One hundred and four biopsy specimens of various dermatoses were stained with Periodic Acid-Schiff and examined. The lesions were grouped into five groups; A. twenty two cases of vesicob-ullous diseases; B. twenty one cases of maculopapulosquamous diseases; C. eighteen cases of chronic granulomatous infection; D. seventeen cases of precancerosis and benign and malignant neoplasms; and E. twenty six cases of miscellaneous skin diseases. Special attention was given to the morph-ological changes of the dermoepidermal junction and the basement membrane. The results were as follows. 1. Among 10 cases of erythema multiforme, partial fraying appeared in 4 cases, partial thicken-ing in 4 and hyperchromasia in 4. Marked convolution was noted in the thickened basement membrane. PAS-positive material was scattered in the edemateus papillary dermis in some cases. 2. All of 6 cases of pemphigus erythematosus and pemphigus vulgaris showed normal basement membrane. 3, In bullous pemphigoid, partial absence in the areas of bulla formation appeared in 2 cases am-ong 3 cases. PAS positive material was present within the bullae and in the upper dermis. 4. All of 3 cases of dermatitis herpetiformis showed partial absence or fraying, 5. Half of 10 cases of psoriasis showed normal basement membrane. Among the remainder, fraying and partial thickening appeared in 3 and 2 cases respectively. 6. Among 6 cases of lichen planus, almsot total absence appeared in 2 cases and fraying in 3 cases. PAS positive material appeared focally in the upper dermis in some cases. 7. Four of 5 cases of pityriasis rubra pilaris showed diffuse thickening and marked convolution ef the basement membrane. 8. Half of 4 cases of lupus vulgaris showed normal basement membrane. Fraying and partial thickening appeared in 2 and 2 cases respectively. 9. All but 1 among 5 cases of lepromatous leprosy showed poor staining and poor visualization of the basement membrane. 10. One case of tuberculoid leprosy showed partial fraying, partial thickening and hyperchromasia with marked convolution, among 5 cases. il. Among 4 cases of condyloma latum, only one showed partial fraying. 12. One of 3 cases of senile keratosis showed partial fraying of the basemement membrane. 13. One case of Bowens disease showed partial thickening and hyperchromasia among 3 cases. PAS-positive material was present focally in the upper dermis in some cases. 14. All of 6 cases of basal cell carcinoma, showed normal basement membrane. PAS-positive ma-terial were present focally in the uppermoet dermis in some vases. 15. All of 3 cases of squamous cell carcinoma showed total absence of the basement membrane. 16. All of the 2 cases of keratoacanthoma showed partial fraying. 17. Among 10 cases of chronic discoid lupus erythematosus, 9 cases showed partial thickening and hyperchromasia with marked convolution of the basement membrane. Two cases showed partial fraying in areas of marked liquefaction degeneration of the basal cells. PAS positive material appe-ared in the uppermost dermis in some cases. 18. All of 3 cases of fixed drug eruption, 2 cases of seborrheic keratosis and 6 cases of verrucae showed normal basement membrane. 19. One case of poikiloderma atrophicans vasculare showed partial fraying, among 2 cases. 20. All of 2 cases of chronic radiodermatitis showed diffuse thickening and hyperchramasia. The thickened basement membrane showed marked convolution focally.
Basement Membrane* ; Biopsy ; Bowen's Disease ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Dermatitis Herpetiformis ; Dermis ; Drug Eruptions ; Erythema Multiforme ; Keratoacanthoma ; Keratosis ; Keratosis, Seborrheic ; Leprosy, Lepromatous ; Leprosy, Tuberculoid ; Lichen Planus ; Lupus Erythematosus, Discoid ; Lupus Vulgaris ; Membranes ; Pemphigoid, Bullous ; Pemphigus ; Pityriasis Rubra Pilaris ; Psoriasis ; Radiodermatitis ; Skin Diseases ; Warts

No abstract available.
Anterior Cruciate Ligament*

The proteins, especially the 19S globulin (IgM) and 7S globulin (IgA) in the CO2 snow-induced blister fluids of 10 male healty volunteers were analyzed immunoelectrophoretically. The fnllawing results were obtained. 1) Compared with autologous serum, all blister fluids tested lacked some protein fraction lines. 2) The 19S globulin (lgM) line was absent in all of the blister fluids except one which was contaminated with blood. 3) The 7S globulin (IgA) line was present in all of the blister fluids when undiluted. In 1 : 2 dilution, however, it was absent, or of trace amount in a few cases.
Blister* ; Humans ; Male ; Volunteers

No abstract available.
Animals ; Macrophages, Peritoneal* ; Mice*

Red blood cells that agglutinate with most normal adult sera but never with own sera are termed polyagglutinable and can be separated by patterns of lectin reactivity into various types. Among these polyagglutination, activation of the T cryptantigen occurs when carbohydrate structures on glycophorins A and B lose sialic acid and express the disaccharide Gal beta-l-3 GalNac which reacts with the peanut agglutinin, a lectin from Arachis hypogaea. T activation is a temporary condition due to exposure of the membrane antigen to the action of microbial neuraminidase. In T activated red cells, the following hazards, which are theoretically possible, are spontaneous polyagglutination of red cells in vitro, in vivo and severe blood transfusion reactions. We experienced a case of T activation in 6 month old girl with bacterial meningitis caused by Streptococcus pneumoniae. The reactivity to lectins indicated the patient's red cells were T activated. We report a case of T activation in an infant with the review of literature.
Adult ; Arachis ; Blood Transfusion ; Erythrocytes ; Female ; Glycophorin ; Humans ; Infant ; Lectins ; Membranes ; Meningitis, Bacterial ; N-Acetylneuraminic Acid ; Neuraminidase ; Peanut Agglutinin ; Streptococcus pneumoniae

Some of the techniques for bone grafting for delayed union or nonunion of the long bone are used currently. In 1991, at arthroscopy workshop in Phoenix, Arizona, Lanny L. Johnson, M.D. reported a case of endoscopic bone grafting for the delayed union on the humerus. He suggested the possibilities of endoscopic bone grafting such as minimal incision, magnified visualization, accurate debridement, accurate graft placement, vascular preservation, outpatient surgery and reduced cost. The author performed endoscopic bone grafting and extra-articular adhesionlysis on the delayed union of femur with extra-articular ankylosis. The patient was a 28 year old housewife with blood type Rh(-). The duration after initial operation was more than 9 months. The result of the graft was satisfactory. In the future endoscopic bone grafting could be performed for nonunion, delayed union and congenital bone defect.
Ambulatory Surgical Procedures ; Ankylosis ; Arizona ; Arthroscopy ; Bone Transplantation ; Debridement ; Education ; Femur ; Humans ; Humerus ; Transplants

No abstract available.
Nipples*

No abstract available.
Benzalkonium Compounds* ; Dermatitis, Contact*

Progressive cribriform and zosteriform hyperpigmentaion (PCZP) is a pigmentary disorder of the skin which was first described by Rower et al in 1978. The disorder is characterized clinically by uniformalyptan cribriform macular pigmentation in a zosteriform distibution and histopathologically by absence of nevus cells and increased melanin in the basal cells. The authors have experienced a case of PCZP in 21 year old Korean man which we believe is the first documented case in Korea. The patient noted light brown macules on his left inguinal area at the age of 17 which has gradually extended to become cribriform macular pigmentation in a zosteriform distribution. Histopathologic examination of the lesion showed an increase in basal melanin pigmentation. No nevus cells were present.
Humans ; Hyperpigmentation* ; Korea ; Melanins ; Nevus ; Pigmentation ; Skin ; Young Adult

The serodiagnostic test of syphilis employed with the greatest freguency in this country is the VDRL procedure which is one of the flocculation test utilizing cardiolipin-lecithin as an antigen. As well known, the immunologically nonspecific nature of this test relegates it only to screening test, so that all the sera displaying weakly reactive or reactive VDRL in the absence of definite evidence of present or past syphilis should be confirmed by using the specific treponemal antigen tests. However, there are only lirnited numbers of institutions at which the specific treponemal antigen tests are carried out, because the tests usually need quite coaiplicatnl technology and specifically trained personnel. The Treponema. pallidum hema.gglutination assay (TPHA) which was first described by Rathlev in 1965 and established as more improved and standardized procedure in serodia,gnosis of syphilis by Tornizaxva and Kasamatsu (1966) has been found to h as sensitive and specific as the technically more complicated Fluorescent treponemaI antibody absorption (FTA-ABS) or Treponema pallidum immobilization (TPI) po cedure by rnany investigators from different parts of the world during these 10 years. The TPHA has also been found to have definite advantage over the other tests because it is easy to perform and economic. The present authors felt. that the TPHA meight be the most reasonable method to be used in this country and decided to evaluate it in the serodiagnosis of problem cases. Sera from 516 presumed normal persons, 686 pregnant women ancl 1345 patients with various diseases other than syphilis were screened with the standard VDRL procedure as described and recommanded by USPHS. Out of these, 475 of t.he presumed normal persons, 646 of the pregnant women and 1243 of the patient group wit;h various diseases other than syphilis v;ere serologically nonreactive and 10, 12 and Rl sera from each of the above groups, respectively, were found to be true syphilitc with definite evidence of clinical signs or history of syphilis. Thirty-one out of the presumed normal persons, 28 out of the pregnant women and 71 out of the patient group with various diseases other than syphilis showed either weakly reactive or reactive VDRI, in undiluted sera without definite evidence of syphilis and these 130 sera from 130 persons were the subject of the present study. The TPHA was carried out on these 130 sera. according to the ma.nual MEA-TP instructed by National Institute of Health, Japan. The reagents used in this study rvere rnanufactured and supplied hy the Fujizoki Pharmaceutical Co. Ten (32.3%) out of the R1 sera from preaumed normal persons, 11 (39.4%) out of the 28 sera from pregna.nt women a.nd 28 (69.4%) out of the patient group with various disea ses other then syphilis showed reactive TPHA. Eighty-seven of the total 130 sera showed weakly reactive VDRL so called rough result and 19 (21.8%) Of these 87 sera showed reactive TPHA, while 30(69.8%) of the 43 sera which showed reactive VDRL were found to be TPHA reactive. These results arc in general agreement with those of other investigators confirmed by the more complicated FTA-ABS or TPI. The overall incidence of syphilis was 3.87%, 3.35% and 4.38% in presumed normal persons, in pregnant women and in patient group with various diseases other than syphilis respectively. Considering the relative lack of study concerning to the false positive reactions in this country, these results may be helpful to the clinician to rnake a cert.ain diagnostic decision when they meet such a patient whose VDRL is weakly reactive or reactive in the absence of definite evidence of syphilis. The authors concluded that: the MHA-TP technique is easy in performance, economic and highly specific in serodiagnosis and that this technique is highly recommanded in this country. * The authors thank Fujizoki Pharrnaceutical Company, Tokyo, Japan which provided the TPHA reagents used in this study.
Absorption ; Bezafibrate ; False Positive Reactions ; Female ; Flocculation Tests ; Humans ; Immobilization ; Incidence ; Indicators and Reagents ; Japan ; Mass Screening ; Pregnant Women ; Research Personnel ; Serologic Tests* ; Syphilis* ; Treponema ; Treponema pallidum ; United States Public Health Service