1.Korea Seroprevalence Study of Monitoring of SARS-COV-2 Antibody Retention and Transmission (K-SEROSMART): findings from national representative sample
Jina HAN ; Hye Jin BAEK ; Eunbi NOH ; Kyuhyun YOON ; Jung Ae KIM ; Sukhyun RYU ; Kay O LEE ; No Yai PARK ; Eunok JUNG ; Sangil KIM ; Hyukmin LEE ; Yoo-Sung HWANG ; Jaehun JUNG ; Hun Jae LEE ; Sung-il CHO ; Sangcheol OH ; Migyeong KIM ; Chang-Mo OH ; Byengchul YU ; Young-Seoub HONG ; Keonyeop KIM ; Sunjae JUNG ; Mi Ah HAN ; Moo-Sik LEE ; Jung-Jeung LEE ; Young HWANGBO ; Hyeon Woo YIM ; Yu-Mi KIM ; Joongyub LEE ; Weon-Young LEE ; Jae-Hyun PARK ; Sungsoo OH ; Heui Sug JO ; Hyeongsu KIM ; Gilwon KANG ; Hae-Sung NAM ; Ju-Hyung LEE ; Gyung-Jae OH ; Min-Ho SHIN ; Soyeon RYU ; Tae-Yoon HWANG ; Soon-Woo PARK ; Sang Kyu KIM ; Roma SEOL ; Ki-Soo PARK ; Su Young KIM ; Jun-wook KWON ; Sung Soon KIM ; Byoungguk KIM ; June-Woo LEE ; Eun Young JANG ; Ah-Ra KIM ; Jeonghyun NAM ; ; Soon Young LEE ; Dong-Hyun KIM
Epidemiology and Health 2023;45(1):e2023075-
OBJECTIVES:
We estimated the population prevalence of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including unreported infections, through a Korea Seroprevalence Study of Monitoring of SARS-CoV-2 Antibody Retention and Transmission (K-SEROSMART) in 258 communities throughout Korea.
METHODS:
In August 2022, a survey was conducted among 10,000 household members aged 5 years and older, in households selected through two stage probability random sampling. During face-to-face household interviews, participants self-reported their health status, COVID-19 diagnosis and vaccination history, and general characteristics. Subsequently, participants visited a community health center or medical clinic for blood sampling. Blood samples were analyzed for the presence of antibodies to spike proteins (anti-S) and antibodies to nucleocapsid proteins (anti-N) SARS-CoV-2 proteins using an electrochemiluminescence immunoassay. To estimate the population prevalence, the PROC SURVEYMEANS statistical procedure was employed, with weighting to reflect demographic data from July 2022.
RESULTS:
In total, 9,945 individuals from 5,041 households were surveyed across 258 communities, representing all basic local governments in Korea. The overall population-adjusted prevalence rates of anti-S and anti-N were 97.6% and 57.1%, respectively. Since the Korea Disease Control and Prevention Agency has reported a cumulative incidence of confirmed cases of 37.8% through July 31, 2022, the proportion of unreported infections among all COVID-19 infection was suggested to be 33.9%.
CONCLUSIONS
The K-SEROSMART represents the first nationwide, community-based seroepidemiologic survey of COVID-19, confirming that most individuals possess antibodies to SARS-CoV-2 and that a significant number of unreported cases existed. Furthermore, this study lays the foundation for a surveillance system to continuously monitor transmission at the community level and the response to COVID-19.
2.Developing a Nursing Needs Assessment Scale and Patient Classification System based on Nursing Activities in Comprehensive Nursing Care Units
Jeonghyun KIM ; Sujin SHIN ; Sung-Heui BAE ; Inyoung LEE
Journal of Korean Academy of Fundamental Nursing 2021;28(3):395-409
Purpose:
This study was done to develop and validate a scale for assessing nursing needs on comprehensive nursing care units and to derive a patient classification system based on nursing needs.
Methods:
In this methodological study, the initial items were identified through a review of the literature and surveys from nursing staff regarding the nursing needs on comprehensive nursing care units. Content validity was evaluated by nine nursing staff members from comprehensive nursing care units. To evaluate the concurrent validity and derive a patient classification system, nursing needs scores, perceived nursing needs and perceived patient severity for 216 patient cases were evaluated by five nurses. These data were analyzed using Pearson‘s correlation coefficients, one-way ANOVA with Scheffépost hoc tests and K-means clustering.
Results:
After evaluating content validity, the developed scale contained 64 activities in two domains: nursing intervention and assistance of daily living. Concurrent validity was verified by analyzing the differences in the nursing needs scores according to each group of perceived nursing needs and severity (p<.001) and by analyzing the correlation between the score of the developed scale and the National Health Insurance Service nursing need assessment scale (r=.68, p<.001). Based on the score of the developed scale, a patient classification system that classified nursing needs into four stages was derived.
Conclusion
The developed scale represented nursing activities in comprehensive nursing care units. It also provided specific data regarding the time spent on nursing activities. Therefore, it is expected to contribute toward establishing appropriate nurse staffing strategies to provide quality patient care.
3.Clinical Practice Guideline for Cardiac Rehabilitation in Korea
Chul KIM ; Jidong SUNG ; Jong Hwa LEE ; Won Seok KIM ; Goo Joo LEE ; Sungju JEE ; Il Young JUNG ; Ueon Woo RAH ; Byung Ok KIM ; Kyoung Hyo CHOI ; Bum Sun KWON ; Seung Don YOO ; Heui Je BANG ; Hyung Ik SHIN ; Yong Wook KIM ; Heeyoune JUNG ; Eung Ju KIM ; Jung Hwan LEE ; In Hyun JUNG ; Jae Seung JUNG ; Jong Young LEE ; Jae Young HAN ; Eun Young HAN ; Yu Hui WON ; Woosik HAN ; Sora BAEK ; Kyung Lim JOA ; Sook Joung LEE ; Ae Ryoung KIM ; So Young LEE ; Jihee KIM ; Hee Eun CHOI ; Byeong Ju LEE ; Soon KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 2019;52(4):248-285
BACKGROUND: Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. METHODS: This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of 33 authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. RESULTS: CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers cardiovascular mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. CONCLUSION: Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.
Acute Coronary Syndrome
;
Cardiovascular Diseases
;
Cause of Death
;
Consultants
;
Coronary Disease
;
Delivery of Health Care
;
Heart
;
Hospitals, General
;
Hospitals, University
;
Humans
;
Insurance Benefits
;
Korea
;
Life Style
;
Methods
;
Mortality
;
Myocardial Infarction
;
Nutritionists
;
Physical Therapists
;
Preventive Medicine
;
Recurrence
;
Rehabilitation
;
Secondary Prevention
;
Specialization
;
Sports Medicine
;
Surgeons
4.Clinical Practice Guideline for Cardiac Rehabilitation in Korea Online only
Chul KIM ; Jidong SUNG ; Jong Hwa LEE ; Won Seok KIM ; Goo Joo LEE ; Sungju JEE ; Il Young JUNG ; Ueon Woo RAH ; Byung Ok KIM ; Kyoung Hyo CHOI ; Bum Sun KWON ; Seung Don YOO ; Heui Je BANG ; Hyung Ik SHIN ; Yong Wook KIM ; Heeyoune JUNG ; Eung Ju KIM ; Jung Hwan LEE ; In Hyun JUNG ; Jae Seung JUNG ; Jong Young LEE ; Jae Young HAN ; Eun Young HAN ; Yu Hui WON ; Woosik HAN ; Sora BAEK ; Kyung Lim JOA ; Sook Joung LEE ; Ae Ryoung KIM ; So Young LEE ; Jihee KIM ; Hee Eun CHOI ; Byeong Ju LEE ; Soon KIM
Annals of Rehabilitation Medicine 2019;43(3):355-356
OBJECTIVE: Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and three additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. METHODS: This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of 33 authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and two general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, three rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. PRINCIPAL CONCLUSIONS: CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers cardiovascular mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.
Acute Coronary Syndrome
;
Cardiovascular Diseases
;
Cause of Death
;
Consultants
;
Coronary Disease
;
Delivery of Health Care
;
Heart
;
Hospitals, General
;
Hospitals, University
;
Humans
;
Insurance Benefits
;
Korea
;
Life Style
;
Methods
;
Mortality
;
Myocardial Infarction
;
Nutritionists
;
Physical Therapists
;
Preventive Medicine
;
Recurrence
;
Rehabilitation
;
Secondary Prevention
;
Specialization
;
Sports Medicine
;
Surgeons
5.The Relationship between Parental Stress and Nurses' Communication as Perceived by Parents of High-risk Newborns
Chang Hee LEE ; Mi Heui JANG ; Yong Sung CHOI ; Hyunsook SHIN
Child Health Nursing Research 2019;25(2):184-195
PURPOSE: This study aimed to characterize the relationship between parental stress and nurses' communication as perceived by parents of high-risk newborns in a neonatal intensive care unit (NICU). METHODS: The participants were 54 parents of high-risk newborns in a NICU. Data were collected from January to March 2018. Parental stress and parents' perceptions of nurses' communication ability and styles were measured using a questionnaire. RESULTS: The average scores for parental stress and nurses' communication ability were 3.39 and 4.38 respectively, on a 5-point scale. Parents most commonly reported that nurses showed a friendly communication style, followed by informative and authoritative styles. Mothers and fathers reported significantly different levels of parental stress. Parental stress showed a negative correlation with nurses' perceived verbal communication ability. Higher scores for nurses' verbal communication ability and for friendly and informative communication styles were associated with lower parental stress induced by the environment, the baby's appearance and behaviors, and treatments in the NICU. CONCLUSION: The findings of this study suggest that nurses need to offer proper information for parents and to support parents by encouraging them to express their emotions of stress and by providing parents with therapeutic communication and opportunities to participate in care.
Communication
;
Fathers
;
Humans
;
Infant, Newborn
;
Intensive Care Units, Neonatal
;
Intensive Care, Neonatal
;
Mothers
;
Parents
;
Stress, Psychological
6.Clinical Practice Guideline for Cardiac Rehabilitation in Korea: Recommendations for Cardiac Rehabilitation and Secondary Prevention after Acute Coronary Syndrome
Chul KIM ; Jidong SUNG ; Jong Hwa LEE ; Won Seok KIM ; Goo Joo LEE ; Sungju JEE ; Il Young JUNG ; Ueon Woo RAH ; Byung Ok KIM ; Kyoung Hyo CHOI ; Bum Sun KWON ; Seung Don YOO ; Heui Je BANG ; Hyung Ik SHIN ; Yong Wook KIM ; Heeyoune JUNG ; Eung Ju KIM ; Jung Hwan LEE ; In Hyun JUNG ; Jae Seung JUNG ; Jong Young LEE ; Jae Young HAN ; Eun Young HAN ; Yu Hui WON ; Woosik HAN ; Sora BAEK ; Kyung Lim JOA ; Sook Joung LEE ; Ae Ryoung KIM ; So Young LEE ; Jihee KIM ; Hee Eun CHOI ; Byeong Ju LEE ; Soon KIM
Korean Circulation Journal 2019;49(11):1066-1111
Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular (CV) disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of thirty-three authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers CV mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.
Acute Coronary Syndrome
;
Cause of Death
;
Consultants
;
Coronary Disease
;
Delivery of Health Care
;
Heart
;
Hospitals, General
;
Hospitals, University
;
Humans
;
Insurance Benefits
;
Korea
;
Life Style
;
Methods
;
Mortality
;
Myocardial Infarction
;
Nutritionists
;
Physical Therapists
;
Preventive Medicine
;
Recurrence
;
Rehabilitation
;
Secondary Prevention
;
Specialization
;
Sports Medicine
;
Surgeons
7.Clinical Practice Guideline for Cardiac Rehabilitation in Korea
Chul KIM ; Jidong SUNG ; Jong Hwa LEE ; Won Seok KIM ; Goo Joo LEE ; Sungju JEE ; Il Young JUNG ; Ueon Woo RAH ; Byung Ok KIM ; Kyoung Hyo CHOI ; Bum Sun KWON ; Seung Don YOO ; Heui Je BANG ; Hyung Ik SHIN ; Yong Wook KIM ; Heeyoune JUNG ; Eung Ju KIM ; Jung Hwan LEE ; In Hyun JUNG ; Jae Seung JUNG ; Jong Young LEE ; Jae Young HAN ; Eun Young HAN ; Yu Hui WON ; Woosik HAN ; Sora BAEK ; Kyung Lim JOA ; Sook Joung LEE ; Ae Ryoung KIM ; So Young LEE ; Jihee KIM ; Hee Eun CHOI ; Byeong Ju LEE ; Soon KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 2019;52(4):248-285
BACKGROUND:
Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea.
METHODS:
This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of 33 authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified.
RESULTS:
CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers cardiovascular mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR.
CONCLUSION
Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.
8.Clinical Practice Guideline for Cardiac Rehabilitation in Korea: Recommendations for Cardiac Rehabilitation and Secondary Prevention after Acute Coronary Syndrome
Chul KIM ; Jidong SUNG ; Jong Hwa LEE ; Won Seok KIM ; Goo Joo LEE ; Sungju JEE ; Il Young JUNG ; Ueon Woo RAH ; Byung Ok KIM ; Kyoung Hyo CHOI ; Bum Sun KWON ; Seung Don YOO ; Heui Je BANG ; Hyung Ik SHIN ; Yong Wook KIM ; Heeyoune JUNG ; Eung Ju KIM ; Jung Hwan LEE ; In Hyun JUNG ; Jae Seung JUNG ; Jong Young LEE ; Jae Young HAN ; Eun Young HAN ; Yu Hui WON ; Woosik HAN ; Sora BAEK ; Kyung Lim JOA ; Sook Joung LEE ; Ae Ryoung KIM ; So Young LEE ; Jihee KIM ; Hee Eun CHOI ; Byeong Ju LEE ; Soon KIM
Korean Circulation Journal 2019;49(11):1066-1111
Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular (CV) disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of thirty-three authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers CV mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.
9.Work-related Characteristics and Sleep Quality of Nurses in Comprehensive Nursing Care Units of Small-medium Sized Hospitals
Sujin SHIN ; Inyoung LEE ; Jeonghyun KIM ; Sung Heui BAE
Journal of Korean Academy of Fundamental Nursing 2019;26(4):260-268
PURPOSE:
The purpose of this study was to examine the sleep quality of nurses in the comprehensive nursing care units of small-medium sized hospitals, and general and work-related characteristics associated with sleep quality of nurses.
METHODS:
Participants were 312 nurses in comprehensive nursing care units of eight small and medium hospitals. The data were collected between June and August, 2018. A survey questionnaire including, sleep quality, general, and work-related characteristics of nurses was used. The data were analyzed using SPSS/WIN 23.0 program with descriptive statistics, independent t-test, and χ² test. Logistic regression analyses were used to identify factors associated with sleep quality.
RESULTS:
The average score for sleep quality was 8.58±3.18 (0~21). Using a cut-off point of 8.5, 51.9% were poor sleepers. Sleep quality was associated with age (Odds Ratio [OR]=0.93, 95% Confidence Interval [CI]=0.88~0.99).
CONCLUSION
Age was identified as a factor associated with sleep quality among nurses in the comprehensive nursing care units at small and medium sized hospitals. Researchers and nurse administrators need to develop and provide strategies to improve young nurses' sleep quality.
10.Comparison of the Utility of dnaJ and 16S rDNA Sequences for Identification of Clinical Isolates of Vibrio Species
In Sun CHOI ; Dae Soo MOON ; Geon PARK ; Seong Ho KANG ; Choon Mee KIM ; Young Joon AHN ; Dong Min KIM ; Na Ra YUN ; Dong Hoon LIM ; Sung Heui SHIN ; Joong Ki KOOK ; Young Hyo CHANG ; Sook Jin JANG
Laboratory Medicine Online 2018;8(1):7-14
BACKGROUND: Among the many Vibrio species that can cause infections in humans, several species can cause a fatal outcome. Therefore, accurate identification of Vibrio species is very important. Since some species show atypical phenotypic features, selecting an appropriate molecular method is necessary to avoid misdiagnosis. METHODS: Vibrio clinical isolates (N=53) and reference strains (N=8) were used in this study. We analyzed the following sequences for identification: dnaJ gene, 16S rDNA, gyrase B (gyrB) V. vulnificus-specific sequence, gyrB V. navarrensis-specific sequence, and V. vulnificus hemolysin gene PCR (Vvh PCR). We performed phylogenetic analysis of the 16S rDNA, dnaJ, and gyrB sequences. Final identification was based on the combined results of all tests described above. Concordance of the 16S rDNA and dnaJ sequence analysis was measured using the Chi-square test. RESULTS: The 61 Vibrio strains were identified as follows, in descending order: V. vulnificus (78.69%), V. parahaemolyticus (6.56%), V. navarrensis (4.92%), V. mimicus (1.64%), V. cholera (1.64%), V. furnissii (1.64%), V. alginolyticus (1.64%), and Grimontia hollisae (1.64%). The accuracy rates of the dnaJ gene and 16S rDNA sequence for identification were 91.80% and 86.89%, respectively. The 16S rDNA and dnaJ sequences showed a concordance rate of 0.45, which indicates moderate agreement. CONCLUSIONS: Our results suggest that analysis of the dnaJ sequence may be a useful method for the identification of clinical isolates of Vibrio species, especially for distinguishing between closely related Vibrio species.
Cholera
;
Diagnostic Errors
;
DNA, Ribosomal
;
Fatal Outcome
;
Humans
;
Methods
;
Polymerase Chain Reaction
;
Sequence Analysis
;
Vibrio

Result Analysis
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