1.Immunohistochemical and Electron Microscopic Study on the Epidermis of Human Fetus.
Korean Journal of Physical Anthropology 1994;7(1):109-124
To identify the developmental characteristics of fetal skin, the expressions of cytokeratine (CK) and epidermal growth factor (EGFR) in fetal skin (12-24 weeks of gestation) were studied immunohistochemically, and the ultrastructure of epidermis was also observed. The Expressions of CK and EGFR were identified in labelled sterptoavidine biotin immunohistochemical method. Primary antibodies used monclonal mouse anti-human CK (DAKO-CK, MF116) and EGFR Ab-4 which is rabbit affinity-purified polyclonal antibody raised against the amino acid residues 1005-1016 (Onc Science). At 12 weeks of gestation the epidermis was composed basal layer and periderm and the cells of both layers were positively stained for CK and EGFR. At 16-18 weeks of gestation, epidermis was composed basal, intermediate, and periderm. The cells of basal layer and periderm were strongly positive for CK, but the cells of intermediate layer showed weak or negative reaction for CK. EGFR immunoreactivity was noted in cells of all three layers, though cells of basal layer were stained relatively weak. At 23-24 weeks of gestation, the epidermis thickened and appeared 6 or more cell layers. Epidermal cells except horny layer were stained positively for CK and EGFR. EGFR immunoreactivity in basal layer, however, was relatively weak compared to those in intermediate layers. Periderm always were reaction-positive for CK and EGFR. The hair follicles, mainly pre-germ stage, were negatively stained for CK and EGFR at 12 weeks of gestation. The hair follicles with various developing stages were positively stained at 16-18 weeks of gestation. At 24 weeks of gestation, inner sheath of hair shaft and sebaceous gland were strongly reacted for CK, but not reacted for EGFR. In electron microscopic study, epidermis was composed of two layers, basal layer and periderm at 12 weeks of gestation. The periderm was composed of basal, intermediate and periderm at 12 weeks of gestation. The periderm was composed of basal, intermediate and periderm layers at 16-18 weeks of gestation. Intermediate cells consisted of 2-3 layers of spinous cells. The granular cells appeared rarely in superficial cells of intermediate layers. At 23-24 weeks of gestation, epidermis consisted of basal, prickle, granular, and horny layers. Periderm cells were locally exfoliated from the hony layer. The results demonstrate the expression of CK and EGFR in skin of human fetus between 12 and 24 weeks of gestation, and suggest that full thickness of epidermis is formed by 24 weeks of gestation.
Animals
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Antibodies
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Biotin
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Epidermal Growth Factor
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Epidermis*
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Fetus*
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Hair
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Hair Follicle
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Humans*
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Keratins
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Methods
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Mice
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Pregnancy
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Receptor, Epidermal Growth Factor
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Sebaceous Glands
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Skin
2.Diastematomyelia associated with clubfoot: A Case Report
Young Min KIM ; Moon Sik HAN ; Sung Ho HAN
The Journal of the Korean Orthopaedic Association 1976;11(3):380-382
Diastematomyelia, a form of spinal dysraphism, is characterized by a division of the spinal cord or canda equina resulting the form a bony or cartilaginous spur which transfixes the neural elements and dura. The presence of a structure of neural crest origin in the subarachnoid space connecting the spicules suggested that the anomaly resulted from delayed closure of the neural tube and its investments. The cinical finding are presumed due to its restriction, tethering of the normal apward migration of the spinal cord. Congenital anomalies of one or both lower extremities and spine are frequently found with this condition. The surgical removal is recommended as a prophylactic measure against further progressive neurological damage. The purpose of this paper is to presented becanse it was associated with clubfoot and adds to understanding of teratogenesis of diastematomyelia.
Clubfoot
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Investments
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Lower Extremity
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Neural Crest
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Neural Tube
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Neural Tube Defects
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Spinal Cord
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Spinal Dysraphism
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Spine
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Subarachnoid Space
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Teratogenesis
3.An Application of the Ultrasonograhy to the Musculoskeletal Masses
Sang Hoon LEE ; Heung Sik KANG ; Moon Sung HAN
The Journal of the Korean Orthopaedic Association 1988;23(5):1393-1400
Ultrasonography is suited for screening patients with a mass in the soft tissue because of its safety, low cost, apparent sensitivity and non-invasiveness. But the application in orthopaedic surgery is insufficient. Authors examined 102 patients who had a palpable soft tissue mass of extremities and trunk with ultrasonography. Location, internal echogenicity, margin, and extent of soft tissue involvement, especially neurovascular bundle by tumor were evaluated. The diagnosis of mass was confirmed by histopathology after excision or biopsy of the mass. The ultrasonography correctly identified and differentiated the cystic lesions of 34 cases. The sonograms of all 16 malignant lesions showed ill-defined margin and heterogenous internal echo. The extent of soft tissue involvement, especially neurovascular bundle was confirmed exactly with ultrasonography. Therefore, in malignant tumor, after confirmation of no neurovascular involvemment with ultrasonography, limb-salvage operation could be considered and planned. In one case of abscess, percutaneous biopsy was done under guidance of ultrasonography. Therefore, ultrasonography can be used as a guide for percutaneous biopsy without exposure to radiation.
Abscess
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Biopsy
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Diagnosis
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Extremities
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Humans
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Mass Screening
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Ultrasonography
4.Anti-pre-S2 and anti-HBS responses to a heat inactivated hepatitis B vaccine in patients with mental retardation.
Yong Sung LEE ; Keon Sik MOON ; Han Chul SON ; Soon Ho KIM ; Doo Sung KIM
Korean Journal of Clinical Pathology 1992;12(1):105-113
No abstract available.
Hepatitis B Vaccines*
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Hepatitis B*
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Hepatitis*
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Hot Temperature*
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Humans
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Intellectual Disability*
5.Anti-pre-S2 and anti-HBS responses to a heat inactivated hepatitis B vaccine in patients with mental retardation.
Yong Sung LEE ; Keon Sik MOON ; Han Chul SON ; Soon Ho KIM ; Doo Sung KIM
Korean Journal of Clinical Pathology 1993;13(1):105-113
No abstract available.
Hepatitis B Vaccines*
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Hepatitis B*
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Hepatitis*
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Hot Temperature*
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Humans
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Intellectual Disability*
6.Clinical significance of early diastolic notch of uterine artery Doppler velocimetry in relation to placental location.
Yong Won PARK ; Jae Sung CHO ; Sung Sik HAN ; Jae Wook KIM
Korean Journal of Obstetrics and Gynecology 1999;42(11):2486-2491
OBJECTIVE: Our purpose was to investigate the relation between types of early diastolic notch and placental location and to evaluate the differences in perinatal outcomes. METHODS: We evaluated the Doppler waveform of the uterine artery in 149 pregnant women having an early diastolic notch after 26 weeks gestation, however we were unable to follow the obstetric outcomes in 20 women. Placental location was determined by transverse view of ultrasonography and classified as central and lateral placenta. The unilateral diastolic notches were divided into 3 groups depending on the placental location (ipsilateral: same side of the placenta, contralateral: opposite side of the placenta, and median: central placenta) RESULTS: In the bilateral notch group, the S/D ratio of the contralateral uterine artery was higher than the ipsilateral one(n=60, mean=3.22 Vs 2.80, p=0.0067). Of the 89 unilateral notches observed, the S/D ratio of the uterine artery in patients having early diastolic notch was higher compared to those without early diastolic notch.(t-test, mean=2.74,1.92, p=0.0001) Patients with bilateral notches were associated with poor perinatal outcomes significantly more than the patients with unilateral notches(p=0.003). Among the unilateral groups (ipsilateral: n=10, median: n=21, contralateral: n=58), there was no significant difference in perinatal outcomes. CONCLUSION: The early diastolic notch can be observed on both or either sides of the uterine artery independent on placental location. More bilateral notches were associated with poor pregnancy outcomes than unilateral notches. The pregnancy outcomes tended to be similar in unilateral notch irrespective of placental location, but showed worse outcomes than the control group. Careful analysis of the uterine artery in nonplacental side is necessary because of the frequent appearance of early diastolic notch and higher vascular resistance.
Female
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Humans
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Placenta
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Pregnancy
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Pregnancy Outcome
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Pregnant Women
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Rheology*
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Ultrasonography
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Uterine Artery*
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Vascular Resistance
7.Spinal tuberculosis; gadolinium-enhanced MRI.
Sung Moon KIM ; Heung Sik KANG ; Kee Hyun CHANG ; Moon Hee HAN ; Man Chung HAN
Journal of the Korean Radiological Society 1991;27(4):567-571
No abstract available.
Magnetic Resonance Imaging*
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Tuberculosis, Spinal*
8.A Case of Isolated Left Ventricular Diverticulum in an Adult.
Jae Sung KIM ; Youn Jung KIM ; Keon Sik MOON ; Choon Ho HAN ; Hun Sik PARK ; Hweung Kon HWANG
Korean Circulation Journal 2000;30(4):484-486
Congenital diverticulum of the left ventricle is a very rare disease. Ventricular diverticulum is usually associated with other anomalies including intracardiac and midline thoracoabdominal defect. We describe a case with congenital left ventricular diverticulum presenting as an isolated lesion. A 37-year-old man presented with 4-year history of chest pain. Diverticulum was diagnosed by echocardiography and left ventriculogram.
Adult*
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Chest Pain
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Diverticulum*
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Echocardiography
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Heart Ventricles
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Humans
;
Rare Diseases
9.Spontaneous left external iliac vein rupture.
Ick Hee KIM ; Gyu Rak CHON ; Yoon Sik JO ; Sung Bae PARK ; Sang Don HAN
Journal of the Korean Surgical Society 2011;81(Suppl 1):S82-S84
We report a 72-year-old female patient with spontaneous rupture of the left external iliac vein. She visited our hospital for abdominal and back pain. She had the abnormal finding of hemoperitoneum. We performed an emergency operation with diagnosis of left ovarian cyst rupture though she suffered from spontaneous rupture of the left external iliac vein. This case provides insight to the experience of spontaneous rupture of the left external iliac vein.
Aged
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Back Pain
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Emergencies
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Female
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Hemoperitoneum
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Humans
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Iliac Vein
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Ovarian Cysts
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Rupture
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Rupture, Spontaneous
10.Shake test in preterm and term pregnancy.
Hack Hee LEE ; Buyng Soo HAN ; Hong Sik PARK ; Sung Jin CHO
Korean Journal of Obstetrics and Gynecology 1992;35(11):1592-1596
No abstract available.
Pregnancy*