To clarify the developmental characteristics of fetal esophageal epithelium especially ciliated cell, expressions of epidermal growth factor receptor (EGFR) and cytokeratin (CK) in fetal esophageal mucosa (16-24 weeks of gestation) were studied immunohistochemically, and ultrastructure of the ciliated cells was also observed. The expressions of EGFR and CK were identified in labelled streptoavidine biotin immunohistochemical method. Primary antibodies used were EGFR (Ab-4) which is affinity-purified from hyperimmune rabbit sera (Oncogene Science) and monoclonal mouse anti-human cytokeratin (DAK0-CK, MNFl16). The esophageal lumen was lined with stratified ciliated columnar epithelium between 16 and 24 weeks of gestation. The pattern of expression Of EGFR was different with gestational age and epithelial layer. The ciliated cell exhibited variable staining intensity for EGFR at 16 weeks. Some were stained intensively, and others were stained faintly. Number of ciliated cells stained intensively were gradually increased, and most of them were strongly stained at 24 weeks. The superficial non-ciliated cells, however, showed relatively constant staining property of moderate to intense between 16 and 24 weeks. EGFR immunoreactivity was minimal in the basal and intermediate cells at 16 weeks, but became more intense at 24 weeks. CK immunoreactivity in the ciliated cells between 16 and 24 weeks was similar to that of EGFR immunoreactivity. On the other hand, superficial non-ciliated cells were intense for CK staining at 16 weeks, but were very weak to negative at 24 weeks. CK immunoreactivity was intense in basal and intermediate cells between 16 and 24 weeks, but it was almost negative in the some cells of intermediate layer, especially beneath negatively stained non-ciliated cells, at 24 weeks. In electron microscopy, ciliated cells had well organized cilia and dense granules close to Golgi apparatus between 16 and 24 weeks. The cells apparently active in ciliogenesis were also observed. These cells had short microvilli, many centrioles, and dense granules close to Golgi apparatus. The non-ciliated cells contained numerous clear vesicles adluminally clustered at 16 weeks, while they had many dense vesicles of about same size of clear vesicles at 24 weeks. These results demonstrate the expressions of EGFR and CK in esophageal epithelium of human fetus between 16 and 24 weeks of gestational ages, and suggest that the ciliated cells are still proliferative at 24 weeks.
Animals ; Antibodies ; Biotin ; Centrioles ; Cilia ; Epithelium* ; Fetus* ; Gestational Age ; Golgi Apparatus ; Hand ; Humans* ; Keratins ; Methods ; Mice ; Microscopy, Electron ; Microvilli ; Mucous Membrane ; Pregnancy ; Receptor, Epidermal Growth Factor

Acquired digital fibrokeratoma is an uncommon, solitary, round, firm, more or less hyperkeratotic projection, most frequently situated on fingers or toes but occasionally on the other locations. It arises out of a collarette of slightly raised skin and may have slight or great resemblance to a rudimentary supernumerary digit or may be mistaken for some other more ordinary condition such as an odd cutareous horn. We experienced a case of acquired digital fibrokeratoma on the right second finger in a 25-year-old male. We treated it with total excision.
Adult ; Animals ; Fingers ; Horns ; Humans ; Male ; Skin ; Toes

Eccrine spiradenoma is clinically characterized by a solitary, movable, tender noduie occurring most frequently on the ventral surfase of the upper body. A striking symptom is paroxysmal pain. Histopathologically, the encapsulated lobules of the tumor are composed of masses of basophilic cells, usually arranged in whorls, cords, and pseudogland.s. Within the whorls and pseudoglands, two types of celIs are notedperipheral intensely staining cells and central pale-staining cells. We describe herein a patient with an indolent eccrine spiradenoma, which had been diagnosed histopathologically.
Basophils ; Humans ; Strikes, Employee

No abstract available.
Breast* ; Hamartoma*

No abstract available.
Adenoma*

OBJECTIVE: To report the efficacy of vaginally administered bromocriptine. MATERIAL AND METHOD: Case report. RESULTS: The prolactin level was significantly decreased after the administration of bromocriptine vaginally. CONCLUSIONS: The vaginal administration of bromocriptine can be an alternative to oral administration in patients with hyperprolactinemia who show severe side effects.
Administration, Intravaginal ; Administration, Oral ; Bromocriptine* ; Humans ; Hyperprolactinemia* ; Prolactin

The soleal line in 115 (left; 61, right; 54) cases of tibiae was studied morphometrically by the method of Mysorekar and Nandedkar. 1. The soleal line showed a uniform character throughout in 26 cases and a mixed characters in 89 cases. The bones showing uniform character were seen generally as a lineal line or wide line. 2. The soleal line having mixed characters divided into three parts. In the upper and middle thirds, the major type of line was wide line. In the lower third, the line was commonly seen as a lineal line. In about 12% of the bones examined the line was seen as a groove, particularly in the middle and lower thirds. 3. The soleal line commenced 1-2cm below the fibular facet In about 61% of cases. 4. The length of the soleal line was 10.5cm, and the ratio to that of the tibia was about 30%. The results of this study provide the characters of the soleal tne of the tibia in Korean. The soleal line, unlike the textbook description, generally shows mixed characters of a line.
Methods ; Tibia*

BACKGROUND: Although a number of indices of diastolic function based on transmitral flow have been proposed, no single factor seems to be adequate for seperating patients with normal from with abnormal diastolic functions. Pulsed Doppler echocardiography of pulmonary venous flow(PVF) is another non-invasive method to evaluate left ventricular diastolic performance. The purpose of this study is to evaluate the normal PVF pattern by TEE. METHOD: We performed pulsed-wave Doppler studies of the PVF and of the mitral flow by transesophageal-(TEE) and transthoracic echocardiography(TTE) in a healthy young adults. RESULTS: In TEE, all sublects showed four phases of the PVE pattern ; two antewgrade systolic phase(early and late : SE and SL), one antewgrade diastolic phase(D) and one retrograde diastolic phase(A). In TTE, there were three phases of the PVF pattern ; two antewgrade phase(systolic, diastolic) and one retrograde diastolic phase but we couldn't find out early systolic phase flow. Peak velocity of each phase of PVF was as follows:SE was 48.9+/-14.1cm/sec, SL was 56.3+/-16.1cm/sec, D was 52.6+/-14.9cm/sec. The timing of SL flow was correlated significantly with that of peak aortic flow(r=0.42, p=<0.01), while the timing of D flow and that of A flow were correlated significntly with timing of mitral E peak and A peak, respectively(r=0.84, p<0.01 ; r=0.80, p<0.01). CONCLUSIONS: In the young normal subject, PVF showed four phase of flow pattern and could be easily obtained by TEE. Furthermore it may be used for evaluation of left ventricular function.
Echocardiography, Doppler, Pulsed ; Echocardiography, Transesophageal* ; Humans ; Ventricular Function, Left ; Young Adult*

PURPOSE: Long-term follow-up results of concurrent bilateral or unilateral cementless hip arthroplasty were compared. MATERIALS AND METHODS: Forty eight and 49 patients with concurrent bilateral and unilateral hip artrhoplasties who had more than a 17-year follow-up time were selected. At final follow-up, mean ages were 69.1 and 66.7 years old in the bilateral and unilateral groups, respectively. Mean follow-up times were 21.5 and 22.4 years in the bilateral and unilateral groups, respectively. Clinical results were attained using Harris hip score and standardized anteroposterior and lateral view X-rays. RESULTS: The bilateral group had mean Harris scores of 44.3(standard deviation 3.78) preoperatively, and 81.2 (10.75) at final follow-up. For the unilateral group, the mean scores were 46.5(3.27) preoperatively, and 80.1 (12.05) at final follow-up. At final follow-up, an acetabular cup was securely located in 78.9% and 82.8% of the bilateral and unilateral groups, respectively, and all cases showed firm fixation of the femoral stem in both groups. Osteolysis around the cup and stem were found in 26.3% and 31.4% of the bilateral group and 16.6% and 17.1% of the unilateral group, respectively. Polyethylene wear rate was 0.17 mm/yr and 0.16 mm/yr in the unilateral and bilateral groups, respectively. Using the Kaplan-Meier method, 17-year survival rates for cup and stem were 78% and 89% in the bilateral group, respectively, and 78% and 86% in the unilateral group, respectively. CONCLUSION: In 17-year long-term follow-up, concurrent bilateral hip arthroplasty was insignificantly different clinically and radiologically from the result of unilateral hip arthroplasty.
Arthroplasty ; Follow-Up Studies ; Hip ; Humans ; Osteolysis ; Polyethylene ; Survival Rate

No abstract available.
Pylorus*