Keratoacanthoma is a benign neoplasm that develops as a protruding mass especially on the central part of face, We experienced a case of keratoacanthoma that developed on the nasal vestibule after the eradication of verruca vulgaris on the same site by surgical intervention 3 months before. We treated it with total excision and skin graft.
Keratoacanthoma* ; Skin ; Transplants ; Warts*

To evaluate the effects of Dexamethasone(Dex), Platelet derived growth factor-BB(PDGF) and combination of Dex and PDGF(DP) on the growth and differentiation of MC3T3-E1 cells, Dex(10(-7) M) and PDGF(10 ng/ml) in experimental group were added to the cells at the days 5, 10, 15, 20, 25 and examined for cell proliferation activities, DNA synthesis activities, ALP activities and bone nodule formation. The results were as follows : 1.In Dex group, cell proliferation, DNA synthesis and ALP activities were lower until 15 days when compared to the control group. Bone nodules formation were shown at 10 days. 2.In PDGF group, cell proliferation and DNA synthesis activities were higher until 15 days and ALP activities were lower when compared to the control and Dex groups. Bone nodules formation were shown at 20 days. 3.In DP group, cell proliferation and DNA synthesis activities of PDGF were suppressed by Dex and synergistic effects of combination of Dex and PDGF on ALP activities were shown at days 5 when compared to control and Dex groups. Bone nodules formation activities of Dex were suppressed by PDGF.
Blood Platelets ; Cell Proliferation ; Dexamethasone* ; DNA

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA ; Genetics, Population ; Humans ; Immunoglobulin Allotypes* ; Immunoglobulins* ; Lymphocytes ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

It is well known that Bowen's.disease, a precancerous dermatosis can be associated with internal malignancy. We report 2 cases of Bowen's disease of probable arsenic origin associated with. internal malignancies. Case one, a 55 year-old man, who had the past history of arsenic intake and herb medication for the treatment of suspicious leprosy, had Bowen's disease and arsenical keratosis on his palms and soles with bronchogenic and stomach carcinoma. The other case, a 65 year-old man, who had history of intake of arsenic and herb medicine for epilepsy, had Bowen's disease and squamous cell carcinoma of the skin with stomach cancer. We suggest that in these 2 cases, the ingestion of organic arsenics might be the causative factor for the internal malignancy.
Aged ; Arsenic ; Bowen's Disease* ; Carcinoma, Squamous Cell ; Eating ; Epilepsy ; Humans ; Keratosis ; Leprosy ; Middle Aged ; Skin ; Skin Diseases ; Stomach ; Stomach Neoplasms

The nail-patella syndrome is a genetically determined autosomal dominant disease with variabIe expression affectiag tissues of both ectodermal and mesodermal origin. The condition in an individual is characterized chiefly by dysplasia of the fingernails, hypoplastic or absent patellae, deformities or luxation af the head of the radius, and the presence of illiac horns. It is now well established that the locus for the nail-patella syndrome and the locus for the ABO blood groups are closely linked. We report 2 cases of nail-patella syndrome affected a brother and a sister in a family. Our 2 cases, with blood group B, showed dystrophic nails and hypoplastir. patellae.
Animals ; Blood Group Antigens ; Congenital Abnormalities ; Ectoderm ; Head ; Horns ; Humans ; Mesoderm ; Nail-Patella Syndrome ; Nails ; Patella* ; Radius ; Siblings

OBJECTIVE: Linkage analysis is a very useful method for prenatal diagnosis of Hemophilia B, especially when a mutation was not identified. Seven polymorphic markers were studied in Korean populations to evaluate the efficiency for prenatal and carrier diagnosis. METHODS: Subjects of this study was 100 healthy Korean women (200 X-chromosomes). Polymerase chain reacton-restriction fragment length polymorphism (PCR-RFLP) method was used to detect SalI, MseI, NruI, DdeI, XmnI, TaqI and HhaI polymorphisms. RESULTS: SalI (-) allele showed the frequency of 0.355 and SalI(+) allele 0.645. MseI(-) allele was 0.645 in frequency and MseI(+) allele was 0.355. SalI and MseI polymorphisms were in complete linkage disequilibrium. And no increase was expected in overall heterozygosity with these two polymorphisms. NruI(-) allele frequency was 0.855 and NruI(+) was 0.145. There was no polymorphism of DdeI, XmnI and TaqI marker systems in Korean population. In HhaI polymorphism, allele frequencies were estimated that HhaI(-) is 0.82 and HhaI(+) is 0.18. CONCLUSION: Only SalI, NruI and HhaI polymorphisms are useful for the diagnosis of hemophilia B in Korean population. Expected heterozygosity for above 3 poylmorphic markers was estimated to be 0.723, and 71 of 100 female subjects were heterozygous for at least one marker system. Korean population showed relatively low extent of polymorphisms compared to Caucasians, Blacks and Japanese. For the effective prenatal diagnosis of hemophilia B with linkage analysis, other polymorphic markers should be evaluated.
African Continental Ancestry Group ; Alleles ; Asian Continental Ancestry Group ; Diagnosis ; Factor IX* ; Female ; Gene Frequency ; Hemophilia B ; Humans ; Linkage Disequilibrium ; Prenatal Diagnosis

Penile Horn occurs as a single or multiple lesions. It usually starts as warty growth that later becomes hyperkeratotic and assumes the appearance of a horn. A 22-year-old male patient was presented with a slow growing, asymptomatic, dark brownish colored, corn-shaped, protruding mass on prepuce of the penis for 5 months. Histopathologic finding showed verruca vulgaris. We think that penile horn in this case may be come from verruca vulgaris.
Animals ; Horns* ; Humans ; Male ; Penis ; Warts ; Young Adult

The effects of epidural bupivacaine with and without epinephrine and morphine on mat-ernal blood pressure, newborn Apgar scores, and duration of analgesia were compared in 40 parturients during Cesarean section and post operative periods. Patients in group 1(n= 16) received 0.5% bupivacaine 27cc and group 2(n=10) recelved bupivacaine with epinephrine, group 3 (n= 10) received bupivacaine with morphine 3mg, and those in group 4(n= 10) received bupivacaine with epinephrine and morphine 3mg. Maternal hypotension occured less frequently in group 2 than in group 1 (p<0.001). Apgar scores were equally good (more than 8) all four groups. Duration of analgesia was longer in group 2(199.50+/-70.73min) than in group 1(133.50+/-50. 11min) but significantly longer in group 3(471.50+/-174.90) and in group 4(684.00+/-276. 92min) . It is concluded that adding epinephrine and morphine to bupivacaine during epidural anesthesia in the normal parturient has no adverse effects on either mother or neonate; and that it significantly prolongs the duration of analgesia and decreases the incidence of maternal hypotension.
Analgesia ; Anesthesia, Epidural* ; Blood Pressure ; Bupivacaine* ; Cesarean Section ; Epinephrine* ; Female ; Humans ; Hypotension ; Incidence ; Infant, Newborn ; Morphine* ; Mothers ; Obstetrics* ; Pregnancy

BACKGROUND: Recently, a lower incidence of late potentials has been reported in patients with acute myocardial infarction after successful thrombolysis when compared with conventionally treated patients. In another recent study, however, no significant effect of thrombolytic therapy on any abnormal signal average electrocardiography was found at 13 days after acute myocardial infarction. The present study was designed to determine the prognostic significance of the signal average electrocardiography and to evaluate the possible value of this technique as a noninvasive tool for monitoring of coronary occlusion and reperfusion. METHODS: Signal averaging was performed by using a signal average electrocardiography with bidirectional filterings before coronary artery occlusion, at 5 minutes after coronary occlusion and on reperfusion in 20 cats. Three of them died due to malignant ventricular arrhythmia during reperfusion. In all cats, approximately 250 beats were averaged. All data were analysed at filter frequency 25 to 250Hz, 40 to 250Hz and 80 to 250Hz. The following quantitative high resolutional electrocardiographic variables were calculated by computer : 1) filtered total QRS duration, 2) duration of HFLA(high frequency low amplitude) signals under 40uV, 3) RMS voltage of terminal 40ms, 4) mean Voltage of terminal 40ms, 5) average noise voltage. RESULTS: At the filter frequency of 40 to 250Hz and 80 to 250Hz, the filtered QRS duration and duration of HFLA signals 40uV were significantly prolonged at 5 minutes after coronary artery occlusion than before coronary occlusion(p<0.01). At the filter frequency of 40 to 250Hz and 80 to 250Hz, the RMS voltage(terminal 40ms) and mean voltage(terminal 40ms) were significantly prolonged at 5 minutes after coronary artery occlusion than before coronary occlusion(p<0.01, p<0.01 respectively). At the filter frequency of 80 to 250Hz, the filtered QRS duration and at the filter frequency of 25-250Hz, the duration of HFLA signals at 40uV were significantly shortened during reperfusion than at 5 minutes after coronary artery occlusion(p<0.01, p<0.05 respectively). At the filter frequency of 40 to 250Hz and 80 to 250Hz, the RMS voltage(terminal 40ms) and mean Voltage(terminal 40ms) were significantly shortened during reperfusion than at 5 minutes after coronary artery occlusion(p<0.01, p<0.01 respectively). There was no significant change of the filtered QRS duration, duration of HFLA signals 40uV,RMS voltage(terminal 40ms) and mean Voltage(terminal 40ms) after reperfusion compared with those of control at the filter frequency of 25 to 250Hz, 40 to 250Hz and 80 to 250Hz respectively. CONCLUSION: These results suggest that the signal average electrocardiography could be a valuable tool for monitoring the state of coronary artery occlusion and reperfusion.
Animals ; Arrhythmias, Cardiac ; Cats* ; Coronary Occlusion ; Coronary Vessels* ; Electrocardiography* ; Humans ; Incidence ; Myocardial Infarction ; Noise ; Reperfusion* ; Thrombolytic Therapy

Antigen-specific T cell clones were obtained from mice immunized with Fusobacterium nucleatum ATCC 10953(F. nucleatum) and/or Porphyromonas gingivalis 381(P. gingivalis). 10 Balb/c mice per group were immunized with F. nucleatum followed by P. gingivalis, or with P. gingivalis alone by intraperitoneal injection of viable microorganisms. Spleen T cells were isolated and stimulated in vitro with viable P. gingivalis cells to establish P. gingivalis-specific T cell clones. T cell phenotypes and cytokine profiles were determined along with T cell responsiveness to F. nucleatum or P. gingivalis. Serum IgG antibody titers to F. nucleatum or P. gingivalis were also determined by ELISA. All the T cell clones derived from mice immunized with F. nucleatum followed by P. gingivalis demonstrated Th2 subsets, while those from mice immunized with P. gingivalis alone demonstrated Th1 subsets based on the flow cytometric analysis and cytokine profiles, All T cells clones from both groups were cross-reactive to both P. gingivalis and F. nucleatum antigens. Phenotypes of T cell clones were all positive for CD4. Mean post-immune serum IgG antibody levels to F. nucleatum or P. gingivalis were significantly higher than the preimmune levels(p<0.01, respectively). There were no significant differences in the antibody titers between the two groups. It was concluded that P. gingivalis-specific T cells initially primed by cross-reactive F. nucleatum antigens were polarized to Th2 subsets, while T cells stimulated with P. gingivalis alone maintained the profile of Th1 subset.
Animals* ; Clone Cells ; Enzyme-Linked Immunosorbent Assay ; Fusobacterium nucleatum ; Immunity, Humoral* ; Immunization* ; Immunoglobulin G ; Injections, Intraperitoneal ; Mice ; Models, Animal* ; Phenotype ; Porphyromonas gingivalis ; Spleen ; T-Lymphocytes