal cervix(p<0.05). The MK mRNA expression was not correlated with the cervical cancer stage and histopathologic type(p>0.05). CONCLUSION: These results suggested that increased MK mRNA expression is associated with the development of cervical cancer.
Cervix Uteri ; Female ; Humans ; Intercellular Signaling Peptides and Proteins ; Peptides ; Polymerase Chain Reaction ; RNA ; RNA, Messenger* ; RNA-Directed DNA Polymerase ; Uterine Cervical Neoplasms*

No abstract available.
Brain* ; Schizophrenia ; Tomography, Emission-Computed, Single-Photon*

Injectable Collagen Implan, is composed of purified bovine dermal collagen emulsified in saline and lidocaine(0.3%) base, and has been promoted for correction of soft tissue contour defects. Although collagen is a relatively nontoxic, nonimmunologic and biolegically nonreactive biomaterial, some side reactions have been reported. We report herein a case of cutaneous side reaction to collagen implantation in a 38-year-old female. The patient presented with puritic, irritating, multiple, erythematous nodules on both the temporal and interorbital area, which had been observed since 1 day after collagen(KOKEN ATELOCOLLA-GEN IMPLANT-Japan) implantation. Histopathologic findings showed dense infiltration of acute and chronic inflammatory cells in the dermis, especially the perivascular and periadnexal area.
Adult ; Collagen* ; Dermis ; Female ; Humans