A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of 22 x 40 mm in size was mounted at a height of 0.8 mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of copepods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to copepods during the handling process.
Protozoa ; Male ; Female ; Culture Techniques/*methods ; Culture Media ; Copepoda/*growth & development ; Animals

No abstract available.
Oropharynx* ; Tongue*

No abstract available.
Hand*

PURPOSE: The purpose is to aid in the diagnosis and obstetric management by analysing the ultrasonographic findings of prenatally diagnosed Edward syndrome. METHOD: The study population consisted of 15 cases prenatally diagnosed Edward syndrome from January 1993 to January 1999. The medical charts and ultrasonographic findings were reviewed retrospectively, and especially the ultrasonographic findings before prenatal genetic diagnosis were anlyzed. RESULTS: All of the 15 cases reviewed showed at least one ultrasonographic finding suggesting Edward syndrome. In the first trimester, 2 cases revealed cystic hygroma and increased nuchal translucency, and after the second trimester, major structural anomalies including congenital heart anomalies and minor ultrasonographic findings such as choroid plexus cyst were noted. All of cases after 21 weeks of gestation showed congenital heart anomaly, and as the pregnancy progressed there was an increased tendency of intrauterine growth restriction and polyhydramnios. CONCLUSION: Prenatal ultrasonography in the screening process of Edward syndrome can be placed as a very informative method, as all of the Edward syndrome cases revealed at least one abnormal ultrasonographic findings. As congenital heart anomalies were found in all of the cases after 21 weeks of gestation, fetal echocardiography should be considered in evaluating suspected cases of Edward syndrome.
Choroid Plexus ; Diagnosis ; Echocardiography ; Female ; Heart ; Humans ; Lymphangioma, Cystic ; Mass Screening ; Nuchal Translucency Measurement ; Polyhydramnios ; Pregnancy ; Pregnancy Trimester, First ; Pregnancy Trimester, Second ; Retrospective Studies ; Ultrasonography* ; Ultrasonography, Prenatal

No abstract available.
Diagnosis* ; Heart Failure* ; Heart Septal Defects, Ventricular ; Heart*

In the treatment of congenital heart in Korea, a big progress has made so far, and successful results have been achieved. However, there were few reports pertaining to the age and cause of death in congenital heart disease patients. Now, the author made observation on the mortality, the age and the mode of death in 3817 patients of congenital heart disease who had been admitted to Severance Hospital during the period of 15 years, from Jan 1972 to Dec 1986. The overall hospital mortality of the congenital heart disease was 6.1% ; 10.1% in the unoperated cases and 4.5% in the patients who underwent surgery. The hospital mortality of indivisual congenital heart disease was, in the order of increasing frequency, 1.0% for ventricular septal defect, and for the complicated heart disease, such as transposition of great vessels(12.5%), and total anomalous pulmonary venous return(15.8%) the death rate was higher in general. The mortality was highest under 1 year of age, especially during the neonatal period, i.e. under 1 month of age. The mode of death in the unoperated cases were congestive heart failure, infection, and hypoxia, in the order of frequency, comprising 84.5%, and in the patients who underwent surgery were congestive heart faliure or low cardiac output syndrome and htpoxia, 62.3%. Among the patients who died in the neonatal period, 76.9% died without accurate diagnosis, and 38.5% were under 5 days of age. Hypoxia(27%), congestive heart faliure(19.4%), and sepsis(11.6%) were the main mode of death. The analysis of the dead patients with congenital heart disease revealed the following results.The mortality was high in the complicated heart disease ; the major mode of death in surgical patients were congestive heart faliure or low cardiac output syndrome ; and the mortality was high in patients under one year of age, especially in the neonates, and most of then lacked accurate diagnosis.Therefore, more accurate properative diagnosis of complicated heart disease and better surgical techniques, development of diagnostic tools in neonatal period, and the need for early surgery and emergency medical management are uregently demanded.
Anoxia ; Cardiac Output, Low ; Cause of Death ; Child* ; Diagnosis ; Emergencies ; Estrogens, Conjugated (USP) ; Heart ; Heart Defects, Congenital* ; Heart Diseases ; Heart Failure ; Heart Septal Defects, Ventricular ; Hospital Mortality ; Humans ; Infant, Newborn ; Korea ; Mortality

No abstract available.
Mastoid*

Purpose: The diagnostic usefulness of fetal echocardiography in congenital heart disease is well known. One of the indications for fetal echocardiography is presence of extracardiac anomalies and such coexistent congenital heart disease may have important implications for obstetric and neonatal management. MATERIALS AND METHODS: 496 women with singleton pegnancies with amgenital anomalies that underwent penatal care and follow-up in Severance Hospital for 5 years from 1991 to 1995 were evaluated for the incidence of congenital heart disease. RESULTS: The incidence of congenital heart disease in fetuses with the associated extracardiac anomalies were as follows; central nervous system 6.9%(11/160), gastro-intestinal system 10.7%(8/75), genito-urinary sysem 4.7%(4/85), others 5.6%(4/72). Thirty-one of eighty-ane fetuses with congenital heart disease were found to have the associated extracardidac anomalies and ten of them chenoscenal abnormalities. CONCLUSION: We recommend that fetuses with congenital extracardiac anomalies sbould be checked for the presence of congenital heart disease and chmmosomal abnormalities, if needed.
Central Nervous System ; Echocardiography ; Female ; Fetus ; Follow-Up Studies ; Heart Defects, Congenital* ; Humans ; Incidence

No abstract available.
Adenocarcinoma* ; Optic Nerve*

BACKGROUND: Vasopressin has recently been recognized to have greater effect on improving blood flow to myocardium and brain during cardiac resuscitation than epinephrine and also improves rates of ROSC(return of spontaneous circulation) and survival in pre-hospital and in-hospital prolonged refractory cardiac angst patients who did not respond to the standard epinephrine treatment. This study was designed to investigate the effects of vasopressin on ROSC rates and survival rates in rat asphyxia arrest model. METHOD AND MATERIAL: Thirty male Sprague-Dawley rats were used. Anesthesia was induced with halothane and nitrous oxide and ventilatory cairo was maintained. EtCO2 was adjusted to 30-40mmHg and halothane was maintained. Right infernal carotid artery and right femoral vein were cannulated and EKG electrodes were attached. After 10 minutes of asphyxia, group 1 was treated with 1ml of saline, group 2 with 1ml(0.001mg/100g) of epinephrine and group 3 with 1ml(0.16u/100g) of vasopressin for resuscitation. Statistical significance was an analysed by SPSS with ANOVA and chi-square tests. RESULTS: No significant differences were seen in baseline measurements. Three ROSC and eight 60 minutes survivals were found in group 1, whereas nine ROSC and eight 60 minutes survivals were obtained in group 2 and all of the subjects in group 3 showed ROSC and 60 minutes survival, but no statistical differences were seen between group 2 and 3. CONCLUSION: Vasopressin seems to have similar effect on improving ROSC and survival rates compared to epinephrine in rat asphyxia models.
Anesthesia ; Animals ; Asphyxia* ; Brain ; Carotid Arteries ; Electrocardiography ; Electrodes ; Epinephrine* ; Femoral Vein ; Halothane ; Humans ; Male ; Myocardium ; Nitrous Oxide ; Rats* ; Rats, Sprague-Dawley ; Resuscitation* ; Survival Rate ; Vasopressins*