The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
DNA ; Genetics, Population ; Humans ; Immunoglobulin Allotypes* ; Immunoglobulins* ; Lymphocytes ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

No abstract available.
Porokeratosis

No abstract available.
Humans* ; Intercellular Adhesion Molecule-1* ; Lung Neoplasms* ; Lung*

No abstract available.
Animals ; Horns* ; Keratoderma, Palmoplantar*

No abstract available.
Capillaries* ; Hemangioma, Capillary*

No abstract available.
Tattooing ; Warts

No abstract available.
Cystitis*

Quantitative measurement of BK virus DNA (Q-BKDNA) has been used for the early diagnosis and monitoring of BK virus-associated nephropathy (BKVAN). This study was designed to determine the BKDNA cutoff for the diagnosis of BKVAN. Between June 2005 and February 2007, 64 renal transplant recipients taken renal biopsies due to renal impairment submitted plasma and urine for Q-BKDNA. Eight BKVAN patients (12.5%) had median viral loads of 6.0 log(10) copies/mL in plasma and 7.3 log(10) copies/mL in urine. Among 56 non-BKVAN patients, 45 were negative for Q-BKDNA; 4 were positive in plasma with a median viral load of 4.8 log(10) copies/ mL, and 10 were positive in urine with a median viral load of 4.8 log(10) copies/mL. Receiver operating characteristic curve analysis showed that a cutoff of 4.5 log(10) copies/mL in plasma and a cutoff of 5.9 log(10) copies/mL in urine had a sensitivity of 100% and a specificity of 96.4%, respectively. A combined cutoffs of 4 log(10) copies/ mL in plasma and 6 log(10) copies/mL in urine had better performance with a sensitivity of 100% and a specificity of 98.2% than each cutoff of urine or plasma. QBKDNA with the combined cutoffs could reliably diagnose BKVAN in renal transplant recipients.
Adolescent ; Adult ; BK Virus/*genetics ; Biopsy ; Calibration ; DNA, Viral/*genetics ; Female ; Humans ; Kidney Diseases/*virology ; Kidney Transplantation/*methods ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polyomavirus Infections/diagnosis ; Treatment Outcome

PURPOSE: The aim of this study is to evaluate the clinical utility of automated breast volume scanner (ABVS) for detecting and diagnosing the breast lesions. METHODS: From December 2010 to January 2012, bilateral whole breast examinations were performed with ABVS for 139 women. Based on the Breast Imaging Reporting and Data System (BI-RADS) categories, the breast lesions were evaluated on coronal multiplanar reconstruction images using the ABVS workstation. Then, the imaging results were compared with those on conventional handheld ultrasound (HHUS) images. Histological diagnoses were performed on BI-RADS category 4 and 5 lesions. RESULTS: A total of 453 lesions were detected by ABVS. On the HHUS, 33 new lesions were detected but 69 lesions were not detected. BI-RADS category 2 and 3 matched to those on ABVS at 73.5% (61/83) and 85.4% (276/323). In 47 lesions of BI-RADS category 4 or 5, there was an exact match to those on ABVS. In addition, 47 lesions were classified as BI-RADS category 4 and 5, for which an ultrasound-guided core needle biopsy was performed. The malignant lesions of BI-RADS category 4 and 5 showed the following: 2/27 (7.4%) in 4A, 4/5 (80%) in 4B, 2/2 (100%) in 4C, and 13/13 (100%) in 5. The ABVS showed 21 true positives and a positive predictive value of 44.7% (21/47). CONCLUSION: There was considerable agreement in the assessment of the breast lesions by ABVS and HHUS. The ABVS had advantages of high diagnostic accuracy, examiner-independence, multislice visualization of the whole breast and less time-consuming. Our results indicate that ABVS might be a useful modality in diagnosing breast lesions.
Biopsy, Large-Core Needle ; Breast ; Breast Neoplasms ; Female ; Humans ; Information Systems ; Pilot Projects

Acute optic neuropathy occurs commonly as a result of ischemia and inflammation, but paranasal sinus disease without infection is generally dismissed. We report a rare case of acute optic neuropathy caused by compression of a mucocele in an Onodi cell, which is an uncommon anatomical variation of the ethmoid sinus. The mucocele was confirmed by imaging and endoscopic sinus surgery. An Onodi-cell lesion should be considered in the differential diagnosis of acute optic neuropathy.
Diagnosis, Differential ; Ethmoid Sinus ; Inflammation ; Ischemia ; Mucocele ; Optic Nerve Diseases ; Paranasal Sinus Diseases