1.Immunohistochemical Demonstration of the Skin Basement Membrane Antigens by the AMex ( Acetone , Methyl Benzoate and Xylene ) Method.
Won HUR ; Joon CHUNG ; Sung Ku AHN ; Dong Sik BANG ; Soo Chan KIM
Korean Journal of Dermatology 1994;32(1):13-23
BACKGROUND: Preservation of antigen determinants while retaining morphological detail is prerequisite for high quality immunohistochemistry. Conventional formalin fixation and paraffin embedding procedures are useful in preserving tissue architecture and cytologic detail. However, they destroy the antigenicity of many proteins is tissue samples. On the other hand, fresh frozen section preserve the antigenicity of most proteins, but vield poor morphological preservation. OBJECTIVE: The purpose of this study is to evaluate the AMeX method as to the ability to preserve both antigenicity and morphologic details of the skin basement membrane zone so that precise localization of antigens can be attained in immunohistochemistry. METHODS: Tissues were fixed in acetone at -20degrees C over night, then cleared in methyl benzoate and xylene, consecutively, and embedded in ordinary paraffin at 58-60degrees C. Sections made from this paraffinembedded tissue were stained with hematoxylin and eosin for a morphologic study and immunolabelled with antibodies against major basement membrane antigens to evaluate antigenic preservation. The staining intensity and preservation of the morphology by the AMeX method were compared with conventional formalin processed tissues and frozen tissues. RESULTS: Morphological preservation of the AMeX method-processed sections was good throughout the epidermis, basement membrane, and dermis, and as good as that of routinely formalin-fixed paraffin-embedded sections. Frozen sections usually revealed revealed various degrees of damage by ice crystal formation throughout the epidermis to the dermis. The AMeX method-processed sections showed better or same antigenic preservation comparing the frozen sections when the sections were immunolabelled with specific monoclonal antibodies. But, when the sections were immunolabelled with patient's sera, the AMex method showed less antigenic preservation than the frozed sections. The anti-type IV collagen monoclonal antibody exhibited immunoreactivity only conventional formalin-fixed paraffin-embedded skin sections, but the intensity of the staining was weaker than the AMeX processed sections and the frozen sections. CONCLUSION: The AMeX method can be utilized for the demonstration of skin basement membrane antigens and is superior to the fresh-frozen method in that the histologic figures are more distinct and antigencity can be preserved for a long time.
Acetone*
;
Antibodies
;
Antibodies, Monoclonal
;
Basement Membrane*
;
Benzoates*
;
Collagen
;
Dermis
;
Eosine Yellowish-(YS)
;
Epidermis
;
Formaldehyde
;
Frozen Sections
;
Hand
;
Hematoxylin
;
Ice
;
Immunohistochemistry
;
Paraffin
;
Paraffin Embedding
;
Skin*
;
Xylenes*
2.A Case Report of Surgical Treatment in Mulitple Tophaceous Gout
Hyeung Seok KIM ; Chang Yong HUR ; Ki Do HONG ; Sung Sik HA
The Journal of the Korean Orthopaedic Association 1987;22(4):974-979
The gout is a hereditary condition of disturbed uric acid metabolism. There are medical and surgical management in tophaceous gout for prevention of recurrence of acute attack and creation of a negative uric acid balance. The surgical management is removal of tophaceous material in the soft tissue and osseous structure. Aditionally, the resection of bone and joint, arthrodesis and bone graft improve the functional capacity of the gouty patients. A 53 years old man was managed by removal of tophaceous materials in both prepatellar area, left olecranon area and both feet and arthrodesis of left first metatarsophalangeal joint with autogenous iliac bone graft and supportive medication with probenecid and indomethacine, and was followed up from March, 1986 to April, 1987. There was no significant difference in duration between the union of nonpathologic fracture and the union of arthrodesis area in this gouty patient. We found the progressive recovery of normal trabeculation in previous destructive bone lesions with drug therapy, but there was no specific change in the soft tissue lesions.
Arthrodesis
;
Drug Therapy
;
Foot
;
Gout
;
Humans
;
Indomethacin
;
Joints
;
Metabolism
;
Metatarsophalangeal Joint
;
Olecranon Process
;
Probenecid
;
Recurrence
;
Transplants
;
Uric Acid
3.An Isolated Fracture-Dislocation of the Cuboid: A Case of Report
Hyung Yeon CHOI ; Kyeong Soo KIM ; Sung Tack HWANG ; Ick Soo KIM ; Eun Sik LEE ; Sung Cho HUR
The Journal of the Korean Orthopaedic Association 1994;29(5):1444-1447
Fracuture-dislocation of the cuboid is an extremely rere injury compared with fracture-dislocation of other tarsal bones. We report a case of the fracture-dislocation of the cuboid treated by open reduction and internal fixation.
Tarsal Bones
4.Safety of Radiofrequency Catheter Ablation in Patients with Supraventricular Tachycardia Confirmed by Means of Echocardiography
Yoon Nyun KIM ; Kee Sik KIM ; Sung Ho HUR
Journal of the Korean Society of Echocardiography 1995;3(1):27-31
BACKGROUND: Radiofrequecy(RF) catheter ablation is the choice of treatment of refractory supraventricular tachycardia. Althogh catheter ablation is invasive, it is well-known that this procedure is safe. However RF catheter ablation may provoke some adverse conditions. The purpose of this study was to assess functional and morphologic changes shortly after radiofrequency catheter ablation in patients with supraventricular tachycardia by means of two-dimensional echocardiography. METHOD: Echocardiogram underwent 1 day before and within 3 days after RF catheter ablation. Cardiac chamber size, wall thickness, and valve motion in the M-mode were Measured. Left ventricular volume, area, dimension, pericardial effusion and segmental wall motion were measured by means of two-dimensional echocardiography and valve incompetence were also measured with color Doppler echocardiography. RESULTS: New echocardiographic abnormalities were observed in 4(5.4%) among 73 patients. One mild aortic regurgitation,one increase in severity of tricuspid imcompetence, one increase in severity of mitral regurgitation and one mild pericardial effusion were found. CONCLUSION: We concluded that echocardiographic changes after radiofrequency ablation are rate and of minor significance.
Catheter Ablation
;
Echocardiography
;
Echocardiography, Doppler, Color
;
Humans
;
Methods
;
Mitral Valve Insufficiency
;
Pericardial Effusion
;
Tachycardia, Supraventricular
5.Kimura's Disease in the Arm
Kye Hyoung LEE ; Hyung Yeon CHOI ; Sung Cho HUR ; Eun Sik LEE ; Kyoung Su KIM ; Doo Hyun CHUNG
The Journal of the Korean Orthopaedic Association 1994;29(6):1564-1570
Kimura's disease is an uncommon, chronic inflammatory disease of unknown etiology. It is an important category of reactive lymphadenopathy in the oriental population. The most common sites are the subcutis of the head and neck, and parotid gland. It's clinical course is benign nature. The treatment modalities for this disease are steroid therapy, radiation therapy and surgical excision. We experienced a case of soft tissue mass in the left arm. It was slightly tender and relatively movable. We excised the mass, which was turned out to be Kimura's disease on microscopic examination.
Arm
;
Head
;
Lymphatic Diseases
;
Neck
;
Parotid Gland
6.The clinical effects of prednisolone withdrawal followed by recombinant alpha-interferon 2b therpy in the patients with CAH type B.
Chong Sun LEE ; Byung Ho KIM ; Ja Won SUNG ; Sung Sik HUR ; Ki Cheon LEE ; Hyun Yong JEONG ; Heon Young LEE ; Young Kun KIM
Korean Journal of Medicine 1993;45(5):588-596
No abstract available.
Humans
;
Interferon-alpha*
;
Prednisolone*
7.Clinical Analysis of Axis Fracture.
Jun Sub LIM ; Sung Chul HUR ; Gi Joong JUNG ; Keong Sik YOUN ; Min Suk OH
Journal of Korean Neurosurgical Society 1997;26(5):656-661
This retrospective analysis describes the clinical characteristics, treatment, and long-term outcome of 30 patients with axis fracture admitted to our institution between January 1991 and December 1995. The incidence of axis fracture was 27.2% in the 110 cervical spine fractures. Among these, odontoid process fracture was the most common type, 19 cases(63%) followed by 8 hangmans fractures(27%), 3 miscellaneous fractures(10%). Hangman's fractures, odontoid type III fractures and miscellaneous fractures were treated with external immobilization devices. Remaining 11 odontoid type II fractures, and six patients with dens dislocation of 6 mm or greater were initially treated by early surgical stabilization. Individuals with dens dislocated less than 6 mm were treated by external immobilization only. Among the nonoperative group of acute axis fractures, there was no fusion failure. In the early operated group, all patients were stable clinically or radiologically. But three patients with posterior wiring and bone graft complained of their neck motion limitation.
Axis, Cervical Vertebra*
;
Dislocations
;
Humans
;
Immobilization
;
Incidence
;
Neck
;
Odontoid Process
;
Retrospective Studies
;
Spine
;
Transplants
8.The Incidence of Feeding Intolerance in the Full Term Neonates with Hypoxic-Ischemic Encephalopathy.
Young Gue SONG ; Sung Dong CHOI ; Jae Kyun HUR ; Chang Kyu OH ; Chung Sik CHUN
Journal of the Korean Society of Neonatology 2002;9(2):192-197
PURPOSE: We investigated incidence of feeding intolerance and time when normal enteral feeding can be established in full term neonates with hypoxic-ischemic encephalopathy (HIE). METHODS: We reviewed medical records of 61 full term infants with HIE who were admitted to St. Paul's Hospital from Jan. 1996 to Dec. 2001. The incidence of feeding intolerance, day of first feeding, and day of full enteral feeding were studied in respective to Sarnat stages. RESULTS: Among the full term with HIE, 32 were classified into stage I, 20 into stage II, and 9 into stage III. The incidence of feeding intolerance was 6%, 30%, and 89% for stages I, II and III, respectively. There was only one case of necrotizing enterocolitis among infants of stage III HIE. Feeding first began on 0.13+/-0.01 postnatal day (PND) in normal infants compared to 0.15+/-0.03 PND in infants of stage I, 3.24+/-1.82 PND in stage II and 5.58+/-2.50 PND in stage III. The incidence of feeding intolerance, day of first feeding, and day of normal enteral feeding achieved in infants with stage I were not different from those of normal infants but significantly higher and delayed in infants with more severe degrees of encephalopathy. CONCLUSION: The first feeding should vary according to severity of encephalopathy so as to lower the incidence of feeding intolerance and the risk of necrotizing enterocolitis. We suggest that infants of stageIencephalopathy be first fed as same as normal infants, but precaution is in order when deciding an appropriate time to start feeding in infants of stage II, III encephalopathy.
Enteral Nutrition
;
Enterocolitis, Necrotizing
;
Humans
;
Hypoxia-Ischemia, Brain*
;
Incidence*
;
Infant
;
Infant, Newborn*
;
Medical Records
9.The Incidence of Feeding Intolerance in the Full Term Neonates with Hypoxic-Ischemic Encephalopathy.
Young Gue SONG ; Sung Dong CHOI ; Jae Kyun HUR ; Chang Kyu OH ; Chung Sik CHUN
Journal of the Korean Society of Neonatology 2002;9(2):192-197
PURPOSE: We investigated incidence of feeding intolerance and time when normal enteral feeding can be established in full term neonates with hypoxic-ischemic encephalopathy (HIE). METHODS: We reviewed medical records of 61 full term infants with HIE who were admitted to St. Paul's Hospital from Jan. 1996 to Dec. 2001. The incidence of feeding intolerance, day of first feeding, and day of full enteral feeding were studied in respective to Sarnat stages. RESULTS: Among the full term with HIE, 32 were classified into stage I, 20 into stage II, and 9 into stage III. The incidence of feeding intolerance was 6%, 30%, and 89% for stages I, II and III, respectively. There was only one case of necrotizing enterocolitis among infants of stage III HIE. Feeding first began on 0.13+/-0.01 postnatal day (PND) in normal infants compared to 0.15+/-0.03 PND in infants of stage I, 3.24+/-1.82 PND in stage II and 5.58+/-2.50 PND in stage III. The incidence of feeding intolerance, day of first feeding, and day of normal enteral feeding achieved in infants with stage I were not different from those of normal infants but significantly higher and delayed in infants with more severe degrees of encephalopathy. CONCLUSION: The first feeding should vary according to severity of encephalopathy so as to lower the incidence of feeding intolerance and the risk of necrotizing enterocolitis. We suggest that infants of stageIencephalopathy be first fed as same as normal infants, but precaution is in order when deciding an appropriate time to start feeding in infants of stage II, III encephalopathy.
Enteral Nutrition
;
Enterocolitis, Necrotizing
;
Humans
;
Hypoxia-Ischemia, Brain*
;
Incidence*
;
Infant
;
Infant, Newborn*
;
Medical Records
10.A case of myasthenia gravis associated with hypothyroidism.
Sang Jun BYEON ; Sang In LEE ; Se Sik CHOI ; Mu Hyun BAE ; Mi Hye JUNG ; Jong Hun KIM ; Sung Pyo SON ; Kap Do HUR
Journal of Korean Society of Endocrinology 1993;8(2):217-220
No abstract available.
Hypothyroidism*
;
Myasthenia Gravis*