No abstract available.
Rabbits* ; Transplants* ; Zygoma*

No abstract available.
Free Tissue Flaps* ; Head* ; Neck*

No abstract available.
Skull Base* ; Skull*

No abstract available.
Gyeongsangbuk-do* ; Humans ; Retrospective Studies*

One consistent finding in the aging human RPE is the intracellular accumulation of lipofuscin Lipofuscin was applied to the cell culture technique to clarify the relationship between age related macular degeneration and lipofuscin accumulation in the human RPE. The results obtained in this study are as follows: 1. The initial suspensions of dissociated RPE cells consisted of densely pigmented and hexagonal cells. Rapid cell proliferation and confluency of a culture occured from 2 weeks after seeding. No pigment granule was observed in subcultured cells of 3rd passage. 2. When RPE cultures were subcultured the cells gradually de pigmented due to a redistribution of pigment granules. Quantitative analysis demonstrated that intensity of fluorescence for a certain number of cells reduced proportionally as the cell division proceeded. 3. Cultured human retinal pigment epithelial cells readily ingested lipofuscin isolated from human RPE cells. The amount of lipofuscin phagocytized by cultured RPE cells were greater than that phagocytized by aged cells. Accumulation and/or saturation of lipofuscin in the RPE cells at least in cell culture did not necessarily affect RPE cell survival.
Aging ; Cell Culture Techniques ; Cell Division ; Cell Proliferation ; Cell Survival ; Epithelial Cells ; Fluorescence ; Humans* ; Lipofuscin* ; Macular Degeneration ; Retinal Pigment Epithelium* ; Retinaldehyde* ; Suspensions

BACKGROUND: The common histopathology of Behget's disease is vasculitis associated with activation of neutrophils. The level of plasma elastase a 1 proteinase inhibitor (E a 1 PI), which represents the activation of neutrophils, may be a marker of Behcet's disease. OBJECTIVE: We examined the level of plasma elastase al proteinase inhibitor to evaluate the degree of neutrophil activation in Behcet's disease. METHOD: We measured plasma elastase a 1 proteinase inhibitor in 34 cases of untreated Behcet's disease patients and 30 cases of normal individuals by an enzyme immunoassay. We also studied the differences between the levels in two clinical types of Behcet's disease, the complete and incomplete type. RESULTS: The plasma level of elastase a 1 proteinase inhibitor was significantly higher in untreated Behcet's disease patients than in healthy controls. However, there was no significant difference between the levels in the two clinical types. CONCLUSION: These data suggest that the elevated level of plasma elastase a 1 proteinase inhibitor may reflect a state of chronic activation of neutrophils in Behcet's disease immunologically and further studies will be needed to evaluate the clinical status of Behcet's disease patients by measuring levels of plasma elastase a 1 proteinase inhibitor.
Humans ; Immunoenzyme Techniques ; Methods ; Neutrophil Activation ; Neutrophils ; Pancreatic Elastase ; Plasma* ; Vasculitis

Fifty patients with mild essential hypertension were studied to evaluate the efficacy of once-daily regimen of nitrendipine, 10~20mg daily for 12 weeks. 1) Thirty-Seven patients completed the study and showed -9% change in mean arterial headache etc. 2) Eight patients were dropped out because of side reaction, namely flushing, palpitation, headache etc. 3) Ambulatory blood pressure monitoring before and after treatment in 3 patients confirmed the drug efficacy revealing 9% decrement in mean blood pressure and 46% decrease in % elevated BP. 4) Twenty patients who were controlled with other hypotensive drugs were well controlled & maintained the blood pressure in normal range after switching to nitrendpine 10~20mg daily. In conclusion, citrendipine is a safe and good antihypertensive calcium antagonist in the treatment of mild hypertensives with 10~20mg of once-daily dosage.
Blood Pressure Monitoring, Ambulatory ; Blood Pressure* ; Calcium ; Flushing ; Headache ; Humans ; Hypertension ; Nitrendipine* ; Reference Values

Interferons(IFNs) exhibit an antiproliferative effect on many normal and transformed cells and have in vivo antitumor activity in a variety of cancers. Recent clinical studies have suggested major activity with IFNs, especially in advanced squamous cell carcinoma of the skin and cervix. With the objective of exploring how the IFNs might work in squamous carcinoma cell line, we studied the effect of IFN-a on cervical cancer cell lines. The effect of IFNs on apoptosis and cell cycle of cervical cancer cell lines(C33A, CaSki, SiHa, HeLa, ME-180) was analysed by flow cytometry in time dependent manner. Results were as follows: (1) According to cell count of studied cancer cell lines treated with 2,000 IU/ml IFN-a for 7 days exposure, IFN-a had the antiproliferative effect on all five tested cervical cancer cell lines. Also this antiproliferative effect was confirmed by WST-1 assay. (2) The effect of IFN-a on apoptosis of each cultute was analysed by flow cytometry after 3 days and 7 days exposure with 2,000 IU/ml IFN-a, Apoptosis was not induced by IFN-a treatment. (3) The effect of IFN-a on the cell cycle of each culture was analysed by flow cytometry after 3 days exposure with 2,000 IU/ml IFN-a. As compared to control cells, treatment with IFN-a resulted in a higher proportion of cells in S phase with lower portion of cells with G2/M phase. (4) Time course of IFN-a effect on HPV 16 and HPV 18 E6 mRNA levels was evaluated by northern blot analysis. In CaSki cell line, HPV 16 E6 mRNA expression induced by IFN-a was not inhibited. But in HeLa cell line, HPV 18 E6 inRNA expression was inhibited. IFN-a appears to have the antiproliferative effect on all five tested cervical cancer cell lines and the antiproliferative effect of IFN-a seemed to be induced not by apoptosis but by disruption on specific cell cycle. Also regulation of HPV E6 mRNA expression induced by IFN-a is not directly related to the mechanisms of the antiproliferative effect of IFN-a in cancer cell lines.
Apoptosis ; Blotting, Northern ; Carcinoma, Squamous Cell ; Cell Count ; Cell Cycle ; Cell Line* ; Cervix Uteri ; Female ; Flow Cytometry ; HeLa Cells ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans ; RNA, Messenger ; S Phase ; Skin ; Uterine Cervical Neoplasms*

The term 'orbital pseudotumor' has been used to describe inflammatory lesions of the orbital tissues of unknown etiology simulating in their clinical picture a neoplasm of the orbit. The term inflammatory pseudotumor of the orbit has a clinical rather than a pathological connotation. The clinical picture presented by inflammatory pseudotumor may resemble that of a true neoplasm in every way-there is proptosis which is irreducible, chemosis, swelling of the lid, limitation of mobility and frequently a discrete palpable mass. Usually recurrence has been Tare and several methods of treatment has been reported. A 48 year old Korean woman was found to have pseudotumor of her right orbit which was confirmed histopathologically on biopsy.
Biopsy ; Exophthalmos ; Female ; Granuloma, Plasma Cell ; Humans ; Middle Aged ; Orbit* ; Orbital Pseudotumor* ; Recurrence

The authers investigated the effct of liposome encapsulation on the pharmacokinetics of tobramycin after subconjuctival in jnjection in rabbits. Tobramycin was encapsulated into neutral liposomes of phosphatidylcholine and cholesterol. The final liposomal suspension contained tobramycin, 5mg/ml. One of the each rabbit received a subconjunctival injection(2.5mg/0.5ml) of liposome-encapsulated tobramycin or tobramycin alone. Tobramycin levels in the sclera measured 3 and 24 hours after injection were marked higher with the liposome-encapsulated drug than with the another preperation. The difference between liposomeencapsulated drug and the another preperation was not significant for cornea.
Cholesterol ; Cornea* ; Liposomes ; Pharmacokinetics ; Phosphatidylcholines ; Rabbits* ; Sclera* ; Tobramycin*