PURPOSE: The purpose of this study was to determine whether Fas-L expression is associated with increased apoptotic induction of tumor-infiltrating lymphocytes (TIL) in human gastric carcinomas. MATERIALS AND METHODS: The author analysed 38 cases of early gastric carcinoma (EGC) and 61 cases of advanced gastric carcinoma (AGC) who received gastric resection, in whom the number of diffuse type was 38 cases and the number of intestinal type was 61 cases. The author used immunohistochemical staining for Fas, Fas-L and CD45, and TUNEL in situ apoptosis detection kit. TIL were detected by CD45 and apoptosis of TIL were detected by CD45 expression and TUNEL positivity on serial histologic sections. RESULTS: Fas-L was localized to neoplastic cells in 61% (23/38) of EGC group and 66% (40/61) of AGC group. The extent of Fas-L expression was variable, with both Fas-L positive and negative neoplastic region occuring within tumors. TIL adjacent to Fas-L expressing tumor region were decreased in number and TIL adjacent to FasL-negative tumor region were increased in number; apoptotic induction of TIL showed just the opposite pattern (p<0.05). Fas expression was found essentially homogeneously throughout the tumor mass independent of tumor stage. Fas expression showed 64% (39/61) of intestinal type and 68% (26/38) of diffuse type. Labeling indices for tumoral apoptosis in EGC and AGC were 6.72% and 7.13%, respectively and this difference was statistically insignificant. Co-expression of Fas-L and Fas, which occurred over large areas of the tumors, did not result in an enhanced rate of tumor cell apoptosis. In addition, factors such as tumor stage and other prognostic factors were not concerned in Fas and Fas-L expression, number of TIL and apoptotic induction. CONCLUSION: These findings suggest Fas-mediated apoptotic depletion of TIL in response to Fas-L expression by stomach cancers, and provide the evidence to support the Fas counterattack as a mechanism of immune escape in gastric cancer. In addition, gastric carcinoma cells of the intestinal and diffuse type did not differ in their expression of the apoptotic receptor Fas.
Apoptosis* ; Humans ; In Situ Nick-End Labeling ; Lymphocytes, Tumor-Infiltrating ; Stomach Neoplasms ; United Nations

With development of immunochemical methods for anlysis of serum proteins, quantitative determination of serum immunoglobulin levels and purification of them have been made possible in recent years. There are numerous reports about immunoglobulin levels in various diseases. A search of the literature reveals very few reports of quantitative immunoglobulin determinations in allergic dermatoses. The present study was conducted to find quantitative changes of serum immunoglobulins in a few allergic dermatoses. To quantify the IgG, IgA and IgM, one hundred and two sera were analysed form 30 normal control group, 30 contact dermatitis patients, 12 stopic dermatitis patients and 30 urticaria patients by modified Oudin's capillary tube method. The results are as follows. 1.In contact dermatitis the IgG was significantly increased to compare with control group(p<0.01) 2.In atopic dermatitis the IgA was significantly increased to compare with control group(p<0.01) 3.In urticaria the IgG was increased (p<0.05) 4.The IgM has no differences between allergic dermatoses and control group.
Blood Proteins ; Capillaries ; Dermatitis ; Dermatitis, Atopic ; Dermatitis, Contact ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Immunoglobulins ; Skin Diseases* ; Urticaria

The proteins, especially the 19S globulin (IgM) and 7S globulin (IgA) in the CO2 snow-induced blister fluids of 10 male healty volunteers were analyzed immunoelectrophoretically. The fnllawing results were obtained. 1) Compared with autologous serum, all blister fluids tested lacked some protein fraction lines. 2) The 19S globulin (lgM) line was absent in all of the blister fluids except one which was contaminated with blood. 3) The 7S globulin (IgA) line was present in all of the blister fluids when undiluted. In 1 : 2 dilution, however, it was absent, or of trace amount in a few cases.
Blister* ; Humans ; Male ; Volunteers

The purpose of this study is to compared and analyze the shade changes(deltaE)about In-Ceram(R),IPS-Empress(R),OPC(R) by using of the spectrophotometer arising from initial status and before and after cementation of the resin cement mounted on the metal core. We used a couple of statistics such as 'One-Way ANOVA'and Multiple Range Test. We could be able to verify significantly what is being discussed here up to 95%. The results drawn from our research are as follows: 1. At the time of our experiments regarding the initial shapes of all-ceramic and mounting status of all-ceramic on the metal crown(deltaE1), and a comparison of mounting of all ceramic on the metal crown with all-ceramic cemented on the metal core(deltaE2), at the time of shade change of initial shapes and after we cemented on the metal core. (1) no significant difference among all-ceramics was found. (2) no particular difference was found regarding the In-Ceram(R) (3) a significant difference between the deltaE1 and deltaE2 regarding the IPSS-Empress(R) was found (P<0.05). (4) a significant difference between the deltaE1 and deltaE2 regarding the OPC? was found (P<0.05). 2. When we compared the shade changes (deltaE) resulted from before and after the cementation on each of the parts involved of some all-ceramic . we could be able to find shape change increase from incisal third, middle third, and cervical third in that order in In-Cream(R)(spinell), IPS Empress(R) and OPC(R) all. In addition , we could be able to find a siginificant difference between cervical third and incisal third, middle third. (P<0.05) From what we have just seen, we might conclude that there is a significant shade change difference before and after the cementation with respect to IPS-Empress(R)and OPC(R). In addition, we could also be able to find more shade change difference at the cervical third rather than incisal third and middle third depending on the parts involved.
Cementation ; Ceramics* ; Crowns* ; Resin Cements

No abstract available.
Frontal Sinus*

PURPOSE: We evaluated the correlation between the carcinogenesis of gallbladder and the expression of lysozyme, p53, c-erbB2 and CEA in gallbladder lesions. MATERIALS AND METHODS: Thirty cases of gallbladder lesions (containing 17 cases of GB carcinoma) were examined. We analyzed the clinicopathologic findings of the early (stage I & II) and advanced carcinoma (stage III, IV & V) and those of carcinoma with or without metaplasia in the tumor. We performed p53, c-erbB2 and CEA immunohistochemical staining and compared their findings with those of normal mucosa and preneoplastic lesions. We also performed lysozyme immunohistochemical staining and compared its finding with metaplastic and non-metaplastic lesions. RESULTS: There are two distinct genetic pathways in gallbladder cacinogenesis and metaplastic carcinoma was more frequent than non-metaplastic carcinoma. Metaplasia of gallbladder did not reveal any difference of the clinicopathologic findings and depth of invasion (Nevin stage). Lysozyme expression was found in all metaplastic lesions but non-expression did not indicate non-metaplastic lesions. p53 mutations and c-erbB2 alterations may have a role in the carcinogenesis of gallbladder carcinomas, especially, in a late event, and in an early and late events, respectively. The correlation of p53 and c-erbB2 expressions was found but which did not indicate that the co-expression was needed in the carcinogenesis. CEA immunohistochemical staining may be helpful in the differential diagnosis of benign lesions and precancerous and cancerous lesions of the gallbladder. CONCLUSION: These results suggest that p53 mutations and c-erbB2 alterations may have a role in the carcinogenesis of gallbladder carcinomas, especially, in a late event, and in an early and late events, respectively.
Carcinogenesis ; Diagnosis, Differential ; Gallbladder* ; Metaplasia* ; Mucous Membrane ; Muramidase

The 17 patients with 22 resistant clubfeet were treated with modified Turco's operation with or without combining release of calcaneocuboid joint at Department of Orthopaedic Surgery, Hanyang University School of Medicine between 1896 and 1991. They were analysed preoperatively and postoperatively with chinical and radiological methods. In patients who were treated with combined procedures, the latetal talocalcaneal angle and anteroposterior talo-first metatarsal angle in radiological findings were showed better improvement(p < 0.05), and anteroposterior talocalcaneal angle was also showed better improvement but not significant statistically, than those who were treated with modified Turco's operation alone. The final results of combined procedures were better than that of modified Turco's operation. And the most common characteristic physical findings in resistant clubfoot were small heel, deep longitudinal crease on the medial aspect of midfoot area and a single transverse crease on the posterior heel just proximal to the insertion of the tendocalcaneus. So we suggest these physical findings may be expectance for the resistance of the congenital clubfoot to conservative treatment, but it need further comparative study.
Clubfoot ; Heel ; Humans ; Joints ; Metatarsal Bones

Recently as the esthetic concerns about dental prosthesis have been increased, the nonmetallic post and core systems have been introduced clinically to improve the color and the low optical transmittance of conventional casting metal post and core systems. The purpose of this study was to compare and analyze the optical transmittance and the shade changes of all-ceramic crowns with two nonmetallic post and core systems. The experimental groups were classified as follows: Total 27 specimens(9 samples in each group) were evaluated. Group I: Natural teeth as a control group. Group II: CosmoPost and Empress(R) core (Ivoclar-Vivadent, Liechtenstein). Group III: LIGHT-POST(TM) and LIGHT-CORE(TM)(Bisco, U.S.A.). In all groups, all-ceramic crowns were fabricated with the same shade of IPS Empress(R)II(Ivoclar-Vivadent, Liechtenstein) after abutment preparation, and then two kinds of spectrophotometers, UV 3101 PC(Shimadzu,Japan) and CM 503i(Minolta Co., Ltd., Japan), were used to measure the optical transmittance and the color value. The following results were obtained: 1. The optical transmittance of each group without all-ceramic crowns, was siginificantly decreased in order of Group III, I and II(p<0.05). 2. The optical transmittance of each group with all-ceramic crowns cemented, were siginificantly decreased in order of Group I, II and III(p<0.05). 3. In comparison with the shade of all-ceramic crowns, there were no significant differences between groups regardless of the cementation. 4. In comparison with the shade changes in each group after the cementation of all-ceramic crowns, there were no significant differences between groups. From the above results, it is thought that the selection among materials used in this study doesn't influence greatly the esthetic restoration, because the differences of optical transmittance were so small that those were clinically insignificant.
Cementation ; Crowns* ; Dental Prosthesis ; Tooth

No abstract available.
Diagnosis* ; Humans ; Thoracic Injuries* ; Thorax* ; Tomography, X-Ray Computed*

PURPOSE: Diffuse panbronchiolitis(DPB) is a chronic inflammatory airway disease of unknown causes mainly affecting the respiratory bronchioles and the more proximal bronchi. Findings on chest radiographs and high-resolution CT(HRCT) are well known and Akira classified HRCT findings of DPB into four types. Purpose of this study is to evaluate the relationship between findings of HRCT and PFT. METHODS AND MATERIALS: We retrospectively analyzed the chest radiographs and HRCT images of eleven patients with DPB and compared CT classification with pulmonary function test. RESULTS: Chest radiographs usually showed small nodular opacities throughout the both middle and lower lungs. The HRCT findings of DPB were centrilobularly distributed small nodular densities, branching linear densities contiguous with small round densities, dilated and thickened peripheral and central airways including bronchioles. More than one CT type by Akira's classification, usually two or three types, were found in nine patients. There was good correlation between CT types and FEV1%(p<0.05), CT types and FEV1/FVC (p<0.05) respectively. CONCLUSION: HRCT seems to be more useful for diagnosis and disease progression of DPB as compared with the chest radiographs or clinical stage such as pulmonary function test.
Bronchi ; Bronchioles ; Classification ; Diagnosis ; Disease Progression ; Humans ; Lung ; Radiography, Thoracic ; Respiratory Function Tests* ; Retrospective Studies