No abstract available.
Dentists ; Humans

The form of furcation influence both the pathogenesis of periodontal destruction and therapeutic results. The present study was performed to evaluate the effect of root trunk length on clinical outcomes of guided tissue regeneration. Total 30 mandibular first molars were evaluated in this study. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were measured at baseline and 6 month after GTR. Correlation coefficients between root trunk length and other clinical measurement were analyzed. The results of this study were as follows 1. The mean root trunk length in lower 1st molar was 2.15 mm. 2. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were significantly reduced at 6 month postoperatively compared to values of baseline 3. Correlation coefficient between root trunk length and vertical defect depth at baseline was 0.406 showing the positive correlation 4. Correlation coefficient between root trunk length and horizontal defect depth at baseline was -0.463 showing the negative correlation. 5. Correlation coefficient between root trunk length and decrease of horizontal defect depth after GTR was 0.654 showing the positive correlation. In conclusion, the root trunk length maybe effector for clinical outcome after guided tissue regeneration.

DFDBA(Decalcified freeze-dried bone allograft) is one of the allograft materials for periodontal bone regeneration. DFDBA provides an osteoconductive surface and osteoinductive factors. Therefore, DFDBA have been used successfully to regenerate the attachment apparatus during periodontal treatment. But recent studies was reported that wide variations in commercial bone bank preparations of DFDBA do exist, including the ability to induce new bone formation. DFDBA was experimental materials that was recovered, processed, tested, shipped and invoiced through Musculoskeletal Transplant Foundation. MTF(Musculoskeletal Transplant Foundation) is the world largest, non-profit, AATB(American Association of Tissue Banks) accredited tissue bank. The objective of this study was to determine the effects of serial dilutions of a DFDBA on human fetal osteoblastic cell proliferation and their potential to form and mineralize bone nodules. Human fetal osteoblastic cell line(hFOB 1.19) was cultured with DMEM and SSE(1microgram/ml, 10microgram/ml, 100 microgram/ml, 1mg/ml) at 34degrees C with 5% CO2 in 100% humidity. Cell proliferation was significantly increased at 1mg/ml, 100microgram/ml, 10microgram/ml, 1microgram/ml, 100ng/ml, 10ng/ml, 1ng/ml of DFDBA after 5 days incubation (p<0.05). Alkaline Phosphatase(ALP) level was significantly increased in 100ng/ml, 10ng/ml, 1ng/ml of DFDABA(p<0.05). A quantified calcium accumulation was significantly increased at 1ng/ml, 10ng/ml of MTF(p<0.05). These results indicated that DFDBA has an inductive effect on bone formation in vitro.
Humans

The purpose of this study was to investigate the effects of ICB(Irradiated frozen allogenic bone, Rocky Mountain Tissue Bank, USA) and MTF(Decalcified freeze-dried bone allograft, Musculoskeletal Transplant Foundation, USA) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts (hFOB1) were cultured with 10 ng/ml of ICB and MTF. The negatvie control group was cultured with DMSO and positive control group was cultured with BMP (2 ng/ml). MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Calcium accumulation was also evaluated. ICB and MTF did not increase the rate of the cellular proliferation of hFOB1s while they enhanced ALP and calcium accumulation. The expression of osteocalcin (OC) and bone silaloprotein (BSP) increased in hFOB1 treated with ICB and MTF (10 ng/ml). These results suggest that ICB and MTF stimulate osteoblastic activity of the hFOB1.
Humans

PURPOSE: The concept of gingival biotype has been used as a predictor of periodontal therapy outcomes since the 1980s. In the present study, prospective and controlled experiments were performed to compare periodontal pocket depth (PPD) reduction and gingival shrinkage (GSH) after scaling and root planing (SRP) according to gingival biotype. METHODS: Twenty-five patients diagnosed with chronic periodontitis participated in the present study. The PPD and GSH of the labial side of the maxillary anterior teeth (from the right canine to the left canine) were evaluated at baseline and 3 months after SRP. Changes in the PPD following SRP were classified into 4 groups according to the gingival thickness and initial PPD. Two more groups representing normal gingival crevices were added in evaluation of the GSH. The results were statistically analyzed using the independent t-test. RESULTS: In the end, 16 patients participated in the present study. With regard to PPD reduction, there were no significant differences according to gingival biotype (P>0.05). Likewise, sites with a PPD of over 3 mm failed to show any significant differences in the GSH (P>0.05). However, among the sites with a PPD of under 3 mm, those with the thin gingival biotype showed more GSH (P<0.05). CONCLUSIONS: PPD changes after SRP were not affected by gingival biotype with either shallow or deep periodontal pockets. GSH also showed equal outcomes in all the groups without normal gingival crevices. The results of SRP seem not to differ according to gingival biotype.
Chronic Periodontitis ; Dental Scaling ; Humans ; Periodontal Pocket ; Prospective Studies ; Root Planing* ; Tooth

This study was designed to compare the effects of treatment using chitosan membrane (Nanogide-C(R)) resorbable barrier with control treated by polylactic acid/polylacticglycolic acid membrane(PLA/PLGA membrane, Biomesh(R)). 44 furcation defecs from 44 patients with class 2 furcation degree were used for this study, 22 sites of them were treated by chitosan membrane as experimental group and 22 site were treated by PLA/PLGA membrane as control group. Clinical parameters including probing depth, gingival recession, attachment level and radiographic examination were evlauated at base line, 1 month, 2 month and 3 month. after surgery. Statistical test used to analyze these data included paired t-test, one way ANOVA. The results are as follows : 1. Probing depth was significanlly decreased in the two group and there were significant differences between groups(p<0.05). 2. Gingival recession was not significanlly increased in the two group and there were no significant differences between groups(p<0.05). 3. Loss of attachment was statistically decreased in the two group and there were no significant differences between groups(p<0.05). 4. Horizontal bone level was significanlly increased in the two group and there were significant differences between groups(p<0.05). On the basis of these results, chitoans resorbable membrane has similar potential to PLA/PLGA membrane in GTR for furcation defect.
Chitosan ; Furcation Defects* ; Gingival Recession ; Guided Tissue Regeneration* ; Humans ; Membranes

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.
Adult Stem Cells* ; Adult* ; Alkaline Phosphatase ; Blotting, Western ; Bone Marrow ; Calcium* ; Cell Count ; Cell Proliferation ; Collagen ; Humans ; Integrin-Binding Sialoprotein ; Mesenchymal Stromal Cells ; Osteoblasts* ; Osteocalcin ; Stem Cells

Gingival fibroblasts are embedded in an extracellular matrix. The matrixs have influence on the development, polarity, and behavior of nearby cells. The major component of periodontal extracellular matrix is a glycosaminoglycan. The glycosaminoglycan are large carbohydrates that are composed of repeating disaccharide units and exist in three main form: dermatan sulfate, chondrotitin sulfate, heparan sulfate. The purpose of present study is to examine the biologic effects of glycosaminoglycan on human gingival fibroblast. Human gingival fibroblasts were supplemented with each glycosaminoglycan, and cellular attachment and proliferation was determined by MTT assay. Dermatan sulfate and chondroitin sulfate did not stimulate the attachment and proliferation of human gingival fibroblasts, but heparan sulfate increased the proliferation and attachment in a time- and dose- dependent manner. These results indicated that heparan sulfate seems to have a high potential for gingival regeneration and root surface attachment.
Carbohydrates ; Chondroitin Sulfates ; Dermatan Sulfate ; Extracellular Matrix ; Fibroblasts* ; Heparitin Sulfate ; Humans* ; Regeneration

Many natural medicines have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential in periodontal tissues. Safflower seed has been traditionally used as a drug for treatment of bone fracture in oriental medicine. The purpose of the present study was to compare the effects of safflower seed extract and bone substitute on bone formation and regeneration in artificial defects in mongrel dogs. The bony defects were made with round bur at mandible and tibia. Extracts of safflower seed and bovine bone were placed directly at each defect for experimental group, and the defect of control group was sutured without any other treatment. Experimental animals were sacrificed at 8 weeks. And then histopathologic reading and histomorphometric study was done. There was not significant differences between control and experimental groups in osteoclastic activity and infiltration of inflammatory cells. However, new capillary proliferation, fibrosis and new bone formation were prominent in safflower seed extract group. The mandibular defects of safflower seed extract group were healed with dense connective and bony tissues, and endochondral bone formation was observed in tibial defect of safflower seed extract group only. New bone area of safflower seed extract group was more significantly increased than that of control and that of bone substitute group. These results indicate that direct local application of safflower seed extracts on bony defects seems to reduces the early inflammatory response and to promotes the bone regeneration.
Animals ; Bone Regeneration ; Bone Substitutes ; Capillaries ; Carthamus tinctorius* ; Dogs* ; Fibrosis ; Fractures, Bone ; Mandible ; Medicine, East Asian Traditional ; Osteoclasts ; Osteogenesis ; Regeneration* ; Tibia

PURPOSE: This study was designed to evaluated the relationship between the morphological changes of hypoxic-ischemic injured brain and the magnetic resonance spectroscopic (MRS) findings, and the efficacy of Egb 761, a free radical scavenger, as a neuroprotective agent for hypoxic-ischemic brain injury. METHODS: Seven-day old Sprague-Dawley rat pups were used. Right common carotid artery was ligated under halothene anesthesia. After a recovery period of 3 hours, they were divided into two groups : for group 1, EGb 761 100 mg/kg (treatment group) and for group 2, normal saline (control group) were given intraperitoneally. Both groups were exposed to 8% oxygen at 37degrees C for 90 minutes. Sixty-four rat pups (30 control, 34 treatment group) were examined with localized 1H MRS on days 1 and 7 after the hypoxic insult. One day 14, the degree of brain injury was scored by morphological changes. RESULTS: The 1H MRS obtained on day 1 after the hypoxic insult showed increased Lip/NAA and Lip/Cr ratios in the right cerebral hemisphere in comparison with those in the left hemisphere (P<0.01). The degree of morphological changes of the brain injury on day 14 correlated with both Lip/NAA and Lip/Cr ratios obtained on days! and 7 after the hypoxic insult [r=0.410, 0.457 on day 1, and r=0.749, 0.720 on day 7, respectively (P< 0.01)]. The therapeutic potential of EGb 761 for the hypoxic-ischemic brain seemed insignificant, as was evident from no differences between the control group and the EGb 761 treatment group in the Lip/NAA and Lip/Cr ratios as well as in morphological changes. CONCLUSION: Early changes of Lip/NAA and Lip/Cr ratios in the 1H MRS seems to be related to the degree of morphological changes in the rat brain of hypoxic-ischemic injury. Therefore, the increased lipid content of brain observed by 1H MRS can be used as a marker to predict the degree of the hypoxic-ischemic injury. The neuroprotectiveeffect of EGb 761 in hypoxic-ischemic brain injury is insignificant.
Anesthesia ; Animals ; Brain Injuries ; Brain* ; Carotid Artery, Common ; Cerebrum ; Magnetic Resonance Spectroscopy* ; Neuroprotective Agents* ; Oxygen ; Protons* ; Rats* ; Rats, Sprague-Dawley