3.Detection of pemphigus antigens by immunoblot analysis and indirect immunofluorescence using cultured keratinocytes.
Soo Chan KIM ; Jin Hyoung WON ; Sung Ku AHN
Korean Journal of Dermatology 1993;31(3):379-386
BACKGROUND: Pemphigus are chronic autoimmune blistering disorcers characterized by acantholysis. In addition to pemphigus vulgaris(PV), the major clinical variarts are pemphigus foliaceus(PF), paraneoplastic pemphigus(PNP) and drug-induced pemphigus(DP). Detection of pemphigus antigen is important for differential diagnosis as well as research work. Most investigators have identified pemphigus antigens by means of immunoprecipitation using metabolically radiolabeled cultured keratinocytes. However, immunorepitation is generally more expensive, hazardous and time-consuming than immunoblotting. Therefore, establishment of the immunoblotting as a standard technique for the detection of the pemphig us antigens is desirable. OBJECTIVE: To characterized pemphigus antigens by an immunobloting analysis of human epidermal extract and by indirect itnmunofluroscence study using human of cultured keratinocytes as a substraie. METHOD: We performed imrnunoblotting analysis af sera from patieiits with PV, PF, PNP and DP with human epidermal extract as a source of antigen. Indirect immunof uorescence study was also performed using human keratinocytes cultured in high or low calcium media for detection of pemphigus antigens. RESULTS: In an immunoblotting analysis, all(9/9) PV sera showed secific reactivities with a 130-KD protein and all(5/5) FF sera showed reactivities with a 150-KK protein, which is most likely desmoglein 1. Furthermore, one of nine PV serum also reacted with a 150-KD protein, which seems to be the identical antigen detected in PF. All PNP(3/3) sera showed reactivities with two protein bands, 210KD and 190KD. In our indirect imrnunofluorescence study using culltured human keratinocytes as a substrate, when keratinocytes were grown in low calcium media, no pimphigus antigens could be detected. However, when grown in high calciurn media, pemphigus vulga ris and paraneoplastic pernphigus antigens were present t the cell-cell contact areas with a puncta;e pattern, whereas pemphigus foliaceus antigen was not, presint in keratinocytes even when cultured in high calcium media. CONCLUSION: Our results suggests (1) immunoblotting analysis is a reliable technique for defining pemphigus antigen and could be a valuable tool for the differentiation of PV, PF and PNP and(2) PF antigen rnay not be expresseden cultured keratinocytes.
Acantholysis
;
Blister
;
Calcium
;
Desmoglein 1
;
Diagnosis, Differential
;
Fluorescent Antibody Technique, Indirect*
;
Humans
;
Immunoblotting
;
Immunoprecipitation
;
Keratinocytes*
;
Pemphigus*
;
Research Personnel
4.Indirect Particle Agglutination Antibody Testing for Early Diagnosis of Mycoplasma pneumoniae pneumonia in Children.
Jin Soo KIM ; Jeong Hee KO ; Sung Hee OH
Korean Journal of Pediatric Infectious Diseases 2013;20(2):71-80
OBJECTIVES: Outbreaks of pneumonia caused by Mycoplasma pneumoniae (MP) occur every 3-4 years in Korea, most recently in 2011. The aim of our study was to determine the optimal time to perform indirect particle agglutination antibody assays to improve early diagnosis of MP pneumonia in children. METHODS: A database of 206 pediatric patients treated for pneumonia at the Hanyang University Hospital from June to October 2011 was analyzed retrospectively for demographic characteristics and laboratory test results. RESULTS: Among the 206 patients treated for pneumonia during the study period, there were 160 children (mean age, 5.44 years) diagnosed with MP pneumonia, who were studied further. The mean age of these MP pneumonia patients was 5.44 years. Antibody titers increased with increasing time between symptom onset and the collection of serum collection: MP titers were <1:640 for sera collected after 5.44 days and titers > or =1:640 for those collected after 8.58 days; P<0.001). Antibody titers were considered positive when they reached > or =1:640. In 42 MP pneumonia patients in whom there was a four-fold or greater increase in titer between successive serum samples, the optimal cut-off time-point for distinguishing between the initial and second titer groups was 7.5 days after the onset of symptoms (sensitivity, 90.5%; specificity, 92.9%). CONCLUSIONS: Negative MP antibody titers earlier than 8 days after the onset of symptoms in children with pneumonia may require repeating to confirm the diagnosis. This finding could optimize diagnosis and result in better therapeutic outcomes of MP pneumonia in children.
Agglutination
;
Child
;
Disease Outbreaks
;
Early Diagnosis
;
Humans
;
Korea
;
Mycoplasma
;
Mycoplasma pneumoniae
;
Pneumonia
;
Pneumonia, Mycoplasma
;
Retrospective Studies
;
Sensitivity and Specificity
5.Replantation of the amputated finger tip.
Jin Soo KIM ; Jong Ick WHANG ; Sung Ho YOON
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(6):1069-1070
No abstract available.
Fingers*
;
Replantation*
6.Two Cases of Crossed Testicular Ectopia.
Sung Tae CHO ; Byung Soo CHUNG ; Jin Seon CHO
Korean Journal of Urology 2000;41(12):1558-1560
No abstract available.
7.Effect of Clinical Improvement of Schizophrenic Symptoms on 99m Tc-HMPAO Brain SPECT.
Chul Jin SHIN ; Sung Soo KOONG ; In Won CHUNG
Korean Journal of Nuclear Medicine 1997;31(3):310-319
No abstract available.
Brain*
;
Schizophrenia
;
Tomography, Emission-Computed, Single-Photon*
8.MR Findings of Intravertebral Vacuum Cleft: Report of Two Cases.
Sung Moon LEE ; Soo Jhi SUH ; Kyung Jin SUH
Journal of the Korean Radiological Society 1994;31(1):31-34
Intravertebral vacuum cleft in collapsed vertebra was considered as a typical finding of avascular necrosis. However, several authors reported some cases of intravertebral vacuum cleft in primary or secondary neoplasm, or in spondylitis emphasiging the differenhal diagnosis. MRI is known to be a useful diagnostic modality for diferentiation between benign and malignanct conditions causing vertebral collapse. We report MRI findings of two cases with intravertebral vacuum cleft diagnosed as posttraumatic collapse with avascular necrosis on radiological and clinical bases.
Diagnosis
;
Magnetic Resonance Imaging
;
Necrosis
;
Spine
;
Spondylitis
;
Vacuum*
9.Parathyroid cyst.
Jong Sung KIM ; Seok Jin YOUN ; Cheong Soo PARK
Journal of the Korean Surgical Society 1991;40(6):711-715
No abstract available.
10.Fractures of the tibial pilon.
Sung Kwan HWANG ; Jin Soo PARK ; Heui Jeon PARK
The Journal of the Korean Orthopaedic Association 1993;28(5):1747-1757
No abstract available.