1.Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients.
Jeom Il CHOI ; Sung Jo KIM ; In Hoo KIM
The Journal of the Korean Academy of Periodontology 1999;29(2):349-354
The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA
;
Genetics, Population
;
Humans
;
Immunoglobulin Allotypes*
;
Immunoglobulins*
;
Lymphocytes
;
Polymerase Chain Reaction*
;
Polymorphism, Restriction Fragment Length
2.Porokeratosis Ptychotropica: A Lesser-known Variant of Porokeratosis
Hoo Min CHOI ; Sung Min KIM ; Jae Won KANG ; Byung In RO ; Han Kyoung CHO
Korean Journal of Dermatology 2019;57(9):562-563
No abstract available.
Porokeratosis
3.Expression of intercellular adhesion molecule-1 in human primary lung cancers.
Young Chun CHOI ; Dong Ho SHIN ; Sung Soo PARK ; Dong Hoo LEE ; Jung Hee LEE ; Jung Dal LEE
Korean Journal of Medicine 1993;45(4):467-472
No abstract available.
Humans*
;
Intercellular Adhesion Molecule-1*
;
Lung Neoplasms*
;
Lung*
4.A Case of Palmoplantar Keratoderma Presenting as Multiple Cutaneous Horns.
Hoo Min CHOI ; Jong Baik KIM ; Suk Young LEE ; Sung Min KIM ; Eun Jung KO ; Byung In RO ; Han Kyoung CHO
Korean Journal of Dermatology 2018;56(9):562-564
No abstract available.
Animals
;
Horns*
;
Keratoderma, Palmoplantar*
5.A Case of Superficial Spreading Capillary Hemangioma.
Suk young LEE ; Jong Baik KIM ; Hoo Min CHOI ; Sung Min KIM ; Eun Jung KO ; Byung In RO ; Han Kyoung CHO
Korean Journal of Dermatology 2018;56(6):396-397
No abstract available.
Capillaries*
;
Hemangioma, Capillary*
6.Verruca Plana Developed after Tattooing
Sung Min KIM ; Jong Baik KIM ; Hoo Min CHOI ; Byung In RO ; Han Kyoung CHO ; Eun Jung KO
Korean Journal of Dermatology 2019;57(3):168-169
7.Two Cases of Emphysematous Cystitis.
Joo Ik PARK ; Joo Myeong SHIM ; Seong Yoon JUNG ; Young Hoo SEO ; Jae Il JUNG ; Ho Cheol CHOI ; Sung Hyup CHOI ; Heon Sung LEE
Korean Journal of Urology 2000;41(8):1033-1095
No abstract available.
Cystitis*
8.Evaluation of Seeplex(TM) RV Detection Kit for Detecting Rhinovirus, Human Metapneumovirus, and Coronavirus.
Heungsup SUNG ; Sook Ja PARK ; Young Dae WOO ; Byung Hoo CHOI ; Mi Na KIM
The Korean Journal of Laboratory Medicine 2008;28(2):109-117
BACKGROUND: Direct antigen test (DAT) and culture are primary tests to diagnose infections by respiratory viruses, but are mainly available for the traditional viral pathogens such as respiratory syncytial virus (RSV), influenza virus, parainfluenza virus (PIV), and adenovirus in clinical laboratories. The objective of this study was to evaluate a multiplex reverse transcriptase-PCR method using Seeplex(TM) RV Detection kit (Seegene, Korea) for the detection of rhinovirus, coronavirus, and human metapneumovirus (hMPV). METHODS: From January to May 2007, nasopharyngeal aspirates (NPAs) from pediatric patients negative for culture and DAT of traditional viral pathogens were tested with Seeplex(TM). All the amplicons were directly sequenced and homology of the sequences was searched in the National Center for Biotechnology Information (NCBI) database. Patients' medical records were reviewed for clinical and demographic features. RESULTS: Forty-seven (26.4%) of 178 NPAs were positive: 18 rhinovirus, 15 hMPV, 4 RSV A, 3 coronavirus OC43, 3 influenza virus A, 2 adenovirus, 1 coronavirus NL63, and 1 RSV B. Based on maximum identity, each of the sequences indicating rhinovirus, hMPV, and coronavirus OC43 matched to the corresponding viruses with homology of 94-98%, 96-99%, and 98-100%, respectively. Seeplex(TM) positive patients were 0-11 yr old with a male:female ratio of 1.5:1. Clinical diagnoses included 9 pneumonia, 6 bronchiolitis, 2 cold, 1 asthma exacerbation for rhinovirus; 10 pneumonia, 4 bronchiolitis, and 1 clinical sepsis for hPMV; and 1 pneumonia, 2 croup, and 1 cold for coronavirus. CONCLUSIONS: Multiplex reverse transcriptase-PCR method using Seeplex(TM) RV Detection kit is a reliable test to detect rhinovirus, hMPV, and coronavirus. It may improve the diagnostic sensitivity for RSV, influenza virus, PIV, and adenovirus.
Adolescent
;
Child
;
Child, Preschool
;
Coronavirus/classification/*isolation & purification
;
Coronavirus 229E, Human/classification/genetics/isolation & purification
;
Coronavirus OC43, Human/classification/genetics/isolation & purification
;
Female
;
Humans
;
Infant
;
Infant, Newborn
;
Male
;
Metapneumovirus/classification/genetics/*isolation & purification
;
Phylogeny
;
Reagent Kits, Diagnostic
;
Respiratory Tract Infections/*diagnosis/virology
;
Reverse Transcriptase Polymerase Chain Reaction/*methods
;
Rhinovirus/classification/genetics/*isolation & purification
;
Sequence Analysis, DNA
9.Quantitation of BK Virus DNA for Diagnosis of BK Virus-Associated Nephropathy in Renal Transplant Recipients.
Heungsup SUNG ; Byung Hoo CHOI ; Yeon Jung PYO ; Mi Na KIM ; Duck Jong HAN
Journal of Korean Medical Science 2008;23(5):814-818
Quantitative measurement of BK virus DNA (Q-BKDNA) has been used for the early diagnosis and monitoring of BK virus-associated nephropathy (BKVAN). This study was designed to determine the BKDNA cutoff for the diagnosis of BKVAN. Between June 2005 and February 2007, 64 renal transplant recipients taken renal biopsies due to renal impairment submitted plasma and urine for Q-BKDNA. Eight BKVAN patients (12.5%) had median viral loads of 6.0 log(10) copies/mL in plasma and 7.3 log(10) copies/mL in urine. Among 56 non-BKVAN patients, 45 were negative for Q-BKDNA; 4 were positive in plasma with a median viral load of 4.8 log(10) copies/ mL, and 10 were positive in urine with a median viral load of 4.8 log(10) copies/mL. Receiver operating characteristic curve analysis showed that a cutoff of 4.5 log(10) copies/mL in plasma and a cutoff of 5.9 log(10) copies/mL in urine had a sensitivity of 100% and a specificity of 96.4%, respectively. A combined cutoffs of 4 log(10) copies/ mL in plasma and 6 log(10) copies/mL in urine had better performance with a sensitivity of 100% and a specificity of 98.2% than each cutoff of urine or plasma. QBKDNA with the combined cutoffs could reliably diagnose BKVAN in renal transplant recipients.
Adolescent
;
Adult
;
BK Virus/*genetics
;
Biopsy
;
Calibration
;
DNA, Viral/*genetics
;
Female
;
Humans
;
Kidney Diseases/*virology
;
Kidney Transplantation/*methods
;
Male
;
Middle Aged
;
Polymerase Chain Reaction
;
Polyomavirus Infections/diagnosis
;
Treatment Outcome
10.The Clinical Utility of Automated Breast Volume Scanner: A Pilot Study of 139 Cases.
Young Wook KIM ; Seon Kwang KIM ; Hyun Jo YOUN ; Eun Jung CHOI ; Sung Hoo JUNG
Journal of Breast Cancer 2013;16(3):329-334
PURPOSE: The aim of this study is to evaluate the clinical utility of automated breast volume scanner (ABVS) for detecting and diagnosing the breast lesions. METHODS: From December 2010 to January 2012, bilateral whole breast examinations were performed with ABVS for 139 women. Based on the Breast Imaging Reporting and Data System (BI-RADS) categories, the breast lesions were evaluated on coronal multiplanar reconstruction images using the ABVS workstation. Then, the imaging results were compared with those on conventional handheld ultrasound (HHUS) images. Histological diagnoses were performed on BI-RADS category 4 and 5 lesions. RESULTS: A total of 453 lesions were detected by ABVS. On the HHUS, 33 new lesions were detected but 69 lesions were not detected. BI-RADS category 2 and 3 matched to those on ABVS at 73.5% (61/83) and 85.4% (276/323). In 47 lesions of BI-RADS category 4 or 5, there was an exact match to those on ABVS. In addition, 47 lesions were classified as BI-RADS category 4 and 5, for which an ultrasound-guided core needle biopsy was performed. The malignant lesions of BI-RADS category 4 and 5 showed the following: 2/27 (7.4%) in 4A, 4/5 (80%) in 4B, 2/2 (100%) in 4C, and 13/13 (100%) in 5. The ABVS showed 21 true positives and a positive predictive value of 44.7% (21/47). CONCLUSION: There was considerable agreement in the assessment of the breast lesions by ABVS and HHUS. The ABVS had advantages of high diagnostic accuracy, examiner-independence, multislice visualization of the whole breast and less time-consuming. Our results indicate that ABVS might be a useful modality in diagnosing breast lesions.
Biopsy, Large-Core Needle
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Breast
;
Breast Neoplasms
;
Female
;
Humans
;
Information Systems
;
Pilot Projects