1.The Study of Cell Killing Mechanism by Membrane Attack Complexes of Complement in the Nucleated Cells.
Sang Ho KIM ; Sung Hak PARK ; Myung Hoon CHUN
Korean Journal of Pathology 1992;26(3):253-269
The mechanism of cytolysis by complement attack of nucleated cells(NC) is of special interest in comparison to that of red blood cells. It is known that NC death by membrane attack comples, C5b-9, is caused by many factors, i.e., efficiency of complex assembly, activation of intrinsic metabolic pathway by signal transduction, cytotoxic effect of the channel itself and natural repair ability. These factors suggest that colloid osmotic lysis, known in red blood cells, does not fully explain the complement-mediated cell death of NC. In this study, the authors investigated correlation between biochemical and morphological changes to prove "Ca2+-mediated metabolic death"8~13) representing a mechanism of NC death caused by C5b-9 attack. The L1210 cells, mouse leukemic cell line carrying small complement channel(TAC5b-91) were used in the experiments. The amounts of intracellular adenine nucleotides to extracellular Ca2+, ouabain, KC1 and dextran were analyzed by bioluminescence method using luminometer. Cell viability was checked by 0.4% trypan blue dye and LDH release. Morphological observation of TAC5b-91 was done by immunocytochemical staining and electron microscope. The results were as follows: 1) The release of ATP, ADP and AMP followed by cell death was rapid and progressive along the incubation time at 37 degrees C and it was accelerated in 1.5 mM of [Ca2+]0. 2) There was no evidence of ATP repairment in the TAC5b-91. 3) Extracellular KC1(150 mM), dextran(0.66 mM) and ATP supplement(0.2 microM) could not effectively inhibit ATP depletion and cell death. Ouabain(27 and 100 microM) enhanced cell death and could not completely prevent ATP loss. 4) Most of the mitochondria showed swelling, loss of cristae and Ca2+ deposit in matrix in the electron microscopic observation. Rapid, sustained and irreversible depletion of adenine nucleotides was due to Ca2+ deposit with destruction of mitochondria and also the leakage through transmembrane channels. Moreover this energy depletion was accelerated by high extracellular Ca2+ concentration. These results indicate that Ca2+-mediated, energy exhaustion is one of the mechanisms of the metabolic cell death by C5b-9 attack of NC.
Mice
;
Animals
2.A COMPARISON OF MIDAZOLAM AND DIAZEPAM AS SEDATIVES FOR AMBULATORY PLASTIC SURGERY PATIENTS.
Yong Chan CHUN ; Hyun Cheol PARK ; Sung Ho CHOI
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(6):1274-1282
No abstract available.
Diazepam*
;
Humans
;
Hypnotics and Sedatives*
;
Midazolam*
;
Surgery, Plastic*
3.Prognostic indexes in patients treated with intravesical bacillus calmette-guerin for superficial bladder cancer.
Choal Hee PARK ; Chun Il KIM ; Sung Choon LEE
Journal of the Korean Cancer Association 1991;23(4):835-842
No abstract available.
Bacillus*
;
Humans
;
Urinary Bladder Neoplasms*
;
Urinary Bladder*
4.Schwannoma of the Adrenal Gland: A case report.
Yong Chan CHUN ; Sun Hee SUNG ; Chan Il PARK
Korean Journal of Pathology 1993;27(4):424-426
Retroperitoneum is often the site of occurrence of schwannoma, but reports on schwanoma of the adrenal gland is exceptional and only 4 cases have been documented in the literature. This report is to add one such case occurred in a 53 year-old male who had anorexia, nausea and indigestion for one month. Whole body bone scan and abdominal CT scan revealed a 10 cm sized solid mass at upper pole of the left kidney. Under the impression of renal cell carcinoma, an operation was performed. The tumor was well encapsulated and appeared not to involve the kidney. The cut surfaces were light yellow and seemed to be composed of several hard lobules with areas of mucoid, cystic and calcific changes. No adrenal gland was identified grossly. But microscopically, the tumor was found to be partly surrounded by a small portion of adrenal cortical tissue. Histologically the tumor was a typical schwannoma with Verocay bodies, although modified in some extents by mucoid degeneration, cystic change, hyaline change and focal calcification. It is worthwhile to remember that the retroperitoneal schwannoma commonly had a huge size, sometimes involving the adjacent structures.
Male
;
Humans
5.A Case of Solitary Keratoacanthoma Originated from Verruca Vulgaris.
Yoon Sung PARK ; Soo Il CHUN ; Chang Jo KOH
Korean Journal of Dermatology 1984;22(3):334-337
Keratoacanthoma is a benign neoplasm that develops as a protruding mass especially on the central part of face, We experienced a case of keratoacanthoma that developed on the nasal vestibule after the eradication of verruca vulgaris on the same site by surgical intervention 3 months before. We treated it with total excision and skin graft.
Keratoacanthoma*
;
Skin
;
Transplants
;
Warts*
6.A clinical comparison of surgical wound closured by nylon suture and adhesive skin tape.
Jung PARK ; Sung Hwan KIM ; Hong Joon CHUN
Journal of the Korean Surgical Society 1993;44(1):24-29
No abstract available.
Adhesives*
;
Nylons*
;
Skin*
;
Surgical Tape*
;
Sutures*
;
Wounds and Injuries*
7.Calcifications in the buttock
Ki Sung CHUN ; Seog Hee PARK ; Yong Whee BAHK
Journal of the Korean Radiological Society 1985;21(4):618-622
The majority of injections intended to be intramuscular are actually delivered into fat in the area of thebuttock in most of the patients having a thick gluteal fat layer. Injections of some drugs can cause tissuenecrosis that ensues in scar formation and often in dystrophic calcifiation giving rise to ring-like densities inantero-posterior radiographs of the pelvis. We studied the incidence and characteristics of calcifications in thebuttocks frequently noted in pelvic radiographs and whether they have any relationship with thickness anddistribution pattern of the fat layer in the buttocks. Pelvic radiograms of 220 consecutive patients (110 malesand 110 females) with the age ranging from 16 to 76 years(average 39) were reviewed. The area of buttock was divided arbitrarily into four quadrants by the vertical line crossing the center of the head of each femur and thehorizontal line connecting the summit of each femoral head, and the upper outer quadrant was further divided into four quadrants(Fig. 1). We measured the thickness of the extraperitoneal fat layer at the level where it cross theiliac crest bilaterally. The results were as follows; 1. Thirty out of 220 cases(14%) showed calcifications in thebuttock. 2. Calcifications in the buttock were much more frequent in female than in male (p<0.01). 3. Theincidence of calcifications increased with age(P<0.01) and with increase in fat layer thickness(P<0.01). 4,Calcifications in the buttock were mostly located at the upper outer quadrant of the buttock (78%). 5. Inconclusion, we assume that calcifications in the buttock are result of fat necrosis after injection into fatinstead of muscle.
Buttocks
;
Cicatrix
;
Fat Necrosis
;
Female
;
Femur
;
Head
;
Humans
;
Incidence
;
Male
;
Pelvis
8.Prenatal cytogenic study by midtrimester amniocentensis.
Sung Suk SEO ; Hae Ran HWANG ; Sung Sook JEON ; Chung Hee CHUN ; Sook Ja PARK
Korean Journal of Obstetrics and Gynecology 1993;36(9):3418-3425
No abstract available.
Female
;
Humans
;
Pregnancy
;
Pregnancy Trimester, Second*
9.A case of pompe disease associated with wpw syndrome.
Woo Sung CHUN ; Moon Sung PARK ; Se Wook OH ; Chang Joon KO ; Tai Seung KIM
Journal of the Korean Child Neurology Society 1993;1(1):179-185
No abstract available.
Glycogen Storage Disease Type II*
;
Wolff-Parkinson-White Syndrome*
10.A Case of Localized fibrous tumor of Pelvic Cavity.
Chun Ju LEE ; Byung Jin JANG ; Hyun Jun PARK ; Sung Yong KIM ; Hae Young PARK
Korean Journal of Urology 2001;42(1):124-126
Localized fibrous tumor is an uncommon submesothelial origin tumor found in pleura most commonly. Sixty five-year-old man with right lower quadrant pain was admitted. He was treated with resection of tumor and was diagnosed as localized fibrous tumor of pelvic cavity. He is now being followed up without any evidence of recurrence for 34 months. This case is presented with reviewing references.
Pleura
;
Recurrence