To identify the developmental characteristics of fetal skin, the expressions of cytokeratine (CK) and epidermal growth factor (EGFR) in fetal skin (12-24 weeks of gestation) were studied immunohistochemically, and the ultrastructure of epidermis was also observed. The Expressions of CK and EGFR were identified in labelled sterptoavidine biotin immunohistochemical method. Primary antibodies used monclonal mouse anti-human CK (DAKO-CK, MF116) and EGFR Ab-4 which is rabbit affinity-purified polyclonal antibody raised against the amino acid residues 1005-1016 (Onc Science). At 12 weeks of gestation the epidermis was composed basal layer and periderm and the cells of both layers were positively stained for CK and EGFR. At 16-18 weeks of gestation, epidermis was composed basal, intermediate, and periderm. The cells of basal layer and periderm were strongly positive for CK, but the cells of intermediate layer showed weak or negative reaction for CK. EGFR immunoreactivity was noted in cells of all three layers, though cells of basal layer were stained relatively weak. At 23-24 weeks of gestation, the epidermis thickened and appeared 6 or more cell layers. Epidermal cells except horny layer were stained positively for CK and EGFR. EGFR immunoreactivity in basal layer, however, was relatively weak compared to those in intermediate layers. Periderm always were reaction-positive for CK and EGFR. The hair follicles, mainly pre-germ stage, were negatively stained for CK and EGFR at 12 weeks of gestation. The hair follicles with various developing stages were positively stained at 16-18 weeks of gestation. At 24 weeks of gestation, inner sheath of hair shaft and sebaceous gland were strongly reacted for CK, but not reacted for EGFR. In electron microscopic study, epidermis was composed of two layers, basal layer and periderm at 12 weeks of gestation. The periderm was composed of basal, intermediate and periderm at 12 weeks of gestation. The periderm was composed of basal, intermediate and periderm layers at 16-18 weeks of gestation. Intermediate cells consisted of 2-3 layers of spinous cells. The granular cells appeared rarely in superficial cells of intermediate layers. At 23-24 weeks of gestation, epidermis consisted of basal, prickle, granular, and horny layers. Periderm cells were locally exfoliated from the hony layer. The results demonstrate the expression of CK and EGFR in skin of human fetus between 12 and 24 weeks of gestation, and suggest that full thickness of epidermis is formed by 24 weeks of gestation.
Animals ; Antibodies ; Biotin ; Epidermal Growth Factor ; Epidermis* ; Fetus* ; Hair ; Hair Follicle ; Humans* ; Keratins ; Methods ; Mice ; Pregnancy ; Receptor, Epidermal Growth Factor ; Sebaceous Glands ; Skin

We report a case of nevus sebaceus associated with sebaceous epithelioma and basal cell epithelioma in a 48-year-old male patient. A 2.6 x 2.0cm orange-yellow erosive tumor and 1.5 x 1.0cm, l.0 x 0.8cm dark blue-yellow varigated nodules were intimately associated with a 4 x 3cm sized yellowish verrucous plaque on the parieto-occipital area of the scalp. Biopsy revealed sebaccous epithelioma, basal cell epithelioma and nevus sebaceus, respectively.
Biopsy ; Carcinoma* ; Carcinoma, Basal Cell* ; Humans ; Male ; Middle Aged ; Nevus* ; Scalp

We experienced a case of a 30 year-old male with hyperlipoproteinemia associated with xanthoma tuberosum and xanthoma striatum palmare. The laboratory findings showed increased serum cholesterol and triglyceride on lipid profile, and widening of prep band on elec1rophoresis of lipoprotein. The biopsy specimens of orange yellow colored nodules on the elbow and yellow streaks on the palrnar creases showed characteristic findings of xanthoma. He had been treated with gempibrozil(lopid) for 1 month.
Adult ; Biopsy ; Cholesterol ; Citrus sinensis ; Elbow ; Humans ; Hyperlipoproteinemia Type IV* ; Hyperlipoproteinemias ; Lipoproteins ; Male ; Triglycerides ; Xanthomatosis*

BACKGROUND: Hepatitis B vaccination schedule commonly used in Korea is divided largely into 0, 1, 2 month scheduled vaccination group(0, 1, 2 group) and 0, 1, 6 month scheduled vaccination gorup(0, 1, 6 group). The only difference bet.ween two groups is the interval from 2nd dose to 3rd dose. This st,udy had been carried out, to find whether t.he difference of vaccination interval influence the vaccination complet,ion rate or not. METHODS: Study objects are 135 persons over 20 years old who had heptitis B vaccination more than once in injection room of Dongkuk University Kyong-ju Hospital from Jan. 1st in 1996 to Dec. 31th in 1996. Data about vaccination completion were gathered from record book of injection room and telephone interview. RESULTS: Hepatitis B vaccination completion rate is 73.8% in 0, 1, 2 group and 72.5% in 0, 1, 6 group. The reasons for incomplete vaccination are forgetting vaccinat.ion date(36.4% in 0, 1, 2 group and 50% in 0, 1, 6 group), having no time to spare for vaccination(54.5% in 0, 1, 2 group and 43% in 0, 1, 6 group) and knowing positive HBsAb before completion of scheduled vaccination(9.1% in 0, 1, 2 group and 7% in 0, 1, 6 group). CONCLUSIONS: There is no difference in Hepatitis B vaccination complet,ion rate bet.ween 0, 1, 2 group and 0, 1, 6 group.
Appointments and Schedules ; Gyeongsangbuk-do ; Hepatitis B* ; Hepatitis* ; Humans ; Interviews as Topic ; Korea ; Vaccination* ; Young Adult

Carcinosarcomas of the female genital tract have generally been regarded as a type of sarcoma. Recent studies, however, suggest the tumor may be more closely related to carcinoma and may represent metaplastic carcinoma in histogenesis. We analyzed clinicopathologic and immunohistochemical features of 10 carcinosarcomas to evaluate the relative importance of the carcinomatous and sarcomatous components in metastasis and recurrence. The primary tumor originated in the uterine body in seven cases, the uterine cervix in two and the ovary in one. Patient,s ages ranged from 54 to 71 years (mean, 64). The most common symptom of the uterine mass was vaginal bleeding. The median survival time was 21 months following diagnosis in five cases. Surgico-pathologic FIGO stages of five patients who received an operation were stage III and IV, but clinical FIGO stage of three patients (60%) among them were I. Lymphovascular invasions were identified in seven areas; five vascular invasion lesions showed the carcinomatous component alone, one the sarcomatous component alone, and remained one admixture of both components. Metastatic and recurrent lesions to the paraaortic lymph node, ovary, pelvic wall, or vaginal vault showed characteristically carcinomatous component only. Immunohistochemically, positive reactions for cytokeratin and epithelial membrane antigen were noted in the sarcomatous component of five cases. Vimentin positivity was detected in carcinomatous component of three cases. We conclude that the dominant element in carcinosarcomas of the female genital tract is the carcinomatous component. The survival rate of carcinosarcoma is extremely poor. The surgico-pathologic stage is better indicator of survival than the clinical stage. Immunohistochemical findings suggest that carcinosarcoma may represent a metaplastic carcinoma in histogenesis.
Carcinosarcoma* ; Cervix Uteri ; Diagnosis ; Female* ; Humans ; Keratins ; Lymph Nodes ; Mucin-1 ; Neoplasm Metastasis ; Ovary ; Recurrence ; Sarcoma ; Survival Rate ; Uterine Hemorrhage ; Vimentin

We report a case of atrophoderma of Pasini and Pierini in which the characteristic soft atrophic patch with cliffdrop border developed on the neck and two well-demarcated indurated atrophic patches were on both shoulders. We took biopsies from centers and margins of each lesion. Histipathologic findings from atrophic center showed marked dermal atrophy comparing to that from margin of the lesion on the neck, but the atrophic center and margin of the lesion on the shoulder showed morphea-like features without, distinct differences between two.
Atrophy ; Biopsy ; Neck ; Shoulder

Sustained downgaze mostly occurs in association with lesions affecting the dorsal midbrain. We report sustained downgaze in a patient with hepatic encephalopathy. The sustained downgaze existed for seven more days after she regained her consciousness. The persistent downgaze even after regaining full consciousness indicates localized pretectal dysfunction rather than diffuse encephalopathy as the mechanism of sustained downgaze in our patient. The ocular motor dysfunction in hepatic encephalopathy may be due to localized dysfunction of the brainstem

BACKGROUND: Although exact mechanism of adult respiratory distress syndorme (ARDS) is not known, many evidences suggest that inflammatory mediators such as protease and oxygen radicals may be the final common pathway to the endothelial injury. It is gradually accepted that activated neutrophils play a major role in the process. Pentoxifylline is a methylated xanthine with a wide range of actions that make it theoretically useful in the treatment of ARDS. Studies show that it decreases neutrophil phagocytosis and superoxied production. The aim of this experiment is to determine whether pentoxifylline has a preventive effect on oleic acid-induced lung injury. METHODS: Oleic acid (0.08 ml/kg) was infused in 10 mongrel dogs in order to induce acute lung injury. Pentoxifylline (20 mg/kg) was administered 40 mins before oleic acid injection. The author made a comparative studies on the effects of pentoxifylline on hemodynamics and gas exchange before oleic acid injection and at 15, 30, 45, 60, 90 mins after oleic acid injection. RESULTS: There were not significant changes in mean arterial pressure, cardiac output, systemic vascular resistence, pulmonary wedge pressure intrapulmonary shunt in both groups. There were statistical significance in arterial oxygen tension, pulmonary artery pressure, pulmonary vascular resistance between both groups (p<0.05). CONCLUSIONS: Pentoxifylline is a noteworthy drug that could be a candidate as a therapy to help prevent effect in lung injuries that share a common mechanism with oleic acid-induced lung injury.
Acute Lung Injury ; Adult ; Animals ; Arterial Pressure ; Cardiac Output ; Dogs ; Hemodynamics ; Humans ; Lung Injury* ; Lung* ; Neutrophils ; Oleic Acid ; Oxygen ; Pentoxifylline* ; Phagocytosis ; Pulmonary Artery ; Pulmonary Wedge Pressure ; Reactive Oxygen Species ; Vascular Resistance ; Xanthine

BACKGROUND: It is an important task to give adequate nutritions to infants and to wean properly for rapid growth during infancy. Adequate behavior requires precise knowledge. This study was conducted to assess the level of child-caretakers' knowledge about weaning food which is essential to infants' healthy growth. METHODS: From April to June, 1997, we acquired 156 child-caretakers who visited a family medicine clinic in Kyungju and a pediatric clinic in Taegu(from 2months old to 6 years old) medical care. They were asked respond to the pre-made questionnaire. The data were coded and analyzed using Excel and SPSS/PC(version 7.0). Student's t-test was used to test the score difference between two groups by general characteristics. RESULTS: The proportion of correct answers to the question concerning the definition of weaning, was 66.7% ; on proper feeding method when infants do not accept weaning food. 18.5% ; on startion time of weaning food, 64.1% ; on the interval to add new ingredient, 23.1% ; on the sequence of feeding weaning food compared to milk or breast milk. 56.4% ; on How to prepare commercial weaning food, 54.5% ; and to the question in the completing time of weaning food, the proportion of correct answers was 19.2%. The main information sources on weaning food were books and magazines related to child care and rearing(50.6%). The total average score was 4.6/1.0. CONCLUSION: There are many child-caretakers who misunderstand the knowledge of weaning food. Therefore, primary care physician plays an important role in the education of child-caretakers on weaning food.
Child ; Child Care ; Education ; Feeding Methods ; Gyeongsangbuk-do ; Humans ; Infant ; Milk ; Milk, Human ; Periodicals as Topic ; Physicians, Primary Care ; Weaning* ; Surveys and Questionnaires

No abstract available.
Foot ; Hand