1.Isolation and Identification of Melanosomes from Human Hair.
Sung Bin IM ; Sung Nack LEE ; Kyu Woong HAHN
Annals of Dermatology 1991;3(1):12-14
Melanosomes were isolated from the human hair by graded centrifugation and identified by transmission and scanning electron microscopic examination. Melanosomes were separated from the keratinous structures by treating with strong NaOH solution for 15 hours. The keratinous structures were removed by centrifugation ai 2,500xg and 3,500xg for 10 minutes respectively at 0 ℃. The isolated melanosomes were collected by centrifugation at 7,800xg at 0 ℃. Scanning electron microscopic examination made it possible to evaluate the global structure of purified melanosomes.
Centrifugation
;
Hair*
;
Humans*
;
Melanosomes*
;
Microscopy, Electron, Scanning
2.CT diagnosis of the fat containing mediastinal masses
Kyung Soo LEE ; Sung Hoon CHUNG ; Jung Gi IM
Journal of the Korean Radiological Society 1985;21(6):945-953
Fat containing masses, except mediastinal lipomatosis, of the thorax are uncommon. In spite of uncommonoccurences, as CT can detect not only the fat content but also the presence and character of the non-fattycomponent of the mass, accurate preoperative diagnosis can usually be made in most cases of fat containingmediastinal masses. Authors report 6 cases of fat containing mediastinal masses, that were diagnosedpreoperatively by CT scan, comprising 2 cases of limpoma(combined with plexiform neurofibroma in a case), 1liposarcoma, 1 thymolipoma, and 2 cases of omental hernia through Morgagni foramen.
Diagnosis
;
Hernia
;
Lipomatosis
;
Neurofibroma, Plexiform
;
Thorax
;
Tomography, X-Ray Computed
3.Type III Hyperlipoproteinemia.
Beom Joo LEE ; Seung Kyung HANN ; Sung Ku AHN ; Sung Bin IM ; Seung Hun LEE
Korean Journal of Dermatology 1993;31(2):236-240
We report a case of type III hyperlipoproteinemia which is called a broad-beta disease. A 53 year old female patient visited our clinic for the evaluation of multiple yellowish papules on extremities and eyelids. The patient showed various types of xanthoma includiiig eruptive, tuberous, tendinous xanthomas and xanthelasma palpebrarum, xanthoma striatum palmare. The blood chemistry revealed a marked elevstion of cholesterol and triglycerides and agarose gel electrophoresis showed a single peak at prebeta and beta portion without separation. On histopathologic studies, typical foam cells were showen.
Chemistry
;
Cholesterol
;
Electrophoresis, Agar Gel
;
Extremities
;
Eyelids
;
Female
;
Foam Cells
;
Humans
;
Hyperlipoproteinemia Type III*
;
Hyperlipoproteinemias
;
Middle Aged
;
Triglycerides
;
Xanthomatosis
4.Neonatal gastric perforation with diaphragmatic eventration: a case report.
Yeon Im LEE ; Sung Tae OH ; Kyung Kuk KIM ; Kyung Rae KIM ; Yong Kak LEE
Journal of the Korean Surgical Society 1993;45(1):140-145
No abstract available.
Diaphragmatic Eventration*
5.Treatment of Cutaneous Vascular Lesions by the Flashlamp Pulsed Tunable Dye Laser ( SPTL - 1 ).
Seok June LEE ; In Joon LEE ; Sung Bin IM ; Yoon Kee PARK
Korean Journal of Dermatology 1995;33(5):859-866
BACKGROUND: Recent ad vances and innovation in laser technology encourages us to learn and use it experimentally and clinically. The pulsed dye laser(Candela SPTL-1) is a flashlamp excited dye laser, using the princ ples of selective photothermolysis that targets the abnormal microvascu lature of cutaneous lesions while sparing adjacent structures including the epidermis. The laseemits a 585nm wavelength, and has a pulse duration of 450 usec. It is now being widely used in the treatment of vascular diseases in the dermatology field. OBJECTIVE & METHOD : Buring the period of about 3 years from August 1994 to March 1992, at the out patient clinin of Department of Dermatology of Yonsei University. 252 patients who were treated with SPTL was analyzed to evaluate the clinical effects of the pulsed dye laser. Patient di@st.ributition f the cutaneous vascular lesions included 1fil pat.ients with nevui flammeus, 68 with telangcetasia, 15 with spider angima, 5 with strawberry hemangiona and 2 with cavernous hemangior ia. RESULTS: The results and summarized as follows : 1. Nevus flammeus 4b.7% of early childhood, 41.5% of late childhood and 47.3% of adults showed excellent or good results. 2. Telangiectasia 78.1% of patients she wed excellent or good results. 3. Spider angioma 66.7% of patients she wed excellent or good results. 4. Strawberry hemangioma and cavernous hemangioma Most, of the patients,howed fair or poor results. 5. Complications included hyperpigmentation(26.5%), hypopigmentation(1.6%) and atrophic scar(4.5% ). CONCLUSION: Our experince with pulsed dye laser during the last 3 years revealed that it was superior in treating vascular disease than other existing lasers.
Adult
;
Dermatology
;
Epidermis
;
Fragaria
;
Hemangioma
;
Hemangioma, Cavernous
;
Humans
;
Lasers, Dye*
;
Port-Wine Stain
;
Spiders
;
Telangiectasis
;
Vascular Diseases
6.Urachal cyst presenting with huge abscess formation in adults.
Sung Hwan LEE ; Hyang Im LEE ; Dong Gue SHIN
Journal of the Korean Surgical Society 2012;83(4):254-257
Urachal disease, a disorder where embryonic remnant of the cloaca and the allantois present after birth as a midline fibrous cord, is usually detected in infancy and childhood. But urachal disease in adults is rare. We report a case of a huge abscess derived from a urachal cyst in an adult. A 52-year-old man presented with peri-umbilical distension and abdominal pain for 2 weeks. Ultrasonography and abdominal computed tomography scan demonstrated a huge abscess derived from the abdominal wall. After prompt incision and drainage, the remaining abscess cavity was removed completely under general anesthesia. Pathologic report was consistent with urachal duct cyst, and the patient was discharged in a week without complication.
Abdominal Pain
;
Abdominal Wall
;
Abscess
;
Adult
;
Allantois
;
Anesthesia, General
;
Cloaca
;
Drainage
;
Humans
;
Middle Aged
;
Parturition
;
Urachal Cyst
7.Electron Microscopic Study of the Renal Proximal and Distal Convuluted Tubular Epithelial and Distal Convoluted Tubular Epithelial Cells after Administration of Aspirin.
Sung Gum HONG ; Cheul Woon BACK ; Im Ju KANG ; Byung Heon KIM ; Dong Koo LEE
Journal of the Korean Pediatric Society 1983;26(6):564-572
No abstract available.
Aspirin*
;
Epithelial Cells*
8.Effect of Panax Ginseng Extracts on the Experimentally Induced Comedones.
Sung Bin IM ; Eung Ho CHOI ; Dong Sik BANG ; Soo Il CHUN ; Jung Bock LEE
Korean Journal of Dermatology 1990;28(4):434-440
In this animal study, comedones were induced by topical application of oleic acid onto the rabbit ear canal and the histopathologic and ultrastructural changes in response to topical application of HO and hexane extracts of red ginseng, and saponin were measured. No microscopical difference was observed between treatment groups. Nucle ir remnants were observed in the horny cell layer as well as the thinning of the granular cell layer. Scattered and decreased keratohyaline granules were also noted. Six weeks after initiation of treatment, we observed the thinning of epidermis and veappearance of granular cell layer with normal keratohyaline granules. After treatment, scanning electron microscopy showed a decrease in the size of comedones, and loosened and desquamating horny cells in the comedones. From the above results, it could be concluded that Panax ginseng extracts might have effects on the keratinization process of the skin.
Animals
;
Ear Canal
;
Epidermis
;
Microscopy, Electron, Scanning
;
Oleic Acid
;
Panax*
;
Saponins
;
Skin
9.A Case of Multiple Nevus Sebaceus.
Dae Hwan KIM ; Soo Young LEE ; Sung Bin IM
Korean Journal of Dermatology 2001;39(5):604-606
The nevus sebaceus is an organoid nevus generally noted on the scalp or the face as a single lesion. The frequent association of nevus sebaceus with other appendage tumors and with apocrine glands suggests that nevus sebaceus is derived from the primary epithelial germ. We report a 20-day-old boy with multiple nevus sebaceus on his face, scalp and external ear. The lesions were symmetrical and midline involved yellowish plaques. On the microscopic view of biopsy specimen from the face and scalp, there is a dilated keratin-filled infundibula showing a few buds of undifferentiated cells representing malformed hair germs. The dermis contains many fibroblasts and immature hair structures. Numerous sebaceous glands and eccrine glands lie in upper dermis. Diagnosis was confirmed by clinical appearance and histological picture.
Apocrine Glands
;
Biopsy
;
Dermis
;
Diagnosis
;
Ear, External
;
Eccrine Glands
;
Fibroblasts
;
Hair
;
Humans
;
Male
;
Nevus*
;
Organoids
;
Scalp
;
Sebaceous Glands
10.Comparison of the 3-Imaging Procedures (IVP,VCUG & USG) on Children with Urinary Tract Infection.
Min Hee YEO ; Won Yeob KIM ; Jee Sung KIM ; Sang Geel LEE ; Im Ju KANG
Journal of the Korean Pediatric Society 1990;33(3):335-341
No abstract available.
Child*
;
Humans
;
Urinary Tract Infections*
;
Urinary Tract*