1.A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida) .
Sung Hee PARK ; Cheon Young CHANG ; Sung Shik SHIN
The Korean Journal of Parasitology 2005;43(4):141-147
A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of 22 x 40 mm in size was mounted at a height of 0.8 mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of copepods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to copepods during the handling process.
Protozoa
;
Male
;
Female
;
Culture Techniques/*methods
;
Culture Media
;
Copepoda/*growth & development
;
Animals
2.Considerations for Orthodontic Treatment in Elderly Patients.
Yang ho PARK ; Se hwan CHEON ; Sung soo SHIN ; Jun woo PARK ; Jun hyun AN
Journal of the Korean Geriatrics Society 2004;8(4):241-246
BACKGROUND: The growth of elderly population increased the need for oral health care. Elderly patients with poor teeth alignment needs more attention with orthodontic treatment METHODS: Elderly patients visiting department of orthodontics, Kangdong Sacred Heart Hospital between 2000-2004 were treated with fixed appliances in one or both dental arches. Treatment plans were different from than that of younger patients and included uncommon and strategic removals of teeth and prosthesis. RESULTS: There was decrease in orthodontic treatment forces with increasing age, and the observation made from this study was favorable in the patients' as well as in the orthodontist's, point of view. It was possible to move the remaining teeth considerably, and the retention was made with various fixed appliances. CONCLUSIONS: Orthodontic treatment is not limited by patient age. However, it is wise not to extend treatment goals too far beyond the patients' objective needs
Aged*
;
Dental Arch
;
Heart
;
Humans
;
Oral Health
;
Orthodontics
;
Prostheses and Implants
;
Tooth
3.Two Stage Reimplantation in Infected Total Knee Arthroplasty Using an Articulating Antibiotics Impregnated Cement Spacer: Surgical Technique.
Jae Cheon SIM ; Nam Sik CHUNG ; Ki Do HONG ; Sung Sik HA ; Sang Cheon AHN ; Sung Joon PARK
Journal of the Korean Knee Society 2005;17(1):15-21
PURPOSE: To evaluate the clinical result of two stage reimplantation of infected total knee arthroplasties, using an antibiotics impregnated articulating cement spacer. MATERIALS AND METHODS: Out of total 7 cases with infected total knee arthroplasties, 4 cases treated with two stage reimplantation, using an antibiotics impregnated articulating cement spacer were included in this study. After the treatment of infected total knee arthroplasties, the treatment of infection was evaluated by physical examination, radiologic studies, and hematologic studies. The results were evaluated using the Hospital for Special Surgery Knee Score (HSS). RESULTS: As results, 4 infected knee cases treated with two stage reimplantation did not have reinfection at the time of the follow up, and physical, laboratory, and radiologic studies were all within normal range. The range of motion of the reimplanted knees were from -5degrees in extension to 130 degrees in flexion, with an average of 117 degrees. The average score of the Hospital for Special Surgery Knee Score was 87. CONCLUSION: In conclusion, two stage arthroplasties, using antibiotics impregnated articulating cement spacer was an effective therapy not only for the treatment of the infection after the total knee arthroplasty but also effectiveness for recovery of the knee function.
Anti-Bacterial Agents*
;
Arthroplasty*
;
Follow-Up Studies
;
Knee*
;
Physical Examination
;
Range of Motion, Articular
;
Reference Values
;
Replantation*
4.Validity of the clinical recommendation of Hepatitis B virus vaccine to adults with negative HBsAg and AntiHBs.
Mi Kyeong OH ; Kyeong Soo CHEON ; Jong Sung KIM ; Byoung Kang PARK
Journal of the Korean Academy of Family Medicine 1997;18(10):1027-1034
BACKGROUND: Administration of hepatitis B vaccine has played a major part in the management of public health in this country. There were many researches to assess the immunogenecity, safety, long term effectiveness, dose, and route of administration of hepatitis B vaccine. But there was lack of validation of the recommendation that hepatit.is B vaccine should be administered in adults with negative HBsAg and AntiHBs, in an endemic area with high prevalence of HBsAg. Authors tried to establish a proper candidate for hepatitis B vaccination evaluating viral markers of hepatitis B in adults vaccinated. METHODS: 172 hepatitis B vaccinated subjects(35.0%) were drawn from 491 adults who have visited at health care center, Asan Kangnung Hospital from April 1st to May 31th, 1997. They were asked designed questions about the beginning year, frequency of vaccination, and the presence of family members with HBsAg in a direct line. RESULTS: Of the subjects(172), 2.3% were HBsAg(+), 63.4% AntiHBs(+), 68.6% AntiHBc(+). Among the HBsAg(-) adults(149) who had been vaccinated more than three times, 70.4%(105) were AntiHBs(+), of whom 66.6%(70) were AntiHBc(+) and 29.6%(44) were AntiHBs(-), of whom 56.8%(25) were AntiHBc(+). In the subjects(20) with family history of HBsAg, 80% were AntiHBc(+), which is higher than 61.2% in the group(129) without family history. Among the HBsAg(-) and AntiHBc(-) adults(54) who had been vaccinated more than three times, AntiHBs(+) group was not significantly differnt from AntiHBs(-) group in comparison of age and years after initial vaccination. CONCLUSIONS: The two thirds(68.9%) of vaccinated subjects showed AntiHBc(+), which means reflection of previous exposure to Hepatitis B virus. Accordingly, vaccination is not thought to be required for these. Consequently, all individuals are thought to have to be pretested for the presence of AntiHBc as well as HBsAg and AntiHBs in Korea.
Adult*
;
Biomarkers
;
Chungcheongnam-do
;
Delivery of Health Care
;
Hepatitis B Surface Antigens*
;
Hepatitis B Vaccines
;
Hepatitis B virus*
;
Hepatitis B*
;
Hepatitis*
;
Humans
;
Korea
;
Prevalence
;
Public Health
;
Vaccination
5.Perineal pagent's disease involving the inguinoscrotal area.
Jin Cheon KIM ; Kun Choon PARK ; Kyung Suck KOH ; Eun Sil YU ; Kyung Jeh SUNG
Journal of the Korean Cancer Association 1991;23(2):465-469
No abstract available.
7.Epidemiology and Clinical Characteristics of Enterovirus Infections in Children: A Single Center Analysis from 2006 to 2010.
In Soo PARK ; Hae Sung LEE ; Soo Han CHOI ; Hye Jin KIM ; Seo Yeon HWANG ; Doo Sung CHEON ; Jin Keun CHANG
Korean Journal of Pediatric Infectious Diseases 2013;20(2):81-88
PURPOSE: This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a secondary hospital during recent 5 years. METHODS: We collected the cerebrospinal fluid, stool and throat swab samples from the pediatric patients with suspected EV infections in KEPCO Medical Center, Seoul, Korea from July 2006 to September 2010. EV detection and genotype identification were performed by RT-PCR at Korea Centers for Disease Control and Prevention. RESULTS: A total of 386 samples were collected from 277 patients during study period. Ninety-eight patients (35.4%) were diagnosed with EV infections. The RT-PCR positive rate was the highest in throat swab samples (48.3%). The median age of patient was 4.7 years (range, 0.1-12.5 years). Aseptic meningitis (50, 51.0%) was the most common clinical manifestation; herpangina (22, 22.4%) and hand-foot-mouth disease (18, 18.4%). One hundred EVs were isolated from 98 patients and 20 genotypes of EV were identified; Echovirus 30 (28 cases, 28%), Enterovirus 71 (12 cases, 12%), Echovirus 25 (10 cases, 10%), Echovirus 9 (9 cases, 9%) and Coxsackievirus A6 (8 cases, 8%). Aseptic meningitis caused by Echovirus 30 was the most common manifestation in 2008. There was no complicated case caused by Enterovirus 71. CONCLUSION: This study showed the epidemiology of confirmed EV infection in children from 2006 to 2010. There is a need for continuous surveillance of EV infections and its clinical manifestations.
Centers for Disease Control and Prevention (U.S.)
;
Child
;
Echovirus 9
;
Enterovirus
;
Enterovirus B, Human
;
Enterovirus Infections
;
Genotype
;
Herpangina
;
Humans
;
Korea
;
Meningitis, Aseptic
;
Pharynx
8.Ocular Torsion and Tilt of Subjective Visual Vertical and Head in Patients with Acute Brainstem Stroke.
Sung Eun CHO ; Jeong Hyuk PARK ; Kyung Cheon CHUNG ; Dae il CHANG
Journal of the Korean Neurological Association 1998;16(1):15-20
BACKGROUND & PURPOSE: The ocular torsion (OT) and tilt of the subjective visual vertical (SVV) are sensitive brainstem signs and helps us to localize lesion. We calibrated the degree of OT, SVV tilt and head tilt to investigate their characteristics and temporal profiles in patients with brainstem lesion. METHODS: We selected 15 patients with acute brainstem stroke. We took serial fundus photographs and body pictures in upright position at various times after the stroke. We also determined the deviations of patient's SVV. The data measurement for this investigation ranged from day 2 to day 47. RESULTS: Eight of ten patients with lateral medullay infarction showed ipsiversive tilt of SVV and OT. Among four patients with pontine infarction, one showed ipsiversive tilt and three contraversive. One patient with midbrain hemorrhage showed cotraversive tilt. The resolution of OT and the tilt of SVV in medullary lesions occurred over the periods ranging from 7 days to more than 47 days and was slower in patients with upper brainstem lesion than lower brainstem lesion. The directions of head tilt in our patients, especially with medullary lesions, were rather different from the previous reports. CONCLUSION: The vestibular dysfunction by the brainstem lesions disturbs the eye and head stabilization and also distorts the perception of the subjective vertical in space. The direction of OT and the tilt of SVV is ipsiversive in lower brainstem lesion and contraversive in upper brainstem lesion. But head tilt is contraversive in most lower brainstem lesion as well as upper brainstem lesion. Large-grouped study for the direction of head tilt is thought to be needed. These signs are compensated in the course of clinical recovery.
Brain Stem Infarctions*
;
Brain Stem*
;
Head*
;
Hemorrhage
;
Humans
;
Infarction
;
Mesencephalon
;
Stroke
9.Combined Spinal Epidural Anesthesia for Cesarean Section.
Dong Hee KIM ; Sung Hee KIM ; Seok Kon KIM ; Nam Hoon PARK
Korean Journal of Anesthesiology 1996;30(3):333-338
BACKGROUND: The present study was designed to develop a combined spinal epidural (CSE) anesthesia using single segment technique(SST) for cesarean section. We attempted to find the most suitable spinal and epidural local anesthetic doses providing high quality of surgical analgesia and minimal side effects during CSE anesthesia. METHODS: 40 patients scheduled for elective cesarean section under the CSE technique were randomly divided into four groups. 2.5mg(Group 1), 5mg(Group 2), 7.5mg(Group 3) and 10mg(Group 4) of 0.5% hyperbaric bupivacaine was injected into the subarachnoid space through a 26-gauge long Quincke needle. If the block did not reach the T4 level in 15min., it was extended by fractionated doses of 2% lidocaine with 1:200,000 epinephrine administered through the epidural catheter. RESULTS: All patients in Group 1, 2 and 3 needed epidural lidocaine, 21.0+/-0.8 ml(Group 1), 11.1+/-0.6 ml(Group 2) and 7.4+/-0.7 ml(Group 3). Anesthesia in Group 4 was mostly due to spinal block. Group 2 and 3 resulted in satisfactory anesthesia with rapid onset, good surgical analgesia and muscle relaxation and minimal side effects. Group 1 provided insufficient muscle relaxation and Group 4 had higher incidence(60%) of maternal hypotension than other groups. CONCLUSIONS: The CSE technique, using 5mg or 7.5mg of subarachnoid bupivacaine and with sufficient epidural lidocaine to reach a T4 level, had the advantages of both spinal and epidural anesthesia with few of the complications of either.
Analgesia
;
Anesthesia
;
Anesthesia, Epidural*
;
Anesthetics
;
Bupivacaine
;
Catheters
;
Cesarean Section*
;
Epinephrine
;
Female
;
Humans
;
Hypotension
;
Lidocaine
;
Muscle Relaxation
;
Needles
;
Pregnancy
;
Subarachnoid Space
10.Consecutive Reversible Changes of Peripheral Nerve Conduction in Tetrodotoxification.
Jong Ho PARK ; Sung Min KIM ; Jae Cheon BAE ; Ki Han KWON ; Byung Chul LEE ; Harry NA
Journal of the Korean Geriatrics Society 1999;3(2):102-107
Tetrodotoxin (TTX) cause neurologic dysfunction by blocking the voltage-gated sodium channels located in all of the peripheral nerves and muscles. We experienced two patients presenting with generalized motor weakness after ingestion of pufferfish. The nerve conduction study showed diffuse slowing of motor and sensory nerve conduction velocity, prolonged motor terminal latency and decreased sensory nerve action potentials without temporal dispersion or conduction block. Abnormal findings of nerve conduction study improved rapidly without any deterioration. Clinical symptoms and signs ameliorated in accordance with findings of nerve conduction study without any neurologic sequelae. These imply that tetrodotoxication is reversible and functional neurologic disorder. We suggest that nerve conduction studies can be available in serial monitoring of tetrodotoxication as an objective means.
Action Potentials
;
Eating
;
Humans
;
Muscles
;
Nervous System Diseases
;
Neural Conduction
;
Neurologic Manifestations
;
Peripheral Nerves*
;
Tetraodontiformes
;
Tetrodotoxin
;
Voltage-Gated Sodium Channels