The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA ; Genetics, Population ; Humans ; Immunoglobulin Allotypes* ; Immunoglobulins* ; Lymphocytes ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

No abstract available.
Porokeratosis

No abstract available.
Humans* ; Intercellular Adhesion Molecule-1* ; Lung Neoplasms* ; Lung*

No abstract available.
Tattooing ; Warts

No abstract available.
Animals ; Horns* ; Keratoderma, Palmoplantar*

No abstract available.
Capillaries* ; Hemangioma, Capillary*

No abstract available.
Cystitis*

OBJECTIVE: This study was designated to determine the effect of cord blood cell transplantation in ischemic injury model. METHODS: In this study, we administered human umbilical cord blood (hUCB)-derived CD34(+) cells into the lateral ventricle or directly into the striatum and assessed cell migration in mice with cryoinjury and behavioral recovery in rats with transient middle cerebral artery occlusion (MCAo). CD34(+) cells were isolated by magnetic cell sorting using CD34-microbeads and labeled with CM-Dil. RESULTS: When CD34(+) cells were injected into mice brain with cryoinjury, cells were migrated into a injury site after one week of injection. Similarly, injected CD34(+) cells were migrated into the periphery of infarcted area in rats with transient MCAo. When spontaneous activity was measured using a modified neurological severity score (mNSS), it was found that functional recovery was significantly higher when CD34(+) human umbilical cord blood cell (hUCBC) was transplanted 24 hours after stroke compared with phosphate buffered saline (PBS)-injected or CD34(-) transplanted, stroked animals (P<0.05). Although only small portion of transplanted cells were differentiated into neural lineages, CD34(+) hUCBC transplantation increased Brdu incorporation and recruitment of doublecortin (DCX) (+) cells in ischemic boundary zone. CONCLUSION: These results suggest that hUCBC transplantation may be an effective treatment for brain injuries, such as stroke, or neurodegenerative disorders by promoting endogenous repair process of the brain.
Animals ; Brain ; Brain Injuries ; Bromodeoxyuridine ; Cell Movement ; Cell Transplantation ; Fetal Blood ; Humans ; Infarction, Middle Cerebral Artery* ; Lateral Ventricles ; Mice ; Middle Cerebral Artery* ; Neurodegenerative Diseases ; Rats* ; Stroke ; Transplants

BACKGROUND/AIMS: Partial hepatectomy is considered as best treatment for selected HCC patients combined with liver cirrhosis. We conducted this study to assess the safety and effects of partial hepatectomy for HCC combined with liver cirrhosis and to identify the prognostic factors. METHODS: Retrospective analysis was performed for 115 HCC patients with liver cirrhosis who underwent hepatectomy in Korea Cancer Center Hospital from 1987 September to 2002 July. Median follow-up period was 30 months. RESULTS: In-hospital mortality rate was 0.87% (1/115 cases). 5-years overall and disease free survival rates were 59.3% and 44.75%, respectively. Fifty three patients developed recurrence and most common recurrent site was remnant liver (81.1%) and 3-year survival rate after recurrence was 33.4%. Independent prognostic factors for overall survival rate were TNM stage, multiplicity and venous invasion (p<0.05). CONCLUSION: Hepatectomy plays a significant role for HCC with compensated liver cirrhosis and is regarded as the effective treatment with acceptable mortality. However, recurrence rate is still high, so frequent follow-up study should be needed for early detection of recurrence amenable to effective treatment. For the high risk patients, further study should be followed to reduce the recurrence after hepatectomy, including surgical technique and adjuvant treatment.
Carcinoma, Hepatocellular* ; Disease-Free Survival ; Follow-Up Studies ; Hepatectomy* ; Hospital Mortality ; Humans ; Korea ; Liver Cirrhosis* ; Liver* ; Mortality ; Recurrence ; Retrospective Studies ; Survival Rate

BACKGROUND: The role of stratum corneum has been known to be the major barrier against percutaneous absorption. The change of osmotic gradient onto stratum corneum may affect the permeability barrier function though its mechanism has not been explained. OBJECTIVE: In this study, we have tested hyperosmolar glucose solution (1M, 0.1M) over the living skin or the separated epidermal sheets to determine the penetration-related pharmacokinetics such as absorption, saturation, diffusion kinetics in vivo or in vitro. METHODS: The hyperosmolar glucose patches were applied to 10 healthy volunteers' forearm skin to analyze the absorption profiles through stratum corneum. For investigating the role of osmotic pressure influencing the disposition of glucose, in vitro two compartment model was used to characterize the pharmacokinetics of glucose through epidermal sheets. RESULTS: The quantitative assay of applied hyperosmolar glucose from sequentially stripped stratum corneum of volunteers revealed the high glucose/protein ratio and steep concentration gradient at the uppermost layers down to lower layers. The pharmacokinetic profile of hyperosmolar glucose in vitro shows both the saturation delay pattern and steady flux pattern regarding glucose diffusion. CONCLUSION: The stratum corneum act as a major permeation barrier against glucose disposition, though the concentration-dependent pharmacokinetics by its osmotic gradient were rather different. Thus, the osmolarity-related event over stratum corneum might be a considerable factor during percutaneous absorption.
Absorption ; Diffusion ; Forearm ; Glucose* ; Kinetics ; Osmotic Pressure ; Permeability ; Pharmacokinetics* ; Skin ; Skin Absorption ; Volunteers