The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA ; Genetics, Population ; Humans ; Immunoglobulin Allotypes* ; Immunoglobulins* ; Lymphocytes ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

No abstract available.
Porokeratosis

No abstract available.
Humans* ; Intercellular Adhesion Molecule-1* ; Lung Neoplasms* ; Lung*

No abstract available.
Capillaries* ; Hemangioma, Capillary*

No abstract available.
Animals ; Horns* ; Keratoderma, Palmoplantar*

No abstract available.
Tattooing ; Warts

No abstract available.
Cystitis*

BACKGROUND: Direct antigen test (DAT) and culture are primary tests to diagnose infections by respiratory viruses, but are mainly available for the traditional viral pathogens such as respiratory syncytial virus (RSV), influenza virus, parainfluenza virus (PIV), and adenovirus in clinical laboratories. The objective of this study was to evaluate a multiplex reverse transcriptase-PCR method using Seeplex(TM) RV Detection kit (Seegene, Korea) for the detection of rhinovirus, coronavirus, and human metapneumovirus (hMPV). METHODS: From January to May 2007, nasopharyngeal aspirates (NPAs) from pediatric patients negative for culture and DAT of traditional viral pathogens were tested with Seeplex(TM). All the amplicons were directly sequenced and homology of the sequences was searched in the National Center for Biotechnology Information (NCBI) database. Patients' medical records were reviewed for clinical and demographic features. RESULTS: Forty-seven (26.4%) of 178 NPAs were positive: 18 rhinovirus, 15 hMPV, 4 RSV A, 3 coronavirus OC43, 3 influenza virus A, 2 adenovirus, 1 coronavirus NL63, and 1 RSV B. Based on maximum identity, each of the sequences indicating rhinovirus, hMPV, and coronavirus OC43 matched to the corresponding viruses with homology of 94-98%, 96-99%, and 98-100%, respectively. Seeplex(TM) positive patients were 0-11 yr old with a male:female ratio of 1.5:1. Clinical diagnoses included 9 pneumonia, 6 bronchiolitis, 2 cold, 1 asthma exacerbation for rhinovirus; 10 pneumonia, 4 bronchiolitis, and 1 clinical sepsis for hPMV; and 1 pneumonia, 2 croup, and 1 cold for coronavirus. CONCLUSIONS: Multiplex reverse transcriptase-PCR method using Seeplex(TM) RV Detection kit is a reliable test to detect rhinovirus, hMPV, and coronavirus. It may improve the diagnostic sensitivity for RSV, influenza virus, PIV, and adenovirus.
Adolescent ; Child ; Child, Preschool ; Coronavirus/classification/*isolation & purification ; Coronavirus 229E, Human/classification/genetics/isolation & purification ; Coronavirus OC43, Human/classification/genetics/isolation & purification ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Metapneumovirus/classification/genetics/*isolation & purification ; Phylogeny ; Reagent Kits, Diagnostic ; Respiratory Tract Infections/*diagnosis/virology ; Reverse Transcriptase Polymerase Chain Reaction/*methods ; Rhinovirus/classification/genetics/*isolation & purification ; Sequence Analysis, DNA

BACKGROUND/AIMS: Partial hepatectomy is considered as best treatment for selected HCC patients combined with liver cirrhosis. We conducted this study to assess the safety and effects of partial hepatectomy for HCC combined with liver cirrhosis and to identify the prognostic factors. METHODS: Retrospective analysis was performed for 115 HCC patients with liver cirrhosis who underwent hepatectomy in Korea Cancer Center Hospital from 1987 September to 2002 July. Median follow-up period was 30 months. RESULTS: In-hospital mortality rate was 0.87% (1/115 cases). 5-years overall and disease free survival rates were 59.3% and 44.75%, respectively. Fifty three patients developed recurrence and most common recurrent site was remnant liver (81.1%) and 3-year survival rate after recurrence was 33.4%. Independent prognostic factors for overall survival rate were TNM stage, multiplicity and venous invasion (p<0.05). CONCLUSION: Hepatectomy plays a significant role for HCC with compensated liver cirrhosis and is regarded as the effective treatment with acceptable mortality. However, recurrence rate is still high, so frequent follow-up study should be needed for early detection of recurrence amenable to effective treatment. For the high risk patients, further study should be followed to reduce the recurrence after hepatectomy, including surgical technique and adjuvant treatment.
Carcinoma, Hepatocellular* ; Disease-Free Survival ; Follow-Up Studies ; Hepatectomy* ; Hospital Mortality ; Humans ; Korea ; Liver Cirrhosis* ; Liver* ; Mortality ; Recurrence ; Retrospective Studies ; Survival Rate

PURPOSE: The aim of this study is to evaluate the clinical utility of automated breast volume scanner (ABVS) for detecting and diagnosing the breast lesions. METHODS: From December 2010 to January 2012, bilateral whole breast examinations were performed with ABVS for 139 women. Based on the Breast Imaging Reporting and Data System (BI-RADS) categories, the breast lesions were evaluated on coronal multiplanar reconstruction images using the ABVS workstation. Then, the imaging results were compared with those on conventional handheld ultrasound (HHUS) images. Histological diagnoses were performed on BI-RADS category 4 and 5 lesions. RESULTS: A total of 453 lesions were detected by ABVS. On the HHUS, 33 new lesions were detected but 69 lesions were not detected. BI-RADS category 2 and 3 matched to those on ABVS at 73.5% (61/83) and 85.4% (276/323). In 47 lesions of BI-RADS category 4 or 5, there was an exact match to those on ABVS. In addition, 47 lesions were classified as BI-RADS category 4 and 5, for which an ultrasound-guided core needle biopsy was performed. The malignant lesions of BI-RADS category 4 and 5 showed the following: 2/27 (7.4%) in 4A, 4/5 (80%) in 4B, 2/2 (100%) in 4C, and 13/13 (100%) in 5. The ABVS showed 21 true positives and a positive predictive value of 44.7% (21/47). CONCLUSION: There was considerable agreement in the assessment of the breast lesions by ABVS and HHUS. The ABVS had advantages of high diagnostic accuracy, examiner-independence, multislice visualization of the whole breast and less time-consuming. Our results indicate that ABVS might be a useful modality in diagnosing breast lesions.
Biopsy, Large-Core Needle ; Breast ; Breast Neoplasms ; Female ; Humans ; Information Systems ; Pilot Projects