1.Prevalence of antibody to cytomegalovirus by anticomplement immunofluorescence test in Korean children.
Hwan Jong LEE ; Sung Hee CHANG
Korean Journal of Infectious Diseases 1993;25(4):357-361
No abstract available.
Child*
;
Cytomegalovirus*
;
Fluorescent Antibody Technique*
;
Humans
;
Prevalence*
2.A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida) .
Sung Hee PARK ; Cheon Young CHANG ; Sung Shik SHIN
The Korean Journal of Parasitology 2005;43(4):141-147
A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of 22 x 40 mm in size was mounted at a height of 0.8 mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of copepods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to copepods during the handling process.
Protozoa
;
Male
;
Female
;
Culture Techniques/*methods
;
Culture Media
;
Copepoda/*growth & development
;
Animals
3.Esophageal Actinomycosis after Insertion of Esophageal Stent: A Case of Surgical Experience.
Sung Rae CHO ; Hyun Woo SHIN ; Hee Kyung CHANG
The Korean Journal of Thoracic and Cardiovascular Surgery 2000;33(7):601-604
Actinomycosis is an indolent, suppurative infection caused by an anaerobic gram-positive organism (usually actinomyces israelii) which usually causes infection in the face, mediastitum, lung, and abdomen. Primary esophageal actinomycosis which is not related with pulmonary or mediastinal actinomycosis, is very rare, especially in immunocompetent host. A 58-year-old woman has been suffered from dysphagia, odynophagia, and chest pain after insertion of esophageal stent in esophageal acid stricture. She underwent a esophagectomy with esophagogastrostomy for above mentioned symptoms. Pathologic diagnosis was a esophageal actinomycosis.
Abdomen
;
Actinomyces
;
Actinomycosis*
;
Chest Pain
;
Constriction, Pathologic
;
Deglutition Disorders
;
Diagnosis
;
Esophagectomy
;
Female
;
Humans
;
Lung
;
Middle Aged
;
Stents*
4.Spect assessment of regional cerebral perfusion abnormallity in head injury.
Kyung Han LEE ; Chul Hee KIM ; Ha Sung CHANG
Korean Journal of Nuclear Medicine 1992;26(2):235-243
No abstract available.
Craniocerebral Trauma*
;
Head*
;
Perfusion*
;
Tomography, Emission-Computed, Single-Photon*
5.Brain asymmetry in visual recognition of Hangul and Chinese words.
Hee Sang LEE ; Sung Kil MIN ; Chang Soon YANG
Journal of Korean Neuropsychiatric Association 1992;31(6):1100-1107
No abstract available.
Asian Continental Ancestry Group*
;
Brain*
;
Humans
6.Brain asymmetry in visual recognition of Hangul and Chinese words.
Hee Sang LEE ; Sung Kil MIN ; Chang Soon YANG
Journal of Korean Neuropsychiatric Association 1992;31(6):1100-1107
No abstract available.
Asian Continental Ancestry Group*
;
Brain*
;
Humans
7.How do the schizophrenic patients perceive others expressed emotion?.
Sung Gon KIM ; Chang Hee HONG ; Myung Jung KIM
Journal of Korean Neuropsychiatric Association 1992;31(3):545-555
No abstract available.
Expressed Emotion*
;
Humans
8.The Significance of the Expression of p53, E-cadherin, nm23, CD44, and Tumor Angiogenesis in Colorectal Adenocarcinoma.
Sung Suk PAENG ; Hee Jin CHANG ; Jung Il SUH
Korean Journal of Pathology 1997;31(4):314-325
Many oncogenes and tumor supressor genes have been identified and studied in colorectal carcinoma. Among them, p53 is a tumor supressor gene and its mutation is frequently noted in human tumors. E-cadherin is a cell adhesion molecule and associated with tumor differentiation. CD44 is a cell surface glycoprotein that plays a role in cell migration and metastasis. nm23 is a gene known to lower metastatic potential of tumors and has been proposed to be a metastasis supressor gene. Tumor angiogenesis is required for the expansion of the primary tumor and metastasis and its degree is related to the potential of malignancy. We studied the expression of p53, E-cadherin, nm23, CD44 and tumor angiogenesis in 36 cases of colorectal adenocarcinomas. They were compared with previously known prognostic factors such as the stage, tumor size, depth of invasion, differentiation, presence of lymphatic or venous invasion, the lymph node and distant metastasis. The results were as follows. 1) The expression of p53 was not significantly associated with any prognostic factors. 2) The expression of E-cadherin was significantly associated with tumor differentiation. In the well differentiated adenocarcinomas, its expression was higher than in the poorly differentiated adenocarcinoma. 3) The expression of nm23 was also significantly associated with tumor differentiation. In carcinoma with lymph node metastasis, the expression of nm23 was reduced, but statistically it was not significant. 4) The expression of CD44 was higher in tumors with lymph node metastasis than in tumors without lymph node metastasis, but it was not statistically significant. 5) The degree of microvessel density was significantly associated with lymphatic invasion. According to the above results, the expression of E-cadherin and nm23 are related to the differentiation of the tumor and tumor angiogenesis is related to the lymphatic invasion of the colorectal adenocarcinoma.
Adenocarcinoma*
;
Cadherins*
;
Cell Adhesion
;
Cell Movement
;
Colorectal Neoplasms
;
Genes, vif
;
Humans
;
Lymph Nodes
;
Membrane Glycoproteins
;
Microvessels
;
Neoplasm Metastasis
;
Oncogenes
;
von Willebrand Factor
9.Mutation of gyrA in Quinolone-Resistant Clinical Isolates of Escherichia coli.
Sung Yong SEOL ; Yoo Chul LEE ; Hee Kyung CHANG
Journal of the Korean Society for Microbiology 1999;34(2):103-113
To study the gyrA mutations of E. coli from clinical specimens, 410 strains were isolated from 1994 to 1997 in Kyungpook National Vniversity hospital. Antimicrobial susceptibility tests, PCR and sequencing of gyrA, and in vitro induction of quinolone resistance were done. The frequency of quinolone resistant E. coli strains increased constantly during 1994 through 1996. Quinolone-resistant strains were more often resistant to unrelated antibiotics than quinolone-susceptible strains (chi-square test, p<0.05). All of the randomly selected 55 quinolone- resist#ant strains were highly resistant to nalidixic acid (NAL) but had low level resistance to fluoroquinolones. All of the 55 quinolone-resistant strains showed an amino acid substitution of Ser -> Leu (TCG -> TIG) at codon 83. In addition, four different types of amino acid substitution affecting codon 87 (Asp) were detected, 1) type I: Asn (GAC -> AAC); 2) type II: Tyr (GAC -> TAC); 3) type III: Oly (GAC -> GGC); 4) type IV: His (GAC -> CAC). The mutation of type IV has not been reported previously in quinolone-resistant E. coli strains. It is thought that the specific amino acid substitution probably affects minimum inhibitory concentrations (MIC) of quinolones because the MICs of ciprofloxacin, norfloxacin, and ofloxacin in type II were significantly higher than those of type I. By in vitro induction, MICs to quinolone-susceptible strains resulted in the increase in the MICs of all quinolones tested by 2- to 2048-fold. The induced mutants by quinolones had amino acid substitutions at codon 83, SerLeu or Asp87Asn, Gly or Tyr. Alteration of Ser83 results in the most effective increase in the MIC of quinolone such as NAL and alterations of Asp87 result in the effective increase of MIC of fluoroquinolone. These results suggest that the continuous use of quinolones might induce the specific amino acid substitution at gyrA.
Amino Acid Substitution
;
Anti-Bacterial Agents
;
Ciprofloxacin
;
Codon
;
Escherichia coli*
;
Escherichia*
;
Fluoroquinolones
;
Gyeongsangbuk-do
;
Microbial Sensitivity Tests
;
Nalidixic Acid
;
Norfloxacin
;
Ofloxacin
;
Polymerase Chain Reaction
;
Quinolones
10.Two cases of a family of benign familial hematuria.
Byung Sik CHANG ; Pyung Kil KIM ; Soon Hee SUNG
Korean Journal of Nephrology 1993;12(4):677-681
No abstract available.
Hematuria*
;
Humans